Early-passage rat lung fibroblasts do not migrate in vitro to transforming growth factor-beta.

abstract：:Infants born to heroin- and cocaine-addicted mothers have been reported to have a lower incidence of respiratory distress syndrome (RDS) compared with nonaddicted infants. However, it is not known whether these are direct drug-mediated effects or secondary phenomena. We therefore investigated the effect of opioids and...

journal_title：American journal of respiratory cell and molecular biology

authors： Gewolb IH,O'Brien J,Slavin RE

更新日期：1999-03-01 00:00:00

abstract：:β-Arrestin-2 (βarr2) is a ubiquitously expressed cytosolic protein that terminates G protein-coupled receptor signaling and transduces G protein-independent signaling. We previously showed that mice lacking βarr2 do not develop an asthma phenotype when sensitized to, and challenged with, allergens. The current study e...

journal_title：American journal of respiratory cell and molecular biology

authors： Chen M,Hegde A,Choi YH,Theriot BS,Premont RT,Chen W,Walker JK

更新日期：2015-09-01 00:00:00

abstract：:Tissue inhibitors of metalloproteinases (TIMPs) are multifunctional proteins that have the capacity to modify cellular activities and to modulate matrix turnover. We demonstrate that TIMP-1 messenger RNA (mRNA) and protein expression are selectively and markedly increased in a murine model of bleomycin-induced pulmona...

journal_title：American journal of respiratory cell and molecular biology

authors： Madtes DK,Elston AL,Kaback LA,Clark JG

更新日期：2001-05-01 00:00:00

abstract：:The doxycycline-inducible, gene regulatory system allows tight control of transgene expression for the study of organ development and disease pathogenesis. Multiple recent reports have employed this model to investigate various lung diseases including emphysema. For our study, we used this transgenic system to test wh...

journal_title：American journal of respiratory cell and molecular biology

authors： Sisson TH,Hansen JM,Shah M,Hanson KE,Du M,Ling T,Simon RH,Christensen PJ

更新日期：2006-05-01 00:00:00

abstract：:Platelet-activating factor (PAF) is a potent contractile agonist in guinea pig peripheral lung strips. Whether PAF acts via specific receptor sites in the lung has not been fully established. To determine if specific receptor sites could be demonstrated in guinea pig peripheral lung tissue, we performed direct radioli...

journal_title：American journal of respiratory cell and molecular biology

authors： Gomez J,Bloom JW,Yamamura HI,Halonen M

更新日期：1990-09-01 00:00:00

abstract：:Vascular remodeling is an important pathological feature of pulmonary arterial hypertension (PAH), which leads to increased pulmonary vascular resistance, with marked proliferation of pulmonary artery smooth muscle cells (SMC) and/or endothelial cells (EC). Successful treatment of experimental PAH with a platelet-deri...

journal_title：American journal of respiratory cell and molecular biology

authors： Sakao S,Tatsumi K,Voelkel NF

更新日期：2010-12-01 00:00:00

abstract：:The hydroxyl radical (.OH) is a highly reactive oxygen free radical that has been implicated as a cause of lung injury following exposure to silica and silicates. Despite evidence that silica generates .OH in vitro, there has been no previous demonstration of in vivo production of .OH after exposure to nonfibrous mine...

journal_title：American journal of respiratory cell and molecular biology

authors： Schapira RM,Ghio AJ,Effros RM,Morrisey J,Almagro UA,Dawson CA,Hacker AD

更新日期：1995-02-01 00:00:00

abstract：:The ability of Pseudomonas aeruginosa to form antibiotic-resistant biofilms is thought to account for the inability of current therapies to resolve bacterial infections in the lungs of patients with cystic fibrosis (CF). We recently described a system in which highly antibiotic-resistant P. aeruginosa biofilms grow on...

