The Use of TB-Specific Antigen/Phytohemagglutinin Ratio for Diagnosis and Treatment Monitoring of Extrapulmonary Tuberculosis.

Abstract:

:Extrapulmonary tuberculosis (EPTB) has become more common in recent years; however, the diagnosis of EPTB remains a challenge. In this study, we analyzed the performance of the ratio of TB-specific antigen (TBAg) to phytohemagglutinin (PHA) (TBAg/PHA ratio) in T-SPOT.TB (T-SPOT) assay for diagnosis and treatment monitoring of EPTB. Between 2012 and 2017, 734 EPTB patients were diagnosed and recruited from Tongji hospital, and 1,137 suspected EPTB patients who had other diagnoses were recruited as non-EPTB controls. To validate the study, another small group of EPTB patients and non-EPTB controls were recruited from Sino-French New City Branch of Tongji Hospital. The positive rate of peripheral blood T-SPOT in EPTB and non-EPTB were 88.15 and 32.28%. In T-SPOT positive patients, the direct T-SPOT results had limited value in distinguishing these two conditions. A further calculation of the TBAg/PHA ratio of T-SPOT showed improved performance in each form of EPTB. If using 0.20 as the threshold value of the TBAg/PHA ratio, the pooled sensitivity and specificity were 70.79 and 91.55% in distinguishing EPTB from non-EPTB. The validation results showed a better performance of the TBAg/PHA ratio in distinguishing these two conditions, with a sensitivity and specificity of 81.82 and 97.56%, respectively. Comparing with directly using T-SPOT results, the TBAg/PHA ratio was less affected by immunosuppression. Furthermore, PHA value reflected immunosuppression and could help to judge the credibility of T-SPOT results in EPTB patients with different immune status. The TBAg/PHA ratio was significantly decreased during anti-tuberculosis (TB) treatment, which suggests that it can also be used to monitor therapeutic efficacy. These data provide new insights into the role of T-SPOT assay in TB disease, and the TBAg/PHA ratio might be a useful tool for diagnosis and treatment monitoring of EPTB.

journal_name

Front Immunol

journal_title

Frontiers in immunology

authors

Wang F,Yu J,Zhou Y,Luo Y,Wu S,Huang M,Yin B,Huang J,Mao L,Sun Z

doi

10.3389/fimmu.2018.01047

subject

Has Abstract

pub_date

2018-05-14 00:00:00

pages

1047

issn

1664-3224

journal_volume

9

pub_type

杂志文章
  • CD70 Deficiency Associated With Chronic Epstein-Barr Virus Infection, Recurrent Airway Infections and Severe Gingivitis in a 24-Year-Old Woman.

    abstract::Most of the few patients with homozygous CD70 deficiency described to date suffered from EBV-related malignancies in early childhood. We present a woman with CD70 deficiency diagnosed in adulthood. She presented in childhood with recurrent airway infections due to encapsulated bacteria, herpes zoster and a fulminant E...

    journal_title:Frontiers in immunology

    pub_type:

    doi:10.3389/fimmu.2020.01593

    authors: Krüger R,Martin E,Dmytrus J,Feiterna-Sperling C,Meisel C,Unterwalder N,Kölsch U,Wahn V,Hofmann J,Korn P,Latour S,Boztug K,von Bernuth H

    更新日期:2020-08-04 00:00:00

  • Single or Combined Dietary Supply of Psidium guajava and Phyllanthus amarus Extracts Differentially Modulate Immune Responses and Liver Proteome in Striped Catfish (Pangasianodon hyphophthalmus).

    abstract::Guava Psidium guajava L (Pg) and bhumi amla Phyllanthus amarus Schum. et Thonn (Pa) are well-known plants in traditional medicine. However, the capacity of these plants for improving the immune system of aquatic species has received less attention so far. This study aimed to investigate the effects of single supply or...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.00797

    authors: Nhu TQ,Bich Hang BT,Cornet V,Oger M,Bach LT,Anh Dao NL,Thanh Huong DT,Quetin-Leclercq J,Scippo ML,Phuong NT,Kestemont P

    更新日期:2020-05-05 00:00:00

  • Arid5a Regulation and the Roles of Arid5a in the Inflammatory Response and Disease.

