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Vitrification of bovine oocytes: implications of follicular size and sire on the rates of embryonic development.

Abstract：

PURPOSE:The objectives were to test how the source of oocytes and semen impacted vitrification of large numbers of bovine oocytes and subsequent IVF and early embryo development to test procedures that may assist with assisted reproductive technologies in humans. METHODS:Bovine oocytes were vitrified from follicles of different diameters, small (< or =4 mm) and medium (4 to 10 mm), using nylon mesh. Oocytes were exposed to the cryoprotectant composed of 40% (v/v) ethylene glycol, 18% (w/v) Ficoll-70, and 0.3 M sucrose in three stepwise dilutions. Thawing was conducted with a series of 0.5, 0.25 and 0.125 M sucrose dilutions in 20% fetal bovine serum. RESULTS:The cleavage (39.1% vs. 58.5%) and blastocyst rates (5.1% vs. 22.9%) were significantly lower for the vitrified oocytes. Follicle size had a significant impact on the development of embryos. Sires had significant effects on embryonic developmental rates. CONCLUSIONS:We conclude that differences in development exist due to follicle source and sire used for IVF after vitrification.

journal_name

J Assist Reprod Genet

journal_title

Journal of assisted reproduction and genetics

authors

Anchamparuthy VM,Dhali A,Lott WM,Pearson RE,Gwazdauskas FC

doi

10.1007/s10815-009-9362-2

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

613-9

issue

11-12

eissn

1058-0468

issn

1573-7330

journal_volume

26

pub_type

杂志文章

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