journal_title：American journal of respiratory cell and molecular biology

authors： Moreau-Marquis S,O'Toole GA,Stanton BA

更新日期：2009-09-01 00:00:00

abstract：:The cellular events involved in generating the hypoxic pulmonary vasoconstriction response are not clearly understood, in part because of the multitude of factors that alter pulmonary vascular tone. The goal of the present studies was to determine if a cell culture preparation containing vascular smooth muscle (VSM) c...

journal_title：American journal of respiratory cell and molecular biology

authors： Murray TR,Chen L,Marshall BE,Macarak EJ

更新日期：1990-11-01 00:00:00

abstract：:Airway surface fluids are mainly secreted from submucosal glands, and play important roles in the defense of airways via the up-regulation of mucociliary transport, resulting in an exclusion of many microbes or foreign substances. Although there are many articles concerning the importance of Toll-like receptors (TLRs)...

journal_title：American journal of respiratory cell and molecular biology

authors： Murakami K,Tamada T,Nara M,Muramatsu S,Kikuchi T,Kanehira M,Maruyama Y,Ebina M,Nukiwa T

更新日期：2011-11-01 00:00:00

abstract：:Asthma is characterized by appearance of eosinophils in the airway. Eosinophils purified from the airway 48 h after segmental antigen challenge are described as exhibiting greater adhesion to albumin-coated surfaces via an unidentified beta2 integrin and increased expression of alphaMbeta2 (CD11b/18) compared with pur...

journal_title：American journal of respiratory cell and molecular biology

authors： Barthel SR,Jarjour NN,Mosher DF,Johansson MW

更新日期：2006-09-01 00:00:00

abstract：:Hexavalent chromium (Cr[VI]) is classified by the International Agency for Research on Cancer as a group I carcinogen. Although the U.S. Occupational Safety and Health Administration was obliged to reduce the permissible exposure limit (PEL), it was reported that U.S. workers continue to be exposed to dangerously high...

journal_title：American journal of respiratory cell and molecular biology

authors： Russo P,Catassi A,Cesario A,Imperatori A,Rotolo N,Fini M,Granone P,Dominioni L

更新日期：2005-12-01 00:00:00

abstract：:Prior studies have demonstrated that human alveolar macrophages (AM) are suppressive of lymphocyte function, through the mechanism of inhibition is unclear. In the current study, human AM inhibited receptor-mediated increases in intracellular calcium concentration ([Ca2+]i) in T cells, natural killer cells, and B cell...

journal_title：American journal of respiratory cell and molecular biology

authors： Yarbrough WC Jr,Wilkes DS,Weissler JC

更新日期：1991-11-01 00:00:00

abstract：:Endogenous hydrogen peroxide (H2O2) release from aortic endothelial cells was studied in the presence of antioxidant enzyme inhibitors, mitochondrial inhibitors, a microsomal cytochrome P-450 inhibitor, and after oxidative stress induced with H2O2 or menadione. Extracellular H2O2 generation was determined spectrofluor...

journal_title：American journal of respiratory cell and molecular biology

authors： Kinnula VL,Whorton AR,Chang LY,Crapo JD

更新日期：1992-02-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a potent peptide growth factor specific for vascular endothelial cells, which promotes neovascularization and increases vascular permeability in vivo. Enhanced microvascular permeability and edema are common characteristics of inflammatory and neoplastic disorders. Two proi...

journal_title：American journal of respiratory cell and molecular biology

authors： Nauck M,Roth M,Tamm M,Eickelberg O,Wieland H,Stulz P,Perruchoud AP

更新日期：1997-04-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF), interleukin-1 beta (IL-1), interleukin-6 (IL-6), and interleukin-8 (IL-8) are inflammatory cytokines produced by alveolar macrophages (AMs) and implicated in sepsis-related adult respiratory distress syndrome (ARDS). Preliminary findings from clinical trials suggest that aerosolized ...

