Abstract:
:The introduction of foreign genes into cells has become an effective means of achieving intracellular expression of foreign proteins, both for therapeutic purposes and for experimental manipulation. Gene delivery to the nervous system has been extensively studied, primarily using viral vectors. However, to date less work has focused on gene delivery to the inner ear, and existing studies have primarily used adenovirus and adeno-associated virus. Using two recombinant viral vectors, herpes simplex type 1 (HSV-1), and vaccinia virus, bearing the Escherichia coli lacZ gene, we tested gene delivery to the guinea pig cochlea in vivo with beta-galactosidase staining as an assay. The HSV-1 and vaccinia virus vectors were both found to infect and elicit transgene expression successfully in many cells in the guinea pig cochlea, including cells in the organ of Corti. These data demonstrate the feasibility of gene delivery to the inner ear using these two viral vectors. Such techniques may facilitate study of the auditory systems, and might be used to develop gene therapy strategies for some forms of hearing loss.
journal_name
Hear Resjournal_title
Hearing researchauthors
Derby ML,Sena-Esteves M,Breakefield XO,Corey DPdoi
10.1016/s0378-5955(99)00045-3keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
1-8issue
1-2eissn
0378-5955issn
1878-5891pii
S0378-5955(99)00045-3journal_volume
134pub_type
杂志文章相关文献
HEARING RESEARCH文献大全abstract::The hair cells of the vertebrate inner ear posses active mechanical processes to amplify their inputs. The stereocilia bundle of various vertebrate animals can produce active movements. Though standard stereocilia-based mechanisms to promote amplification persist in mammals, an additional radically different mechanism...
journal_title:Hearing research
pub_type: 杂志文章,评审
doi:10.1016/j.heares.2009.12.028
更新日期:2011-03-01 00:00:00
abstract::The number, size and distribution of myelinated nerve fibers were analyzed in the osseous spiral lamina (OSL) of young and old gerbils raised in a quiet environment. Because decreased endocochlear potentials (EPs) play a significant role in age-related hearing loss in the gerbil, we correlated morphometric and topogra...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(01)00340-9
更新日期:2001-11-01 00:00:00
abstract::Listening to speech in noise can be effortful but when motivated people seem to be more persevering. Previous research showed effects of monetary reward on autonomic responses like cardiovascular reactivity and pupil dilation while participants processed auditory information. The current study examined the effects of ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2018.07.011
更新日期:2018-09-01 00:00:00
abstract::It is still a matter of debate whether neurons in the higher central nervous system of anurans become progressively more sharply tuned to sounds that have a behavioral importance or that such coding is performed by (small) groups of neurons. The approach we have taken to investigate this matter comprises simultaneous ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(87)90138-9
更新日期:1987-01-01 00:00:00
abstract::Mutations in GJB2 account for the majority of recessive forms of prelingual hearing loss. However, in most previous studies it was not possible to distinguish between congenital (present at birth) and non-congenital prelingual hearing loss. In the present study, the frequency of GJB2 alleles in 20 newborns with bilate...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2004.06.012
更新日期:2004-11-01 00:00:00
abstract::Noise, hearing loss, and electronic signal distortion, which are common problems in military environments, can impair speech intelligibility and thereby jeopardize mission success. The current study investigated the impact that impaired communication has on operational performance in a command and control environment ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2016.10.007
更新日期:2017-06-01 00:00:00
abstract::A model for cochlear mechanics is proposed to take account of its two systems, one passive and one active. The classical passive system stimulates the inner hair cells directly at levels above about 40 dB SL. At intensities below about 60 dB an active process, the 'cochlear amplifier' (CA), somehow provides additional...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(83)90136-3
更新日期:1983-01-01 00:00:00
abstract::Cochleotopic representation in cortex (AI) is extensively reorganized in cats having neonatal, bilateral high frequency cochlear hearing loss. Anterior areas of AI, normally devoted to high frequencies, contain neurons which are almost all tuned to one lower frequency. This frequency corresponds, at the level of the c...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(91)90131-r
更新日期:1991-07-01 00:00:00
abstract::The auditory filter may be considered as a weighting function representing frequency selectivity at a particular centre frequency. Its shape can be derived using the power-spectrum model of masking which assumes: (1) in detecting a signal in a masker the observer uses the single auditory filter giving the highest sign...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(87)90050-5
更新日期:1987-01-01 00:00:00
abstract::Auditory-nerve excitation by two response factors that can be in antiphase has been hypothesized by Kiang (1990) on the basis of non-linear interference in responses to tones (Kiang et al., 1969). The general conditions for antiphasic responses and the relevance of the hypothesis for other auditory stimuli are unknown...
