Directed evaluation of enterotoxigenic Escherichia coli autotransporter proteins as putative vaccine candidates.

Abstract:

BACKGROUND:Enterotoxigenic Escherichia coli (ETEC) is a major diarrheal pathogen in developing countries, where it accounts for millions of infections and hundreds of thousands of deaths annually. While vaccine development to prevent diarrheal illness due to ETEC is feasible, extensive effort is needed to identify conserved antigenic targets. Pathogenic Escherichia coli, including ETEC, use the autotransporter (AT) secretion mechanism to export virulence factors. AT proteins are comprised of a highly conserved carboxy terminal outer membrane beta barrel and a surface-exposed amino terminal passenger domain. Recent immunoproteomic studies suggesting that multiple autotransporter passenger domains are recognized during ETEC infection prompted the present studies. METHODOLOGY:Available ETEC genomes were examined to identify AT coding sequences present in pathogenic isolates, but not in the commensal E. coli HS strain. Passenger domains of the corresponding autotransporters were cloned and expressed as recombinant antigens, and the immune response to these proteins was then examined using convalescent sera from patients and experimentally infected mice. PRINCIPAL FINDINGS:Potential AT genes shared by ETEC strains, but absent in the E. coli commensal HS strain were identified. Recombinant passenger domains derived from autotransporters, including Ag43 and an AT designated pAT, were recognized by antibodies from mice following intestinal challenge with H10407, and both Ag43 and pAT were identified on the surface of ETEC by flow cytometry. Likewise, convalescent sera from patients with ETEC diarrhea recognized Ag43 and pAT, suggesting that these proteins are expressed during both experimental and naturally occurring ETEC infections and that they are immunogenic. Vaccination of mice with recombinant passenger domains from either pAT or Ag43 afforded protection against intestinal colonization with ETEC. CONCLUSIONS:Passenger domains of conserved autotransporter proteins could contribute to protective immune responses that develop following infection with ETEC, and these antigens consequently represent potential targets to explore in vaccine development.

journal_name

PLoS Negl Trop Dis

authors

Harris JA,Roy K,Woo-Rasberry V,Hamilton DJ,Kansal R,Qadri F,Fleckenstein JM

doi

10.1371/journal.pntd.0001428

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

e1428

issue

12

eissn

1935-2727

issn

1935-2735

pii

PNTD-D-11-00386

journal_volume

5

pub_type

杂志文章
  • Testing the efficacy of a multi-component DNA-prime/DNA-boost vaccine against Trypanosoma cruzi infection in dogs.

    abstract:BACKGROUND:Trypanosoma cruzi, the etiologic agent of Chagas Disease, is a major vector borne health problem in Latin America and an emerging infectious disease in the United States. METHODS:We tested the efficacy of a multi-component DNA-prime/DNA-boost vaccine (TcVac1) against experimental T. cruzi infection in a can...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0001050

    authors: Aparicio-Burgos JE,Ochoa-García L,Zepeda-Escobar JA,Gupta S,Dhiman M,Martínez JS,de Oca-Jiménez RM,Val Arreola M,Barbabosa-Pliego A,Vázquez-Chagoyán JC,Garg NJ

    更新日期:2011-01-01 00:00:00

  • Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil.

    abstract:BACKGROUND:Chromoblastomycosis (CBM) is a difficult-to-treat chronic subcutaneous mycosis. In Brazil, the main agent of this disease is Fonsecaea pedrosoi, which is phenotypically very similar to other Fonsecaea species, differing only genetically. The correct species identification is relevant since different species ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006675

    authors: Coelho RA,Brito-Santos F,Figueiredo-Carvalho MHG,Silva JVDS,Gutierrez-Galhardo MC,do Valle ACF,Zancopé-Oliveira RM,Trilles L,Meyer W,Freitas DFS,Almeida-Paes R

    更新日期:2018-07-26 00:00:00

  • Immunogenicity and Serological Cross-Reactivity of Saliva Proteins among Different Tsetse Species.

