当前位置： SCI文献检索 > EXPERIMENTAL HEMATOLOGY期刊下所有文献 > A NUP98-HOXD13 leukemic fusion gene leads to impaired class switch recombination and antibody production.

A NUP98-HOXD13 leukemic fusion gene leads to impaired class switch recombination and antibody production.

Abstract：

:Myelodysplastic syndrome is a clonal process characterized by ineffective hematopoiesis and progression to acute leukemia. Although many myelodysplastic syndrome and leukemic patients have compromised immunity, the role of underlying mutations in regulating immune function is poorly understood. Recent studies show that NUP98-HOXD13 (NHD13) fusion gene results in myelodysplastic syndrome and impairs lymphocyte differentiation in transgenic mice. In our studies, we sought to elucidate the mechanism by which NHD13 affects B-lymphocyte development and function. Based on our preliminary findings that transgenic mice had increased levels of IgM and reduced IgG1 and IgE, we hypothesized that the fusion gene might impair class switch recombination (CSR). Mice were immunologically challenged with dinitrophenol. NHD13 mice showed a marked reduction in B-lymphocyte differentiation in their bone marrow and spleen following dinitrophenol stimulation and had reduced production of dinitrophenol-specific antibodies. Spleen follicles from these mice were small and hypocellular, indicating failure of clonal expansion. When isolated NHD13 B lymphocytes were stimulated in vitro using Escherichia coli lipopolysaccharide or lipopolysaccharide + interleukin-4, they failed to undergo sufficient CSR and proliferation. Taken together, our findings show that expression of NUP98-HOXD13 impairs CSR and reduces the antibody-mediated immune response, in addition to its role in leukemia. Further delineation of the NUP98-HOXD13 transgene may reveal novel pathways involved in CSR.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

Puthiyaveetil AG,Heid B,Reilly CM,HogenEsch H,Caudell DL

doi

10.1016/j.exphem.2012.05.002

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

622-33

issue

8

eissn

0301-472X

issn

1873-2399

pii

S0301-472X(12)00177-4

journal_volume

40

pub_type

杂志文章

相关文献

EXPERIMENTAL HEMATOLOGY文献大全
  • Stimulation of megakaryocytopoiesis in mice by human modified C-reactive protein (mCRP)

    abstract：:The prototypic human acute phase reactant, C-reactive protein (CRP), and a structurally modified form of CRP (mCRP) were studied as agents which could stimulate thrombopoiesis in both in vitro and in vivo mouse models. mCRP, but not the widely studied (native) pentameric form of CRP, demonstrated significant megakaryo...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Potempa LA,Motie M,Wright KE,Crump BL,Radosevich JA,Sakai N,Lai G,Tanaka K,Kojima E,Tsuboi A

    更新日期：1996-02-01 00:00:00

  • Stem cell activity of porcine c-kit+ hematopoietic cells.

    abstract：OBJECTIVE:A marker for hematopoietic stem cells (HSCs) of pigs, which are considered to be the most suitable donors for clinical xenotransplantation, has not yet been identified. In this study, we examined the HSC activity of porcine c-kit+ bone marrow cells (BMCs). METHODS:The HSC activity of porcine c-kit+ BMCs was ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(03)00197-8

    authors： Le Guern AC,Giovino MA,Abe M,Theodore PR,Qi J,Down JD,Sachs DH,Sykes M,Yang YG

    更新日期：2003-09-01 00:00:00

  • HSP27 protects AML cells against VP-16-induced apoptosis through modulation of p38 and c-Jun.

    abstract：OBJECTIVE:To investigate 1) the signal transduction pathways affected by heat shock protein 27 (HSP27) expression; and 2) the expression and regulation of HSP27 in acute myeloid leukemia (AML). MATERIALS AND METHODS:RNA interference studies for HSP27 in leukemic TF-1 cells were used to investigate the effects on downs...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2005.03.009

    authors： Schepers H,Geugien M,van der Toorn M,Bryantsev AL,Kampinga HH,Eggen BJ,Vellenga E

