当前位置： SCI文献检索 > JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY期刊下所有文献 > cAMP-induced actin cytoskeleton remodelling inhibits MKL1-dependent expression of the chemotactic and pro-proliferative factor, CCN1.

cAMP-induced actin cytoskeleton remodelling inhibits MKL1-dependent expression of the chemotactic and pro-proliferative factor, CCN1.

Abstract：

:Elevation of intracellular cAMP concentration has numerous vascular protective effects that are in part mediated via actin cytoskeleton-remodelling and subsequent regulation of gene expression. However, the mechanisms are incompletely understood. Here we investigated whether cAMP-induced actin-cytoskeleton remodelling modulates VSMC behaviour by inhibiting expression of CCN1. In cultured rat VSMC, CCN1-silencing significantly inhibited BrdU incorporation and migration in a wound healing assay. Recombinant CCN1 enhanced chemotaxis in a Boyden chamber. Adding db-cAMP, or elevating cAMP using forskolin, significantly inhibited CCN1 mRNA and protein expression in vitro; transcriptional regulation was demonstrated by measuring pre-spliced CCN1 mRNA and CCN1-promoter activity. Forskolin also inhibited CCN1 expression in balloon injured rat carotid arteries in vivo. Inhibiting RhoA activity, which regulates actin-polymerisation, by cAMP-elevation or pharmacologically with C3-transferase, or inhibiting its downstream kinase, ROCK, with Y27632, significantly inhibited CCN1 expression. Conversely, expression of constitutively active RhoA reversed the inhibitory effects of forskolin on CCN1 mRNA. Furthermore, CCN1 mRNA levels were significantly decreased by inhibiting actin-polymerisation with latrunculin B or increased by stimulating actin-polymerisation with Jasplakinolide. We next tested the role of the actin-dependent SRF co-factor, MKL1, in CCN1 expression. Forskolin inhibited nuclear translocation of MKL1 and binding of MKL1 to the CCN1 promoter. Constitutively-active MKL1 enhanced basal promoter activity of wild-type but not SRE-mutated CCN1; and prevented forskolin inhibition. Furthermore, pharmacological MKL-inhibition with CCG-1423 significantly inhibited CCN1 promoter activity as well as mRNA and protein expression. Our data demonstrates that cAMP-induced actin-cytoskeleton remodelling regulates expression of CCN1 through MKL1: it highlights a novel cAMP-dependent mechanism controlling VSMC behaviour.

journal_name

J Mol Cell Cardiol

journal_title

Journal of molecular and cellular cardiology

authors

Duggirala A,Kimura TE,Sala-Newby GB,Johnson JL,Wu YJ,Newby AC,Bond M

doi

10.1016/j.yjmcc.2014.11.012

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

157-68

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(14)00372-1

journal_volume

79

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY文献大全
  • Functional characterization of the novel DES mutation p.L136P associated with dilated cardiomyopathy reveals a dominant filament assembly defect.

    abstract：BACKGROUND:Dilated cardiomyopathy (DCM) could be caused by mutations in more than 40 different genes. However, the pathogenic impact of specific mutations is in most cases unknown complicating the genetic counseling of affected families. Therefore, functional studies could contribute to distinguish pathogenic mutations...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2015.12.015

    authors： Brodehl A,Dieding M,Biere N,Unger A,Klauke B,Walhorn V,Gummert J,Schulz U,Linke WA,Gerull B,Vorgert M,Anselmetti D,Milting H

    更新日期：2016-02-01 00:00:00

  • Caspase activation and mitochondrial cytochrome C release during hypoxia-mediated apoptosis of adult ventricular myocytes.

    abstract：:Oxygen deprivation for prolonged periods leads to cardiac cell death and ventricular dysfunction. The ability to prevent myocardial cell death would be of significant therapeutic value in maintaining cardiac function after injury. While caspases have been suggested to play a critical role in apoptosis, their involveme...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1999.1057

    authors： de Moissac D,Gurevich RM,Zheng H,Singal PK,Kirshenbaum LA

    更新日期：2000-01-01 00:00:00

  • The interaction of phenylalkylamine calcium channel blockers with the 1,4-dihydropyridine binding site.

