Markers of cell proliferation and expression of melanosomal antigen in lymphangioleiomyomatosis.

abstract：:Morphologic changes are reported to occur in rat lung vasculature after 3 days of hypoxia. We have previously shown that immunoreactivity for the vasodilator calcitonin gene-related peptide (CGRP) is increased in pulmonary endocrine cells by 7 days of hypoxia. Because these cells may be among the earliest mediators of...

journal_title：American journal of respiratory cell and molecular biology

authors： Roncalli M,Springall DR,Maggioni M,Moradoghli-Haftvani A,Winter RJ,Zhao L,Coggi G,Polak JM

更新日期：1993-11-01 00:00:00

abstract：:Mechanical ventilation with high tidal volumes has been associated with pulmonary alveolar flooding. Understanding the mechanisms underlying cyclic stretch-induced increases in alveolar epithelial permeability may be important in designing preventive measures for acute lung injury. In this work, we assessed whether cy...

journal_title：American journal of respiratory cell and molecular biology

authors： Davidovich N,DiPaolo BC,Lawrence GG,Chhour P,Yehya N,Margulies SS

更新日期：2013-07-01 00:00:00

abstract：:Organisms on our planet have evolved in an oxidizing environment that is intrinsically inimical to life, and cells have been forced to devise means of protecting themselves. One of the defenses used most widely in nature is the enzyme heme oxygenase-1 (HO-1). This enzyme performs the seemingly lackluster function of c...

journal_title：American journal of respiratory cell and molecular biology

authors： Morse D,Choi AM

更新日期：2002-07-01 00:00:00

abstract：:We have tested the murine macrophagic cell line RAW 264.7 for its ability to undergo activation after exposure to silica particles in vitro. When exposed to silica under controlled conditions (each cell having access to about 10 silica particles), RAW 264.7 cells were able to phagocytose the particles. Concomitantly, ...

journal_title：American journal of respiratory cell and molecular biology

authors： Claudio E,Segade F,Wróbel K,Ramos S,Lazo PS

更新日期：1995-11-01 00:00:00

abstract：:The hydroxyl radical (.OH) is a highly reactive oxygen free radical that has been implicated as a cause of lung injury following exposure to silica and silicates. Despite evidence that silica generates .OH in vitro, there has been no previous demonstration of in vivo production of .OH after exposure to nonfibrous mine...

journal_title：American journal of respiratory cell and molecular biology

authors： Schapira RM,Ghio AJ,Effros RM,Morrisey J,Almagro UA,Dawson CA,Hacker AD

更新日期：1995-02-01 00:00:00

abstract：:Cigarette smoke (CS) is the main causative factor of chronic obstructive pulmonary disease (COPD). Current research supports the concept that airway inflammation is central to the development and progression of the disease. Studies have demonstrated that neutrophils are increased in COPD lungs and that neutrophil-asso...

journal_title：American journal of respiratory cell and molecular biology

authors： Cardini S,Dalli J,Fineschi S,Perretti M,Lungarella G,Lucattelli M

更新日期：2012-09-01 00:00:00

abstract：:To investigate mechanisms regulating intra-alveolar coagulation, we studied monolayers of the A549 human lung epithelial cell line. The surface of A549 cells delayed the onset of prothrombin-to-thrombin conversion and prevented total prothrombin consumption in normal plasma compared to plastic cell-free wells. Similar...

journal_title：American journal of respiratory cell and molecular biology

authors： Berry L,Andrew M,Post M,Ofosu F,O'Brodovich H

更新日期：1991-04-01 00:00:00

abstract：:An NADPH-oxidase complex containing at least two protein components (gp91-phox and p22-phox) and a unique low redox potential (-245 mV) cytochrome b-245 is the source of superoxide generated for bacterial killing in neutrophils and has been suggested as the oxygen sensor in the carotid body. In pure cultures of smooth...