    abstract::Abnormal gene expression patterns underlie many diseases that represent major public health concerns and robust therapeutic challenges. Posttranscriptional gene regulation by RNA-binding proteins (RBPs) is well-recognized, and the biological functions of RBPs have been implicated in many diseases, such as autoimmune d...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2019.02790

    authors: Nyati KK,Agarwal RG,Sharma P,Kishimoto T

    更新日期:2019-12-05 00:00:00

  • Reflections on HLA Epitope-Based Matching for Transplantation.

    abstract::HLA antibodies are primary causes of transplant rejection; they recognize epitopes that can be structurally defined by eplets. There are many reviews about HLA epitope-based matching in transplantation. This article describes some personal reflections about epitopes including a historical perspective of HLA typing at ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2016.00469

    authors: Duquesnoy RJ

    更新日期:2016-11-28 00:00:00

  • Tumor-Specific Antibody, Cetuximab, Enhances the In Situ Vaccine Effect of Radiation in Immunologically Cold Head and Neck Squamous Cell Carcinoma.

    abstract::In head and neck squamous cell carcinoma (HNSCC) tumors that over-expresses huEGFR, the anti-EGFR antibody, cetuximab, antagonizes tumor cell viability and sensitizes to radiation therapy. However, the immunologic interactions between cetuximab and radiation therapy are not well understood. We transduced two syngeneic...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.591139

    authors: Jin WJ,Erbe AK,Schwarz CN,Jaquish AA,Anderson BR,Sriramaneni RN,Jagodinsky JC,Bates AM,Clark PA,Le T,Lan KH,Chen Y,Kim K,Morris ZS

    更新日期:2020-11-12 00:00:00

  • The Signature Amino Acid Residue Serine 31 of HIV-1C Tat Potentiates an Activated Phenotype in Endothelial Cells.

    abstract::The natural cysteine to serine variation at position 31 of Tat in HIV-1C disrupts the dicysteine motif attenuating the chemokine function of Tat. We ask if there exists a trade-off in terms of a gain of function for HIV-1C Tat due to this natural variation. We constructed two Tat-expression vectors encoding Tat protei...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.529614

    authors: Menon M,Budhwar R,Shukla RN,Bankar K,Vasudevan M,Ranga U

    更新日期:2020-09-25 00:00:00

  • Phenotypical Characterization of Spleen Remodeling in Murine Experimental Visceral Leishmaniasis.

    abstract::Background: Visceral leishmaniasis (VL) is caused by Leishmania infantum or L. donovani infection. One of the main problems related to this disease is the emergence of severe clinical forms with a lethality of 5-20%, even while under specific treatment. In humans and other species susceptible to fatal VL, such as dogs...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.00653

    authors: de Melo CVB,Hermida MD,Mesquita BR,Fontes JLM,Koning JJ,Solcà MDS,Benevides BB,Mota GBS,Freitas LAR,Mebius RE,Dos-Santos WLC

    更新日期:2020-04-15 00:00:00

  • Excess of Mortality in Adults and Elderly and Circulation of Subtypes of Influenza Virus in Southern Brazil.

    abstract:Purpose:In the elderly population, the influenza infection and its clinical complications are important causes of hospitalization and death, particularly, in longer-lived age. The objective of this study is to analyze the impact of influenza virus circulation on mortality in the elderly and adults, in years with differ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2017.01903

    authors: Freitas ARR,Donalisio MR

    更新日期:2018-01-08 00:00:00

  • Erratum: Defining Mononuclear Phagocyte Subset Homology across Several Distant Warm-Blooded Vertebrates through Comparative Transcriptomics.

    abstract::[This corrects the article on p. 299 in vol. 6, PMID: 26150816.]. ...

    journal_title:Frontiers in immunology

    pub_type: 已发布勘误

    doi:10.3389/fimmu.2016.00143

    authors: Frontiers Production Office.