    abstract::Tsetse are vectors of pathogenic trypanosomes, agents of human and animal trypanosomiasis in Africa. Components of tsetse saliva (sialome) are introduced into the mammalian host bite site during the blood feeding process and are important for tsetse's ability to feed efficiently, but can also influence disease transmi...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0004038

    authors: Zhao X,Alves e Silva TL,Cronin L,Savage AF,O'Neill M,Nerima B,Okedi LM,Aksoy S

    更新日期:2015-08-27 00:00:00

  • A serologic study of dengue in northwest Ethiopia: Suggesting preventive and control measures.

    abstract:BACKGROUND:Dengue is one of the most serious and rapidly spreading arboviral diseases in the world. Despite many acute febrile illnesses in Ethiopia, the burden of illness due to dengue in the country is largely unknown. Thus, the present study aimed to provide the first baseline data on seroprevalence and associated r...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006430

    authors: Ferede G,Tiruneh M,Abate E,Wondimeneh Y,Damtie D,Gadisa E,Howe R,Aseffa A,Tessema B

    更新日期:2018-05-31 00:00:00

  • Distribution and seasonal fluctuations of Ae. aegypti and Ae. albopictus larval and pupae in residential areas in an urban landscape.

    abstract::Dengue, a vector-borne disease spread by Aedes mosquitoes, is a global threat. In the absence of an efficacious dengue vaccine, vector control is the key intervention tool in Singapore. A good understanding of vector habitats is essential to formulate operational strategies. We examined the distribution, long-term tre...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008209

    authors: Rajarethinam J,Ong J,Neo ZW,Ng LC,Aik J

    更新日期:2020-04-20 00:00:00

  • Evaluation of a novel microfluidic immuno-magnetic agglutination assay method for detection of dengue virus NS1 antigen.

    abstract:BACKGROUND:Dengue virus (DENV) is the most important arbovirus worldwide, causing infections in endemic countries and returning travellers from these areas. Rapid diagnostic tests are needed to improve patient management and monitor local transmission. The detection of DENV non-structural protein 1 (NS1) is a useful to...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008082

    authors: Alejo-Cancho I,Navero-Castillejos J,Peiró-Mestres A,Albarracín R,Barrachina J,Navarro A,Gonzalo V,Pastor V,Muñoz J,Martínez MJ

    更新日期:2020-02-18 00:00:00

  • Immune evasion and recognition of the syphilis spirochete in blood and skin of secondary syphilis patients: two immunologically distinct compartments.

    abstract:BACKGROUND:The clinical syndrome associated with secondary syphilis (SS) reflects the propensity of Treponema pallidum (Tp) to escape immune recognition while simultaneously inducing inflammation. METHODS:To better understand the duality of immune evasion and immune recognition in human syphilis, herein we used a comb...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0001717

    authors: Cruz AR,Ramirez LG,Zuluaga AV,Pillay A,Abreu C,Valencia CA,La Vake C,Cervantes JL,Dunham-Ems S,Cartun R,Mavilio D,Radolf JD,Salazar JC

    更新日期:2012-01-01 00:00:00

  • Host immune responses induced by specific Mycobacterium leprae antigens in an overnight whole-blood assay correlate with the diagnosis of paucibacillary leprosy patients in China.

    abstract:BACKGROUND:Leprosy, caused by Mycobacterium leprae, affects over 200,000 people annually worldwide and remains endemic in the ethnically diverse, mountainous and underdeveloped southwestern provinces of China. Delayed diagnosis of leprosy persists in China, thus, additional knowledge to support early diagnosis, especia...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007318

    authors: Chen X,You YG,Yuan YH,Yuan LC,Wen Y

    更新日期:2019-04-24 00:00:00

  • Plasmodium vivax but Not Plasmodium falciparum Blood-Stage Infection in Humans Is Associated with the Expansion of a CD8+ T Cell Population with Cytotoxic Potential.

    abstract::P. vivax and P. falciparum parasites display different tropism for host cells and induce very different clinical symptoms and pathology, suggesting that the immune responses required for protection may differ between these two species. However, no study has qualitatively compared the immune responses to P. falciparum ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 临床试验,杂志文章

    doi:10.1371/journal.pntd.0005031

    authors: Burel JG,Apte SH,McCarthy JS,Doolan DL

    更新日期:2016-12-08 00:00:00

  • RNA-Seq reveals infection-induced gene expression changes in the snail intermediate host of the carcinogenic liver fluke, Opisthorchis viverrini.

