Abstract：
PURPOSE:In this study we investigated the need of the support from cumulus cells for germinal-vesicle (GV) oocytes collected from stimulated ovaries to complete their maturation to metaphase II (MII). METHODS:We compared the maturation rate of GV oocytes after coculture with cumulus cells (study group) with their spontaneous maturation in culture medium alone (control group). RESULTS:Sixty-four and nine-tenths percent of the GV oocytes matured to metaphase II in the coculture group, and of these, 43.5% gave normal 2pn zygotes following intracytoplasmic sperm injection (ICSI), while 73.8% of the GV oocytes spontaneously matured to the MII stage and 30% of these reached the zygote stage after ICSI. CONCLUSIONS:It is probable that a follicular factor is responsible for this arrested maturation in the human and that maturation occurs spontaneously when the oocytes are separated from their follicular fluid environment after collection.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Häberle M,Scheurer P,Lauerer K,Fischer M,Hohl MKdoi
10.1023/a:1020410217220keywords:
subject
Has Abstractpub_date
1999-07-01 00:00:00pages
329-31issue
6eissn
1058-0468issn
1573-7330journal_volume
16pub_type
杂志文章abstract：PURPOSE:Our purpose was to compare the diagnostic efficacy of laparoscopy between primary and secondary infertility and to define a subgroup in which the positive findings are low. METHODS:A retrospective review of the cases of 206 infertile women who underwent laparoscopy was conducted. RESULTS:Eighty-two (39.8%) pa...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1023/a:1022578019260
更新日期：1998-10-01 00:00:00
abstract：:The original version of this article unfortunately contained a mistake. The middle initial of Douglas A. Mata was omitted. The original article has been corrected. ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,已发布勘误
doi：10.1007/s10815-017-1093-1
更新日期：2018-02-01 00:00:00
abstract：PURPOSE:To evaluate whether miR-148a-3p overexpression is associated with disrupted decidualization of recurrent implantation failure (RIF). METHODS:Endometrial miRNA and mRNA expression profiles during the implantation window derived from women with and without RIF were identified using microarray and RT-qPCR. Immort...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01900-9
更新日期：2020-10-01 00:00:00
abstract：PURPOSE:This study sought to identify a disease-related gene in a consanguineous Chinese family in which there were two premature ovarian insufficiency (POI) sisters. METHOD:We used whole-exome sequencing and Sanger sequencing to identify the disease-causing gene. Results were verified using an assay of mutant protein...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-019-01675-8
更新日期：2020-02-01 00:00:00
abstract：PURPOSE:Higher serum estradiol levels occur in women undergoing assisted reproductive technology (ART) owing to ovarian stimulation. Here, we investigated the association between maternal serum estradiol levels and the intellectual development of offspring conceived with ART. METHODS:A total of 204 singletons born aft...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-020-01835-1
更新日期：2020-08-01 00:00:00
abstract：PURPOSE:To observe the differences in pregnancy rates (PRs), delivery rates, and abortion rates associated with frozen-embryo-transfer (FET)-based use of post-thawing embryos with different numbers of blastomeres. METHODS:959 FET cycles and 361 successful FET cycles performed between January 2007 and December 2007. Co...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-009-9360-4
更新日期：2009-11-01 00:00:00
abstract：PURPOSE:Fertility treatment in women aged ≥40 year old remains difficult and controversial. All available studies in older women report results of one specific method of ART, i.e. IUI, IVF/ICSI or oocyte donation, and success rates are always published per attempt but never per patient. Randomized studies are not avail...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0085-z
更新日期：2013-11-01 00:00:00