    更新日期:2016-04-28 00:00:00

  • Alum/Toll-Like Receptor 7 Adjuvant Enhances the Expansion of Memory B Cell Compartment Within the Draining Lymph Node.

    abstract::Vaccination is one of the most cost-effective health interventions and, with the exception of water sanitization, no other action has had such a major effect in mortality reduction. Combined with other approaches, such as clean water, better hygiene, and health education, vaccination contributed to prevent millions of...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2018.00641

    authors: Vo HTM,Baudner BC,Sammicheli S,Iannacone M,D'Oro U,Piccioli D

    更新日期:2018-04-09 00:00:00

  • Corrigendum: Blockade of IL-33R/ST2 Signaling Attenuates Toxoplasma gondii Ileitis Depending on IL-22 Expression.

    abstract::[This corrects the article DOI: 10.3389/fimmu.2019.00702.]. ...

    journal_title:Frontiers in immunology

    pub_type: 已发布勘误

    doi:10.3389/fimmu.2020.592865

    authors: Ryffel B,Huang F,Robinet P,Panek C,Couillin I,Erard F,Piotet J,Le Bert M,Mackowiak C,Torres Arias M,Dimier-Poisson I,Zheng SG

    更新日期:2020-10-28 00:00:00

  • Natural Killer Cells in Myeloid Malignancies: Immune Surveillance, NK Cell Dysfunction, and Pharmacological Opportunities to Bolster the Endogenous NK Cells.

    abstract::Natural killer (NK) cells are large granular lymphocytes involved in our defense against certain virus-infected and malignant cells. In contrast to T cells, NK cells elicit rapid anti-tumor responses based on signals from activating and inhibitory cell surface receptors. They also lyse target cells via antibody-depend...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2019.02357

    authors: Carlsten M,Järås M

    更新日期:2019-10-11 00:00:00

  • iRAGu: A Novel Inducible and Reversible Mouse Model for Ubiquitous Recombinase Activity.

    abstract::Developing lymphocytes express the recombination activating genes (RAGs) 1 and 2 products that form a site specific recombinase complex (RAG), introducing double strand DNA breaks (DSBs) at recombination signal sequences (RSSs) flanking the V, D, and J gene segments in the antigen receptor loci. The subsequent steps i...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2017.01525

    authors: Bonnet M,Sarmento LM,Martins AC,Sobral D,Silva J,Demengeot J

    更新日期:2017-11-10 00:00:00

  • Effective Targeting of TAG72+ Peritoneal Ovarian Tumors via Regional Delivery of CAR-Engineered T Cells.

    abstract::Impressive clinical efficacy of chimeric antigen receptor (CAR)-engineered T cell therapy for hematological malignancies have prompted significant efforts in achieving similar responses in solid tumors. The lack of truly restricted and uniform expression of tumor-associated antigens, as well as limited T cell persiste...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2018.02268

    authors: Murad JP,Kozlowska AK,Lee HJ,Ramamurthy M,Chang WC,Yazaki P,Colcher D,Shively J,Cristea M,Forman SJ,Priceman SJ

    更新日期:2018-11-19 00:00:00

  • Fli1 Downregulation in Scleroderma Myeloid Cells Has Profibrotic and Proinflammatory Effects.

    abstract::Scleroderma (SSc) is an autoimmune connective tissue disease characterized by immune dysregulation, vasculopathy, and fibrosis. We have previously demonstrated that low Fli1 expression in SSc fibroblasts and endothelial cells plays an important role in SSc pathogenesis. Cells of myeloid and lymphoid origin also expres...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.00800

    authors: Bujor AM,El Adili F,Parvez A,Marden G,Trojanowska M

    更新日期:2020-05-19 00:00:00

  • Bidirectional Action of Cenicriviroc, a CCR2/CCR5 Antagonist, Results in Alleviation of Pain-Related Behaviors and Potentiation of Opioid Analgesia in Rats With Peripheral Neuropathy.

    abstract::Clinical management of neuropathic pain is unsatisfactory, mainly due to its resistance to the effects of available analgesics, including opioids. Converging evidence indicates the functional interactions between chemokine and opioid receptors and their influence on nociceptive processes. Recent studies highlight that...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.615327

    authors: Kwiatkowski K,Pawlik K,Ciapała K,Piotrowska A,Makuch W,Mika J

    更新日期:2020-12-21 00:00:00

  • Molecular Mechanisms That Underlie the Dynamic Adaptation of Innate Monocyte Memory to Varying Stimulant Strength of TLR Ligands.

