Mapping of lymphatic filariasis in loiasis areas: A new strategy shows no evidence for Wuchereria bancrofti endemicity in Cameroon.


BACKGROUND:Mapping of lymphatic filariasis (LF) caused by Wuchereria bancrofti largely relies on the detection of circulating antigen using ICT cards. Several studies have recently shown that this test can be cross-reactive with sera of subjects heavily infected with Loa loa and thus mapping results in loiasis endemic areas may be inaccurate. METHODOLOGY/PRINCIPAL FINDINGS:In order to develop an LF mapping strategy for areas with high loiasis prevalence, we collected day blood samples from 5,001 subjects residing in 50 villages that make up 6 health districts throughout Cameroon. Antigen testing using Filarial Test Strip (FTS, a novel platform that uses the same reagents as ICT) revealed an overall positivity rate of 1.1% and L. loa microfilaria (Mf) rates of up to 46%. Among the subjects with 0 to 8,000 Mf/ml in day blood, only 0.4% were FTS positive, while 22.2% of subjects with >8,000 Mf/ml were FTS positive. A Mf density of >8,200 Mf/ml was determined as the cut point at which positive FTS results should be excluded from the analysis. No FTS positive samples were also positive for W. bancrofti antibodies as measured by two different point of care tests that use the Wb123 antigen not found in L. loa. Night blood examination of the FTS positive subjects showed a high prevalence of L. loa Mf with densities up to 12,710 Mf/ml. No W. bancrofti Mf were identified, as confirmed by qPCR. Our results show that high loads of L. loa Mf in day blood are a reliable indicator of FTS positivity, and Wb123 rapid test proved to be relatively specific. CONCLUSIONS/SIGNIFICANCE:Our study provides a simple day blood-based algorithm for LF mapping in loiasis areas. The results indicate that many districts that were formerly classified as endemic for LF in Cameroon are non-endemic and do not require mass drug administration for elimination of LF.


PLoS Negl Trop Dis


Wanji S,Esum ME,Njouendou AJ,Mbeng AA,Chounna Ndongmo PW,Abong RA,Fru J,Fombad FF,Nchanji GT,Ngongeh G,Ngandjui NV,Enyong PI,Storey H,Curtis KC,Fischer K,Fauver JR,Lew D,Goss CW,Fischer PU




Has Abstract


2019-03-08 00:00:00














  • Backpack PCR: A point-of-collection diagnostic platform for the rapid detection of Brugia parasites in mosquitoes.

    abstract:BACKGROUND:Currently, molecular xenomonitoring efforts for lymphatic filariasis rely on PCR or real-time PCR-based detection of Brugia malayi, Brugia timori and Wuchereria bancrofti in mosquito vectors. Most commonly, extraction of DNA from mosquitoes is performed using silica column-based technologies. However, such e...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Zaky WI,Tomaino FR,Pilotte N,Laney SJ,Williams SA

    更新日期:2018-11-21 00:00:00

  • Zika virus like particles elicit protective antibodies in mice.

    abstract::Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as Zika fever. Since its recent emergence in 2014 in the American continent, ZIKV infection during pregnancy has been closely associated with a wide range of congenital abnormalities. To date, no vaccines or antivirals are publicl...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Salvo MA,Kingstad-Bakke B,Salas-Quinchucua C,Camacho E,Osorio JE

    更新日期:2018-02-05 00:00:00

  • A cluster of cases of severe fever with thrombocytopenia syndrome bunyavirus infection in China, 1996: A retrospective serological study.

    abstract:BACKGROUND:A cluster of eleven patients, including eight family members and three healthcare workers with fever and thrombocytopenia occurred in Yixing County, Jiangsu Province, China, from October to November 1996. However, the initial investigation failed to identify its etiology. Severe fever with thrombocytopenia s...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Hu J,Shi C,Li Z,Guo X,Qian Y,Tan W,Li X,Qi X,Su X,Zhou M,Wang H,Jiao Y,Bao C

    更新日期:2018-06-25 00:00:00

  • Increased miltefosine tolerance in clinical isolates of Leishmania donovani is associated with reduced drug accumulation, increased infectivity and resistance to oxidative stress.

