Abstract:
:We report a new technique to detect enzyme activity inside cells. The method based on Fluorescence Lifetime Imaging (FLIM) technology allows one to follow sensor cleavage by proteolytic enzyme caspase-3. Specifically, we use the FLIM FRET of living cells via the confocal fluorescence microscopy. A specially designed lentivector pLVT with the DNA fragment of TagRFP-23-KFP was applied for transduction of A549 cell lines. Computer simulations are carried out to estimate FRET efficiency and to analyze possible steric restrictions of the reaction between the substrate TagRFP-23-KFP and caspase-3 dimer. Successful use of the fuse protein TagRFP-23-KFP to register the caspase-3 activation based on average life-time measurements is demonstrated. We show that the average life-time distribution is dramatically changed for cells with the modified morphology that is typical for apoptosis. Namely, the short-lived component at 1.8-2.1 ns completely disappears and the long-lived component appears at 2.4-2.6 ns. The latter is a fingerprint of the TagRFP molecule released after cleavage of the TagRFP-23-KFP complex by caspase-3. Analysis of life-time distributions for population of cells allows us to discriminate apoptotic and surviving cells within single frame and to peform statistical analysis of drug efficiency. This system can be adjusted for HTS by using special readers oriented on measurements of fluorescence life-time.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Savitsky AP,Rusanov AL,Zherdeva VV,Gorodnicheva TV,Khrenova MG,Nemukhin AVdoi
10.7150/thno.3885subject
Has Abstractpub_date
2012-01-01 00:00:00pages
215-26issue
2issn
1838-7640pii
thnov02p0215journal_volume
2pub_type
杂志文章相关文献
Theranostics文献大全abstract::Immuno-oncological treatment strategies that target abnormal receptor profiles of tumors are an increasingly important feature of cancer therapy. Yet, assessing receptor availability (RA) and drug-target engagement, important determinants of therapeutic efficacy, is challenging with current imaging strategies, largely...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45273
更新日期:2020-09-14 00:00:00
abstract:PURPOSE:The efficacy of pro-angiogenic therapy is difficult to evaluate with current diagnostic modalities. The objectives were to develop a non-invasive imaging strategy to define the temporal characteristics of angiogenesis and to evaluate the response to pro-angiogenic therapy in diabetic stroke mouse models. METHO...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.9525
更新日期:2014-05-25 00:00:00
abstract::Chronic kidney disease (CKD) is a serious healthcare dilemma, associated with specific changes in gut microbiota and circulating metabolome. Yet, the functional capacity of CKD microbiome and its intricate relationship with the host metabolism at different stages of disease are less understood. METHODS:Here, shotgun ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.41725
更新日期:2020-04-06 00:00:00
abstract::Sodium/iodide symporter (NIS)-mediated iodide uptake in thyroid follicular cells is the basis of clinical utilization of radioiodines. The cloning of the NIS gene enabled applications of NIS as a reporter gene in both preclinical and translational research. Non-invasive NIS imaging with radioactive iodides and iodide ...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.24997
更新日期:2018-06-24 00:00:00
abstract::[This retracts the article on p. 3 in vol. 1, PMID: 21547151.]. ...
