The Use of TB-Specific Antigen/Phytohemagglutinin Ratio for Diagnosis and Treatment Monitoring of Extrapulmonary Tuberculosis.

Abstract:

:Extrapulmonary tuberculosis (EPTB) has become more common in recent years; however, the diagnosis of EPTB remains a challenge. In this study, we analyzed the performance of the ratio of TB-specific antigen (TBAg) to phytohemagglutinin (PHA) (TBAg/PHA ratio) in T-SPOT.TB (T-SPOT) assay for diagnosis and treatment monitoring of EPTB. Between 2012 and 2017, 734 EPTB patients were diagnosed and recruited from Tongji hospital, and 1,137 suspected EPTB patients who had other diagnoses were recruited as non-EPTB controls. To validate the study, another small group of EPTB patients and non-EPTB controls were recruited from Sino-French New City Branch of Tongji Hospital. The positive rate of peripheral blood T-SPOT in EPTB and non-EPTB were 88.15 and 32.28%. In T-SPOT positive patients, the direct T-SPOT results had limited value in distinguishing these two conditions. A further calculation of the TBAg/PHA ratio of T-SPOT showed improved performance in each form of EPTB. If using 0.20 as the threshold value of the TBAg/PHA ratio, the pooled sensitivity and specificity were 70.79 and 91.55% in distinguishing EPTB from non-EPTB. The validation results showed a better performance of the TBAg/PHA ratio in distinguishing these two conditions, with a sensitivity and specificity of 81.82 and 97.56%, respectively. Comparing with directly using T-SPOT results, the TBAg/PHA ratio was less affected by immunosuppression. Furthermore, PHA value reflected immunosuppression and could help to judge the credibility of T-SPOT results in EPTB patients with different immune status. The TBAg/PHA ratio was significantly decreased during anti-tuberculosis (TB) treatment, which suggests that it can also be used to monitor therapeutic efficacy. These data provide new insights into the role of T-SPOT assay in TB disease, and the TBAg/PHA ratio might be a useful tool for diagnosis and treatment monitoring of EPTB.

journal_name

Front Immunol

journal_title

Frontiers in immunology

authors

Wang F,Yu J,Zhou Y,Luo Y,Wu S,Huang M,Yin B,Huang J,Mao L,Sun Z

doi

10.3389/fimmu.2018.01047

subject

Has Abstract

pub_date

2018-05-14 00:00:00

pages

1047

issn

1664-3224

journal_volume

9

pub_type

杂志文章
  • TLR7/TLR9- and B Cell Receptor-Signaling Crosstalk: Promotion of Potentially Dangerous B Cells.

    abstract::B cells are capable of receptor-mediated responses to foreign antigens. Recognition of microbial-derived nucleic acid (NA) by toll-like receptors (TLRs) 7 and 9 in B cells has been substantiated. Endogenous NA released from damaged or dying cells can also be immunogenic in certain contexts and can incite aberrant acti...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2017.00775

    authors: Suthers AN,Sarantopoulos S

    更新日期:2017-07-13 00:00:00

  • IL-10 and IL-22 in Mucosal Immunity: Driving Protection and Pathology.

    abstract::The barrier surfaces of the gastrointestinal tract are in constant contact with various microorganisms. Cytokines orchestrate the mucosal adaptive and innate immune cells in the defense against pathogens. IL-10 and IL-22 are the best studied members of the IL-10 family and play essential roles in maintaining mucosal h...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2020.01315

    authors: Wei HX,Wang B,Li B

    更新日期:2020-06-26 00:00:00

  • Trypanosoma cruzi Exploits Wnt Signaling Pathway to Promote Its Intracellular Replication in Macrophages.

    abstract::During the acute phase of Trypanosoma cruzi infection, macrophages can act as host cells for the parasites as well as effector cells in the early anti-parasitic immune response. Thus, the targeting of specific signaling pathways could modulate macrophages response to restrict parasite replication and instruct an appro...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2018.00859

    authors: Volpini X,Ambrosio LF,Fozzatti L,Insfran C,Stempin CC,Cervi L,Motran CC

    更新日期:2018-04-23 00:00:00

  • Mesenchymal cell differentiation during lymph node organogenesis.