journal_title：American journal of respiratory cell and molecular biology

authors： Thomassen MJ,Meeker DP,Antal JM,Connors MJ,Wiedemann HP

更新日期：1992-09-01 00:00:00

abstract：:Previous studies have shown that the pan CD28/cytotoxic T lymphocyte antigen (CTL)A-4 antagonist CTLA4 immunoglobulin (Ig) inhibits eosinophilic airway inflammation in Schistosoma mansoni-sensitized and airway-challenged mice. In the present study, the importance of CD28 as well as the individual roles of CD80 and CD8...

journal_title：American journal of respiratory cell and molecular biology

authors： Mathur M,Herrmann K,Qin Y,Gulmen F,Li X,Krimins R,Weinstock J,Elliott D,Bluestone JA,Padrid P

更新日期：1999-10-01 00:00:00

abstract：:Mucin glycoproteins play a key role in the normal function of the airway epithelium. We examined the expression of mucin genes, MUC3, 4, 5AC, 5B, 6, 7, and 8 in human fetal tissues to establish the localization and age of onset of expression of each mucin gene during human development. We detected expression of MUC4, ...

journal_title：American journal of respiratory cell and molecular biology

authors： Reid CJ,Gould S,Harris A

更新日期：1997-11-01 00:00:00

abstract：:Pulmonary carbonic anhydrase (CA) activity plays important roles in carbon dioxide exchange, fluid secretion, and pH regulation. This study reports the use of molecular and immunologic techniques to characterize expression of the high-activity cytosolic isoenzyme CA II in rat lung tissue. Northern blot analysis of RNA...

journal_title：American journal of respiratory cell and molecular biology

authors： Fleming RE,Moxley MA,Waheed A,Crouch EC,Sly WS,Longmore WJ

更新日期：1994-05-01 00:00:00

abstract：:Idiopathic pulmonary fibrosis (IPF) is a chronic fibrosing lung disease of indeterminate etiology and limited therapeutic options. The initiation, development, and progression of IPF are influenced by genetic predisposition, aging, and host and environmental factors, but the magnitude of the contribution of each of th...

journal_title：American journal of respiratory cell and molecular biology

authors： Pardo A,Selman M

更新日期：2020-09-18 00:00:00

abstract：:The lung epithelium has recently been identified as a novel site of fibrinogen (FBG) biosynthesis. A coordinated upregulation of A alpha, B beta, and gamma chain FBG gene transcription occurs upon stimulation of A549 lung epithelial cells with dexamethasone (DEX) and the proinflammatory mediator interleukin-6 (IL-6). ...

journal_title：American journal of respiratory cell and molecular biology

authors： Guadiz G,Sporn LA,Goss RA,Lawrence SO,Marder VJ,Simpson-Haidaris PJ

更新日期：1997-07-01 00:00:00

abstract：:We hypothesized that, as in animal models, localized deposition of antigen into the human lung would induce local inflammatory and immune responses in antigen-exposed sites. To test this hypothesis, segmental instillation of a well-characterized, highly immunogenic, soluble antigen, keyhole limpet hemocyanin (KLH) was...

journal_title：American journal of respiratory cell and molecular biology

authors： Weissman DN,Bice DE,Crowell RE,Schuyler MR

更新日期：1994-11-01 00:00:00

abstract：:Acute lung injury (ALI) is a devastating disease with an overall mortality rate of 30 to 40%. The coagulation/fibrinolysis system is implicated in the pathogenesis of ALI. Thrombin-activatable fibronolysis inhibitor (TAFI) is an important component of the fibrinolysis system. Recent studies have shown that the active ...

journal_title：American journal of respiratory cell and molecular biology

authors： Naito M,Taguchi O,Kobayashi T,Takagi T,D'Alessandro-Gabazza CN,Matsushima Y,Boveda-Ruiz D,Gil-Bernabe P,Matsumoto T,Chelakkot-Govindalayathil AL,Toda M,Yasukawa A,Hataji O,Morser J,Takei Y,Gabazza EC