journal_title:Theranostics
pub_type: 杂志文章,撤回出版物
doi:10.7150/thno.18746
更新日期:2017-01-07 00:00:00
abstract::Photodynamic therapy (PDT) is an emerging theranostic modality for various cancers and diseases. The focus of this study was the development of tumor-targeting albumin nanoparticles containing photosensitizers for efficient PDT. To produce tumor-targeting albumin nanoparticles, the hydrophobic photosensitizer, chlorin...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno/v01p0230
更新日期:2011-04-06 00:00:00
abstract::Because of the unparalleled efficiency and universal utility in treating a variety of disease types, siRNA agents have evolved as the future drug-of-choice. Yet, the inability of the polyanionic siRNA macromolecules to cross the cell membrane remains as the bottleneck of possible clinical applications. With the cell p...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19863
更新日期:2017-06-25 00:00:00
abstract::Developing ultrasensitive contrast agents for the early detection of malignant tumors in liver is highly demanded. Constructing hepatic tumors specific targeting probes could provide more sensitive imaging information but still faces great challenges. Here we report a novel approach for the synthesis of ultra-small Fe...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.14280
更新日期:2016-07-15 00:00:00
abstract::Folate receptor (FR) has proven to be a valuable target for chemotherapy using folic acid (FA) conjugates. However, FA-conjugated chemotherapeutics still have low therapeutic efficacy accompanied with side effects, resulting from complications such as short circulation half-life, limited tumor delivery, as well as hig...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.24382
更新日期:2018-02-16 00:00:00
abstract::Circulating tumor cells (CTCs) have shown promising potential as liquid biopsies that facilitate early detection, prognosis, therapeutic target selection and monitoring treatment response. CTCs in most cancer patients are low in abundance and heterogeneous in morphological and phenotypic profiles, which complicate the...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.18588
更新日期:2017-06-25 00:00:00
abstract::Background: Circular RNAs (circRNAs) are a new class of non-coding RNAs (ncRNAs) that are derived from exons or introns by special selective shearing. circRNAs have been shown to play critical roles in various human cancers. However, their roles in renal cell carcinoma (RCC) and the underlying mechanisms remain largel...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47239
更新日期:2020-08-29 00:00:00
abstract::Extracellular vesicles, in particular exosomes, have recently gained interest as novel drug delivery vectors due to their biological origin and inherent intercellular biomolecule delivery capability. An in-depth knowledge of their in vivo biodistribution is therefore essential. This work aimed to develop a novel, reli...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.27891
更新日期:2019-02-28 00:00:00
abstract::Recently, researchers identified a distinct vessel subtype called type H vessels that couple angiogenesis and osteogenesis. We previously found that type H vessels are reduced in ovariectomy (OVX)-induced osteoporotic mice, and preosteoclasts are able to secrete platelet-derived growth factor-BB (PDGF-BB) to stimulate...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.22144
更新日期:2018-03-28 00:00:00
abstract::In the mammalian skeletal system, osteogenesis and angiogenesis are intimately linked during bone growth and regeneration in bone modeling and during bone homeostasis in bone remodeling. Recent studies have expanded our knowledge about the molecular and cellular mechanisms responsible for coupling angiogenesis and bon...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.34126
更新日期:2020-01-01 00:00:00
abstract::Rationale: The incidence of hepatocellular carcinoma is rising worldwide. It is predicted that nearly half of the early-stage hepatocellular carcinoma (E-HCC) patients will develop recurrence. Dysregulated pH, a hallmark of E-HCC, is correlated with poor prognosis. The acidic microenvironment has been shown to promote...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.30958
更新日期:2019-03-16 00:00:00
abstract::Immunity is the most critical and well-regulated protection to the body. Immunity is implicated in a wide range of diseases and serves as the foundation for immunotherapy. Immunotheranostics is the idea of improving immunotherapy through the organic integration of therapeutic, diagnostic, and screening technologies. T...