    abstract::Secondary lymphoid tissues such as lymph nodes are essential for the interactions between antigen presenting cells and lymphocytes that result in adaptive immune responses that protect the host against invading pathogens. The specialized architecture of these organs facilitates the cognate interactions between antigen...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2012.00381

    authors: Brendolan A,Caamaño JH

    更新日期:2012-12-14 00:00:00

  • Cyclic Peptide [R4W4] in Improving the Ability of First-Line Antibiotics to Inhibit Mycobacterium tuberculosis Inside in vitro Human Granulomas.

    abstract::Tuberculosis (TB) is currently one of the leading causes of global mortality. Medical non-compliance due to the length of the treatment and antibiotic side effects has led to the emergence of multidrug-resistant (MDR) strains of Mycobacterium tuberculosis (M. tb) that are difficult to treat. A current therapeutic stra...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.01677

    authors: Hernandez J,Ashley D,Cao R,Abrahem R,Nguyen T,To K,Yegiazaryan A,Akinwale David A,Kumar Tiwari R,Venketaraman V

    更新日期:2020-08-13 00:00:00

  • Collectin-11 (CL-11) Is a Major Sentinel at Epithelial Surfaces and Key Pattern Recognition Molecule in Complement-Mediated Ischaemic Injury.

    abstract::The complement system is a dynamic subset of the innate immune system, playing roles in host defense, clearance of immune complexes and cell debris, and priming the adaptive immune response. Over the last 40 years our understanding of the complement system has evolved from identifying its presence and recognizing its ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2018.02023

    authors: Nauser CL,Howard MC,Fanelli G,Farrar CA,Sacks S

    更新日期:2018-09-06 00:00:00

  • Establishment and Maintenance of Conventional and Circulation-Driven Lung-Resident Memory CD8+ T Cells Following Respiratory Virus Infections.

    abstract::Antigen-specific CD8+ tissue-resident memory T cells (TRM cells) persist in the lung following resolution of a respiratory virus infection and provide first-line defense against reinfection. In contrast to other memory T cell populations, such as central memory T cells that circulate between lymph and blood, and effec...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2019.00733

    authors: Takamura S,Kohlmeier JE

    更新日期:2019-04-05 00:00:00

  • Single-Cell RNA-Sequencing Shift in the Interaction Pattern Between Glioma Stem Cells and Immune Cells During Tumorigenesis.

    abstract::Glioblastoma is one of the most common neoplasms in the central nervous system characterized by limited immune response and unlimited expansion capability. Cancer stem cells (GSCs), a small fraction of the tumor cells, possess a pivotal regulation capability in the tumor microenvironment with a superior proliferation ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.581209

    authors: Zhai Y,Li G,Li R,Chang Y,Feng Y,Wang D,Wu F,Zhang W

    更新日期:2020-10-08 00:00:00

  • How Mucosal Epithelia Deal with Stress: Role of NKG2D/NKG2D Ligands during Inflammation.

    abstract::Mucosal epithelia encounter both physicochemical and biological stress during their life and have evolved several mechanisms to deal with them, including regulation of immune cell functions. Stressed and damaged cells need to be cleared to control local inflammation and trigger tissue healing. Engagement of the activa...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2017.01583

    authors: Antonangeli F,Soriani A,Cerboni C,Sciumè G,Santoni A

    更新日期:2017-11-20 00:00:00

  • Novel Genome-Editing Tools to Model and Correct Primary Immunodeficiencies.

    abstract::Severe combined immunodeficiency (SCID) and other severe non-SCID primary immunodeficiencies (non-SCID PID) can be treated by allogeneic hematopoietic stem cell (HSC) transplantation, but when histocompatibility leukocyte antigen-matched donors are lacking, this can be a high-risk procedure. Correcting the patient's o...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2015.00250

    authors: Ott de Bruin LM,Volpi S,Musunuru K

    更新日期:2015-05-21 00:00:00

  • Expression and Localization of Paneth Cells and Their α-Defensins in the Small Intestine of Adult Mouse.