journal_title：American journal of respiratory cell and molecular biology

authors： Marshall C,Mamary AJ,Verhoeven AJ,Marshall BE

更新日期：1996-11-01 00:00:00

abstract：:It is known that interleukin (IL)-23, an IL-12-family cytokine, can be released by certain antigen-presenting cells in response to bacterial pathogens. Recent in vitro studies indicate that this cytokine stimulates a unique subset of CD4 cells, the T helper cell (Th)17 subset, to produce and release the proinflammator...

journal_title：American journal of respiratory cell and molecular biology

authors： Ivanov S,Bozinovski S,Bossios A,Valadi H,Vlahos R,Malmhäll C,Sjöstrand M,Kolls JK,Anderson GP,Lindén A

更新日期：2007-04-01 00:00:00

abstract：:Excessive growth of pulmonary arterial (PA) smooth muscle cells (SMCs) is a major component of PA hypertension (PAH). The calcium-activated neutral cysteine proteases calpains 1 and 2, expressed by PASMCs, contribute to PH but are tightly controlled by a single specific inhibitor, calpastatin. Our objective was to inv...

journal_title：American journal of respiratory cell and molecular biology

authors： Wan F,Letavernier E,Abid S,Houssaini A,Czibik G,Marcos E,Rideau D,Parpaleix A,Lipskaia L,Amsellem V,Gellen B,Sawaki D,Derumeaux G,Dubois-Randé JL,Delcroix M,Quarck R,Baud L,Adnot S

更新日期：2016-09-01 00:00:00

abstract：:We investigated the effects of cultured epithelial cells and supernatants on resting membrane potential and excitatory neuroeffector transmission in smooth muscle cells of dog trachea and bronchioles. The mean resting membrane potential of the mucosa-free tracheal smooth muscle cells was -59.5 +/- 1.5 mV (+/- SD). App...

journal_title：American journal of respiratory cell and molecular biology

authors： Matsumoto K,Aizawa H,Inoue R,Hamano S,Ikeda S,Xie Z,Hirata M,Hara N,Ito Y

更新日期：1994-03-01 00:00:00

abstract：:Identification of the genetic loci underlying asthma in humans has been hampered by variability in clinical phenotype, uncontrolled environmental influences, and genetic heterogeneity. To circumvent these complications, the genetic regulation of asthma-associated phenotypes was studied in a murine model. We characteri...

journal_title：American journal of respiratory cell and molecular biology

authors： Ewart SL,Kuperman D,Schadt E,Tankersley C,Grupe A,Shubitowski DM,Peltz G,Wills-Karp M

更新日期：2000-10-01 00:00:00

abstract：:Bleomycin-induced lung disease is characterized by cell injury followed by fibroblast proliferation. Cells respond to injury by synthesizing a family of heat shock proteins. These proteins are critical to cell survival, and those of the 70,000 MW group (hsp 70) are essential for cell division and proliferation. To eva...

journal_title：American journal of respiratory cell and molecular biology

authors： Moseley PL,York SJ,York J

更新日期：1989-08-01 00:00:00

abstract：:Laminins are heterotrimeric proteins that are secreted by the alveolar epithelium into the basement membrane, and their expression is altered in extracellular matrices from patients with pulmonary fibrosis. In a small number of patients with pulmonary fibrosis, we found that the normal basement membrane distribution o...

journal_title：American journal of respiratory cell and molecular biology

authors： Morales-Nebreda LI,Rogel MR,Eisenberg JL,Hamill KJ,Soberanes S,Nigdelioglu R,Chi M,Cho T,Radigan KA,Ridge KM,Misharin AV,Woychek A,Hopkinson S,Perlman H,Mutlu GM,Pardo A,Selman M,Jones JC,Budinger GR

更新日期：2015-04-01 00:00:00

abstract：:The dynamics and mechanisms of extracellular release of hydrogen peroxide (H2O2) from bovine pulmonary artery endothelial cells (EC) subjected to anoxia, hypoxia, and hypoxia followed by reoxygenation were examined using various inhibitors of enzymatic systems in intact cells and by direct measurement of H2O2 producti...