    abstract：:The interaction of verapamil and other phenylalkylamine calcium channel blockers with the 1,4-dihydropyridine receptor was examined. Studies characterizing the interaction and relationship between calcium channel blocking potency and binding affinity were performed in rat myocardium. The 1,4-dihydropyridines, nifedipi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80010-4

    authors： Zobrist RH,Giacomini KM,Nelson WL,Giacomini JC

    更新日期：1986-09-01 00:00:00

  • Left ventricular hemodynamic parameters in the course of acute experimental coxsackievirus B 3 myocarditis.

    abstract：:In susceptible DBA/2 mice coxsackievirus B 3-induced myocarditis leads to inflammatory and necrotic lesions in the myocardium 7-10 days after virus inoculation. The purpose of this study was to determine whether hemodynamic changes occur in murine coxsackievirus B 3 myocarditis and whether they are correlated to histo...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(95)90425-5

    authors： Herzum M,Weller R,Jomaa H,Wietrzychowski F,Pankuweit S,Mahr P,Maisch B

    更新日期：1995-08-01 00:00:00

  • Structural vs. contractile protein remodeling and myocardial stiffness in hypertrophied rat left ventricle.

    abstract：:Left ventricular pressure overload will result in the hypertrophic growth of the myocardium and in the rat may include a remodeling of both the structural and contractile proteins. As a result, an adaptive rise in active stiffness, or the force generating capacity of the myocardium, may occur. The relative importance ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(88)90597-4

    authors： Jalil JE,Doering CW,Janicki JS,Pick R,Clark WA,Abrahams C,Weber KT

    更新日期：1988-12-01 00:00:00

  • Prevention of abnormal sarcoplasmic reticulum calcium transport and protein expression in post-infarction heart failure using 3, 5-diiodothyropropionic acid (DITPA).

    abstract：:Heart failure of diverse causes is associated with abnormalities of sarcoplasmic reticulum (SR) Ca(2+)transport. The purpose of this study was to determine whether the thyroid hormone analogue, 3,5-diiodothyropropionic acid (DITPA), prevents abnormal Ca(2+)transport and expression of SR proteins associated with post-i...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2000.1225

    authors： Pennock GD,Spooner PH,Summers CE,Litwin SE

    更新日期：2000-11-01 00:00:00

  • The feedback loop of "EMMPRIN/NF-κB" worsens atherosclerotic plaque via suppressing autophagy in macrophage.

    abstract：:This study examined the significance of macrophage autophagy in extracellular matrix metalloproteinase inducer (EMMPRIN)-mediated atherosclerosis (AS). Apolipoprotein E-deficient (ApoE-/-) mice were fed a western diet to establish an AS model. EMMPRIN and p62/Sequestosome-1(SQSTM1) expression were evaluated in plaque ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2017.11.008

    authors： Liang X,Hou X,Yang Y,Liu H,Guo R,Yang Z,Yang L

    更新日期：2018-01-01 00:00:00

  • Altered expression of titin and contractile proteins in failing human myocardium.

    abstract：:Our own previous ultrastructural studies in human hearts with dilated cardiomyopathy and heart failure showed sarcomeric and cytoskeletal disarrangement. On the basis of these findings we tested the hypothesis that in cardiomyopathic failing hearts not only the sarcomere structure but also the organization and the amo...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1148

    authors： Hein S,Scholz D,Fujitani N,Rennollet H,Brand T,Friedl A,Schaper J

    更新日期：1994-10-01 00:00:00

  • Genetic modification of the heart: chaperones and the cytoskeleton.

    abstract：:In the past decade, genetic modification has been extensively employed to define (patho)physiological roles of chaperones and the cytoskeleton in the heart, promoting dramatic advances in this field. Both loss-of-function and gain-of-function approaches have been used productively. alphaB-Crystallin (CryAB) is the mos...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2004.07.004

    authors： Kumarapeli AR,Wang X

    更新日期：2004-12-01 00:00:00

  • Gender modulation of Ca(2+) uptake in cardiac mitochondria.