    abstract::Paneth cells contribute to intestinal innate immunity by sensing bacteria and secreting α-defensin. In Institute of Cancer Research (ICR) mice, α-defensin termed cryptdin (Crp) in Paneth cells consists of six major isoforms, Crp1 to 6. Despite accumulating evidences that α-defensin functions in controlling the intesti...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.570296

    authors: Nakamura K,Yokoi Y,Fukaya R,Ohira S,Shinozaki R,Nishida T,Kikuchi M,Ayabe T

    更新日期:2020-10-13 00:00:00

  • Longer Duration of SARS-CoV-2 Infection in a Case of Mild COVID-19 With Weak Production of the Specific IgM and IgG Antibodies.

    abstract::Background: The relationship between SARS-CoV-2-carrying time and specific antibody production has not yet been reported in re-admitted COVID-19 patients. We reported a case of mild COVID-19 with long virus-carrying time, weak production of virus-specific IgG and IgM antibodies, and recurrence of positive SARS-CoV-2 R...

    journal_title:Frontiers in immunology

    pub_type:

    doi:10.3389/fimmu.2020.01936

    authors: Guo X,Zeng L,Huang Z,He Y,Zhang Z,Zhong Z

    更新日期:2020-08-07 00:00:00

  • Molecular Landscape of Anti-Drug Antibodies Reveals the Mechanism of the Immune Response Following Treatment With TNFα Antagonists.

    abstract::Drugs formulated from monoclonal antibodies (mAbs) are clinically effective in various diseases. Repeated administration of mAbs, however, elicits an immune response in the form of anti-drug-antibodies (ADA), thereby reducing the drug's efficacy. Notwithstanding their importance, the molecular landscape of ADA and the...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2019.02921

    authors: Vaisman-Mentesh A,Rosenstein S,Yavzori M,Dror Y,Fudim E,Ungar B,Kopylov U,Picard O,Kigel A,Ben-Horin S,Benhar I,Wine Y

    更新日期:2019-12-18 00:00:00

  • Th17 Cells Paradoxical Roles in Melanoma and Potential Application in Immunotherapy.

    abstract::The progressive infiltration of immune cells is associated with the progression of melanoma. Specifically, Th17 cells in melanoma microenvironment have both antitumor and protumor effects. It is now necessary to understand the contradictory data associated with how Th17 cells play a role in melanoma. This review will ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2019.00187

    authors: Chen C,Gao FH

    更新日期:2019-02-08 00:00:00

  • Endoplasmic Reticulum Stress Sensor IRE1α Enhances IL-23 Expression by Human Dendritic Cells.

    abstract::Human monocyte-derived dendritic cells (DCs) exposed to pathogen-associated molecular patterns (PAMPs) undergo bioenergetic changes that influence the immune response. We found that stimulation with PAMPs enhanced glycolysis in DCs, whereas oxidative phosphorylation remained unaltered. Glucose starvation and the hexok...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2017.00639

    authors: Márquez S,Fernández JJ,Terán-Cabanillas E,Herrero C,Alonso S,Azogil A,Montero O,Iwawaki T,Cubillos-Ruiz JR,Fernández N,Crespo MS

    更新日期:2017-06-19 00:00:00

  • Metabolic Flexibility and Innate Immunity in Renal Ischemia Reperfusion Injury: The Fine Balance Between Adaptive Repair and Tissue Degeneration.

    abstract::Renal ischemia reperfusion injury (IRI), a common event after renal transplantation, causes acute kidney injury (AKI), increases the risk of delayed graft function (DGF), primes the donor kidney for rejection, and contributes to the long-term risk of graft loss. In the last decade, epidemiological studies have linked ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2020.01346

    authors: Tammaro A,Kers J,Scantlebery AML,Florquin S

    更新日期:2020-07-07 00:00:00

  • Overexpression of Bovine FcRn in Mice Enhances T-Dependent Immune Responses by Amplifying T Helper Cell Frequency and Germinal Center Enlargement in the Spleen.