journal_title:Hearing research
pub_type: 杂志文章,评审
doi:10.1016/0378-5955(95)00154-7
更新日期:1995-10-01 00:00:00
abstract::Kanamycin (KM)-induced changes in expression of the gene for glutamate-aspartate transporter (GLAST) in the rat cochlea were analyzed by Northern blotting. With the administration of KM (600 mg/kg/day) once daily for 20 days, the expression of GLAST mRNA gradually increased and reached a peak on day 20. Although the e...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(99)00050-7
更新日期:1999-07-01 00:00:00
abstract::The columnar and layer-specific response properties of neurons in the primary auditory cortex (AI) of Mongolian gerbils were studied using single-unit recordings of responses to tone-burst stimuli presented to the ear contralateral to the recording side. During near-radial microelectrode penetrations of the AI in 100-...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(97)00119-6
更新日期:1997-10-01 00:00:00
abstract::The radial width of three rows of cuticular plates of outer hair cells (width of the OHC triad), the distribution of inner hair cells along the organ of Corti, and postnatal maturation of the middle ear were examined in rats of the Srague-Dawley and Lewis strains aged 0-24 days and two months. The width of the OHC tri...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(85)90064-4
更新日期:1985-03-01 00:00:00
abstract::The phase angle, alpha, between a tonal signal and a tonal masker was varied from 0 degrees to 135 degrees in simultaneous masking, forward masking, and pulsation-threshold paradigms. In all conditions the frequency of the signal and masker was 500 Hz. For forward masking both diotic (MOSO) and dichotic (MOS pi) liste...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(82)90039-9
更新日期:1982-08-01 00:00:00
abstract::Cochlear implant insertion should be as least traumatic as possible in order to reduce trauma to the cochlear sensory structures. The force applied to the cochlea during array insertion should be controlled to limit insertion-related damage. The relationship between insertion force and histological traumatism remains ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2016.11.002
更新日期:2017-02-01 00:00:00
abstract::This report describes a study that took advantage of the unique reactivity of the patas monkey (Erythrocebus patas) to dihydrostreptomycin-sulfate (DHSM) to investigate the effects of selective outer hair cell (OHC) lesions on psychophysical tuning curves (PTC). Four patas monkeys were trained using operant reinforcem...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(87)90161-4
更新日期:1987-01-01 00:00:00
abstract::Auditory cortex exhibit a capacity of modulating the functions of subcortical auditory nuclei and even inner ear through descending pathways. The cochlear nucleus (CN), which acts as the gateway from the auditory periphery to the central auditory system, is also subjected to corticofugal modulation. Cortical modulatio...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2018.12.013
更新日期:2019-03-01 00:00:00
abstract::The effects of several hours of continuous electrical stimulation in the cats' cochlear nucleus with chronically implanted activated iridium microelectrodes was investigated from the changes in the evoked response near the inferior colliculus and also by histologic evaluation of the stimulated tissue. The stimulating ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(92)90201-w
更新日期:1992-09-01 00:00:00
abstract::Temporary threshold shifts (TTS), of the cochlear action potential in the guinea pig, caused by a high frequency exposure tone could be reduced by a prior exposure at the same frequency but lower intensity. The TTS caused by the initial, low intensity exposure tone was allowed to recover to levels where the thresholds...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(83)90105-3
更新日期:1983-11-01 00:00:00
abstract::The purpose of this study was to compare cortical brain responses evoked by amplitude modulated acoustic beats of 3 and 6 Hz in tones of 250 and 1000 Hz with those evoked by their binaural beats counterparts in unmodulated tones to indicate whether the cortical processes involved differ. Event-related potentials (ERPs...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2010.01.013