journal_title:Theranostics
pub_type: 社论
doi:10.7150/thno.48966
更新日期:2020-06-11 00:00:00
abstract::Rationale: Busulfan is currently an indispensable anti-cancer drug, particularly for children, but the side effects on male reproduction are so serious that critical drug management is needed to minimize any negative impact. Meanwhile, alginate oligosaccharides (AOS) are natural products with many consequent advantage...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.43189
更新日期:2020-02-10 00:00:00
abstract::Constructing nanosystems that synergistically combine therapeutic and diagnostic features is of great interest to the nanomedicine community but also remains a tremendous challenge. Methods: In this work, we report novel catalytic nanoparticles composed of the enzyme catalase, encapsulated in a polymer shell and surfa...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.37054
更新日期:2019-10-17 00:00:00
abstract::Model observers play an important role in the optimization and assessment of imaging devices. In this review paper, we first discuss the basic concepts of model observers, which include the mathematical foundations and psychophysical considerations in designing both optimal observers for optimizing imaging systems and...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.5138
更新日期:2013-10-04 00:00:00
abstract::Reperfusion as a therapeutic intervention for acute myocardial infarction-induced cardiac injury itself induces further cardiomyocyte death. β-arrestin (βarr)-biased β-adrenergic receptor (βAR) activation promotes survival signaling responses in vitro; thus, we hypothesize that this pathway can mitigate cardiomyocyte ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.26619
更新日期:2018-09-09 00:00:00
abstract::Prolonged occlusion of multiple microvessels causes microvascular injury. G protein-coupled receptor 124 (GPR124) has been reported to be required for maintaining central nervous system (CNS) angiogenesis and blood-brain barrier integrity. However, the molecular mechanisms by which GPR124 regulates pericytes during is...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.34168
更新日期:2019-08-14 00:00:00
abstract::Recently, self-assembling small dendrimers into supramolecular dendritic systems offers an alternative strategy to develop multifunctional nanoplatforms for biomedical applications. We herein report a dual-responsive supramolecular PEGylated dendritic system for efficient platinum-based drug delivery and near-infrared...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.15081
更新日期:2016-06-05 00:00:00
abstract::Purpose: Cancer immunotherapy depends on a systemic immune response, but the basic underlying mechanisms are still largely unknown. Despite the very successful and widespread use of checkpoint inhibitors in the clinic, the majority of cancer patients do not benefit from this type of treatment. In this translational st...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.35989
更新日期:2020-01-01 00:00:00
abstract::Bacteria-based anticancer therapies aim to overcome the limitations of current cancer therapy by actively targeting and efficiently removing cancer. To achieve this goal, new approaches that target and maintain bacterial drugs at sufficient concentrations during the therapeutic window are essential. Here, we examined ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.16135
更新日期:2016-06-20 00:00:00
abstract::Zinc-finger proteins (ZFPs) are the largest transcription factor family in mammals, involved in the regulation of multiple physiologic processes including cell differentiation, proliferation, apoptosis and neoplastic transformation. Approximately one-third of ZFPs are Krüppel-associated box domain (KRAB)-ZFPs. Methods...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.35861
更新日期:2020-01-12 00:00:00
abstract::Compared with other diagnostic methods, ultrasound is proven to be a safe, simple, non-invasive and cost-effective imaging technique, but the resolution is not comparable to that of magnetic resonance imaging (MRI). Contrast-enhanced ultrasound employing microbubbles can gain a better resolution and is now widely used...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3464
更新日期:2012-01-01 00:00:00
abstract::Background: Lung cancer has a high mortality rate and is resistant to multiple chemotherapeutics. Natural Borneol (NB) is a monoterpenoid compound that facilitates the bioavailability of drugs. In this study, we investigated the effects of NB on chemosensitivity in the A549 human lung adenocarcinoma cell line and to e...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45861
更新日期:2020-08-13 00:00:00
abstract::Aim: Immune responsive 12/15 lipoxygenase (12/15LOX)-orchestrate biosynthesis of essential inflammation-resolution mediators during acute inflammatory response in post-myocardial infarction (MI). Lack of 12/15LOX dampens proinflammatory mediator 12-(S)-hydroxyeicosatetraenoic acid (12-(S)-HETE), improves post-MI survi...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.51183
更新日期:2021-01-01 00:00:00
abstract::Despite significant advances in cancer diagnostics and treatment, ovarian cancers (OC) continue to kill more than 150,000 women every year worldwide. Due to the relatively asymptomatic nature and the advanced stage of the disease at the time of diagnosis, OC is the most lethal gynecologic malignancy. The current treat...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.26345
更新日期:2018-07-30 00:00:00
abstract::Angiogenesis enhances cancer metastasis and progression, however, the roles of transcription regulation in angiogenesis are not fully defined. ZNF322A is an oncogenic zinc-finger transcription factor. Here, we demonstrate a new mechanism of Kras mutation-driven ZNF322A transcriptional activation and elucidate the inte...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47491
更新日期:2020-08-08 00:00:00