The accumulation of vitrified oocytes is a strategy to increase the number of euploid available blastocysts for transfer after preimplantation genetic testing.

Abstract:

PURPOSE:In a preimplantation genetic diagnosis for aneuploidy (PGD-A) program, the more embryos available for biopsy, consequently increases the chances of obtaining euploid embryos to transfer. The aim was to increase the number of viable euploid blastocysts in patients undergoing PGD-A using fresh oocytes together with previously accumulated vitrified oocytes. METHODS:Sixty-nine patients with normal ovarian reserve underwent PGD-A for repeated implantation failure or recurrent pregnancy loss indication. After several cycles of ovarian stimulation, 591 accumulated vitrified oocytes and 463 fresh oocytes were micro-injected with the same partner's semen sample. PGD-A was completed on 134 blastocysts from vitrified/warmed oocytes and 130 blastocysts from fresh oocytes. RESULTS:A mean of 9.6% euploid blastocyst per micro-injected vitrified/warmed oocytes and 11.4% euploid blastocyst per micro-injected fresh oocyte were obtained (p > 0.05). The euploidy and aneuploidy rates were comparable in blastocysts obtained from micro-injected vitrified/warmed oocytes and fresh oocytes (42.5 versus 40.8% and 57.5 versus 59.2%, p > 0.05). Implantation rates of euploid blastocysts were comparable between the two sources of oocytes (56.0% from vitrified/warmed oocytes versus 60.9% from fresh oocytes, p > 0.05). CONCLUSIONS:Oocyte vitrification and warming do not generate aneuploidy in blastocysts. The number of viable euploid embryos for transfer can be increased by using accumulated vitrified oocytes together with fresh oocytes in ICSI. TRIAL REGISTRATION:NCT02820415 ClinicalTrials.gov.

journal_name

J Assist Reprod Genet

authors

Chamayou S,Sicali M,Alecci C,Ragolia C,Liprino A,Nibali D,Storaci G,Cardea A,Guglielmino A

doi

10.1007/s10815-016-0868-0

subject

Has Abstract

pub_date

2017-04-01 00:00:00

pages

479-486

issue

4

eissn

1058-0468

issn

1573-7330

pii

10.1007/s10815-016-0868-0

journal_volume

34

pub_type

杂志文章
  • The antral follicle count predicts the outcome of pregnancy in a controlled ovarian hyperstimulation/intrauterine insemination program.

    abstract:PURPOSE:Our purpose was to test whether age-related changes in antral follicle counts can predict the pregnancy outcome in the early follicular phase of a controlled ovarian hyperstimulation/intrauterine insemination (COH/IUI) program. METHODS:A selected group of 107 women (36 healthy women requesting child sex presel...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1023/a:1022518103368

    authors: Chang MY,Chiang CH,Chiu TH,Hsieh TT,Soong YK

    更新日期:1998-01-01 00:00:00

  • Associations between toxic metals in follicular fluid and in vitro fertilization (IVF) outcomes.

    abstract:PURPOSE:We previously reported associations between trace concentrations of Hg, Cd and Pb in blood and urine and reproductive outcomes for women undergoing in-vitro fertilization (IVF). Here we assess measurements in single follicular fluid (FF) specimens from 46 women as a presumably more relevant marker of dose for r...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-012-9882-z

    authors: Bloom MS,Kim K,Kruger PC,Parsons PJ,Arnason JG,Steuerwald AJ,Fujimoto VY

    更新日期:2012-12-01 00:00:00

  • Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression.

    abstract:PURPOSE:The aim of the present study was to determine whether supplementation of resveratrol, a stilbenoid antioxidant with therapeutic significance, influences goat (Capra hircus) oocyte maturation and subsequent embryonic development and expression of apoptosis and early embryonic development-related genes. METHODS:...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-013-0116-9

    authors: Mukherjee A,Malik H,Saha AP,Dubey A,Singhal DK,Boateng S,Saugandhika S,Kumar S,De S,Guha SK,Malakar D

