Abstract：
:We have identified a novel mouse gene named pancortin that is expressed dominantly in the mature cerebral cortex. This gene produces four different species of proteins, Pancortin-1-4, sharing a common region in the middle of their structure with two variations at the N-terminal (A1 or A2 part) and C-terminal (C1 or C2 part) sides, respectively. In the present study, we showed that expression of mRNAs for A2-Pancortins (Pancortin species that contain the A2 part, i.e., Pancortin-3 and -4) is more dominant than that of mRNAs for A1-Pancortins (Pancortin species that contain the A1 part, i.e., Pancortin-1 and -2) in the prenatal mouse cerebral neocortex. Using western blot analysis, we found that substantial amounts of both A2-Pancortins were present in the prenatal cerebral neocortex and P19 cells after inducing neuronal differentiation. A2-Pancortins were still present in the cerebral neocortex of the adult, although their mRNAs were hardly detected. In contrast, the amount of A1-Pancortins did not increase after the third postnatal week in spite of their intense gene expression. Furthermore, we showed that recombinant Pancortin-3, one of the A2-Pancortins, was a secreted protein, in contrast to Pancortin-1 (one of the A1-Pancortins). These results suggest that A2-Pancortins are extracellular proteins essential for neuronal differentiation and that their molecular behavior is distinct from that of A1-Pancortins.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Nagano T,Nakamura A,Konno D,Kurata M,Yagi H,Sato Mdoi
10.1046/j.1471-4159.2000.0750001.xkeywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
1-8issue
1eissn
0022-3042issn
1471-4159journal_volume
75pub_type
杂志文章abstract：:The effect of the glutamine synthetase (GS) inhibitor, methionine sulfoximine (MSO), on glutamate levels in, and glutamate release from, rat striatal tissue was examined. Tissue levels of glutamate were unchanged 24 h after an intraventricular injection of MSO, but tissue glutamine levels were decreased 50%. Calcium-d...
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.2008.05614.x
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更新日期：2003-03-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1981.tb06336.x
更新日期：1981-12-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：1981-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1984.tb02792.x
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pub_type： 杂志文章,评审
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更新日期：1993-07-01 00:00:00
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journal_title：Journal of neurochemistry
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更新日期：1996-02-01 00:00:00
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更新日期：1988-08-01 00:00:00
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journal_title：Journal of neurochemistry
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更新日期：2009-08-01 00:00:00
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