Abstract:
:To generate a more efficient in vivo reporter and therapeutic gene, we optimized the coding sequence of the human sodium/iodide symporter (NIS) gene by replacing NIS DNA codons from wild type to new codons having the highest usage in human gene translation. The Codon Adaptation Index (CAI), representing the number of codons effective for human expression, was much improved (0.79 for hNIS, 0.97 for opt-hNIS). Both wild-type (hNIS) and optimized human NIS (opt-hNIS) were cloned into pcDNA3.1 and pMSCV vectors for transfection. Various cancer cell lines such as thyroid (TPC-1, FRO, B-CPAP), breast (MDA-MB-231), liver (Hep3B), cervical (HeLa), and glioma (U87MG) were transfected with pcDNA3.1/hNIS or pcDNA3.1/opt-hNIS. 125I uptake by opt-hNIS-expressing cells was 1.6~2.1 times higher than uptake by wild-type hNIS-expressing cells. Stable cell lines were also established by retroviral transduction using pMSCV/hNIS or pMSCV/opt-hNIS, revealing higher NIS protein levels and 125I uptake in opt-hNIS-expressing cells than in hNIS-expressing cells. Moreover, scintigraphic images from cell plates and mouse xenografts showed stronger signals from opt-hNIS-expressing cells than hNIS-expressing cells, and radioactivity uptake by opt-hNIS-expressing tumors was 2.3-fold greater than that by hNIS-expressing tumors. To test the efficacy of radioiodine therapy, mouse xenograft models were established with cancer cells expressing hNIS or opt-hNIS. 131I treatment reduced tumor sizes of hNIS- and opt-hNIS-expressing tumors to 0.57- and 0.27- fold, respectively, compared to their sizes before therapy, suggesting an improved therapeutic effect of opt-hNIS. In summary, this study shows that codon optimization strongly increases hNIS protein levels and radioiodine uptake, thus supporting opt-hNIS as a more sensitive reporter and efficient therapeutic gene.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Kim YH,Youn H,Na J,Hong KJ,Kang KW,Lee DS,Chung JKdoi
10.7150/thno.10062subject
Has Abstractpub_date
2015-01-01 00:00:00pages
86-96issue
1issn
1838-7640pii
thnov05p0086journal_volume
5pub_type
杂志文章相关文献
Theranostics文献大全abstract::Bacteria-based anticancer therapies aim to overcome the limitations of current cancer therapy by actively targeting and efficiently removing cancer. To achieve this goal, new approaches that target and maintain bacterial drugs at sufficient concentrations during the therapeutic window are essential. Here, we examined ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.16135
更新日期:2016-06-20 00:00:00
abstract::Reperfusion as a therapeutic intervention for acute myocardial infarction-induced cardiac injury itself induces further cardiomyocyte death. β-arrestin (βarr)-biased β-adrenergic receptor (βAR) activation promotes survival signaling responses in vitro; thus, we hypothesize that this pathway can mitigate cardiomyocyte ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.26619
更新日期:2018-09-09 00:00:00
abstract::Non-invasive PET imaging of CXCR4 expression in cancer and inflammation as well as CXCR4-targeted radioligand therapy (RLT) have recently found their way into clinical research by the development of the theranostic agents [68Ga]PentixaFor (cyclo(D-Tyr1-D-[NMe]Orn2(AMBS-[68Ga]DOTA)-Arg3-Nal4-Gly5) = [68Ga]DOTA-AMBS-CPC...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45537
更新日期:2020-07-09 00:00:00
abstract::Reactive oxygen species (ROS) play a crucial role in cancer formation and development, especially cancer metastasis. However, lack of a precise tool, which could accurately distinguish specific types of ROS, restricts an in-depth study of ROS in cancer development and progression. Herein, we designed smart and versati...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.38422
更新日期:2020-02-10 00:00:00
abstract::Many drug controlled release methods have been integrated in multifunctional nanoparticles, such as pH-, redox-, temperature-, enzyme-, and light-responsive release. However, few report is associated with the ROS responsive drug controlled release. Herein, a thioketal linker-based ROS responsive drug (camptothecin con...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.14101
更新日期:2016-02-05 00:00:00
abstract::The therapeutic efficacy of fluorescence image-guided tumor surgery and photodynamic therapy (PDT) is impaired by the penetration depth limitation, low signal-to-noise ratio of traditional first near-infrared window (NIR I) fluorescence and the hypoxic tumor microenvironment. Here, a "red blood cell-based multimodal p...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.29817
更新日期:2019-01-01 00:00:00