    更新日期：2005-06-01 00:00:00

  • Assessment of proliferative and colony-forming capacity after successive in vitro divisions of single human CD34+ cells initially isolated in G0.

    abstract：:Exit of primitive hematopoietic progenitor cells (HPCs) from the G0 phase of the cell cycle in response to in vitro cytokine stimulation is a limiting step in successful ex vivo expansion. Simultaneous DNA/RNA staining with Hoechst 33342 and pyronin Y was used to separate human bone marrow CD34+ cells residing in G0 (...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Gothot A,Pyatt R,McMahel J,Rice S,Srour EF

    更新日期：1998-07-01 00:00:00

  • Specificity of hemin action in vivo at early stages of hematopoietic cell differentiation.

    abstract：:The effects of a single injection of hemin on murine marrow BFU-E and CFU-S were assessed to determine whether hemin is as effective in augmenting primitive (day 7) BFU-E levels in situ as it is in vitro and to assess hemin's action on transplantable pluripotent stem cells (CFU-S). The results show that hemin exerts a...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Monette FC,Holden SA,Sheehy MJ,Matzinger EA

    更新日期：1984-11-01 00:00:00

  • Tumor necrosis factor p55 receptor (TNF-RI) mediates the in vitro inhibition of hepatic erythropoietin production.

    abstract：:Tumor necrosis factor alpha (TNFalpha) is thought to contribute to the blunted erythropoietin (Epo) production in inflammatory diseases. The present study was carried out to find out as to whether the 55 kD (TNF-RI) or the 75 kD (TNF-RII) receptor is responsible for the TNFalpha-induced inhibition of hepatic Epo synth...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(98)00054-x

    authors： Jelkmann W,Hellwig-Buergel T

    更新日期：1999-02-01 00:00:00

  • Trochanter bone marrow: a source of normal human colony forming cells.

    abstract：:In vitro studies of human hemopoiesis are often limited by the availability of normal bone marrow. We have overcome this difficulty by taking advantage of the bone marrow fragments removed during total hip replacement. We report here a comparative study of the colony forming capacity of trochanter and sternal or iliac...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： de Laforest PG,Rio B,Triebel F,Lasmayous-Riou N,Clarac JP,Tanzer J

    更新日期：1979-07-01 00:00:00

  • Effects of androgen on transient endogenous spleen colonies and other hemopoietic stem cells in mice.

    abstract：:To clarify the effects of androgen on hemopoiesis in mice, we investigated changes in hemopoietic stem cells at various stages of differentiation after injection of 19-nandrolone decanoate (19-ND). 19-ND induced (a) a marked increase in the population of CFUe, especially in the spleen, and (b) a less significant incre...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Kamamoto T,Sakoda H,Yoshida Y,Uchino H,Imai N,Mori KJ

    更新日期：1985-08-01 00:00:00

  • Treating leukemia at the risk of inducing severe anemia.

    abstract：:Anemia is a frequently observed adverse effect in cancer patients who receive chemotherapy or drugs designed to block specific oncogenic signaling pathways, although the underlying mechanisms are poorly understood. An article first published online (Zhu HH, Luo X, Zhang K, et al. Proc Natl Acad Sci USA 2015;112:13342-...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2016.01.004

    authors： Chen WS,Zhu HH,Feng GS

    更新日期：2016-05-01 00:00:00

  • Hepatocyte growth factor/scatter factor (HGF/SF) is produced by human bone marrow stromal cells and promotes proliferation, adhesion and survival of human hematopoietic progenitor cells (CD34+).

    abstract：:The fate of hematopoietic progenitor cells (HPCs) in the bone marrow (BM) microenvironment is determined by two different interactions: 1) they adhere (via integrins) to both extracellular matrix molecules and BM stromal cells; and 2) stromal cells produce cytokines that influence their survival, proliferation, differ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Weimar IS,Miranda N,Muller EJ,Hekman A,Kerst JM,de Gast GC,Gerritsen WR

    更新日期：1998-08-01 00:00:00

  • Agonists to the A3 adenosine receptor induce G-CSF production via NF-kappaB activation: a new class of myeloprotective agents.