更新日期:2010-04-01 00:00:00
abstract::The latency, the rise time and the influence of the acoustic reflex on sound transmission were investigated in the adult rat during ketamin anesthesia. This was done by recordings of the cochlear microphonics (CM) and electromyographic (EMG) recordings of the reflex responses of the tensor tympani muscle. The acoustic...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(90)90061-s
更新日期:1990-10-01 00:00:00
abstract::To rehabilitate profoundly deaf patients who are not suitable for cochlear implants, central auditory prostheses have been implanted. To compare two possible electrode configurations - penetrating and surface ones - electrical stimulation of the cochlear nucleus with both types of arrays was tested on guinea pigs and ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(97)00170-6
更新日期:1997-12-01 00:00:00
abstract::Synaptic bodies (SBs) associated with rings of synaptic vesicles and well-defined, pre- and post-synaptic membrane structures are indicators of maturity in most hair cell-afferent nerve junctions. The role of the SBs remains elusive despite several experiments showing that they may be involved in storage of neurotrans...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(93)90135-n
更新日期:1993-04-01 00:00:00
abstract::The pupil dilation response is sensitive both to listening effort and the emotional significance of a task. We aimed to assess the influence of evaluative feedback on the pupil dilation response using a speech reception threshold (SRT) task. Besides the pupil dilation response, we acquired subjective ratings and two p...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2019.07.005
更新日期:2019-09-15 00:00:00
abstract::The field L complex is thought to be the highest auditory centre and the input in the song vocal nuclei. Different anatomical and functional subdivisions have been described in field L. Auditory neurons of field L are well activated by natural sounds and especially by species-specific sounds. A complex sound coding ap...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2005.01.008
更新日期:2005-09-01 00:00:00
abstract::We investigated the effects of intracochlear electrical stimulation (ICES) on auditory pathways of neonatal rat deafened by daily amikacin injections. Expression of mRNAs encoding ionotropic glutamate receptor subunits such as alpha-amino-3-hydroxy-5-methyl-4-isoxazole (AMPA) and N-methyl-D-aspartate (NMDA), and gamma...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(00)00167-2
更新日期:2000-12-01 00:00:00
abstract::Mutations of the otoferlin gene have been shown to underlie deafness disorders in humans and mice. Analyses of genetically engineered mice lacking otoferlin have demonstrated an essential role for this protein in vesicle exocytosis at the inner hair cell afferent synapse. Here, we report on the molecular and phenotypi...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2007.09.005
更新日期:2007-12-01 00:00:00
abstract::A novel immunocytochemical method was used to determine whether the sound-damaged adult quail ear can repair its tectorial membrane (TM) and to compare the repair in quail to that in chicks. Birds were exposed to an octave band noise with a center frequency of 1.5 kHz at 116 dB SPL for 4 h. The chicks were grouped bas...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(95)00051-5
更新日期:1995-06-01 00:00:00
abstract::Sustained vocalizations of vowels [a], [i], and syllable [mə] were collected in twenty normal-hearing individuals. On vocalizations, five conditions of different audio-vocal feedback were introduced separately to the speakers including no masking, wearing supra-aural headphones only, speech-noise masking, high-pass no...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2015.02.005
更新日期:2015-06-01 00:00:00
abstract::A growing body of evidence implies that the dorsal cochlear nucleus (DCN) plays an important role in tinnitus. To test the hypothesis that the rostral output of the DCN is necessary for the experience of chronic tinnitus, the dorsal DCN and the dorsal acoustic stria of rats with psychophysical evidence of tinnitus was...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2004.12.013
更新日期:2005-08-01 00:00:00