    更新日期:2014-02-01 00:00:00

  • Meta-analysis of the association of AhR Arg554Lys, AhRR Pro185Ala, and ARNT Val189Val polymorphisms and endometriosis risk in Asians.

    abstract:PURPOSE:Several studies have suggested an association between the polymorphisms AhR Arg554Lys, AhRR Pro185Ala, and ARNT Val189Val and endometriosis, but results have been inconclusive. The aim of the present study was to assess these associations by meta-analysis. METHODS:Eligible literatures were retrieved from PubMe...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,meta分析

    doi:10.1007/s10815-015-0505-3

    authors: Zheng NN,Bi YP,Zheng Y,Zheng RH

    更新日期:2015-07-01 00:00:00

  • Effect of a sonographically diffusely enlarged uterus without distinct uterine masses on the outcome of in vitro fertilization-embryo transfer.

    abstract:PURPOSE:Our objective was to study the effect of a sonographically diffusely enlarged uterus without distinct uterine masses on the outcome of in vitro fertilization-embryo transfer (IVF-ET). METHODS:Nineteen primary infertility patients undergoing IVF-ET who had a sonographically diffusely enlarged uterus without dis...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1023/a:1020593930366

    authors: Chiang CH,Chang MY,Shiau CS,Hou HC,Hsieh TT,Soong YK

    更新日期:1999-08-01 00:00:00

  • Ovum donation--an overview.

    abstract::Ovum donation is now an integral part of the management of infertility, providing a solution for patients previously considered permanently infertile. The indications now include not only patients with premature ovarian failure, but also patients who are carriers of genetic disorders, patients with repeated failures i...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,评审

    doi:10.1007/BF01207729

    authors: Benshushan A,Schenker JG

    更新日期:1993-02-01 00:00:00

  • Body-mind-spirit intervention for IVF women.

    abstract:BACKGROUND:Literature supports the efficacy of psychosocial intervention for infertile women. An eastern body-mind-spirit group intervention has been developed to help infertile women in the Chinese population cope with the distress arising from IVF treatment. METHODS:The eastern body-mind-spirit group intervention ad...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-005-6418-9

    authors: Chan CH,Chan CL,Ng SM,Ng EH,Ho PC

    更新日期:2005-12-01 00:00:00

  • Derivation, culture and retinal pigment epithelial differentiation of human embryonic stem cells using human fibroblast feeder cells.

    abstract:PURPOSE:Retinal pigment epithelium cells derived from human embryonic stem cells (ESCs) could be useful for restoring retinal function in age-related macular degeneration. However the use of non-human feeder cells to support the growth of ESCs for clinical applications raises the concern of possible contamination becau...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-012-9802-2

    authors: Zhang YS,Lu ZY,Yu Y,Li XR,Li WB,Wang YN,Geng Y

    更新日期:2012-08-01 00:00:00

  • "One for Sorrow, Two for Joy?": American embryo transfer guideline recommendations, practices, and outcomes for gestational surrogate patients.

    abstract::In January 2016, Melissa Cook, a California gestational surrogate experiencing a multiple-birth pregnancy following the in vitro fertilization (IVF) transfer of three embryos comprised of donor eggs and sperm provided by the intended father, went to the media when the intended father requested that she undergo a fetal...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s10815-017-0885-7

    authors: White PM

    更新日期:2017-04-01 00:00:00

  • Reduced blastocyst formation in reduced culture volume.

    abstract:PURPOSE:The aim of this prospective sibling oocyte study was to evaluate whether reduced culture volume improves blastocyst formation. METHODS:Twenty-three patients with extended embryo culture until day 5 were selected for the study. After injection, 345 sibling oocytes were individually cultured in either 25 or 7 μl...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-015-0541-z

    authors: De Munck N,Santos-Ribeiro S,Mateizel I,Verheyen G

    更新日期:2015-09-01 00:00:00

  • Improvement of IVF outcome in poor responders by discontinuation of GnRH analogue during the gonadotropin stimulation phase--a function of improved embryo quality.