    abstract:BACKGROUND:Miltefosine (MIL) is an oral antileishmanial drug used for treatment of visceral leishmaniasis (VL) in the Indian subcontinent. Recent reports indicate a significant decline in its efficacy with a high rate of relapse in VL as well as post kala-azar dermal leishmaniasis (PKDL). We investigated the parasitic ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Deep DK,Singh R,Bhandari V,Verma A,Sharma V,Wajid S,Sundar S,Ramesh V,Dujardin JC,Salotra P

    更新日期:2017-06-02 00:00:00

  • Human african trypanosomiasis diagnosis in first-line health services of endemic countries, a systematic review.

    abstract::While the incidence of Human African Trypanosomiasis (HAT) is decreasing, the control approach is shifting from active population screening by mobile teams to passive case detection in primary care centers. We conducted a systematic review of the literature between 1970 and 2011 to assess which diagnostic tools are mo...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Mitashi P,Hasker E,Lejon V,Kande V,Muyembe JJ,Lutumba P,Boelaert M

    更新日期:2012-01-01 00:00:00

  • Empiric deworming and CD4 count recovery in HIV-infected Ugandans initiating antiretroviral therapy.

    abstract:BACKGROUND:There is conflicting evidence on the immunologic benefit of treating helminth co-infections ("deworming") in HIV-infected individuals. Several studies have documented reduced viral load and increased CD4 count in antiretroviral therapy (ART) naïve individuals after deworming. However, there are a lack of dat...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Lankowski AJ,Tsai AC,Kanyesigye M,Bwana M,Haberer JE,Wenger M,Martin JN,Bangsberg DR,Hunt PW,Siedner MJ

    更新日期:2014-08-07 00:00:00

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Dabirian S,Taslimi Y,Zahedifard F,Gholami E,Doustdari F,Motamedirad M,Khatami S,Azadmanesh K,Nylen S,Rafati S

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  • Improved reliability of serological tools for the diagnosis of West Nile fever in horses within Europe.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Beck C,Lowenski S,Durand B,Bahuon C,Zientara S,Lecollinet S

    更新日期:2017-09-15 00:00:00

  • Flubendazole as a macrofilaricide: History and background.

    abstract::Benzimidazole anthelmintics have long been employed for the control of soil-transmitted helminth infections. Flubendazole (FBZ) was approved in 1980 for the treatment of gastrointestinal nematode infections in both veterinary and human medicine. It has also long been known that parenteral administration of FBZ can lea...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Geary TG,Mackenzie CD,Silber SA

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  • NADPH phagocyte oxidase knockout mice control Trypanosoma cruzi proliferation, but develop circulatory collapse and succumb to infection.

    abstract::(•)NO is considered to be a key macrophage-derived cytotoxic effector during Trypanosoma cruzi infection. On the other hand, the microbicidal properties of reactive oxygen species (ROS) are well recognized, but little importance has been attributed to them during in vivo infection with T. cruzi. In order to investigat...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Santiago HC,Gonzalez Lombana CZ,Macedo JP,Utsch L,Tafuri WL,Campagnole-Santos MJ,Alves RO,Alves-Filho JC,Romanha AJ,Cunha FQ,Teixeira MM,Radi R,Vieira LQ

    更新日期:2012-01-01 00:00:00

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    abstract:BACKGROUND:Antibody-detecting rapid diagnostic tests (RDTs) against rK39 are available to aid in the diagnosis of visceral leishmaniasis (VL). Although these rK39 RDTs have been developed, validated and approved for use with serum, they are universally performed using whole blood. It was therefore necessary to determin...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Matlashewski G,Das VN,Pandey K,Singh D,Das S,Ghosh AK,Pandey RN,Das P

    更新日期:2013-05-23 00:00:00

  • The cost-effectiveness of an eradication programme in the end game: Evidence from guinea worm disease.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fitzpatrick C,Sankara DP,Agua JF,Jonnalagedda L,Rumi F,Weiss A,Braden M,Ruiz-Tiben E,Kruse N,Braband K,Biswas G

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Valzania L,Martinson VG,Harrison RE,Boyd BM,Coon KL,Brown MR,Strand MR

    更新日期:2018-07-06 00:00:00

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Chakraborty C,Clayton C

    更新日期:2018-10-01 00:00:00

  • Comparing models for early warning systems of neglected tropical diseases.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Chaves LF,Pascual M

    更新日期:2007-10-22 00:00:00

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    pub_type: 杂志文章


    authors: Gallian P,Leparc-Goffart I,Richard P,Maire F,Flusin O,Djoudi R,Chiaroni J,Charrel R,Tiberghien P,de Lamballerie X

