Dimethylsulfoxide reductase: an enzyme capable of catalysis with either molybdenum or tungsten at the active site.

abstract：:The Neurospora crassa CYT-18 protein is a mitochondrial tyrosyl-tRNA synthetase that also promotes self-splicing of group I intron RNAs by stabilizing the functional structure in the conserved core. CYT-18 binds the core along the same surface as a common peripheral element, P5abc, suggesting that CYT-18 can replace P...

journal_title：Journal of molecular biology

authors： Chadee AB,Bhaskaran H,Russell R

更新日期：2010-01-22 00:00:00

abstract：:Small nuclear ribonucleoproteins (snRNPs) containing U1 and U5 snRNAs from HeLa cells have been fractionated using a combination of isopycnic centrifugation in cesium chloride and ion-exchange chromatography on DEAE-Sepharose. The procedure is based on the extreme stability conferred upon snRNPs by Mg2+ enabling them ...

journal_title：Journal of molecular biology

authors： Lelay-Taha MN,Reveillaud I,Sri-Widada J,Brunel C,Jeanteur P

更新日期：1986-06-05 00:00:00

abstract：:Sirtuins are NAD+-dependent protein deacetylase enzymes that are broadly conserved from bacteria to human, and have been implicated to play important roles in gene regulation, metabolism and longevity. cobB is a bacterial sirtuin that deacetylates acetyl-CoA synthetase (Acs) at an active site lysine to stimulate its e...

journal_title：Journal of molecular biology

authors： Zhao K,Chai X,Marmorstein R

更新日期：2004-03-26 00:00:00

abstract：:Restriction fragments of bacteriophage lambda DNA corresponding to the major leftward transcription unit were purified, denatured to form single-stranded DNA, self-annealed, and examined by electron microscopy. Three intrastrand stem and loop secondary structures were observed reproducibly and the locations of the pai...

journal_title：Journal of molecular biology

authors： Edlind TD,Cooley TE,Richards SH,Ihler GM

更新日期：1984-11-05 00:00:00

abstract：:Despite proline being assumed to be a helix-breaker, a large number of alpha-helices are found to contain Pro in globular as well as membrane proteins. Proline has no free NH group and therefore cannot form the conventional intra-helical NH.O=C hydrogen bond. An analysis of known protein structures has shown that the ...

journal_title：Journal of molecular biology

authors： Chakrabarti P,Chakrabarti S

更新日期：1998-12-11 00:00:00

abstract：:The type A domain of the von Willebrand Factor is found also in the complement proteins factor B (FB), C2, CR3 and CR4, the integrins, collagen types VI, VII, XII and XIV, and other proteins. FB is a component of the alternative pathway of the complement system of immune defence, and is cleaved into the fragments Bb a...

journal_title：Journal of molecular biology

authors： Perkins SJ,Smith KF,Williams SC,Haris PI,Chapman D,Sim RB

更新日期：1994-04-22 00:00:00

abstract：:Many essential biological processes are mediated by complex molecular machines comprising multiple subunits. Knowledge on the architecture of individual subunits and their positions within the overall multimeric complex is key to understanding the molecular mechanisms of macromolecular assemblies. The anaphase-promoti...

journal_title：Journal of molecular biology

authors： Cronin NB,Yang J,Zhang Z,Kulkarni K,Chang L,Yamano H,Barford D

更新日期：2015-10-09 00:00:00

abstract：:In human metallothionein-2, the exchange rate constants of ten amide protons were found to range from 1.7 x 10(-4) to 1 x 10(-1) min-1 at pH 6.3 and 8 degrees C. Most of these slowly exchanging protons could be associated with hydrogen bonds in secondary structure elements of the alpha-domain. Amide proton exchange ra...

journal_title：Journal of molecular biology

authors： Messerle BA,Bos M,Schäffer A,Vasák M,Kägi JH,Wüthrich K

更新日期：1990-08-05 00:00:00

abstract：:Two site-directed mutants of Escherichia coli DNA helicase II (UvrD) were constructed to examine the functional significance of motif VI in a superfamily I helicase. Threonine 604 and arginine 605, representing two of the most highly conserved residues in motif VI, were replaced with alanine, generating the mutant all...

journal_title：Journal of molecular biology

authors： Hall MC,Ozsoy AZ,Matson SW

更新日期：1998-03-27 00:00:00

abstract：:eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-...

journal_title：Journal of molecular biology

authors： Wei Z,Xue Y,Xu H,Gong W

更新日期：2006-05-26 00:00:00

abstract：:In vivo, proteins occur in widely different physio-chemical environments, and, from in vitro studies, we know that protein structure can be very sensitive to environment. However, theoretical studies of protein structure have tended to ignore this complexity. In this paper, we have approached this problem by grouping ...