    abstract:BACKGROUND:Bithynia siamensis goniomphalos is the snail intermediate host of the liver fluke, Opisthorchis viverrini, the leading cause of cholangiocarcinoma (CCA) in the Greater Mekong sub-region of Thailand. Despite the severe public health impact of Opisthorchis-induced CCA, knowledge of the molecular interactions o...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002765

    authors: Prasopdee S,Sotillo J,Tesana S,Laha T,Kulsantiwong J,Nolan MJ,Loukas A,Cantacessi C

    更新日期:2014-03-27 00:00:00

  • Female-specific flightless (fsRIDL) phenotype for control of Aedes albopictus.

    abstract:BACKGROUND:Aedes albopictus, the Asian tiger mosquito, is a vector of several arboviruses including dengue and chikungunya, and is also a significant nuisance mosquito. It is one of the most invasive of mosquitoes with a relentlessly increasing geographic distribution. Conventional control methods have so far failed to...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0001724

    authors: Labbé GM,Scaife S,Morgan SA,Curtis ZH,Alphey L

    更新日期:2012-01-01 00:00:00

  • Optimal timing of insecticide fogging to minimize dengue cases: modeling dengue transmission among various seasonalities and transmission intensities.

    abstract:BACKGROUND:Dengue infection is endemic in many regions throughout the world. While insecticide fogging targeting the vector mosquito Aedes aegypti is a major control measure against dengue epidemics, the impact of this method remains controversial. A previous mathematical simulation study indicated that insecticide fog...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0001367

    authors: Oki M,Sunahara T,Hashizume M,Yamamoto T

    更新日期:2011-10-01 00:00:00

  • Viral etiology of encephalitis in children in southern Vietnam: results of a one-year prospective descriptive study.

    abstract:BACKGROUND:Acute encephalitis is an important and severe disease in children in Vietnam. However, little is known about the etiology while such knowledge is essential for optimal prevention and treatment. To identify viral causes of encephalitis, in 2004 we conducted a one-year descriptive study at Children's Hospital ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0000854

    authors: Le VT,Phan TQ,Do QH,Nguyen BH,Lam QB,Bach V,Truong H,Tran TH,Nguyen V,Tran T,Vo M,Tran VT,Schultsz C,Farrar J,van Doorn HR,de Jong MD

    更新日期:2010-10-26 00:00:00

  • Sero-Molecular Epidemiology of Japanese Encephalitis in Zhejiang, an Eastern Province of China.

    abstract:BACKGROUND:Sporadic Japanese encephalitis (JE) cases still have been reported in Zhejiang Province in recent years, and concerns about vaccine cross-protection and population-level immunity have been raised off and on within the public health sphere. Genotype I (GI) has replaced GIII as the dominant genotype in Asian c...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0004936

    authors: Pan JR,Yan JY,Zhou JY,Tang XW,He HQ,Xie RH,Mao HY,Zhang YJ,Xie SY

    更新日期:2016-08-25 00:00:00

  • The human-snail transmission environment shapes long term schistosomiasis control outcomes: Implications for improving the accuracy of predictive modeling.

    abstract:INTRODUCTION:Schistosomiasis is a chronic parasitic trematode disease that affects over 240 million people worldwide. The Schistosoma lifecycle is complex, involving transmission via specific intermediate-host freshwater snails. Predictive mathematical models of Schistosoma transmission have often chosen to simplify or...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0006514

    authors: Gurarie D,Lo NC,Ndeffo-Mbah ML,Durham DP,King CH

    更新日期:2018-05-21 00:00:00

  • Nelfinavir, an HIV-1 protease inhibitor, induces oxidative stress-mediated, caspase-independent apoptosis in Leishmania amastigotes.

    abstract:BACKGROUND:Visceral leishmaniasis has now emerged as an important opportunistic disease in patients coinfected with human immunodeficiency virus type-1 (HIV-1). Although the effectiveness of HIV-1 protease inhibitors, such as nelfinavir, in antiretroviral therapies is well documented, little is known of the impact of t...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0000642

    authors: Kumar P,Lodge R,Trudel N,Ouellet M,Ouellette M,Tremblay MJ

    更新日期:2010-03-30 00:00:00

  • Chagas disease, migration and community settlement patterns in Arequipa, Peru.

