Abstract:
:Hepatocyte growth factor (HGF)-induced tubulogenesis has been demonstrated with renal epithelial cell lines grown in collagen gels but not with primary cultured renal proximal tubular epithelial cells (RPTEs). We show that HGF selectively induces proliferation and branching morphogenesis of primary cultured rat RPTEs. Additional growth factors including fibroblast growth factor (FGF)-1, epidermal growth factor (EGF), FGF-7, or insulin-like growth factor-1 (IGF-1) did not selectively induce tubulogenesis. However, when administered in combination, these factors initiated branching morphogenesis comparable to HGF alone and greatly augmented HGF-induced proliferation and branching. Microscopic analysis revealed that branching RPTEs were undergoing tubulogenesis and formed a polarized epithelium. TGF-beta1 blocked HGF- or growth factor cocktail (GFC; HGF, FGF-1, EGF, IGF-1)-induced proliferation and branching morphogenesis. Adding TGF-beta1 after GFC-induced tubulogenesis had occurred caused a progressive regression of the tubular structures, a response associated with an increase in apoptosis of the RPTEs. Primary cultured RPTEs are capable of undergoing HGF-induced tubulogenesis. Unlike cell lines, combinations of growth factors differentially augment the response.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Bowes RC 3rd,Lightfoot RT,Van De Water B,Stevens JLdoi
10.1002/(SICI)1097-4652(199907)180:1<81::AID-JCP9>keywords:
subject
Has Abstractpub_date
1999-07-01 00:00:00pages
81-90issue
1eissn
0021-9541issn
1097-4652pii
10.1002/(SICI)1097-4652(199907)180:1<81::AID-JCP9>journal_volume
180pub_type
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