Abstract：
:Functional studies of different human cell types have been successfully conducted under in vitro conditions. Despite many efforts, it has not been possible to develop a human myocardial preparation in which contractile function can be studied over several days. We hypothesize that by mimicking the in vivo situation in an in vitro environment we can preserve viability and function of human myocardial preparations over several days. From explanted hearts of patients undergoing cardiac transplantation, multicellular preparations were dissected and mounted in a sterile muscle chamber. Muscles were stimulated at 0.5 or 1 Hz at 1.75 mmol/l Ca(2+), a pH of 7.4 and at 37 degrees C, and kept contracting isometrically for 2-6 days. This study shows for the first time that contractile function of human myocardial tissue can be preserved over several days; active force development had not significantly changed after 48 h (10.6+/-1.2 at t=0 v 11.4+/-2.8 mN/mm(2)at t=48, n=10), nor had diastolic force (1.0+/-0.1 v 0.9+/-0.1 mN/mm(2)). After at least 48 h, the contractile response to stimulation with 1 micromol/l isoproterenol was clearly present: developed force increased to 631+/-146% of control values, while half-relaxation time declined to 57+/-6% (n=7). In addition, both pharmacological responses and regulatory physiological behavior, such as post-rest potentiation and force-frequency relationships, are preserved. This technique allows the study of the regulation of contractile function of human myocardium in vitro and may be used to link changes in protein expression to consequent changes in myocardial contraction.
journal_name
J Mol Cell Cardioljournal_title
Journal of molecular and cellular cardiologyauthors
Janssen PM,Lehnart SE,Prestle J,Hasenfuss Gdoi
10.1006/jmcc.1999.0978keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
1419-27issue
8eissn
0022-2828issn
1095-8584pii
S0022-2828(99)90978-1journal_volume
31pub_type
杂志文章abstract：:This study examined the potential role of ET-1 and the contribution of protein kinase C (PKC) in the desensitization of the ET-1 transmembrane signaling pathway in the left circumflex coronary artery (CCA) of a dog model of congestive heart failure (CHF). In the CCA of the rapid ventricular pacing-overdrive dog model ...
journal_title：Journal of molecular and cellular cardiology
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journal_title：Journal of molecular and cellular cardiology
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1006/jmcc.1996.0053
更新日期：1996-03-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2013.03.014
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1006/jmcc.2000.1287
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2009.11.011
更新日期：2010-04-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1006/jmcc.1993.1088
更新日期：1993-07-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/s0022-2828(88)80130-5
更新日期：1988-05-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章,评审
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更新日期：2008-01-01 00:00:00
abstract：:Recent observations challenged the functional importance or even the existence of mitochondrial ATP-dependent K+ (mitoK(ATP)) channels. In the present study, we determined the presence of K(ATP)-channel subunits in mouse heart mitochondria, and investigated whether known openers or blockers of the channel can alter mi...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/s0022-2828(03)00249-9
更新日期：2003-11-01 00:00:00
abstract：:While much is known about the deleterious effects of pro-inflammatory cytokines on development of vascular disease, little is reported on the direct effects of anti-inflammatory cytokines on the vascular smooth muscle cell (VSMC) response to injury. Interleukin-19 (IL-19) is a recently described Th2, anti-inflammatory...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2010.04.016
更新日期：2010-10-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章,评审
doi：10.1016/j.yjmcc.2014.06.015
更新日期：2014-10-01 00:00:00
abstract：:Ca2+ serves as a ubiquitous second messenger mediating a variety of cellular processes including electrical excitation, contraction, gene expression, secretion, cell death and others. The identification of the molecular components of the mitochondrial Ca2+ influx and efflux pathways has created a resurgent interest in...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2020.11.014
更新日期：2020-12-05 00:00:00
abstract：:Application of ultrasound-mediated destruction of microbubbles (US + Bubble) to skeletal muscle creates capillary ruptures leading to leakage of the cell components. We studied whether US + Bubble combined with bone-marrow-derived mononuclear cells (BM-MNCs) infusion enables the targeted delivery of endothelial-lineag...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2006.03.012
更新日期：2006-06-01 00:00:00
abstract：:The importance of the Na+/K+/Cl- co-transport system of the rat myocardial sarcolemma was studied under hypothermic ischemia by investigating the effect of the co-transport blockers furosemide and bumetanide on the sodium influx into the myocardium. The intracellular Na+ accumulation during hypothermic ischemia was fo...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1006/jmcc.1993.1157
更新日期：1993-12-01 00:00:00
abstract：:Fibrosis, which is defined as excessive accumulation of fibrous connective tissue, contributes to the pathogenesis of numerous diseases involving diverse organ systems. Cardiac fibrosis predisposes individuals to myocardial ischemia, arrhythmias and sudden death, and is commonly associated with diastolic dysfunction. ...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2013.12.013
更新日期：2014-02-01 00:00:00
abstract：:The ultrastructure of myocardial tissue was studied during ischemia and reperfusion and during reperfusion with a calcium-containing solution after a short period of calcium-free perfusion (calcium paradox). After ischemia an aggregation of the sarcolemmal intramembranous particles was observed. Subsequent reperfusion...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/0022-2828(88)90335-5
更新日期：1988-03-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1006/jmcc.1996.0067
更新日期：1996-04-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/0022-2828(95)90002-0
更新日期：1995-09-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2006.03.426
更新日期：2006-07-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2014.11.012
更新日期：2015-02-01 00:00:00
abstract：:Adult rat heart was dissociated into a single-cell suspension by a retrograde perfusion technique with collagenase and hyaluronidase in Krebs-Ringer phosphate buffer. Long-term culture of these isolated single cardiac muscle cells was established for up to 45 days. Transmission electron microscopy and immunofluorescen...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/0022-2828(83)91342-1
更新日期：1983-05-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1006/jmcc.2000.1174
更新日期：2000-08-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2005.06.019
更新日期：2006-02-01 00:00:00
abstract：:The sympathetic nervous system is the main stimulator of cardiac function. While acute activation of the β-adrenoceptors exerts positive inotropic and lusitropic effects by increasing cAMP and Ca2+, chronically enhanced sympathetic tone with changed β-adrenergic signaling leads to alterations of gene expression and re...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
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更新日期：2019-02-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1006/jmcc.1994.1046
更新日期：1994-03-01 00:00:00
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journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/j.yjmcc.2011.09.012
更新日期：2011-12-01 00:00:00
abstract：:Left ventricular pressure overload will result in the hypertrophic growth of the myocardium and in the rat may include a remodeling of both the structural and contractile proteins. As a result, an adaptive rise in active stiffness, or the force generating capacity of the myocardium, may occur. The relative importance ...
journal_title：Journal of molecular and cellular cardiology
pub_type： 杂志文章
doi：10.1016/0022-2828(88)90597-4
更新日期：1988-12-01 00:00:00