journal_title：Journal of molecular biology

authors： Andrade MA,O'Donoghue SI,Rost B

更新日期：1998-02-20 00:00:00

abstract：:Heme is an essential prosthetic group of proteins involved in oxygen transport, energy metabolism and nitric oxide production. ALAS1 (5-aminolevulinate synthase) is the rate-limiting enzyme in heme synthesis in the liver and is highly regulated to adapt to the metabolic demand of the hepatocyte. In the present study, ...

journal_title：Journal of molecular biology

authors： Degenhardt T,Väisänen S,Rakhshandehroo M,Kersten S,Carlberg C

更新日期：2009-05-01 00:00:00

abstract：:In order to understand the contribution of hydrophobic residues to the conformational stability of human lysozyme, five Ile mutants (Ile --> Val) in the interior of the protein were constructed. The thermodynamic parameters characterizing the denaturation of these mutant proteins were determined by scanning calorimetr...

journal_title：Journal of molecular biology

authors： Takano K,Ogasahara K,Kaneda H,Yamagata Y,Fujii S,Kanaya E,Kikuchi M,Oobatake M,Yutani K

更新日期：1995-11-17 00:00:00

abstract：:Bacteriophage P1 initiates the processive packaging of its DNA at a unique site called pac. We show that a functional pac site is contained within a 161 base-pair segment of P1 EcoRI fragment 20. It extends from a position 71 base-pairs to a position 232 base-pairs from the EcoRI-22 proximal side of that fragment. The...

journal_title：Journal of molecular biology

authors： Sternberg N,Coulby J

更新日期：1987-04-05 00:00:00

abstract：:Post-translational modifications (PTMs) of histone proteins play a crucial role in the regulation of chromatin structure and functions. Studies in the last few decades have revealed the significance of histone PTMs in key cellular processes including DNA replication, repair, transcription, apoptosis and cell cycle reg...

journal_title：Journal of molecular biology

authors： Azad GK,Swagatika S,Kumawat M,Kumawat R,Tomar RS

更新日期：2018-09-14 00:00:00

abstract：:Here, we used data of complete genomes to study comparatively the metabolism of different species. We built phenetic trees based on the enzymatic functions present in different parts of metabolism. Seven broad metabolic classes, comprising a total of 69 metabolic pathways, were comparatively analyzed for 27 fully sequ...

journal_title：Journal of molecular biology

authors： Aguilar D,Aviles FX,Querol E,Sternberg MJ

更新日期：2004-07-09 00:00:00

abstract：:Peptide deformylase (PDF) has received considerable attention during the last few years as a potential target for a new type of antibiotics. It is an essential enzyme in eubacteria for the removal of the formyl group from the N terminus of the nascent polypeptide chain. We have solved the X-ray structures of four memb...

journal_title：Journal of molecular biology

authors： Kreusch A,Spraggon G,Lee CC,Klock H,McMullan D,Ng K,Shin T,Vincent J,Warner I,Ericson C,Lesley SA

更新日期：2003-07-04 00:00:00

abstract：:Mutations at solvent-inaccessible core positions in proteins can impact function through many biophysical mechanisms including alterations to thermodynamic stability and protein dynamics. As these properties of proteins are difficult to investigate, the impacts of core mutations on protein function are poorly understo...

journal_title：Journal of molecular biology

authors： Lee SY,Pullen L,Virgil DJ,Castañeda CA,Abeykoon D,Bolon DN,Fushman D

更新日期：2014-04-03 00:00:00

abstract：:A structural investigation of the sodium dodecyl sulfate (SDS)-induced fibrillation of alpha-synuclein (alphaSN), a 140-amino-acid protein implicated in Parkinson's disease, has been performed. Spectroscopic analysis has been combined with isothermal titration calorimetry, small-angle X-ray scattering, and transmissio...

journal_title：Journal of molecular biology

authors： Giehm L,Oliveira CL,Christiansen G,Pedersen JS,Otzen DE

更新日期：2010-08-06 00:00:00

abstract：:The folding pathway of human FKBP12, a 12 kDa FK506-binding protein (immunophilin), has been characterised. Unfolding and refolding rate constants have been determined over a wide range of denaturant concentrations and data are shown to fit to a two-state model of folding in which only the denatured and native states ...

journal_title：Journal of molecular biology

authors： Main ER,Fulton KF,Jackson SE

更新日期：1999-08-13 00:00:00

abstract：:The structure of the Escherichia coli gene coding for the metabolically stable 4.5 S RNA has been determined by cloning and DNA sequence analysis. Results from Southern hybridization assays carried out prior to cloning show the 4.5 S DNA to be limited to a single locus in the E. coli K12 genome. A 5.4 X 10(3) base DNA...