abstract::Background: Biofilm infection caused by multidrug-resistant bacteria is difficult to eradicate by conventional therapies. Photodynamic therapy (PDT) is an effective antibacterial method for fighting against biofilm infection. However, the blocked photosensitizers outside of biofilm greatly limit the efficacy of PDT. M...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.42922
更新日期:2020-03-26 00:00:00
abstract::Rationale: To test whether novel biomarkers, such as microribonucleic acids (miRNAs), and nonstandard predictive models, such as decision tree learning, provide useful information for medical decision-making in patients on hemodialysis (HD). Methods: Samples from patients with end-stage renal disease receiving HD incl...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.46123
更新日期:2020-07-09 00:00:00
abstract::Mitochondria in cancer cells maintain a more negative membrane potential than normal cells. Mitochondria are the primary source of cellular reactive oxygen species (ROS), which are necessary for photodynamic therapy. Thus, the strategy of targeting mitochondria can maximize the photodynamic therapeutic efficiency for ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.15433
更新日期:2016-10-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) have emerged as integral components of E2F1-regulated gene regulatory networks (GRNs), but their implication in advanced or treatment-refractory malignancy is unknown. Methods: We combined high-throughput transcriptomic approaches with bioinformatics and structure modeling to search for ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44176
更新日期:2020-07-29 00:00:00
abstract::Cancer metastasis is a Gordian knot for tumor diagnosis and therapy. Many studies have demonstrated that metastatic processes are inevitably affected by the tumor microenvironment. Histopathology is used universally as the gold standard for cancer diagnosis despite the lengthy preparation process and invasiveness. Met...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.40869
更新日期:2020-01-01 00:00:00
abstract::[This retracts the article on p. 3 in vol. 1, PMID: 21547151.]. ...
journal_title:Theranostics
pub_type: 杂志文章,撤回出版物
doi:10.7150/thno.18746
更新日期:2017-01-07 00:00:00
abstract::This is the initial report of an α-based pre-targeted radioimmunotherapy (PRIT) using 225Ac and its theranostic pair, 111In. We call our novel tumor-targeting DOTA-hapten PRIT system "proteus-DOTA" or "Pr." Herein we report the first results of radiochemistry development, radiopharmacology, and stoichiometry of tumor ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.48810
更新日期:2020-09-14 00:00:00
abstract::Resistance to the chemotherapeutic drug 5'-azacytidine (5'-AZA) is a major obstacle in the treatment of patients with acute myeloid leukemia (AML). The uridine-cytidine kinase 1 (UCK1) has an established role in activating 5'-AZA and its protein level is significantly downregulated in patients resistant to the drug. H...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.36503
更新日期:2020-01-01 00:00:00
abstract::Both adjuvants and focal ablation can alter the local innate immune system and trigger a highly effective systemic response. Our goal is to determine the impact of these treatments on directly treated and distant disease and the mechanisms for the enhanced response obtained by combinatorial treatments. Methods: We com...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.25613
更新日期:2018-06-07 00:00:00
abstract::Producing keratinocyte cells (KCs) in large scale is difficult due to their slow proliferation, disabling their use as seed cells for skin regeneration and wound healing. Cell reprogramming is a promising inducer-based approach to KC production but only reaches very low cellular conversion. Here we reported a unique c...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.31526
更新日期:2019-07-09 00:00:00
abstract::Histone deacetylase 3 (HDAC3) plays a pivotal role in the repression of cartilage-specific gene expression in human chondrocytes. The aim of this study was to determine whether microRNA-193b-3p (miR-193b-3p) regulates the expression of HDAC3 during chondrogenesis and chondrocyte metabolism. Methods: miR-193b-3p expres...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.23547
更新日期:2018-04-15 00:00:00
abstract::Circulating tumor cells (CTCs) have shown promising potential as liquid biopsies that facilitate early detection, prognosis, therapeutic target selection and monitoring treatment response. CTCs in most cancer patients are low in abundance and heterogeneous in morphological and phenotypic profiles, which complicate the...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.18588
更新日期:2017-06-25 00:00:00
abstract::Photodynamic therapy (PDT) is an emerging theranostic modality for various cancers and diseases. The focus of this study was the development of tumor-targeting albumin nanoparticles containing photosensitizers for efficient PDT. To produce tumor-targeting albumin nanoparticles, the hydrophobic photosensitizer, chlorin...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno/v01p0230