    abstract：BACKGROUND:Mitochondrial calcium overload is an important factor in defining ischemia/reperfusion injury. Since pre-menopausal women are relatively protected from ischemia and heart disease, we tested the hypothesis that gender differences alter Ca(2+) handling in rat cardiac mitochondria. METHODS:Using cardiac mitoch...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2004.04.023

    authors： Arieli Y,Gursahani H,Eaton MM,Hernandez LA,Schaefer S

    更新日期：2004-08-01 00:00:00

  • Quantifying antiarrhythmic drug blocking during action potentials in guinea-pig papillary muscle.

    abstract：:This report examines the blocking effects that several antiarrhythmic drugs have on rapid upstroke velocities of action potentials recorded from guinea-pig papillary muscle. A mathematical analysis is developed, that measures an effective "blocking rate' that acts during the action potential with the purpose being to ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(83)90334-6

    authors： Courtney KR

    更新日期：1983-11-01 00:00:00

  • Electrophysiologic and extracellular ionic changes during acute ischemia in failing and normal rabbit myocardium.

    abstract：:The incidence of ventricular arrhythmias is higher in failing hearts than in control hearts, especially during acute ischemia. Electrophysiological and extracellular ionic changes during acute ischemia in normal and failing rabbit myocardium were assessed. Heart failure was induced in rabbits by combined volume and pr...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0012

    authors： Vermeulen JT,Tan HL,Rademaker H,Schumacher CA,Loh P,Opthof T,Coronel R,Janse MJ

    更新日期：1996-01-01 00:00:00

  • Alterations in the diabetic myocardial proteome coupled with increased myocardial oxidative stress underlies diabetic cardiomyopathy.

    abstract：:Diabetic cardiomyopathy has been documented as an underlying etiology of heart failure (HF) among diabetics. Although oxidative stress has been proposed to contribute to diabetic cardiomyopathy, much of the evidence lacks specificity. Furthermore, whether alterations occur at the cardiac proteome level in diabetic car...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2006.12.018

    authors： Hamblin M,Friedman DB,Hill S,Caprioli RM,Smith HM,Hill MF

    更新日期：2007-04-01 00:00:00

  • Evidence that translocation of protein kinase C is a key event during ischemic preconditioning of rabbit myocardium.

    abstract：:We used three interventions to test critically the theory that ischemic preconditioning is the result of translocation of cytosolic protein kinase C (PKC) into the membranes where it can be activated. If that theory were true then kinase activity should not be necessary during the preconditioning ischemia and thus blo...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1078

    authors： Liu Y,Ytrehus K,Downey JM

    更新日期：1994-05-01 00:00:00

  • Extracellular matrix remodeling in atrial fibrosis: mechanisms and implications in atrial fibrillation.

    abstract：:Atrial fibrosis has been strongly associated with the presence of heart diseases/arrhythmias, including congestive heart failure (CHF) and atrial fibrillation (AF). Inducibility of AF as a result of atrial fibrosis has been the subject of intense recent investigation since it is the most commonly encountered arrhythmi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2009.09.001

    authors： Pellman J,Lyon RC,Sheikh F

    更新日期：2010-03-01 00:00:00

  • The effects of triiodothyronine on cultured neonatal rat cardiac myocytes.

    abstract：:The purpose of this study was to determine whether thyroid hormone could directly affect the phenotypic expression of two isozymic systems [lactate dehydrogenase (LDH) and myosin] and the energy transducing potential of cultured neonatal heart cells. In addition we determined if these biochemical systems developed in ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(88)80014-2

    authors： Williams HM,Ianuzzo CD

    更新日期：1988-08-01 00:00:00

  • Germline hereditary, somatic mutations and microRNAs targeting-SNPs in congenital heart defects.