    abstract:BACKGROUND:Chagas disease is one of the most important neglected tropical diseases in the Americas. Vectorborne transmission of Chagas disease has been historically rare in urban settings. However, in marginal communities near the city of Arequipa, Peru, urban transmission cycles have become established. We examined th...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0000567

    authors: Bayer AM,Hunter GC,Gilman RH,Cornejo Del Carpio JG,Naquira C,Bern C,Levy MZ

    更新日期:2009-12-15 00:00:00

  • Evaluation of the loop mediated isothermal DNA amplification (LAMP) kit for malaria diagnosis in P. vivax endemic settings of Colombia.

    abstract:BACKGROUND:Most commonly used malaria diagnostic tests, including microscopy and antigen-detecting rapid tests, cannot reliably detect low-density infections which are frequent in low transmission settings. Molecular methods such as polymerase chain reaction (PCR) are highly sensitive but remain too laborious for field...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0003453

    authors: Vallejo AF,Martínez NL,González IJ,Arévalo-Herrera M,Herrera S

    更新日期:2015-01-08 00:00:00

  • The global burden of alveolar echinococcosis.

    abstract:BACKGROUND:Human alveolar echinococcosis (AE) is known to be common in certain rural communities in China whilst it is generally rare and sporadic elsewhere. The objective of this study was to provide a first estimate of the global incidence of this disease by country. The second objective was to estimate the global di...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审

    doi:10.1371/journal.pntd.0000722

    authors: Torgerson PR,Keller K,Magnotta M,Ragland N

    更新日期:2010-06-22 00:00:00

  • Invariant NKT cell response to dengue virus infection in human.

    abstract:BACKGROUND:Dengue viral infection is a global health threat without vaccine or specific treatment. The clinical outcome varies from asymptomatic, mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF). While adaptive immune responses were found to be detrimental in the dengue pathogenesis, the roles of earlier...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002955

    authors: Matangkasombut P,Chan-In W,Opasawaschai A,Pongchaikul P,Tangthawornchaikul N,Vasanawathana S,Limpitikul W,Malasit P,Duangchinda T,Screaton G,Mongkolsapaya J

    更新日期:2014-06-19 00:00:00

  • Levofloxacin cures experimental pneumonic plague in African green monkeys.

    abstract:BACKGROUND:Yersinia pestis, the agent of plague, is considered a potential bioweapon due to rapid lethality when delivered as an aerosol. Levofloxacin was tested for primary pneumonic plague treatment in a nonhuman primate model mimicking human disease. METHODS AND RESULTS:Twenty-four African Green monkeys (AGMs, Chlo...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0000959

    authors: Layton RC,Mega W,McDonald JD,Brasel TL,Barr EB,Gigliotti AP,Koster F

    更新日期:2011-02-08 00:00:00

  • Yaws resurgence in Bankim, Cameroon: The relative effectiveness of different means of detection in rural communities.

    abstract:BACKGROUND:Yaws is an infectious, debilitating and disfiguring disease of poverty that mainly affects children in rural communities in tropical areas. In Cameroon, mass-treatment campaigns carried out in the 1950s reduced yaws to such low levels that it was presumed the disease was eradicated. In 2010, an epidemiologic...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0005557

    authors: Boock AU,Awah PK,Mou F,Nichter M

    更新日期:2017-05-08 00:00:00

  • Phylogeography of recently emerged DENV-2 in southern Viet Nam.

    abstract::Revealing the dispersal of dengue viruses (DENV) in time and space is central to understanding their epidemiology. However, the processes that shape DENV transmission patterns at the scale of local populations are not well understood, particularly the impact of such factors as human population movement and urbanizatio...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0000766

    authors: Rabaa MA,Ty Hang VT,Wills B,Farrar J,Simmons CP,Holmes EC

    更新日期:2010-07-27 00:00:00

  • New Insights into the Geographic Distribution of Mycobacterium leprae SNP Genotypes Determined for Isolates from Leprosy Cases Diagnosed in Metropolitan France and French Territories.