    abstract::In adaptation to rising stimulant strength, innate monocytes can be dynamically programed to preferentially express either pro- or anti-inflammatory mediators. Such dynamic innate adaptation or programing may bear profound relevance in host health and disease. However, molecular mechanisms that govern innate adaptatio...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2016.00497

    authors: Yuan R,Geng S,Li L

    更新日期:2016-11-10 00:00:00

  • Mast Cells and MCPT4 Chymase Promote Renal Impairment after Partial Ureteral Obstruction.

    abstract::Obstructive nephropathy constitutes a major cause of pediatric renal progressive disease. The mechanisms leading to disease progression are still poorly understood. Kidney fibrotic lesions are reproduced using a model of partial unilateral ureteral obstruction (pUUO) in newborn mice. Based on data showing significant ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2017.00450

    authors: Pons M,Ali L,Beghdadi W,Danelli L,Alison M,Madjène LC,Calvo J,Claver J,Vibhushan S,Åbrink M,Pejler G,Poli-Mérol ML,Peuchmaur M,El Ghoneimi A,Blank U

    更新日期:2017-05-04 00:00:00

  • Immunization route dictates cross-priming efficiency and impacts the optimal timing of adjuvant delivery.

    abstract::Delivery of cell-associated antigen represents an important strategy for vaccination. While many experimental models have been developed in order to define the critical parameters for efficient cross-priming, few have utilized quantitative methods that permit the study of the endogenous repertoire. Comparing different...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2011.00071

    authors: Bouvier I,Jusforgues-Saklani H,Lim A,Lemaître F,Lemercier B,Auriau C,Nicola MA,Leroy S,Law HK,Bandeira A,Moon JJ,Bousso P,Albert ML

    更新日期:2011-12-08 00:00:00

  • Metabolic regulation of regulatory T cell development and function.

    abstract::It is now well established that the effector T cell (Teff) response is regulated by a series of metabolic switches. Quiescent T cells predominantly require adenosine triphosphate-generating processes, whereas proliferating Teff require high metabolic flux through growth-promoting pathways, such as glycolysis. Pathways...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2014.00590

    authors: Coe DJ,Kishore M,Marelli-Berg F

    更新日期:2014-11-18 00:00:00

  • Quantity and Quality of Inhaled Dose Predicts Immunopathology in Tuberculosis.

    abstract::Experimental animal models of tuberculosis (TB) have convincingly demonstrated that inhaled dose predicts immunopathology and survival. In contrast, the importance of inhaled dose has generally not been appreciated in TB epidemiology, clinical science, or the practice of TB control. Infectiousness of TB patients has t...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2015.00313

    authors: Fennelly KP,Jones-López EC

    更新日期:2015-06-29 00:00:00

  • Protein Kinase C-θ (PKC-θ) in Natural Killer Cell Function and Anti-Tumor Immunity.

    abstract::The protein kinase C-θ (PKCθ), which is essential for T cell function and survival, is also required for efficient anti-tumor immune surveillance. Natural killer (NK) cells, which express PKCθ, play a prominent role in this process, mainly by elimination of tumor cells with reduced or absent major histocompatibility c...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2012.00187

    authors: Anel A,Aguiló JI,Catalán E,Garaude J,Rathore MG,Pardo J,Villalba M

    更新日期:2012-07-05 00:00:00

  • Serum Amyloid A Stimulates Vascular and Renal Dysfunction in Apolipoprotein E-Deficient Mice Fed a Normal Chow Diet.

    abstract::Elevated serum amyloid A (SAA) levels may promote endothelial dysfunction, which is linked to cardiovascular and renal pathologies. We investigated the effect of SAA on vascular and renal function in apolipoprotein E-deficient (ApoE-/-) mice. Male ApoE-/- mice received vehicle (control), low-level lipopolysaccharide (...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2019.00380

    authors: Chami B,Hossain F,Hambly TW,Cai X,Aran R,Fong G,Vellajo A,Martin NJJ,Wang X,Dennis JM,Sharma A,Shihata WA,Chin-Dusting JPF,de Haan JB,Sharland A,Geczy CL,Freedman B,Witting PK

