In vivo antimicrobial activity of 2% chlorhexidine used as a root canal irrigating solution.

Abstract:

:The aim of the present study was to evaluate the in vivo antimicrobial activity of 2% chlorhexidine gluconate (FCFRP-USP) used as a root canal irrigating solution in teeth with pulp necrosis and radiographically visible chronic periapical reactions. Culture techniques and measurement of the inhibition zone were used. Twenty-two root canals of incisors and molars of 12 patients were used. After accessing the canal, the first root canal sample was collected with two sterile paper points that were transferred to a tube containing reduced transport fluid. The root canal was instrumented using chlorhexidine solution. A small sterile cotton pellet was placed at the root canal entrance, and the cavity was sealed with zinc oxide-eugenol cement. The canals were maintained empty for 48 h. Three sterile paper points were then introduced to absorb the root canal fluid (second sample). One paper point was placed on an agar plate inoculated with Micrococcus luteus ATCC 9341 and incubated for 24 h at 37 degrees C, and the other two were submitted to microbiological evaluation. Present in 10 cases at baseline, mutans streptococci was reduced by 100% at the second assessment. Treatment showed an efficiency of 77.78% for anaerobic microorganisms at the second assessment. These data suggest that chlorhexidine prevents microbial activity in vivo with residual effects in the root canal system up to 48 h.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Leonardo MR,Tanomaru Filho M,Silva LA,Nelson Filho P,Bonifácio KC,Ito IY

doi

10.1016/s0099-2399(99)80135-6

keywords:

subject

Has Abstract

pub_date

1999-03-01 00:00:00

pages

167-71

issue

3

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(99)80135-6

journal_volume

25

pub_type

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