    abstract:BACKGROUND:Between 20 and 30 bacteriologically confirmed cases of leprosy are diagnosed each year at the French National Reference Center for mycobacteria. Patients are mainly immigrants from various endemic countries or living in French overseas territories. We aimed at expanding data regarding the geographical distri...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0004141

    authors: Reibel F,Chauffour A,Brossier F,Jarlier V,Cambau E,Aubry A

    更新日期:2015-10-06 00:00:00

  • Leptospira Interrogans induces fibrosis in the mouse kidney through Inos-dependent, TLR- and NLR-independent signaling pathways.

    abstract:BACKGROUND:Leptospira (L.) interrogans are bacteria responsible for a worldwide reemerging zoonosis. Rodents carry L. interrogans asymptomatically in their kidneys and excrete bacteria in the urine, contaminating the environment. Humans get infected through skin contact and develop a mild or severe leptospirosis that m...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0002664

    authors: Fanton d'Andon M,Quellard N,Fernandez B,Ratet G,Lacroix-Lamandé S,Vandewalle A,Boneca IG,Goujon JM,Werts C

    更新日期:2014-01-30 00:00:00

  • Antagonistic effects of Plasmodium-helminth co-infections on malaria pathology in different population groups in Côte d'Ivoire.

    abstract:INTRODUCTION:Plasmodium spp. and helminths are co-endemic in many parts of the tropics; hence, co-infection is a common phenomenon. Interactions between Plasmodium and helminth infections may alter the host's immune response and susceptibility and thus impact on morbidity. There is little information on the direction a...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007086

    authors: Hürlimann E,Houngbedji CA,Yapi RB,N'Dri PB,Silué KD,Ouattara M,Utzinger J,N'Goran EK,Raso G

    更新日期:2019-01-10 00:00:00

  • Barriers to Buruli ulcer treatment completion in the Ashanti and Central Regions, Ghana.

    abstract:BACKGROUND:Buruli ulcer is a chronic ulcerating skin condition, with the highest burden found in Central and West Africa where it disproportionately affects the most vulnerable populations. Treatment is demanding, comprising eight-weeks of daily antibiotics, regular wound care and possible surgical intervention. Treatm...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0008369

    authors: Collinson S,Frimpong VNB,Agbavor B,Montgomery B,Oppong M,Frimpong M,Amoako YA,Marks M,Phillips RO

    更新日期:2020-05-26 00:00:00

  • Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva.

    abstract:BACKGROUND:Hosts repeatedly bitten by sand flies develop antibodies against sand fly saliva and screening of these immunoglobulins can be employed to estimate the risk of Leishmania transmission, to indicate the feeding preferences of sand flies, or to evaluate the effectiveness of vector control campaigns. Previously,...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0007078

    authors: Sima M,Ferencova B,Bhattacharyya T,Miles MA,Litvinov SV,Hailu A,Baneth G,Volf P

    更新日期:2019-01-24 00:00:00

  • Head and neck mycetoma: the mycetoma research centre experience.

    abstract::Mycetoma is a unique neglected tropical disease which is endemic in what is known as the "mycetoma belt". The disease has many devastating impacts on patients and communities in endemic area and is characterised by massive deformity, destruction and disability. Mycetoma is commonly seen in the foot and hand and less f...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0003587

    authors: Fahal A,Mahgoub el S,El Hassan AM,Jacoub AO,Hassan D

    更新日期:2015-03-13 00:00:00

  • Identification of microRNA genes in three opisthorchiids.

    abstract:BACKGROUND:Opisthorchis felineus, O. viverrini, and Clonorchis sinensis (family Opisthorchiidae) are parasitic flatworms that pose a serious threat to humans in some countries and cause opisthorchiasis/clonorchiasis. Chronic disease may lead to a risk of carcinogenesis in the biliary ducts. MicroRNAs (miRNAs) are small...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章

    doi:10.1371/journal.pntd.0003680

    authors: Ovchinnikov VY,Afonnikov DA,Vasiliev GV,Kashina EV,Sripa B,Mordvinov VA,Katokhin AV

    更新日期:2015-04-21 00:00:00