    abstract::The neonatal Fc receptor (FcRn) plays key roles in IgG and albumin homeostasis, maternal IgG transport, and antigen presentation of IgG-opsonized antigens. Previously, we reported that transgenic (Tg) mice that overexpress the bovine FcRn (bFcRn) have augmented T-dependent humoral immune response with increased IgG pr...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2015.00357

    authors: Schneider Z,Jani PK,Szikora B,Végh A,Kövesdi D,Iliás A,Cervenak J,Balogh P,Kurucz I,Kacskovics I

    更新日期:2015-07-20 00:00:00

  • Tracing Antibody Repertoire Evolution by Systems Phylogeny.

    abstract::Antibody evolution studies have been traditionally limited to either tracing a single clonal lineage (B cells derived from a single V-(D)-J recombination) over time or examining bulk functionality changes (e.g., tracing serum polyclonal antibody proteins). Studying a single B cell disregards the majority of the humora...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2018.02149

    authors: Yermanos AD,Dounas AK,Stadler T,Oxenius A,Reddy ST

    更新日期:2018-10-02 00:00:00

  • Paving the Way Towards Universal Chimeric Antigen Receptor Therapy in Cancer Treatment: Current Landscape and Progress.

    abstract::Chimeric antigen receptor (CAR) therapy has been proved effective in a stream of clinical trials, especially in hematologic malignancies. However, current CAR therapy is highly personalized as cells used are derived from patients themselves, which can be costly, time-consuming, and sometimes fails to achieve optimal t...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2020.604915

    authors: Zhang Y,Li P,Fang H,Wang G,Zeng X

    更新日期:2020-12-10 00:00:00

  • Dermatomyositis and Immune-Mediated Necrotizing Myopathies: A Window on Autoimmunity and Cancer.

    abstract::Autoimmune myopathies (myositides) are strongly associated with malignancy. The link between myositis and cancer, originally noticed by Bohan and Peter in their classification in 1975 (1), has been evidenced by large population-based cohort studies and a recent meta-analysis. The numerous reports of cases in which the...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2017.00992

    authors: Aussy A,Boyer O,Cordel N

    更新日期:2017-08-21 00:00:00

  • Does NETosis Contribute to the Bacterial Pathoadaptation in Cystic Fibrosis?

    abstract::Significant advances in our understanding of neutrophil biology were made in the past several years. The exciting discovery that neutrophils deploy neutrophil extracellular traps (NETs) to catch pathogens paved the way for a series of additional studies to define the molecular mechanisms of NET generation and the biol...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2014.00378

    authors: Rahman S,Gadjeva M

    更新日期:2014-08-11 00:00:00

  • A Role for Dogs in Advancing Cancer Immunotherapy Research.

    abstract::While rodent cancer models are essential for early proof-of-concept and mechanistic studies for immune therapies, these models have limitations with regards to predicting the ultimate effectiveness of new immunotherapies in humans. As a unique spontaneous, large animal model of cancer, the value of conducting studies ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2019.02935

    authors: Dow S

    更新日期:2020-01-17 00:00:00

  • Recombinant ATPase of Virulent Aeromonas hydrophila Protects Channel Catfish Against Motile Aeromonas Septicemia.

    abstract::Channel catfish farming dominates the aquaculture industry in the United States. However, epidemic outbreaks of motile Aeromonas septicemia (MAS), caused by virulent Aeromonas hydrophila (vAh), have become a prominent problem in the catfish industry. Although vaccination is an effective preventive method, there is no ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2019.01641

    authors: Abdelhamed H,Banes M,Karsi A,Lawrence ML

    更新日期:2019-07-16 00:00:00

  • Mediation of Mucosal Immunoglobulins in Buccal Cavity of Teleost in Antibacterial Immunity.