journal_title：American journal of respiratory cell and molecular biology

authors： Zulueta JJ,Yu FS,Hertig IA,Thannickal VJ,Hassoun PM

更新日期：1995-01-01 00:00:00

abstract：:Mycobacterium avium (MAV) and M. abscessus (MAB) are ubiquitous environmental organisms increasingly recognized to cause chronic lung disease in patients with apparently normal immune function. Little is yet known about their human pathophysiology. Our objective was to examine cytokine and chemokine responses (protein...

journal_title：American journal of respiratory cell and molecular biology

authors： Sampaio EP,Elloumi HZ,Zelazny A,Ding L,Paulson ML,Sher A,Bafica AL,Shea YR,Holland SM

更新日期：2008-10-01 00:00:00

abstract：:Asthma is characterized by appearance of eosinophils in the airway. Eosinophils purified from the airway 48 h after segmental antigen challenge are described as exhibiting greater adhesion to albumin-coated surfaces via an unidentified beta2 integrin and increased expression of alphaMbeta2 (CD11b/18) compared with pur...

journal_title：American journal of respiratory cell and molecular biology

authors： Barthel SR,Jarjour NN,Mosher DF,Johansson MW

更新日期：2006-09-01 00:00:00

abstract：:Chronic hypoxia induces proliferation of human pulmonary artery smooth muscle cells (hPASMCs), leading to remodeling and pulmonary hypertension, but the mechanism remains unclear. The present study tested the roles of mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) and mitochondrial membrane potential (Delt...

journal_title：American journal of respiratory cell and molecular biology

authors： Hu HL,Zhang ZX,Chen CS,Cai C,Zhao JP,Wang X

更新日期：2010-06-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF-alpha) is thought to be important in the development of pulmonary fibrosis. However, surfactant protein-C/TNF-alpha transgenic mice do not spontaneously develop pulmonary fibrosis but instead develop alveolar enlargement and loss of elastic recoil. We hypothesized that overexpression o...

journal_title：American journal of respiratory cell and molecular biology

authors： Fujita M,Shannon JM,Morikawa O,Gauldie J,Hara N,Mason RJ

更新日期：2003-12-01 00:00:00

abstract：:The reasons for bacterial proliferation in the lungs of mechanically ventilated patients are poorly understood. We hypothesized that prolonged cyclic stretch of lung cells influenced bacterial growth. Human alveolar type II-like A549 cells were submitted in vitro to prolonged cyclic stretch. Bacteria were cultured in ...

journal_title：American journal of respiratory cell and molecular biology

authors： Pugin J,Dunn-Siegrist I,Dufour J,Tissières P,Charles PE,Comte R

更新日期：2008-03-01 00:00:00

abstract：:Stimulated migration of eosinophils out of the bloodstream and into the lung is key in the development of tissue eosinophilia and inflammation in asthma. Platelet-activating factor (PAF) has been implicated as an important inflammatory mediator in asthma pathogenesis in part because of its chemotactic capacity. We the...

journal_title：American journal of respiratory cell and molecular biology

authors： Casale TB,Erger RA,Little MM

更新日期：1993-01-01 00:00:00

abstract：:In surgically excised nasal polyps, most epithelial mast cells were formalin sensitive, chloroacetate esterase (CAE) negative, and chymase negative. Thus, this represents a population of mast cells not identified by staining for CAE. On the other hand, most stromal mast cells were formalin resistant and CAE positive, ...

journal_title：American journal of respiratory cell and molecular biology

authors： Kawabori S,Denburg JA,Schwartz LB,Irani AA,Wong D,Jordana G,Evans S,Dolovich J

更新日期：1992-01-01 00:00:00

abstract：:Alveolar macrophages (AM) express gelatinase B, a member of the matrix metalloproteinase family involved in the degradation and remodeling of extracellular matrix components. We evaluated the expression of gelatinase B in the course of idiopathic pulmonary fibrosis (IPF) by studying alveolar macrophages in culture AM ...