    abstract：OBJECTIVE:The aim of this study was to evaluate the effect of CF101, a synthetic agonist to the A3 adenosine receptor (A3AR), on the production of granulocyte colony-stimulating factor (G-CSF). The ability of CF101 to act as a myeloprotective agent in chemotherapy-treated mice was tested. METHODS:CF101 was administere...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(02)00962-1

    authors： Bar-Yehuda S,Madi L,Barak D,Mittelman M,Ardon E,Ochaion A,Cohn S,Fishman P

    更新日期：2002-12-01 00:00:00

  • Human acute myeloid leukemia blast-derived exosomes in patient-derived xenograft mice mediate immune suppression.

    abstract：:Exosomes are virus-size membrane-bound vesicles of endocytic origin present in all body fluids. Plasma of AML patients is significantly enriched in exosomes, which carry a cargo of immunosuppressive molecules and deliver them to recipient immune cells, suppressing their functions. However, whether these exosomes origi...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2019.07.005

    authors： Hong CS,Danet-Desnoyers G,Shan X,Sharma P,Whiteside TL,Boyiadzis M

    更新日期：2019-08-01 00:00:00

  • Neonatal gene therapy. transfer and expression of exogenous genes in neonatal sheep following direct injection of retroviral vectors into the bone marrow space.

    abstract：:We investigated whether gene transfer into hematopoietic cells could be achieved by direct injection of retroviral vector supernatant into the bone marrow space of newborn sheep. Six sheep (5 weeks old) were injected bilaterally with either 1 mL of G1nBgSvNa8.1 vector supernatant (titer: 1 x 10(7)) in each hip (n = 5)...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(00)00158-2

    authors： Porada CD,Tran ND,Zhao Y,Anderson WF,Zanjani ED

    更新日期：2000-06-01 00:00:00

  • Targeting cell-bound MUC1 on myelomonocytic, monocytic leukemias and phenotypically defined leukemic stem cells with anti-SEA module antibodies.

    abstract：:Cell surface molecules aberrantly expressed or overexpressed by myeloid leukemic cells represent potential disease-specific therapeutic targets for antibodies. MUC1 is a polymorphic glycoprotein, the cleavage of which yields two unequal chains: a large extracellular α subunit containing a tandem repeat array bound in ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2018.12.002

    authors： Guillaume T,Dehame V,Chevallier P,Peterlin P,Garnier A,Grégoire M,Pichinuk E,Rubinstein DB,Wreschner DH

    更新日期：2019-02-01 00:00:00

  • Pluripotent stem cells with normal or reduced self renewal survive lethal irradiation.

    abstract：:Transfusion with 10,000 or 20,000 marrow cells resulted in 30+ days survival of 15%-50% of mice exposed to an Ld90 or LD100 or radiation. The use of congenic mice with alloenzyme markers permitted the identification of host and donor cells in the peripheral blood of transfused animals. Donor cells were present initial...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Brecher G,Neben S,Yee M,Bullis J,Cronkite EP

    更新日期：1988-08-01 00:00:00

  • Androgen receptor expression in mantle cell lymphoma: Potential novel therapeutic implications.

    abstract：:Mantle cell lymphoma (MCL) affects approximately 4500 patients/year in the US and demonstrates a male to female ratio of approximately 4:1. While the pathobiology underlying this ratio is unknown, the hematopoietic system is characterized by sex-related differences in androgen receptor (AR) expression, leading us to h...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2017.01.001

    authors： Mostaghel EA,Martin PS,Mongovin S,Frayo S,Zhang A,Edlefsen KL,Press OW,Gopal AK

    更新日期：2017-05-01 00:00:00

  • Mesenchymal stem cell-educated macrophages: a novel type of alternatively activated macrophages.

    abstract：OBJECTIVE:Mesenchymal stem cells (MSCs) are capable of modulating the immune system through interaction with a wide range of immune cells. This study investigates the hypothesis that interaction of MSCs with macrophages could play a significant role in their antiinflammatory/immune modulatory effects. MATERIALS AND ME...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2009.09.004

    authors： Kim J,Hematti P

    更新日期：2009-12-01 00:00:00

  • Loss of the tumor suppressor p15Ink4b enhances myeloid progenitor formation from common myeloid progenitors.