    更新日期:2019-03-07 00:00:00

  • iCa2+ Flux, ROS and IL-10 Determines Cytotoxic, and Suppressor T Cell Functions in Chronic Human Viral Infections.

    abstract::Exhaustion of CD8+ T cells and increased IL-10 production is well-known in chronic viral infections but mechanisms leading to loss of their cytotoxic capabilities and consequent exhaustion remain unclear. Exhausted CD8+T cells also called T suppressors are highly immune suppressive with altered T cell receptor signali...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.00083

    authors: Mohanty S,Barik P,Debata N,Nagarajan P,Devadas S

    更新日期:2020-03-06 00:00:00

  • Considerations in Preparative Regimen Selection to Minimize Rejection in Pediatric Hematopoietic Transplantation in Non-Malignant Diseases.

    abstract::The variables that influence the selection of a preparative regimen for a pediatric hematopoietic stem cell transplant procedure encompasses many issues. When one considers this procedure for non-malignant diseases, components in a preparative regimen that were historically developed to reduce malignant tumor burden m...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2020.567423

    authors: Hayashi RJ

    更新日期:2020-10-19 00:00:00

  • Oxidized Hemoglobin Is Antigenic and Immunogenic in Lupus.

    abstract::Hemolysis-associated anemia is characteristic of diseases such as atherosclerosis, lupus, malaria, and leishmaniasis; the toxic effects of free hemoglobin (Hb) have been extensively described. This study was based on the premise that release of this sequestered, inflammatory molecule can result in deleterious immunolo...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2017.00732

    authors: Jain S,Bose A,Bastia B,Sharma H,Sachdeva R,Jain AK,Pal R

    更新日期:2017-06-26 00:00:00

  • Humoral Immunity vs. Salmonella.

    abstract::In primary infection with Salmonella, it has been reported-without consideration of Salmonella's functions-that humoral immunity plays no role in the clearance of bacteria. In fact, Salmonella targets and suppresses several aspects of humoral immunity, including B cell lymphopoiesis, B cell activation, and IgG product...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2019.03155

    authors: Takaya A,Yamamoto T,Tokoyoda K

    更新日期:2020-01-21 00:00:00

  • The NF-κB Signaling Pathway, the Microbiota, and Gastrointestinal Tumorigenesis: Recent Advances.

    abstract::Gastrointestinal (GI) cancers, especially gastric cancer and colorectal cancer (CRC), represent a major global health burden. A large population of microorganisms residing in the GI tract regulate physiological processes, such as the immune response, metabolic balance, and homeostasis. Accumulating evidence has reveal...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2020.01387

    authors: Peng C,Ouyang Y,Lu N,Li N

    更新日期:2020-06-30 00:00:00

  • Vaccine-Induced Antibodies Mediate Higher Antibody-Dependent Cellular Cytotoxicity After Interleukin-15 Pretreatment of Natural Killer Effector Cells.

    abstract::The secondary analyses for correlates of risk of infection in the RV144 HIV-1 vaccine trial implicated vaccine-induced antibody-dependent cellular cytotoxicity (ADCC) responses in the observed protection, highlighting the importance of assessing such responses in ongoing and future HIV-1 vaccine trials. However, in vi...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,随机对照试验

    doi:10.3389/fimmu.2019.02741

    authors: Fisher L,Zinter M,Stanfield-Oakley S,Carpp LN,Edwards RW,Denny T,Moodie Z,Laher F,Bekker LG,McElrath MJ,Gilbert PB,Corey L,Tomaras G,Pollara J,Ferrari G

    更新日期:2019-11-27 00:00:00

  • Hematopoietic Stem Cell Transplantation in an Infant with Immunodeficiency, Centromeric Instability, and Facial Anomaly Syndrome.

    abstract::Immunodeficiency, centromeric instability, and facial anomaly (ICF) syndrome is a rare autosomal recessive genetic condition with severe immunodeficiency, which leads to lethal infections if not recognized and treated in early childhood. Up-to-date treatment regimens consist of prophylactic and supportive treatment of...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2017.00773

    authors: Gössling KL,Schipp C,Fischer U,Babor F,Koch G,Schuster FR,Dietzel-Dahmen J,Wieczorek D,Borkhardt A,Meisel R,Kuhlen M

    更新日期:2017-06-30 00:00:00