    abstract:PURPOSE:To assess the efficacy of a protocol involving the discontinuation of the GnRH analogue at the mid-phase of ovarian stimulation for IVF in patients with a previous poor response. METHODS:Prospective case-control evaluation compared with same patient's previous performance. Thirty-six patients enrolled in an IV...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 临床试验,杂志文章

    doi:10.1023/a:1009476411762

    authors: Schachter M,Friedler S,Raziel A,Strassburger D,Bern O,Ron-el R

    更新日期:2001-04-01 00:00:00

  • Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes.

    abstract:OBJECTIVE:The aim of this study was to assess the fertilizability of unfertilized aged human oocytes from failed in vitro fertilization (IVF) cycles using SUZI and ICSI. METHODS:A total of 363 oocytes which showed no fertilization after conventional IVF was subjected to assisted fertilization using SUZI or ICSI. The m...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 临床试验,杂志文章

    doi:10.1007/BF02211724

    authors: Tsirigotis M,Bennett V,Nicholson N,Khalifa Y,Hogewind G,Yazdani N,Craft I

    更新日期:1994-09-01 00:00:00

  • The association between the oocyte pool and aneuploidy: a comparative study of the reproductive potential of young and aged mice.

    abstract:PURPOSE:The present study examined the effect of aging on female reproductive potential. METHODS:Six-week-old and 9-month-old CD1 mice were referred to as the 'young' and 'aged' groups, respectively. Oocytes were collected after superovulation, and their viability were compared using parthenogenetic activation. The an...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-013-0160-5

    authors: Fu X,Cheng J,Hou Y,Zhu S

    更新日期:2014-03-01 00:00:00

  • Medical egg freezing: the importance of a patient-centered approach to fertility preservation.

    abstract:PURPOSE:This binational qualitative study of medical egg freezing (MEF) examined women's motivations and experiences, including their perceived needs for patient-centered care in the midst of fertility- and life-threatening diagnoses. METHODS:Forty-five women who had undertaken MEF were interviewed in the USA (33 wome...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-017-1081-5

    authors: Inhorn MC,Birenbaum-Carmeli D,Westphal LM,Doyle J,Gleicher N,Meirow D,Raanani H,Dirnfeld M,Patrizio P

    更新日期:2018-01-01 00:00:00

  • Transcription profile of the insulin-like growth factor signaling pathway during human ovarian follicular development.

    abstract:PURPOSE:The IGF signaling cascade exerts important regulatory functions in human ovarian folliculogenesis. The scope of this study was to evaluate the transcription profile of insulin-like growth factor (IGF) genes during human ovarian follicle development and to analyze follicle fluid levels of key IGF proteins. METH...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-019-01432-x

    authors: Bøtkjær JA,Pors SE,Petersen TS,Kristensen SG,Jeppesen JV,Oxvig C,Andersen CY

    更新日期:2019-05-01 00:00:00

  • Association between peak estradiol levels and ovarian torsion among symptomatic patients receiving gonadotropin treatment.

    abstract:PURPOSE:Ovarian torsion is a surgical emergency that can be clinically challenging to diagnose. Patients who have received assisted reproductive technologies (ART) are a subset of women with an increased risk for torsion. As the ART population continues to increase, there is a need to delineate risk factors for the dev...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-017-0901-y

    authors: Romanski PA,Melamed A,Elias KM,Stanic AK,Anchan RM

    更新日期:2017-05-01 00:00:00

  • HOXA-11 mediated dysregulation of matrix remodeling during implantation window in women with endometriosis.

    abstract:PURPOSE:To investigate the Homeobox genes HOXA-10 and HOXA-11 mediated endometrial molecular defects during implantation window in endometriosis-associated infertility cases. METHODS:Endometrial biopsies were obtained during implantation window from 31 infertile women with endometriosis (age < 35 years) and 26 age and...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-013-0088-9

    authors: Jana SK,Banerjee P,Mukherjee R,Chakravarty B,Chaudhury K

    更新日期:2013-11-01 00:00:00

  • Pre-implantation developmental potential from in vivo and in vitro matured mouse oocytes: a cytoskeletal perspective on oocyte quality.