abstract：PURPOSE:This study was designed to evaluate patient management and quality of information given by French oncologists to cancer women concerning fertility issues and possibilities of fertility preservation. METHODS:An online survey was sent to 1161 physicians in all major cancer centers throughout France between May 2...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-018-1251-0
更新日期：2018-10-01 00:00:00
abstract：PURPOSE:Diminished reproductive capacity is a devastating consequence of life-sparing therapies for childhood malignancy. In 2006, the American Society of Clinical Oncology (ASCO) published fertility preservation recommendations (ASCOR) emphasizing the importance of early discussion and intervention for fertility prese...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-010-9504-6
更新日期：2011-03-01 00:00:00
abstract：PURPOSE:The present study by using different growth factors was aimed to develop the best practical culture condition for purification of goat undifferentiated SSCs and their colonization under in vitro and in vivo conditions. METHODS:The enzymatically isolated SSCs obtained from one month old goat testes were culture...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-014-0301-5
更新日期：2014-11-01 00:00:00
abstract：PURPOSE:Our purpose was to show that fertilization of oocytes can be obtained solely by laser light-mediated manipulation of gametes. METHOD:A small channel was drilled into the zona pellucida of bovine oocytes using an ultraviolet (UV)-laser microbeam. Highly diluted cattle sperm were not able to fertilize the laser ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02065947
更新日期：1996-03-01 00:00:00
abstract：PURPOSE:To evaluate the frequency of abnormal prolactin and thyroid stimulating hormone (TSH) test results in ovulatory women with tubal factor infertility who were screened for in vitro fertilization (IVF). METHODS:Charts were identified from 112 ovulatory women with follicle stimulating hormone (FSH) < 20 mIU/ml who...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02211376
更新日期：1995-02-01 00:00:00
abstract：PURPOSE:This study examined sperm motion parameters as measured by computerized automated semen analysis before and after a Percoll wash and determined if differences in any parameter were correlated with fertility subsequent to intrauterine insemination (IUI). RESULTS:Total motile sperm decreased following the washin...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02214129
更新日期：1995-01-01 00:00:00
abstract：OBJECTIVE:To investigate the usefulness of preimplantation genetic diagnosis (PGD) based on mutated allele revealed by sequencing with aneuploidy and linkage analyses (MARSALA) for a pedigree with X-linked retinitis pigmentosa (XLRP). METHODS:One pathogenic mutation (c.494G > A) of the retinitis pigmentosa GTPase regu...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-019-01434-9
更新日期：2019-05-01 00:00:00
abstract：PURPOSE:Determine if estrogen used since the beginning of the menstrual flow could improve endometrial tissue compared to standard endometrial preparation for in vitro maturation cycles. METHODS:Twenty polycystic ovary syndrome women were submitted to two estrogen therapy schedules: standard schedule; estrogen began o...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10815-006-9042-4
更新日期：2006-05-01 00:00:00
abstract：PURPOSE:To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles. METHODS:A total of 160 women who underwent vitrified-warmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10815-013-9984-2
更新日期：2013-06-01 00:00:00
abstract：PURPOSE:To evaluate clinical outcomes of frozen-thawed embryo transfer cycles when one or two blastocysts are transferred. METHODS:Retrospective chart review RESULTS:Two hundred forty-three frozen blastocyst transfer (FBT) cycles were analyzed. Clinical pregnancy rate (50.4% vs. 34.7%), live birth rate (45.8% vs. 30....
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-011-9551-7
更新日期：2011-07-01 00:00:00
abstract：: ...
