Abstract:
:The recruitment of leukocytes to an area of injury or inflammation site is one of the most fundamental host defenses. Pulmonary tuberculosis is characterized by granulomatous inflammation with an extensive infiltration of mononuclear cells. In tuberculous pleurisy pleural mesothelial cells are exposed to mycobacteria in the pleural space. In this study we demonstrate that mouse pleural mesothelial cells (PMCs), when stimulated with BCG or IFN-gamma, produced MIP-1alpha and MCP-1 in vitro. IFN-gamma enhanced the BCG-mediated MIP-1alpha and MCP-1 expression in a concentration-dependent manner. The RT-PCR studies also confirmed that both BCG and IFN-gamma induce chemokine expression. IL-4 inhibited the BCG-mediated MIP-1alpha and MCP-1 expression in a concentration-dependent manner. The lower concentrations of IL-4 were ineffective; however, at higher concentrations, the inhibitory effect of IL-4 persisted for 24 h and decreased thereafter. BCG stimulation resulted in an increase of IFN-gamma and IL-4 receptors on PMCs. Our results demonstrate that Th1 and Th2 cytokines may regulate the C-C chemokine expression in PMCs and thus play a biologically important role in mononuclear cell recruitment to the pleural space.
journal_name
Am J Respir Crit Care Medauthors
Mohammed KA,Nasreen N,Ward MJ,Antony VBdoi
10.1164/ajrccm.159.5.9810011keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
1653-9issue
5 Pt 1eissn
1073-449Xissn
1535-4970journal_volume
159pub_type
杂志文章abstract:RATIONALE:Staphylococcus aureus is a major pathogen complicating postsurgical care. OBJECTIVES:To test the effect of sterile laparotomy (LAP) on pulmonary clearance of S. aureus in a murine model. METHODS:Control and LAP mice were infected intranasally with 10(8) cfu of S. aureus. Microbial clearance, pulmonary leuko...
journal_title:American journal of respiratory and critical care medicine
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更新日期:2007-11-01 00:00:00
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journal_title:American journal of respiratory and critical care medicine
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journal_title:American journal of respiratory and critical care medicine
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:American journal of respiratory and critical care medicine
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更新日期:2007-04-01 00:00:00
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journal_title:American journal of respiratory and critical care medicine
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更新日期:2006-06-15 00:00:00
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journal_title:American journal of respiratory and critical care medicine
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00