journal_title：American journal of respiratory cell and molecular biology

authors： Lemjabbar H,Gosset P,Lechapt-Zalcman E,Franco-Montoya ML,Wallaert B,Harf A,Lafuma C

更新日期：1999-05-01 00:00:00

abstract：:The lung is richly supplied with peptidergic nerves that store and secrete substance P (SP), vasoactive intestinal peptide (VIP), and other neuropeptides known to potently modulate leukocyte function in vitro and airway inflammation in vivo. To investigate and characterize neuromodulation of immune responses compartme...

journal_title：American journal of respiratory cell and molecular biology

authors： Kaltreider HB,Ichikawa S,Byrd PK,Ingram DA,Kishiyama JL,Sreedharan SP,Warnock ML,Beck JM,Goetzl EJ

更新日期：1997-02-01 00:00:00

abstract：:Lung fibrosis has been postulated to be mediated by the production of macrophage-derived growth factors that are both mitogenic and chemotactic for fibroblasts. In vitro studies from our laboratory demonstrated that alveolar and interstitial macrophages treated with iron and asbestos release platelet-derived growth fa...

journal_title：American journal of respiratory cell and molecular biology

authors： Osornio-Vargas AR,Kalter VG,Badgett A,Hernández-Rodríguez N,Aguilar-Delfín I,Brody AR

更新日期：1993-05-01 00:00:00

abstract：:IL-13 is a T-helper class 2 cytokine that induces goblet cell hyperplasia and mucus production in airway epithelial cells. Because macrolide antibiotics are known to have immunomodulatory and mucoregulatory properties, the aim of this study was to examine the effect of clarithromycin on IL-13-induced goblet cell hyper...

journal_title：American journal of respiratory cell and molecular biology

authors： Tanabe T,Kanoh S,Tsushima K,Yamazaki Y,Kubo K,Rubin BK

更新日期：2011-11-01 00:00:00

abstract：:The effects of concomitant P1-receptor stimulation on peak intracellular Ca2+ release by extracellular adenosine 5'-triphosphate (ATP) and 5-hydroxytryptamine (5-HT) were investigated in cultured airway smooth-muscle (ASM) cells. The results show that peak Ca2+ release to ATP is enhanced by preincubation with adenosin...

journal_title：American journal of respiratory cell and molecular biology

authors： Michoud MC,Tao FC,Pradhan AA,Martin JG

更新日期：1999-07-01 00:00:00

abstract：:Opiate-like peptides can regulate many cellular functions. We now map [D-Ala(2)]deltorphin I (DADTI)-like immunoreactivity (DADTI-LI) in developing mouse lung and analyze potential functional roles. Most DADTI-LI-positive cells were alveolar cells negative for prosurfactant protein (proSP)-C immunoreactivity. Peak num...

journal_title：American journal of respiratory cell and molecular biology

authors： Sunday ME,Haley KJ,Emanuel RL,Torday JS,Asokananthan N,Sikorski KA,Tooyama I,Kimura H,Renda T,Erspamer V

更新日期：2001-10-01 00:00:00

abstract：:Electrical field stimulation (EFS) has previously been shown to induce the release of prostaglandin (PG) E2 from ferret tracheal epithelium. We have now conducted a study to see whether this effect of EFS is due to the activation of nerves or whether it is a non-neural effect. The release of PGE2 and 6-keto-PGF1 alpha...

journal_title：American journal of respiratory cell and molecular biology

authors： Ullman A,Ciabattoni G,Svedmyr N,Skoogh BE,Löfdahl CG

更新日期：1991-03-01 00:00:00

abstract：:Hyperplasia of airway smooth muscle cells is present in the airways of asthmatic patients and may contribute to the development of the bronchial hyperresponsiveness that occurs in these patients. Because tryptase is an abundant component of mast cell granules and has demonstrated growth-stimulatory effects in other me...

journal_title：American journal of respiratory cell and molecular biology

authors： Brown JK,Tyler CL,Jones CA,Ruoss SJ,Hartmann T,Caughey GH

更新日期：1995-08-01 00:00:00