journal_title：Journal of assisted reproduction and genetics
pub_type： 社论
doi：10.1007/s10815-018-1201-x
更新日期：2018-04-01 00:00:00
abstract：PURPOSE:To generate novel, objective variables that resemble embryo quality and relate them to ongoing implantation, using multilevel imaging of single-transferred embryos. METHODS:Retrospective analysis of multilevel images of 659 day 3 single-transferred embryos. Each embryo was photographed on seven different level...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-9970-8
更新日期：2013-04-01 00:00:00
abstract：PURPOSE:To study the outcome of repeated biopsy for pre-implantation genetic testing in case of failed genetic diagnosis in the first biopsy. METHODS:The study group included 81 cycles where embryos underwent re-biopsy because there were no transferable embryos after the first biopsy: in 55 cycles, the first procedure...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-018-1359-2
更新日期：2019-01-01 00:00:00
abstract：PROBLEM:Intravenous immunoglobulin (IVIG) has been used to suppress autoimmune and inflammatory disorders by a variety of mechanisms. Recently, the CD200 tolerance-promoting signal has been found to play a role in IVIG suppression of blood natural killer (NK) cells. Further, different types of IVIG have been reported t...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-008-9202-9
更新日期：2008-02-01 00:00:00
abstract：PURPOSE:To demonstrate that a euploid embryo derived from an oocyte with reciprocal aneuploid polar bodies is capable of producing a chromosomally normal child. METHODS:A case report of maternal MI error compensation where single nucleotide polymorphism (SNP) microarray based comprehensive chromosome screening (CCS) w...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-012-9746-6
更新日期：2012-06-01 00:00:00
abstract：PURPOSE:The objectives were to test how the source of oocytes and semen impacted vitrification of large numbers of bovine oocytes and subsequent IVF and early embryo development to test procedures that may assist with assisted reproductive technologies in humans. METHODS:Bovine oocytes were vitrified from follicles of...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-009-9362-2
更新日期：2009-11-01 00:00:00
abstract：PURPOSE:To determine the percentage of unbalanced spermatozoa and an interchromosomal effect in two carriers of balanced translocations t(13;15)(q32;q26) and t(13;15)(q32;p11.2). METHODS:Sperm nuclei analysis by fluorescent in situ hybridization for detection of percentage of unbalanced spermatozoa and sperm with diso...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-012-9767-1
更新日期：2012-07-01 00:00:00
abstract：PURPOSE:Polycystic ovary syndrome (PCOS) is a common endocrine disorder disease among women in reproductive-age. Since follicle stimulating hormone (FSH) exerts important biological functions, the association between PCOS and FSH receptor (FSHR) polymorphisms attracts wide attention. The aim of this study was to evalua...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-013-0166-z
更新日期：2014-03-01 00:00:00
abstract：PURPOSE:This clinical study was designed to identify and compare the pregnancy rates of simultaneous program of natural-cycle IVF and cryopreserved-thawed ET (NICE) with those of natural-cycle IVF and cryopreserved-thawed ET. METHODS:All three groups comprised spontaneously ovulating infertile women under the age of 4...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02066425
更新日期：1996-10-01 00:00:00
abstract：PURPOSE:To compare multiple displacement amplification and OmniPlex whole genome amplification technique performance during array comparative genome hybridization (aCGH), Sanger sequencing, SNaPshot and fragment size analysis downstream applications in frame of multifactor embryo preimplantation genetic testing. METHO...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/s10815-018-1187-4
更新日期：2018-08-01 00:00:00
abstract：PURPOSE:We have developed sensitive diagnostic procedures for studies on the normal and mutant alleles of the triplet repeat genes associated with myotonic dystrophy and fragile X in single human somatic cells, gametes and embryos. METHODS:Polymerase chain reaction (PCR) assays for the normal alleles of the myotonic d...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章,评审
doi：10.1007/BF02072539
更新日期：1996-02-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to assess the motility of human sperm incubated with various concentrations of lignocaine. METHODS:Eleven semen samples with a sperm density greater than or equal to 20 x 10(6) ml and progressive motility greater than or equal to 40% were prepared using a swim-up technique. Aliq...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF01203827
更新日期：1992-06-01 00:00:00
abstract：PURPOSE:Our purpose was to investigate the effect on the ovarian response of increasing the gonadotropin dose. METHODS:We analyzed retrospectively the in vitro fertilization data for patients who had two cycles of treatment, with a higher dose in cycle 2. The patients were stratified according to age, ovarian response...
journal_title：Journal of assisted reproduction and genetics
pub_type： 杂志文章
doi：10.1007/BF02744938
更新日期：1998-08-01 00:00:00
