当前位置： SCI文献检索 > EXPERIMENTAL HEMATOLOGY期刊下所有文献 > Molecular analysis of the hematopoiesis supporting osteoblastic cell line U2-OS.

Molecular analysis of the hematopoiesis supporting osteoblastic cell line U2-OS.

Abstract：

OBJECTIVE:Osteoblasts play an important role in regulating hematopoiesis in the bone marrow. Here we show that U2-OS, a widely used osteoblastic cell line derived from an osteosarcoma, has the capacity to support proliferation of human hematopoietic progenitor cells in vitro. In this study, U2-OS cells are characterized at the molecular level to unravel the molecular mechanisms underlying the support of hematopoiesis. MATERIALS AND METHODS:U2-OS was analyzed in great detail using RT-PCR and flow cytometry. In addition, a cDNA library was constructed and randomly sequenced to obtain insight in the repertoire of expressed molecules. RESULTS:A broad panel of growth factors and cytokines is expressed by U2-OS. TGF-beta, GM-CSF, c-kit ligand, and IL-7 are produced constitutively and IL-1beta, IL-6, IL-8, TNF-alpha, IFN-gamma, and MIP1-alpha are upregulated upon stimulation. In addition to those, mRNAs of the CC chemokine LARC and leukemia inhibitory factor were identified. U2-OS cells express high levels of beta1-integrins at the cell surface: VLA-2, VLA-3, VLA-4, VLA-5, VLA-6, and the integrin alphavbeta3. Besides integrins, ALCAM and NCAM are detected on the cell surface of U2-OS. Interestingly, we show that CD34(+) progenitor cells expressing ALCAM are highly proliferative when compared with CD34(+) ALCAM(low) cells, hinting at a role for ALCAM in anchoring progenitor cells to the bone marrow stroma. Interestingly, random sequencing of an U2-OS cDNA library yielded almost 10% of novel cDNAs with a potential role in hematopoiesis. The involvement of these novel molecules in hematopoiesis is an interesting target for future investigations. CONCLUSIONS:We conclude that U2-OS supports outgrowth of hematopoietic progenitor cells and accordingly expresses adhesion molecules and growth factors and a number of novel, as yet uncharacterized potentially interesting genes.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

Nelissen JM,Torensma R,Pluyter M,Adema GJ,Raymakers RA,van Kooyk Y,Figdor CG

doi

10.1016/s0301-472x(00)00127-2

keywords:

subject

Has Abstract

pub_date

2000-04-01 00:00:00

pages

422-32

issue

eissn

0301-472X

issn

1873-2399

pii

S0301-472X(00)00127-2

journal_volume

pub_type

杂志文章

在线工具

Molecular analysis of the hematopoiesis supporting osteoblastic cell line U2-OS.