    abstract:PURPOSE:In the present study, fertilization and developmental potential of mouse oocytes matured in different conditions were tested. The efficiency of in vitro fertilization (IVF), pre-implantation development and some important aspects of cytokinesis during early cleavages are discussed. METHODS:In vivo matured (IVO...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-014-0363-4

    authors: Sanfins A,Plancha CE,Albertini DF

    更新日期:2015-01-01 00:00:00

  • The impact of early testicular sperm extraction or cryopreservation on the outcome of intracytoplasmic sperm injection--a randomized controlled study.

    abstract:PURPOSE:To compare the outcome of sperm extraction 24 h before ovum pickup and on the day of oocyte retrieval. METHODS:A controlled study was performed to compare the outcome of 90 sperm extractions and in vitro sperm injection cycles performed in 54 patients. RESULTS:Available fresh sperm for the sperm injection pro...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1023/a:1024116326465

    authors: Dirnfeld L,Paz M,Yshai D,Calderon I,Lahav-Baratz S,Koifman M,Shiloh H,Abramovici H,Bider D

    更新日期:2003-06-01 00:00:00

  • Correlation of somatic cell steroid secretion and quality of generated oocytes after in-vitro stimulation of mouse follicles.

    abstract:PURPOSE:To test the possibility of follicular somatic cell steroidogenesis as a marker for quality of their embraced oocytes. METHODS:Mechanically isolated mouse preantral follicles were cultured and matured in-vitro (IVC/IVM) for study. RESULTS:During IVC/IVM, oogenesis occurred concomitantly with folliculogenesis i...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-006-9041-5

    authors: Liu HC,He Z,Rosenwaks Z

    更新日期:2006-04-01 00:00:00

  • Mutational analysis of IZUMO1R in women with fertilization failure and polyspermy after in vitro fertilization.

    abstract:PURPOSE:The etiology of fertilization failure and polyspermy during assisted reproductive technology (ART) remains elusive. The aim of this study was to determine whether mutations in the IZUMO1 receptor (IZUMO1R) gene, which is essential for mammalian fertilization, contribute to the pathogenesis of fertilization fail...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-017-1101-5

    authors: Yu M,Zhao H,Chen T,Tian Y,Li M,Wu K,Bian Y,Su S,Cao Y,Ning Y,Liu H,Chen ZJ

    更新日期:2018-03-01 00:00:00

  • Qualitative and morphometric analysis of the ultrastructure of human oocytes cryopreserved by two alternative slow cooling protocols.

    abstract:PURPOSE:To ascertain possible cell damage from cryopreservation, the ultrastructure of human oocytes cryopreserved by slow cooling was assessed. MATERIALS AND METHODS:Cryopreservation was performed through two protocols with one-step or two-step propanediol. Fresh control oocytes were examined for comparison. Samples ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-010-9394-7

    authors: Coticchio G,Borini A,Distratis V,Maione M,Scaravelli G,Bianchi V,Macchiarelli G,Nottola SA

    更新日期:2010-04-01 00:00:00

  • Can apoptosis and necrosis coexist in ejaculated human spermatozoa during in vitro semen bacterial infection?

    abstract:PURPOSE:To evaluate whether ejaculated human spermatozoa undergo complete apoptosis or necrosis during experimental semen bacterial infection in vitro. METHODS:Apoptotic markers, including mitochondrial transmembrane potential (ΔΨm), phosphatidylserine (PS) externalization, and DNA fragmentation, have been detected si...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-015-0462-x

    authors: Fraczek M,Hryhorowicz M,Gaczarzewicz D,Szumala-Kakol A,Kolanowski TJ,Beutin L,Kurpisz M

    更新日期:2015-05-01 00:00:00

  • Early timed follicular aspiration prevents severe ovarian hyperstimulation syndrome.

