Immunogenicity and Serological Cross-Reactivity of Saliva Proteins among Different Tsetse Species.


:Tsetse are vectors of pathogenic trypanosomes, agents of human and animal trypanosomiasis in Africa. Components of tsetse saliva (sialome) are introduced into the mammalian host bite site during the blood feeding process and are important for tsetse's ability to feed efficiently, but can also influence disease transmission and serve as biomarkers for host exposure. We compared the sialome components from four tsetse species in two subgenera: subgenus Morsitans: Glossina morsitans morsitans (Gmm) and Glossina pallidipes (Gpd), and subgenus Palpalis: Glossina palpalis gambiensis (Gpg) and Glossina fuscipes fuscipes (Gff), and evaluated their immunogenicity and serological cross reactivity by an immunoblot approach utilizing antibodies from experimental mice challenged with uninfected flies. The protein and immune profiles of sialome components varied with fly species in the same subgenus displaying greater similarity and cross reactivity. Sera obtained from cattle from disease endemic areas of Africa displayed an immunogenicity profile reflective of tsetse species distribution. We analyzed the sialome fractions of Gmm by LC-MS/MS, and identified TAg5, Tsal1/Tsal2, and Sgp3 as major immunogenic proteins, and the 5'-nucleotidase family as well as four members of the Adenosine Deaminase Growth Factor (ADGF) family as the major non-immunogenic proteins. Within the ADGF family, we identified four closely related proteins (TSGF-1, TSGF-2, ADGF-3 and ADGF-4), all of which are expressed in tsetse salivary glands. We describe the tsetse species-specific expression profiles and genomic localization of these proteins. Using a passive-immunity approach, we evaluated the effects of rec-TSGF (TSGF-1 and TSGF-2) polyclonal antibodies on tsetse fitness parameters. Limited exposure of tsetse to mice with circulating anti-TSGF antibodies resulted in a slight detriment to their blood feeding ability as reflected by compromised digestion, lower weight gain and less total lipid reserves although these results were not statistically significant. Long-term exposure studies of tsetse flies to antibodies corresponding to the ADGF family of proteins are warranted to evaluate the role of this conserved family in fly biology.


PLoS Negl Trop Dis


Zhao X,Alves e Silva TL,Cronin L,Savage AF,O'Neill M,Nerima B,Okedi LM,Aksoy S




Has Abstract


2015-08-27 00:00:00














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    pub_type: 杂志文章


    authors: Singh R,Singh B,Mahato S

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    pub_type: 杂志文章


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    authors: Hoppenheit A,Murugaiyan J,Bauer B,Steuber S,Clausen PH,Roesler U

    更新日期:2013-07-11 00:00:00

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    pub_type: 杂志文章,评审


    authors: Kingsley PV,Leader M,Nagodawithana NS,Tipre M,Sathiakumar N

    更新日期:2016-12-22 00:00:00

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    authors: Won KY,Robinson K,Hamlin KL,Tufa J,Seespesara M,Wiegand RE,Gass K,Kubofcik J,Nutman TB,Lammie PJ,Fuimaono S

    更新日期:2018-03-09 00:00:00

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    authors: Openshaw JJ,Medina A,Felt SA,Li T,Huan Z,Rozelle S,Luby SP

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    authors: Bobadilla-del Valle M,Torres-González P,Cervera-Hernández ME,Martínez-Gamboa A,Crabtree-Ramirez B,Chávez-Mazari B,Ortiz-Conchi N,Rodríguez-Cruz L,Cervantes-Sánchez A,Gudiño-Enríquez T,Cinta-Severo C,Sifuentes-Osornio J,Ponce de L

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    authors: Bello SA,Adeoye JA,Oketade I,Akadiri OA

    更新日期:2019-07-22 00:00:00

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    pub_type: 杂志文章,评审


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    更新日期:2016-09-15 00:00:00

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    journal_title:PLoS neglected tropical diseases

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    abstract:BACKGROUND:Schistosomiasis in one of the most prevalent parasitic diseases, affecting millions of people and animals in developing countries. Amongst the human-infective species S. haematobium is one of the most widespread causing urogenital schistosomiasis, a major human health problem across Africa, however in terms ...