    abstract：:Somatic mutations and dysregulation by microRNAs (miRNAs) may have a pivotal role in the Congenital Heart Defects (CHDs). The purpose of the study was to assess both somatic and germline mutations in the GATA4 and NKX2.5 genes as well as to identify 3'UTR single nucleotide polymorphisms (SNPs) in the miRNA target site...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.yjmcc.2013.04.002

    authors： Sabina S,Pulignani S,Rizzo M,Cresci M,Vecoli C,Foffa I,Ait-Ali L,Pitto L,Andreassi MG

    更新日期：2013-07-01 00:00:00

  • Pyruvate attenuation of hypoxia damage in isolated working guinea-pig heart.

    abstract：:Function and various parameters of myocardial substrate and energy metabolism were measured in preload-controlled isolated working guinea-pig hearts perfused with normoxic (95% O2) and hypoxic (30 to 45% O2) Krebs-Henseleit buffers ([Ca2+] = 1.25 mM). Energy-yielding substrates were glucose, pyruvate, lactate, and fat...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80905-1

    authors： Bünger R,Swindall B,Brodie D,Zdunek D,Stiegler H,Walter G

    更新日期：1986-04-01 00:00:00

  • Diabetes and susceptibility to reperfusion-induced ventricular arrhythmias.

    abstract：:Studies using chemically-induced models of diabetes have shown the diabetic myocardium to exhibit abnormalities in cellular ion transport, which may affect susceptibility to reperfusion-induced arrhythmias. We studied the incidence of reperfusion-induced ventricular tachycardia (VT) and fibrillation (VF) in isolated h...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(92)93195-p

    authors： Kusama Y,Hearse DJ,Avkiran M

    更新日期：1992-04-01 00:00:00

  • Depressed Frank-Starling mechanism in the left ventricular muscle of the knock-in mouse model of dilated cardiomyopathy with troponin T deletion mutation ΔK210.

    abstract：:It has been reported that the Frank-Starling mechanism is coordinately regulated in cardiac muscle via thin filament "on-off" equilibrium and titin-based lattice spacing changes. In the present study, we tested the hypothesis that the deletion mutation ΔK210 in the cardiac troponin T gene shifts the equilibrium toward...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2013.07.001

    authors： Inoue T,Kobirumaki-Shimozawa F,Kagemoto T,Fujii T,Terui T,Kusakari Y,Hongo K,Morimoto S,Ohtsuki I,Hashimoto K,Fukuda N

    更新日期：2013-10-01 00:00:00

  • Cardioprotective signaling to mitochondria.

    abstract：:Mitochondria are central players in the pathophysiology of ischemia-reperfusion. Activation of plasma membrane G-coupled receptors or the Na,K-ATPase triggers cytosolic signaling pathways that result in cardioprotection. Our working hypothesis is that the occupied receptors migrate to caveolae, where signaling enzymes...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2008.11.019

    authors： Garlid KD,Costa AD,Quinlan CL,Pierre SV,Dos Santos P

    更新日期：2009-06-01 00:00:00

  • Chronic inhibition of Rho kinase blunts the process of left ventricular hypertrophy leading to cardiac contractile dysfunction in hypertension-induced heart failure.

    abstract：:The Gq-RhoA-Rho kinase pathway, activated by neurohormonal factors such as angiotensin II (Ang II), has been proposed to be one of the important signaling pathways involved in the progression of left ventricular (LV) hypertrophy to heart failure. We tested the hypothesis that chronic inhibition of Rho kinase prevents ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(02)00278-x

    authors： Satoh S,Ueda Y,Koyanagi M,Kadokami T,Sugano M,Yoshikawa Y,Makino N

    更新日期：2003-01-01 00:00:00

  • Non-coding RNAs: Regulators of valvular calcification.

    abstract：:There is currently a growing global burden of valvular heart diseases due to aging populations and changing lifestyles. Valvular heart diseases mainly include the malfunctioning of aortic and mitral valves and are characterized by extensive tissue remodeling, which includes calcification, endothelial dysfunction, and ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2020.03.015

    authors： Gupta SK,Kumari S,Singh S,Barthwal MK,Singh SK,Thum T

    更新日期：2020-05-01 00:00:00

  • Subcellular localization of Na/K-ATPase isoforms in ventricular myocytes.