    abstract:PURPOSE:Early timed follicular aspiration (ETFA) of one ovary 10-12 hr after administration of chorionic gonadotropin (hCG) is an attempt to prevent severe ovarian hyperstimulation syndrome (OHSS). After the introduction of early timed follicular aspiration ETFA of one ovary in IVF/ET cycles at high risk for severe OHS...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 临床试验,杂志文章

    doi:10.1007/BF02070139

    authors: Tomazevic T,Meden-Vrtovec H

    更新日期:1996-04-01 00:00:00

  • Maternal physical activity and sedentary behaviour before and during in vitro fertilization treatment: a longitudinal study exploring the associations with controlled ovarian stimulation and pregnancy outcomes.

    abstract:PURPOSE:To evaluate the association of objectively measured physical activity (PA) and sedentary behaviour before and during in vitro fertilization (IVF) with controlled ovarian stimulation (COS) and pregnancy outcomes. METHODS:This longitudinal study involved 107 infertile women undergoing IVF treatment. PA and seden...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-020-01864-w

    authors: Sõritsa D,Mäestu E,Nuut M,Mäestu J,Migueles JH,Läänelaid S,Ehrenberg A,Sekavin A,Sõritsa A,Salumets A,Ortega FB,Altmäe S

    更新日期:2020-08-01 00:00:00

  • Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation.

    abstract:PURPOSE:The main drawback of ovarian cryopreservation followed by transplantation is that a large proportion of follicles are lost after transplantation. Thus, effects of erythropoietin (EPO) and desialylated EPO administration on the frozen-thawed canine ovarian xenotransplantation were examined. METHODS:The protecti...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-008-9271-9

    authors: Suzuki H,Ishijima T,Maruyama S,Yanagimoto Ueta Y,Abe Y,Saitoh H

    更新日期:2008-11-01 00:00:00

  • Embryoscopy and karyotype findings of repeated miscarriages in recurrent pregnancy loss and spontaneous pregnancy loss.

    abstract:PURPOSE:The aim of the study was to assess cytogenetic and embryoscopic characteristics in subsequent miscarriages of spontaneous pregnancy losses (SPL) and recurrent pregnancy losses (RPL). METHODS:A retrospective cohort of 75 women was affected by repeated pregnancy loss. Of those, 34 had SPL, 24 primary RPL, and 17...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-018-1226-1

    authors: Feichtinger M,Reiner A,Hartmann B,Philipp T

    更新日期:2018-08-01 00:00:00

  • Interovarian differences in levels of cotinine, a major metabolite of nicotine, in women undergoing IVF who are exposed to cigarette smoke.

    abstract:PURPOSE:Our purpose was to determine whether there is variation in levels of follicular fluid (FF) cotinine between the two ovaries of women undergoing IVF-ET who are exposed to cigarette smoke. METHODS:In 61 women, there were two to four determinations of FF continine levels for each of two follicles, one from each o...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/BF02766834

    authors: Zenzes MT,Reed TE

    更新日期:1998-02-01 00:00:00

  • Unbalanced X;9 translocation in an infertile male with de novo duplication Xp22.31p22.33.

    abstract:PURPOSE:Male carriers of an X-autosome translocation are generally infertile, regardless of the position of the breakpoint on the X chromosome while the pathogenicity of Xp22.3 subtelomeric duplications is under debate. To shed light into this controversy, we present a rare case, of an azoospermic male with no other si...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-019-01405-0

    authors: Roumelioti FM,Louizou E,Karras S,Neroutsou R,Velissariou V,Gagos S

    更新日期:2019-04-01 00:00:00

  • Effects of aging on the male reproductive system.

    abstract::The study aims to discuss the effects of aging on the male reproductive system. A systematic review was performed using PubMed from 1980 to 2014. Aging is a natural process comprising of irreversible changes due to a myriad of endogenous and environmental factors at the level of all organs and systems. In modern life,...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s10815-016-0663-y

    authors: Gunes S,Hekim GN,Arslan MA,Asci R

    更新日期:2016-04-01 00:00:00