    abstract：OBJECTIVE:The tumor suppressor p15Ink4b (Ink4b) is a cell-cycle inhibitor that is inactivated in a high percentage of acute myeloid leukemia and myeloid dysplasia syndrome cases. Despite this, the role of Ink4b in hematopoiesis remains unclear. Here we examined the role of Ink4b in blood cell formation using Ink4b-defi...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2006.11.005

    authors： Rosu-Myles M,Taylor BJ,Wolff L

    更新日期：2007-03-01 00:00:00

  • Protection against fulminant sepsis in splenectomized mice by implantation of autochthonous splenic tissue.

    abstract：:Infection of splenectomized mice with Diplococcus pneumoniae, type III, resulted in a fourfold higher mortality rate than did infection of normal mice. Splenectomized animals were protected against fulminant, fatal sepsis by subcutaneous transplantation of autochthonous splenic tissue at the time of splenectomy. Anima...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Likhite VV

    更新日期：1978-05-01 00:00:00

  • A rare subgroup of leukemia stem cells harbors relapse-inducing potential in acute lymphoblastic leukemia.

    abstract：:After initially successful chemotherapy, relapse frequently jeopardizes the outcome of patients with acute leukemia. Because of their adverse characteristics of self-renewal and dormancy, leukemia stem cells have been hypothesized to play a critical role in resistance to antiproliferative chemotherapy and the developm...

    journal_title：Experimental hematology

    pub_type： 杂志文章,评审

    doi：10.1016/j.exphem.2018.09.006

    authors： Senft D,Jeremias I

    更新日期：2019-01-01 00:00:00

  • Human chronic myeloid leukemic cell line with positive Philadelphia chromosome exhibits megakaryocytic and erythroid characteristics.

    abstract：:A cell line (LAMA-84) has been established from the blood of a patient with chronic myeloid leukemia in acute phase. LAMA-84 cells retained the patient's chromosome abnormalities, i.e., triplication of all chromosomes except chromosome 18, the presence of Philadelphia (Ph) chromosome in 4-5 copies, and the presence of...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Seigneurin D,Champelovier P,Mouchiroud G,Berthier R,Leroux D,Prenant M,McGregor J,Starck J,Morle F,Micouin C

    更新日期：1987-09-01 00:00:00

  • Altered phenotype of natural killer cell subsets after haploidentical stem cell transplantation.

    abstract：OBJECTIVE:Haplotype-mismatched CD34(+) selected allogeneic stem cell transplantation (HASCT) has been described as a therapeutic option for patients with acute myeloid leukemia. The success of this regimen is based mainly on natural killer (NK) cell-mediated antileukemia effects. MATERIALS AND METHODS:We prospectively...

    journal_title：Experimental hematology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.exphem.2007.12.006

    authors： Schulze A,Schirutschke H,Oelschlägel U,Schmitz M,Füssel M,Wassmuth R,Ehninger G,Bornhäuser M,Platzbecker U

    更新日期：2008-04-01 00:00:00

  • Erythropoiesis in the X-irradiated, bled rat.

    abstract：:Exposure to low levels of x-irradiation (50R) followed by phlebotomy (50% blood volume) one month post-irradiation, resulted in identifiable alterations of the erythropoietic status of the rat. The red cell indices revealed a decrease in mean corpuscular volume and an increase in mean corpuscular hemoglobin concentrat...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Marchetti DL,Glomski CA

    更新日期：1975-11-01 00:00:00

  • Selectins and anti-CD15 (Lewis x/a) antibodies transmit activation signals in Hodgkin's lymphoma-derived cell lines.

    abstract：OBJECTIVE:The CD15 (Lewis x) cell surface oligosaccharide moiety is expressed in a variety of normal and tumor cells and recognized by selectins. The detection of CD15 on malignant Hodgkin-Reed-Sternberg (HRS) cells serves as a diagnostic marker of Hodgkin's lymphoma (HL). Retrospective studies suggest that the express...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/s0301-472x(03)00237-6

    authors： Ohana-Malka O,Benharroch D,Isakov N,Prinsloo I,Shubinsky G,Sacks M,Gopas J