    abstract：:The sodium/potassium ATPase (NKA) is essential for establishing the normal intracellular [Na+] and [K+] and transmembrane gradients that are essential for many cellular functions, including cardiac electrophysiology and contractility. Different NKA isoforms exhibit differential expression levels, cellular localization...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2017.05.013

    authors： Yuen GK,Galice S,Bers DM

    更新日期：2017-07-01 00:00:00

  • Dioctanoyl-glycerol inhibits L-type calcium current in embryonic chick cardiomyocytes independent of protein kinase C activation.

    abstract：:Diacylglycerol analogs and phorbol esters are used as protein kinase C (PKC) activators to investigate the effect of PKC on L-type calcium current [ICa(L)] in cardiomyocytes. 1-2-dioctanoyl-sn-glycerol (diC8) is a potent analog of diacylglycerol (DAG) which produces positive inotropic effects in guinea-pig atria cardi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(95)90058-6

    authors： Conforti L,Sumii K,Sperelakis N

    更新日期：1995-05-01 00:00:00

  • Glibenclamide does not prevent action potential shortening induced by ischemia in anesthetized rabbits but reduces ischemia-induced arrhythmias.

    abstract：:The possible ischemia-selective Class III anti-arrhythmic action (selective action potential widening in ischemia) of the IKATP blocker glibenclamide was assessed in anesthetized rabbits during ischemia induced by complete occlusion of a coronary artery. Coronary artery occlusion caused an initial prolongation in mono...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1998.0664

    authors： Barrett TD,Walker MJ

    更新日期：1998-05-01 00:00:00

  • Metabolic remodeling in the aging heart.

    abstract：:The aim of this study was to investigate the effects of aging on the profile of myocardial substrate utilization and cardiac function using a physiological profile of substrates. Hearts from 6-, 15- and 24-month male Wistar rats were perfused in the isovolumic Langendorff mode, with physiological concentrations of 13C...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2005.09.018

    authors： Sample J,Cleland JG,Seymour AM

    更新日期：2006-01-01 00:00:00

  • Inhibition of sodium influx and improved preservation of rat hearts during hypothermic ischemia by furosemide and bumetanide: a 23Na- and 31P-NMR study.

    abstract：:The importance of the Na+/K+/Cl- co-transport system of the rat myocardial sarcolemma was studied under hypothermic ischemia by investigating the effect of the co-transport blockers furosemide and bumetanide on the sodium influx into the myocardium. The intracellular Na+ accumulation during hypothermic ischemia was fo...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1993.1157

    authors： Rubin Y,Navon G

    更新日期：1993-12-01 00:00:00

  • Human connexin31.9, unlike its orthologous protein connexin30.2 in the mouse, is not detectable in the human cardiac conduction system.

    abstract：:In the human heart connexin(Cx)40, Cx43 and Cx45-containing gap junctional channels electrically couple cardiomyocytes, forming a functional syncytium. In the mouse heart, additionally, Cx30.2-containing gap junctions have been detected in the atrioventricular node where they are implicated, together with Cx45, in imp...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2008.12.007

    authors： Kreuzberg MM,Liebermann M,Segschneider S,Dobrowolski R,Dobrzynski H,Kaba R,Rowlinson G,Dupont E,Severs NJ,Willecke K

    更新日期：2009-04-01 00:00:00

  • Comparative morphometry of the mitochondria and activity of some enzymes in the myocardium of small mammals.

    abstract：:Heart rate in mammals is inversely related to body weight. In the etruscan shrew it exceeds 960 beats/min. Since the heart depends primarily on energy from aerobic sources, it was interesting to examine some morphological parameters of the mitochondria in some mammals with a high heart frequency. Volume fraction and s...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(85)80031-6

    authors： Oron U,Mandelberg M

    更新日期：1985-06-01 00:00:00