Abstract:
:Tsetse are vectors of pathogenic trypanosomes, agents of human and animal trypanosomiasis in Africa. Components of tsetse saliva (sialome) are introduced into the mammalian host bite site during the blood feeding process and are important for tsetse's ability to feed efficiently, but can also influence disease transmission and serve as biomarkers for host exposure. We compared the sialome components from four tsetse species in two subgenera: subgenus Morsitans: Glossina morsitans morsitans (Gmm) and Glossina pallidipes (Gpd), and subgenus Palpalis: Glossina palpalis gambiensis (Gpg) and Glossina fuscipes fuscipes (Gff), and evaluated their immunogenicity and serological cross reactivity by an immunoblot approach utilizing antibodies from experimental mice challenged with uninfected flies. The protein and immune profiles of sialome components varied with fly species in the same subgenus displaying greater similarity and cross reactivity. Sera obtained from cattle from disease endemic areas of Africa displayed an immunogenicity profile reflective of tsetse species distribution. We analyzed the sialome fractions of Gmm by LC-MS/MS, and identified TAg5, Tsal1/Tsal2, and Sgp3 as major immunogenic proteins, and the 5'-nucleotidase family as well as four members of the Adenosine Deaminase Growth Factor (ADGF) family as the major non-immunogenic proteins. Within the ADGF family, we identified four closely related proteins (TSGF-1, TSGF-2, ADGF-3 and ADGF-4), all of which are expressed in tsetse salivary glands. We describe the tsetse species-specific expression profiles and genomic localization of these proteins. Using a passive-immunity approach, we evaluated the effects of rec-TSGF (TSGF-1 and TSGF-2) polyclonal antibodies on tsetse fitness parameters. Limited exposure of tsetse to mice with circulating anti-TSGF antibodies resulted in a slight detriment to their blood feeding ability as reflected by compromised digestion, lower weight gain and less total lipid reserves although these results were not statistically significant. Long-term exposure studies of tsetse flies to antibodies corresponding to the ADGF family of proteins are warranted to evaluate the role of this conserved family in fly biology.
journal_name
PLoS Negl Trop Disjournal_title
PLoS neglected tropical diseasesauthors
Zhao X,Alves e Silva TL,Cronin L,Savage AF,O'Neill M,Nerima B,Okedi LM,Aksoy Sdoi
10.1371/journal.pntd.0004038subject
Has Abstractpub_date
2015-08-27 00:00:00pages
e0004038issue
8eissn
1935-2727issn
1935-2735pii
PNTD-D-15-00752journal_volume
9pub_type
杂志文章abstract:BACKGROUND:Leptospirosis occurs worldwide, but the global incidence of human disease and its mortality are not well understood. Many patients are undiagnosed and untreated due to its non-specific symptoms and a lack of access to diagnostics. This study systematically reviews the literature to clarify the mortality from...
journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,评审
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章,随机对照试验
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章,随机对照试验
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2014-04-24 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2016-12-30 00:00:00
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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abstract::[This corrects the article DOI: 10.1371/journal.pntd.0005006.]. ...
journal_title:PLoS neglected tropical diseases
pub_type: 已发布勘误
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更新日期:2016-12-20 00:00:00