Abstract:
:The Polymerase Chain Reaction (PCR) is a revolutionary new means of amplifying, i.e., replicating, selected DNA sequences in vitro. This procedure is highly sensitive and rapid, requiring few hours for detection of amplified target DNA whereas it often takes several weeks for the identification of many fastidious organisms by culture. Studies have shown that as few as one microorganism can be detected by PCR. Therefore, it is revolutionizing our ability in the diagnosis of infectious diseases. Currently, this technique is very expensive and complex which will probably restrict its immediate role for diagnosing infections where the causative organism is impossible to culture or untypeable by conventional means. As further progress occurs, however, this powerful technique will be applied as a major new tool to diagnose infections and to the study of individual genome variations.
journal_name
Indian J Pediatrjournal_title
Indian journal of pediatricsauthors
Raj Pdoi
10.1007/BF02737744keywords:
subject
Has Abstractpub_date
1997-07-01 00:00:00pages
435-40issue
4eissn
0019-5456issn
0973-7693journal_volume
64pub_type
杂志文章,评审abstract:OBJECTIVE:To determine the correlation between various obesity indices and pulmonary function parameters in obese Thai children and adolescents. METHODS:Obese children and adolescents aged from 8 to 18 y and diagnosed under the criteria of International Obesity Task Force (IOTF) were enrolled. Anthropometric and body ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-015-1777-4
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:To develop a height and weight based equation to estimate total body water (TBW) in Sri Lankan children. METHODS:Cross sectional descriptive study done involving 5-15 year old healthy children. Height and weight were measured. TBW was assessed using isotope dilution method (D(2)O) and fat free mass (FFM) cal...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-009-0333-5
更新日期:2010-02-01 00:00:00
abstract::Children who had undergone adenotonsillectomy for recurrent adenotonsillitis showing no signs of clinical or radiological obstructive manifestations were evaluated with pulmonary function tests before, and one month after the operation. In relation to the result obtained by function tests, 60% of 45 cases (27) had the...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02822185
更新日期:1993-03-01 00:00:00
abstract:OBJECTIVE:To identify the causes and contributing factors of persistent pneumonia in children. METHODS:41 cases with persistent pneumonia were investigated (biochemical, microbiological, histopathological, immunological and radiological tests) to find out the underlying cause. RESULTS:Out of 41 cases, 8 had pulmonary...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-009-0272-1
更新日期:2009-12-01 00:00:00
abstract:: ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-018-2720-2
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:To investigate correlations between anthropometrics and body composition with bone parameters of the whole-body and lumbar spine in non-obese and obese Thai female adolescents. METHODS:This study was performed in 135 female adolescents aged 15 to 18 y enrolled in secondary schools in southern Thailand. Subje...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-017-2422-1
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVE:To evaluate clinical, etiological and EEG profile of neonatal seizures. METHODS:In all the neonates enrolled in the study baseline information recorded was sex, gestational age, weight, ponderal index etc. Clinical profile of seizure episode included age at onset of seizure, type and duration of seizure, ass...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-007-0023-0
更新日期:2007-01-01 00:00:00
abstract::Auditory evoked responses using BERA were studied in 30 newborn babies with plasma bilirubin > or = 15 mgm/dl and repeated after treatment of neonatal jaundice with bilirubin levels of < or = 10 mgm/dl. A few jaundiced babies (16.5%) showed absent BERA response at the initial/subsequent examination. After treatment, 3...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02751203
更新日期:1993-05-01 00:00:00
abstract:OBJECTIVE:To describe the needs of relatives of children admitted to an Intensive Care Unit and compare their needs with the perspectives of doctors, nurses and administrators. METHODS:This is a descriptive comparative study done at a tertiary care PICU from South India. A modified Critical Care Family Needs Inventory...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-017-2324-2
更新日期:2017-08-01 00:00:00
abstract::Naxos disease (OMIM 601214) characterized by wooly hair, palmoplantar keratoderma and dilated cardiomyopathy in two Indian children of the same family is described. ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02825834
更新日期:2006-04-01 00:00:00
abstract:OBJECTIVES:In this pilot study, the authors developed and evaluated a working memory intervention (WMI) using a combination of mobile phone-based application and an activity booklet, among children with idiopathic generalized epilepsy. METHODS:Pre- and post-intervention cognitive evaluation at 8 wk included: subtests ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-020-03653-w
更新日期:2021-01-09 00:00:00
abstract:OBJECTIVE:To identify the risk factors for small for gestational age (SGA) babies. METHODS:The present study was a nested case control study which was carried out by two Medical Officers of Health areas (The field level administrative unit responsible for provision of preventive and promotive health services) in Colom...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-014-1382-y
更新日期:2014-10-01 00:00:00
abstract::Hitherto poor outcomes, paucity of data and heterogeneity in International approach to Pediatric NHL (Non-Hodgkin Lymphoma) prompted the need for guidelines for Indian population with vast variability in access, affordability and infrastructure across the country. These guidelines are based on consensus among the expe...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章,评审
doi:10.1007/s12098-017-2318-0
更新日期:2017-05-01 00:00:00
abstract::Incontinence disorders are an important group of problems that clinicians manage in children and adolescents. This paper reviews the physiology of micturition, the epidemiology of enuresis, etiologic concepts of incontinence disorders, a general clinical approach to enuretic patients, laboratory evaluation and general...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章,评审
doi:10.1007/BF02845537
更新日期:1999-05-01 00:00:00