更新日期：2013-10-01 00:00:00

abstract：:Recent evidence from genetic experiments in yeast and from studies using guanosine triphosphate (GTP) analogues in mammalian cells suggests a key role for low-molecular-mass GTP-binding proteins (LMM-GBPs) (Mr 19 to 28 kD) in processes of intracellular vesicular sorting and secretion. Assembly and exocytosis of the la...

journal_title：American journal of respiratory cell and molecular biology

authors： Rubins JB,Panchenko M,Shannon TM,Dickey BF

更新日期：1992-03-01 00:00:00

abstract：:We investigated the expression and function of matrix metalloproteinase-12 (MMP-12) in a model of allergic airway inflammation. Mice were sensitized mucosally by exposure to aerosolized ovalbumin (OVA) daily over a period of 10 d in the context of adenovirus-mediated granulocyte macrophage colony-stimulating factor (G...

journal_title：American journal of respiratory cell and molecular biology

authors： Pouladi MA,Robbins CS,Swirski FK,Cundall M,McKenzie AN,Jordana M,Shapiro SD,Stämpfli MR

更新日期：2004-01-01 00:00:00

abstract：:The genetic basis for the underlying individual susceptibility to chlorine-induced acute lung injury is unknown. To uncover the genetic basis and pathophysiological processes that could provide additional homeostatic capacities during lung injury, 40 inbred murine strains were exposed to chlorine, and haplotype associ...

journal_title：American journal of respiratory cell and molecular biology

authors： Leikauf GD,Pope-Varsalona H,Concel VJ,Liu P,Bein K,Berndt A,Martin TM,Ganguly K,Jang AS,Brant KA,Dopico RA Jr,Upadhyay S,Di YP,Li Q,Hu Z,Vuga LJ,Medvedovic M,Kaminski N,You M,Alexander DC,McDunn JE,Prows DR,Kn

更新日期：2012-08-01 00:00:00

abstract：:Lactosylated poly-L-lysine is a nonviral vector that transfers genes into airway epithelial cells, including those from individuals with cystic fibrosis (CF). Substitution of 40% of the epsilon-amino groups of poly-L-lysine with lactosyl residues not only provided a ligand for receptor-mediated endocytosis, but also r...

journal_title：American journal of respiratory cell and molecular biology

authors： Kollen WJ,Schembri FM,Gerwig GJ,Vliegenthart JF,Glick MC,Scanlin TF

更新日期：1999-05-01 00:00:00

abstract：:Bitter taste receptor (TAS2R) agonists dilate airways by receptor-dependent smooth muscle relaxation. Besides their coupling to relaxation, we have found that human airway smooth muscle (HASM) cell TAS2Rs activate (phosphorylate) extracellular signal-related kinase 1/2 (ERK1/2), but the cellular effects are not known....

journal_title：American journal of respiratory cell and molecular biology

authors： Kim D,Cho S,Castaño MA,Panettieri RA,Woo JA,Liggett SB

更新日期：2019-05-01 00:00:00

abstract：:After localized lung immunization and challenge, antigen-specific antibody continues to be produced in the immunized lung lobes of dogs for years after the last antigen exposure. Lavage fluid from immunized lung lobes contains significantly more antigen-specific antibody than lavage fluid from control lung lobes, and ...

journal_title：American journal of respiratory cell and molecular biology

authors： Bice DE,Jones SE,Muggenburg BA

更新日期：1993-06-01 00:00:00

abstract：:Mucus hypersecretion is characteristic of chronic airway diseases. However, regulatory mechanisms are poorly understood. Human airway epithelial cells grown on permeable supports at the air-liquid interface (ALI) develop a mucociliary morphology resembling that found in vivo. Such cultures provide a model for studying...

journal_title：American journal of respiratory cell and molecular biology

authors： Bernacki SH,Nelson AL,Abdullah L,Sheehan JK,Harris A,Davis CW,Randell SH

更新日期：1999-04-01 00:00:00