    abstract::The buccal mucosa (BM) of vertebrates is a critical mucosal barrier constantly exposed to rich and diverse pathogens from air, water, and food. While mammals are known to contain a mucosal associated lymphoid tissue (MALT) in the buccal cavity which induces B-cells and immunoglobulins (Igs) responses against bacterial...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.562795

    authors: Xu HY,Dong F,Zhai X,Meng KF,Han GK,Cheng GF,Wu ZB,Li N,Xu Z

    更新日期:2020-09-23 00:00:00

  • Post-Aire maturation of thymic medullary epithelial cells involves selective expression of keratinocyte-specific autoantigens.

    abstract::The autoimmune regulator (Aire)-directed ectopic expression of tissue-specific antigens (TSAs) by mature medullary thymic epithelial cells (mTECs) has been viewed as an essential mechanism in the induction of central tolerance. Recent data suggest that the survival of mTECs extends beyond the Aire+ cell population to ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2012.00019

    authors: Wang X,Laan M,Bichele R,Kisand K,Scott HS,Peterson P

    更新日期:2012-03-01 00:00:00

  • Meta-Analysis of in vitro-Differentiated Macrophages Identifies Transcriptomic Signatures That Classify Disease Macrophages in vivo.

    abstract::Macrophages are heterogeneous leukocytes regulated in a tissue- and disease-specific context. While in vitro macrophage models have been used to study diseases empirically, a systematic analysis of the transcriptome thereof is lacking. Here, we acquired gene expression data from eight commonly-used in vitro macrophage...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,meta分析

    doi:10.3389/fimmu.2019.02887

    authors: Chen HJ,Li Yim AYF,Griffith GR,de Jonge WJ,Mannens MMAM,Ferrero E,Henneman P,de Winther MPJ

    更新日期:2019-12-11 00:00:00

  • Microthrombotic Renal Vascular Lesions Are Associated to Increased Renal Inflammatory Infiltration in Murine Lupus Nephritis.

    abstract::Background: Vascular microthrombotic lesions in lupus nephritis with or without antiphospholipid antibodies may relate to worse renal outcomes. Whether microthrombotic lesions are a consequence of renal inflammation or independently contribute to renal damage is unclear. Our aim was to investigate the relationship bet...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2018.01948

    authors: Gonzalo-Gil E,García-Herrero C,Toldos O,Usategui A,Criado G,Pérez-Yagüe S,Barber DF,Pablos JL,Galindo M

    更新日期:2018-08-28 00:00:00

  • Intestinal Microbiota Influence Immune Tolerance Post Allogeneic Hematopoietic Cell Transplantation and Intestinal GVHD.

    abstract::Under normal conditions our intestines are inhabited by trillions of diverse microorganisms composing the intestinal microbiota, which are mostly non-pathogenic anaerobic commensal bacteria vital for the maintenance of immune homeostasis. The composition and diversity of the intestinal microbiota can be disturbed by v...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2018.03179

    authors: Köhler N,Zeiser R

    更新日期:2019-01-17 00:00:00

  • iCa2+ Flux, ROS and IL-10 Determines Cytotoxic, and Suppressor T Cell Functions in Chronic Human Viral Infections.

    abstract::Exhaustion of CD8+ T cells and increased IL-10 production is well-known in chronic viral infections but mechanisms leading to loss of their cytotoxic capabilities and consequent exhaustion remain unclear. Exhausted CD8+T cells also called T suppressors are highly immune suppressive with altered T cell receptor signali...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章

    doi:10.3389/fimmu.2020.00083

    authors: Mohanty S,Barik P,Debata N,Nagarajan P,Devadas S

    更新日期:2020-03-06 00:00:00

  • Cutaneous Leishmaniasis Vaccination: A Matter of Quality.

    abstract::There have been exhaustive efforts to develop an efficient vaccine against leishmaniasis. Factors like host and parasite genetic characteristics, virulence, epidemiological scenarios, and, mainly, diverse immune responses triggered by Leishmania species make the achievement of this aim a complex task. It is already cl...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审

    doi:10.3389/fimmu.2016.00151

    authors: De Luca PM,Macedo AB

    更新日期:2016-04-21 00:00:00