abstract::A comparison of mortality and morbidity pattern of hospital admissions of children under 14 years during 1966-68 and 1977-81 has been made. Annual admission rate has increased from 1515 to 2515, which is in proportion to the population increase of 3 lacs from 1966 to 1981. Recently more than 70% were discharged within...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02726772
更新日期:1990-07-01 00:00:00
abstract::Epilepsy is a common childhood neurological morbidity needs careful evaluation, relevant investigations and precise therapy with appropriate antiepileptic drugs for optimal duration. Majority of childhood epilepsy remits with antiepileptic drugs and should be managed in the community. Practising pediatricians must cou...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02723743
更新日期:2003-02-01 00:00:00
abstract::Patients infected with the human immunodeficiency virus (HIV) commonly experience diarrhea at some time during their illness. A variety of enteric pathogens are identified in 50-80% of these patients, depending on the intensity of the diagnostic work-up that is done. In addition to the common enteric pathogens, severa...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章,评审
doi:10.1007/BF02752359
更新日期:1999-01-01 00:00:00
abstract:OBJECTIVES:To study whether orogastric tube (OGT) insertion elicits a painful response in preterm neonates, and the role of oral sucrose in reducing this pain. METHODS:This double blinded, randomized control trial was conducted in the neonatal intensive care units of Kalawati Saran Children's Hospital. Clinically stab...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12098-012-0924-4
更新日期:2013-06-01 00:00:00
abstract::A 11-year-old girl presented with thrombocytopenic purpura along with cervical lymphadenopathy. There was no hepatosplenomegaly; no evidence of microangiopathy; bone marrow examination was normal. Acid fast bacilli were seen in fine needle aspiration cytology of the lymph node. A diagnosis of tubercular lymphadenitis ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02723827
更新日期:2003-12-01 00:00:00
abstract::Retroperitoneum, pelvis and scrotum are unusual sites of lymphangiomas. Retroperitoneal lymphangiomas presenting as inguino-scrotal masses are extremely rare. An unusual case of retroperitoneal lymphangioma extending into scrotum, presenting as acute scrotum is reported due to its unusual location. Complete surgical e...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02726134
更新日期:1997-09-01 00:00:00
abstract:OBJECTIVES:To screen for variants in the MC4R and LEP genes in 46 patients with clinical suspicion of non-syndromic early onset severe obesity (NEOSO). METHODS:Children with early onset obesity satisfying WHO criteria of obesity were studied. The MC4R and LEP genes were sequenced using a PCR amplicon based NGS on Illu...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-019-03129-6
更新日期:2020-02-01 00:00:00
abstract::Upper gastrointestinal bleeding is a life threatening condition in children. Common sources of upper gastrointestinal bleeding in children include variceal hemorrhage (most commonly extra-hepatic portal venous obstruction in our settings) and mucosal lesions (gastric erosions and ulcers secondary to drug intake). Whil...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-010-0296-6
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:This study was planned to evaluate the MCH services, particularly immunization in rural areas of the poor-performing state of Rajasthan. METHODS:A community-based, cross-sectional survey using the WHO 30 cluster technique was carried out as a field exercise by participants of 9th Field Epidemiology Training ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02758259
更新日期:2006-01-01 00:00:00
abstract::We present a preterm neonate who developed congenital rubella syndrome in a mother who had rubella at 5 months of gestation. An amniocentesis was done in the mother at that time but amniotic fluid rubella PCR was negative. Thus, inspite of prenatal screening, one cannot definitely conclude absence of perinatal transmi...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/s12098-009-0312-x
更新日期:2010-04-01 00:00:00
abstract::Advances in diagnosis and treatment along with improved supportive care have contributed to the current survival rates for pediatric malignancies. Recent concept of a truly "cured child" in pediatric oncology envisages not only a biological cure of the disease but a child on par with peers in growth and development ph...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02723807
更新日期:2003-10-01 00:00:00
abstract::This study was designed to compare two methods of breast milk expression, namely, the manual and the pumping method using a hand-held cylindric pump. The parameters evaluated were (i) the output of breast milk during milk expression sessions of 15 minutes' duration, and, (ii) the subjective preference of the method by...
journal_title:Indian journal of pediatrics
pub_type: 临床试验,杂志文章
doi:10.1007/BF02823873
更新日期:1996-01-01 00:00:00
abstract::A young girl with an uncommon renal tumour is the subject of this communication. A diagnosis of renal cell carcinoma was established post-operatively. Combined modality treatment including chemotherapy and radiotherapy has helped achieve a disease free survival of nearly 5 years. ...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02761220
更新日期:1999-03-01 00:00:00
abstract::Medical bioethics has developed as a new field of study in the US in the past quarter decade, focusing upon the moral principles of autonomy, beneficence, nonmaleficence, and justice. These principles may, however, reflect biases in American culture, rather than a truly universal approach to ethical problems in medici...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章,评审
doi:10.1007/BF02845196
更新日期:1997-05-01 00:00:00
abstract:OBJECTIVE:This study was carried out to evaluate the adequacy of seroconversion when Hepatitis B vaccine is given along with other vaccines at 0, 6 weeks (along with DPT and OPV) and at 9 months (along with measles). METHODS:725 infants born to apparently healthy mothers were enrolled to receive the hepatitis B vaccin...
journal_title:Indian journal of pediatrics
pub_type: 临床试验,杂志文章
doi:10.1007/BF02724073
更新日期:2005-08-01 00:00:00
abstract::Fifteen low birth weight premature infants with a diagnosis of PDA were administered 0·2 mg/kg of indomethacin orally, 3 doses at 12 hourly intervals. The results were compared with 18 prematures who were managed by fluid restriction (80-100 ml/kg/day) and decongestive therapy. Nine out of the 15 cases who received in...
journal_title:Indian journal of pediatrics
pub_type: 杂志文章
doi:10.1007/BF02749534
更新日期:1986-07-01 00:00:00