    更新日期:2017-01-12 00:00:00

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Graf FE,Ludin P,Wenzler T,Kaiser M,Brun R,Pyana PP,Büscher P,de Koning HP,Horn D,Mäser P

    更新日期:2013-10-10 00:00:00

  • Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


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  • Rodent-borne diseases and their public health importance in Iran.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Rabiee MH,Mahmoudi A,Siahsarvie R,Kryštufek B,Mostafavi E

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Chauffour A,Robert J,Veziris N,Aubry A,Pethe K,Jarlier V

    更新日期:2020-08-31 00:00:00

  • A replicating cytomegalovirus-based vaccine encoding a single Ebola virus nucleoprotein CTL epitope confers protection against Ebola virus.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Tsuda Y,Caposio P,Parkins CJ,Botto S,Messaoudi I,Cicin-Sain L,Feldmann H,Jarvis MA

    更新日期:2011-08-01 00:00:00

  • Body lice of homeless people reveal the presence of several emerging bacterial pathogens in northern Algeria.

    abstract:BACKGROUND:Human lice, Pediculus humanus, are obligate blood-sucking parasites. Body lice, Pediculus h. humanus, occur in two divergent mitochondrial clades (A and D) each exhibiting a particular geographic distribution. Currently, the body louse is recognized as the only vector for louse-borne diseases. In this study,...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Louni M,Mana N,Bitam I,Dahmani M,Parola P,Fenollar F,Raoult D,Mediannikov O

    更新日期:2018-04-17 00:00:00

  • Health-related impact on quality of life and coping strategies for chikungunya: A qualitative study in Curaçao.

    abstract:INTRODUCTION:Chikungunya is an emerging public health problem in tropical and subtropical regions, due to ongoing transmission and its incapacitating acute disease phase, and chronic sequelae. The disease is responsible for a major impact on Health Related Quality of Life (HRQoL), which may last several years. To our k...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Elsinga J,Grobusch MP,Tami A,Gerstenbluth I,Bailey A

    更新日期:2017-10-09 00:00:00

  • A new model for management of mycetoma in the Sudan.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fahal A,Mahgoub el S,El Hassan AM,Abdel-Rahman ME,Alshambaty Y,Hashim A,Hago A,Zijlstra EE

    更新日期:2014-10-30 00:00:00

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Nyakarahuka L,Skjerve E,Nabadda D,Sitali DC,Mumba C,Mwiine FN,Lutwama JJ,Balinandi S,Shoemaker T,Kankya C

    更新日期:2017-09-11 00:00:00

  • Changes over Time in IgE Sensitization to Allergens of the Fish Parasite Anisakis spp.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Carballeda-Sangiao N,Rodríguez-Mahillo AI,Careche M,Navas A,Moneo I,González-Muñoz M

    更新日期:2016-07-22 00:00:00

  • Biodegradable nanocarriers coated with polymyxin B: Evaluation of leishmanicidal and antibacterial potential.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Souza Ribeiro Costa J,Medeiros M,Yamashiro-Kanashiro EH,Rocha MC,Cotrim PC,Stephano MA,Lancellotti M,Tavares GD,Oliveira-Nascimento L

    更新日期:2019-05-01 00:00:00

  • Diagnostic accuracy of direct agglutination test, rK39 ELISA and six rapid diagnostic tests among visceral leishmaniasis patients with and without HIV coinfection in Ethiopia.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Kassa M,Abdellati S,Cnops L,Bremer Hinckel BC,Yeshanew A,Hailemichael W,Vogt F,Adriaensen W,Mertens P,Diro E,van Griensven J,Van den Bossche D

    更新日期:2020-12-31 00:00:00

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Standley CJ,Graeden E,Kerr J,Sorrell EM,Katz R

    更新日期:2018-04-12 00:00:00

  • Major risk factors and histopathological profile of treatment failure, relapse and chronic patients with anthroponotic cutaneous leishmaniasis: A prospective case-control study on treatment outcome and their medical importance.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Bamorovat M,Sharifi I,Dabiri S,Shamsi Meymandi S,Karamoozian A,Amiri R,Heshmatkhah A,Borhani Zarandi M,Aflatoonian MR,Sharifi F,Kheirandish R,Hassanzadeh S

    更新日期:2021-01-28 00:00:00