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    pub_type: 杂志文章


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    abstract:BACKGROUND:Dengue virus (DENV) is the most important arbovirus worldwide, causing infections in endemic countries and returning travellers from these areas. Rapid diagnostic tests are needed to improve patient management and monitor local transmission. The detection of DENV non-structural protein 1 (NS1) is a useful to...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Alejo-Cancho I,Navero-Castillejos J,Peiró-Mestres A,Albarracín R,Barrachina J,Navarro A,Gonzalo V,Pastor V,Muñoz J,Martínez MJ

    更新日期:2020-02-18 00:00:00

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    authors: Huang XH,Qian MB,Zhu GH,Fang YY,Hao YT,Lai YS

    更新日期:2020-03-27 00:00:00

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Ferede G,Tiruneh M,Abate E,Wondimeneh Y,Damtie D,Gadisa E,Howe R,Aseffa A,Tessema B

    更新日期:2018-05-31 00:00:00

  • Gene flow, subspecies composition, and dengue virus-2 susceptibility among Aedes aegypti collections in Senegal.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Sylla M,Bosio C,Urdaneta-Marquez L,Ndiaye M,Black WC 4th

    更新日期:2009-01-01 00:00:00

  • The evolutionary history and spatiotemporal dynamics of the fever, thrombocytopenia and leukocytopenia syndrome virus (FTLSV) in China.

    abstract:BACKGROUND:In 2007, a novel bunyavirus was found in Henan Province, China and named fever, thrombocytopenia and leukocytopenia syndrome virus (FTLSV); since then, FTLSV has been found in ticks and animals in many Chinese provinces. Human-to-human transmission has been documented, indicating that FTLSV should be conside...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Huang X,Liu L,Du Y,Wu W,Wang H,Su J,Tang X,Liu Q,Yang Y,Jiang Y,Chen W,Xu B

    更新日期:2014-10-16 00:00:00

  • Mp1p Is a Virulence Factor in Talaromyces (Penicillium) marneffei.

    abstract:BACKGROUND:Talaromyces marneffei is an opportunistic dimorphic fungus prevalent in Southeast Asia. We previously demonstrated that Mp1p is an immunogenic surface and secretory mannoprotein of T. marneffei. Since Mp1p is a surface protein that can generate protective immunity, we hypothesized that Mp1p and/or its homolo...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Woo PC,Lau SK,Lau CC,Tung ET,Chong KT,Yang F,Zhang H,Lo RK,Cai JP,Au-Yeung RK,Ng WF,Tse H,Wong SS,Xu S,Lam WH,Tse MK,Sze KH,Kao RY,Reiner NE,Hao Q,Yuen KY

    更新日期:2016-08-25 00:00:00

  • A new model for management of mycetoma in the Sudan.

    abstract::Patients with mycetoma usually present late with advanced disease, which is attributed to lack of medical and health facilities in endemic areas, poor health education and low socio-economic status. With this background, an integrated patient management model at the village level was designed to address the various pr...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fahal A,Mahgoub el S,El Hassan AM,Abdel-Rahman ME,Alshambaty Y,Hashim A,Hago A,Zijlstra EE

    更新日期:2014-10-30 00:00:00

  • The iron distribution and magnetic properties of schistosome eggshells: implications for improved diagnostics.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Karl S,Gutiérrez L,Lucyk-Maurer R,Kerr R,Candido RR,Toh SQ,Saunders M,Shaw JA,Suvorova A,Hofmann A,House MJ,Woodward RC,Graeff-Teixera C,St Pierre TG,Jones MK

    更新日期:2013-05-16 00:00:00

  • Cost-effectiveness of chagas disease vector control strategies in Northwestern Argentina.

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    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Vazquez-Prokopec GM,Spillmann C,Zaidenberg M,Kitron U,Gürtler RE

    更新日期:2009-01-01 00:00:00

  • Evaluation of a novel West Nile virus transmission control strategy that targets Culex tarsalis with endectocide-containing blood meals.

    abstract::Control of arbovirus transmission remains focused on vector control through application of insecticides directly to the environment. However, these insecticide applications are often reactive interventions that can be poorly-targeted, inadequate for localized control during outbreaks, and opposed due to environmental ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Nguyen C,Gray M,Burton TA,Foy SL,Foster JR,Gendernalik AL,Rückert C,Alout H,Young MC,Boze B,Ebel GD,Clapsaddle B,Foy BD

    更新日期:2019-03-07 00:00:00