journal_title：Journal of molecular biology

authors： Hsu LM,Zagorski J,Fournier MJ

更新日期：1984-09-25 00:00:00

abstract：:The amphipathic alpha-helix is a recurrent feature of membrane-active proteins, peptides, and toxins. Despite extensive biophysical studies, the structural details of its affinity for membrane interfaces remain rather vague. We report here the first results of an effort to obtain detailed structural information about ...

journal_title：Journal of molecular biology

authors： Hristova K,Wimley WC,Mishra VK,Anantharamiah GM,Segrest JP,White SH

更新日期：1999-07-02 00:00:00

abstract：:SpoIIAA is a key component in the network of interactions that regulate the first sporulation-specific transcription factor, sigma(F), in Bacillus subtilis. In its unphosphorylated form SpoIIAA is either phosphorylated by or forms a non-covalent complex with SpoIIAB, whereas in its phosphorylated form it is dephosphor...

journal_title：Journal of molecular biology

authors： Clarkson J,Campbell ID,Yudkin MD

更新日期：2001-11-30 00:00:00

abstract：:The recent growth in protein databases has revealed the functional diversity of many protein superfamilies. We have assessed the functional variation of homologous enzyme superfamilies containing two or more enzymes, as defined by the CATH protein structure classification, by way of the Enzyme Commission (EC) scheme. ...

journal_title：Journal of molecular biology

authors： Todd AE,Orengo CA,Thornton JM

更新日期：2001-04-06 00:00:00

abstract：:The active site of influenza virus neuraminidase (NA) is formed by 11 universally conserved residues. A guanidino group incorporated into two unrelated NA inhibitors was previously reported to occupy different negatively charged sites in the NA active site, A new inhibitor containing two guanidino groups was synthesiz...

journal_title：Journal of molecular biology

authors： Sudbeck EA,Jedrzejas MJ,Singh S,Brouillette WJ,Air GM,Laver WG,Babu YS,Bantia S,Chand P,Chu N,Montgomery JA,Walsh DA,Luo M

更新日期：1997-04-04 00:00:00

abstract：:Automated protein redesign, as implemented in the program ORBIT, was used to redesign the core of phage T4 lysozyme. A total of 26 buried or partially buried sites in the C-terminal domain were allowed to vary both their sequence and side-chain conformation while the backbone and non-selected side-chains remained fixe...

journal_title：Journal of molecular biology

authors： Mooers BH,Datta D,Baase WA,Zollars ES,Mayo SL,Matthews BW

更新日期：2003-09-19 00:00:00

abstract：:We report the results of an NMR study of unlabeled and uniformly (13)C,(15)N-labeled d(G(3)AG(2)T(3)G(3)AT) in 100 mM NaCl, conditions under which it forms a dimeric quadruplex containing several new topological features. The DNA oligomer chain in each symmetry-related monomer subunit undergoes three sharp turns to fo...

journal_title：Journal of molecular biology

authors： Zhang N,Gorin A,Majumdar A,Kettani A,Chernichenko N,Skripkin E,Patel DJ

更新日期：2001-08-31 00:00:00

abstract：:The discovery that RNA molecules can fold into complex structures and carry out diverse cellular roles has led to interest in developing tools for modeling RNA tertiary structure. While significant progress has been made in establishing that the RNA backbone is rotameric, few libraries of discrete conformations specif...

journal_title：Journal of molecular biology

authors： Humphris-Narayanan E,Pyle AM

更新日期：2012-08-03 00:00:00

abstract：:Despite the condensed nature of terminal sequences, the telomeres are transcribed into a group of noncoding RNAs, including the TElomeric Repeat-containing RNA (TERRA). Since the discovery of TERRA, its evolutionary conserved function has been confirmed, and its involvement in telomere length regulation, heterochromat...

journal_title：Journal of molecular biology

authors： Coulon S,Vaurs M

更新日期：2020-07-10 00:00:00

abstract：:R2 elements are non-long terminal repeat (non-LTR) retrotransposons with a single open reading-frame encoding reverse transcriptase, DNA endonuclease and nucleic acid-binding domains. The elements are specialized for insertion into the 28 S rRNA genes of many animal phyla. The R2-encoded activities initiate retrotrans...

journal_title：Journal of molecular biology

authors： Kurzynska-Kokorniak A,Jamburuthugoda VK,Bibillo A,Eickbush TH

更新日期：2007-11-23 00:00:00