    更新日期：2003-11-01 00:00:00

  • Bone marrow repopulation by human marrow stem cells after long-term expansion culture on a porcine endothelial cell line.

    abstract：:In vitro exposure of murine hematopoietic stem cells (HSCs) to cell cycle-inducing cytokines has been shown to result in a defect in the ability of these cells to engraft. We used a porcine microvascular endothelial cell (PMVEC) line in conjunction with exogenous interleukin (IL)-3, IL-6, granulocyte-macrophage colony...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Brandt JE,Galy AH,Luens KM,Travis M,Young J,Tong J,Chen S,Davis TA,Lee KP,Chen BP,Tushinski R,Hoffman R

    更新日期：1998-09-01 00:00:00

  • Quantitative assessment of human T lymphocytes in RAG2(-/-)gammac(-/-) mice: the impact of ex vivo manipulation on in vivo functionality.

    abstract：OBJECTIVE:Recent clinical trials of adoptive immunotherapy showed diminished reactivity of human T cells upon ex vivo manipulation. For a safe and effective clinical application of human T cells, it is necessary to improve ex vivo manipulation procedures and evaluate their impact on in vivo functionality. However, ther...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2006.09.018

    authors： van Rijn RS,Simonetti ER,Hagenbeek A,Bonyhadi M,Storm G,Martens AC,Ebeling SB

    更新日期：2007-01-01 00:00:00

  • Mobilization with granulocyte colony-stimulating factor blocks medullar erythropoiesis by depleting F4/80(+)VCAM1(+)CD169(+)ER-HR3(+)Ly6G(+) erythroid island macrophages in the mouse.

    abstract：:Similarly to other tissues, the bone marrow contains subsets of resident tissue macrophages, which are essential to maintain bone formation, functional hematopoietic stem cell (HSC) niches, and erythropoiesis. Pharmacologic doses of granulocyte colony-stimulating factor (G-CSF) mobilize HSC in part by interfering with...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2014.03.009

    authors： Jacobsen RN,Forristal CE,Raggatt LJ,Nowlan B,Barbier V,Kaur S,van Rooijen N,Winkler IG,Pettit AR,Levesque JP

    更新日期：2014-07-01 00:00:00

  • Sustained telomere erosion due to increased stem cell turnover during triple autologous hematopoietic stem cell transplantation.

    abstract：:Telomeres cap chromosomal ends and are shortened throughout a lifetime. Additional telomere erosion has been documented during conventional chemotherapy or hematopoietic stem cell transplantation. Previous studies of stem cell transplantation reported variable amounts of telomere shortening with inconsistent results r...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：10.1016/j.exphem.2007.08.028

    authors： Widmann T,Kneer H,König J,Herrmann M,Pfreundschuh M

    更新日期：2008-01-01 00:00:00

  • Direct synergistic effects of IL-4 and IL-11 on proliferation of primitive hematopoietic progenitor cells.

    abstract：:The present studies have investigated, for the first time, the synergistic effects of interleukin-4 (IL-4) and IL-11 on the growth of single murine bone marrow progenitor cells. These studies suggest that IL-4 and IL-11 are synergistic hematopoietic growth factors, enhancing colony formation of bone marrow progenitors...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Jacobsen FW,Keller JR,Ruscetti FW,Veiby OP,Jacobsen SE

    更新日期：1995-08-01 00:00:00

  • Fluctuations of BFUe and CFUe cycling after erytrhoid perturbations: correlation with variations of pool size.

    abstract：:The pool size of erythroid burst-(BFUe) and colony-forming units (CFUe) has been evaluated in normal or hypoxia-induced polycythemic mice at sequential times after either transfusion or administration of purified erythropoietin (Ep). The present investigations were focused on the in vitro tritiated thymidine (3H-TdR) ...

    journal_title：Experimental hematology

    pub_type： 杂志文章

    doi：

    authors： Peschle C,Cillo C,Migliaccio G,Lettieri F

    更新日期：1980-01-01 00:00:00