更新日期:2011-04-06 00:00:00
abstract::We report a new technique to detect enzyme activity inside cells. The method based on Fluorescence Lifetime Imaging (FLIM) technology allows one to follow sensor cleavage by proteolytic enzyme caspase-3. Specifically, we use the FLIM FRET of living cells via the confocal fluorescence microscopy. A specially designed l...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3885
更新日期:2012-01-01 00:00:00
abstract::Background: By targeting the prostate-specific membrane antigen (PSMA) on prostate cancer (PCa) cells PSMA-PET/CT shows great potential in locating the site of biochemical recurrence even at low PSA (Prostate-specific antigen)-levels. Accurate imaging of PCa recurrent lymph node metastases (LNM) is crucial for metasta...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.18421
更新日期:2017-04-10 00:00:00
abstract::Immunity is the most critical and well-regulated protection to the body. Immunity is implicated in a wide range of diseases and serves as the foundation for immunotherapy. Immunotheranostics is the idea of improving immunotherapy through the organic integration of therapeutic, diagnostic, and screening technologies. T...
journal_title:Theranostics
pub_type: 社论
doi:10.7150/thno.48966
更新日期:2020-06-11 00:00:00
abstract::Rationale: Tumor microenvironment interacts with tumor cells to regulate their stemness properties through various cytokines and cytokine receptors. Previous studies revealed the possible role of interleukin 20 receptor subunit alpha (IL20RA) signaling in the progression of several types of tumors. However, its regula...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45280
更新日期:2021-01-01 00:00:00
abstract::RNAi-based strategies provide a great therapeutic potential for treatment of various human diseases including kidney disorders, but face the challenge of in vivo delivery and specific targeting. The chitosan delivery system has previously been shown to target siRNA specifically to the kidneys in mice when administered...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.7866
更新日期:2014-08-13 00:00:00
abstract::Transcription factor FOXM1 is involved in stimulating cell proliferation, enhancing DNA damage repair, promoting metastasis of cancer cells, and the inhibition of FOXM1 has been shown to prevent the initiation and progression of multiple cancers and FOXM1 is considered to be an effective target for tumor therapeutic d...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.32693
更新日期:2019-04-25 00:00:00
abstract::In ultrasound molecular imaging (USMI), ligand-functionalized microbubbles (MBs) are used to visualize vascular endothelial targets. Netrin-1 is upregulated in 60% of metastatic breast cancers and promotes tumor progression. A novel netrin-1 interference therapy requires the assessment of netrin-1 expression prior to ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.27221
更新日期:2018-10-06 00:00:00
abstract::Oral squamous cell carcinoma (OSCC), an epithelial malignancy affecting a variety of subsites in the oral cavity, is prevalent in Asia. The survival rate of OSCC patients has not improved over the past decades due to its heterogeneous etiology, genetic aberrations, and treatment outcomes. Improvement in therapeutic st...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.18551
更新日期:2017-02-26 00:00:00
abstract::Cancer vaccines have encountered their ideal personalized partner along with evidence for great breakthroughs in the identification and synthesis of neoantigens. Individual cancer neoantigen vaccines are capable of eliciting robust T-cell responses and have been demonstrated to achieve striking clinical efficacy due t...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.24387
更新日期:2018-07-30 00:00:00
abstract::Recently, self-assembling small dendrimers into supramolecular dendritic systems offers an alternative strategy to develop multifunctional nanoplatforms for biomedical applications. We herein report a dual-responsive supramolecular PEGylated dendritic system for efficient platinum-based drug delivery and near-infrared...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.15081
更新日期:2016-06-05 00:00:00
abstract::Rationale: Following an ever-increased focus on personalized medicine, there is a continuing need to develop preclinical molecular imaging modalities to guide the development and optimization of targeted therapies. Near-Infrared (NIR) Macroscopic Fluorescence Lifetime Förster Resonance Energy Transfer (MFLI-FRET) imag...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45825
更新日期:2020-08-15 00:00:00