Abstract:
Background:MicroRNA-647 (miR-647) has been reported to repress cell tumorigenic phenotype, while the function of miR-647 in non-small cell lung cancer was obscure. Methods:The effect of miR-647 and TRAF2 on A549 and H1299 cells was explored through Methyl thiazolyl tetrazolium (MTT) assay, colony formation and cell cycle assays. Luciferase reporter assays, reverse transcription quantitative PCR (RT-qPCR) and Western blot assay were carried out to determine that TRAF2 is directly regulated by miR-647. The effect of miR-647/TRAF2 axis on p65 protein level in nucleus or total was detected by Western blot assay. Results:Here, we found that miR-647 was high expression in tumor that under argon-helium cryoablation treatment in contrast to the tumor under non argon-helium cryoablation treatment and inhibited cell proliferation of A549 and H1299 cells by inducing G1-S transition. TRAF2 was confirmed as a target of miR-647. TRAF2 overexpression partially rescued the suppressive function of miR-647 in A549 and H1299 cells. Moreover, we found that miR-647 repressed lung carcinogenesis by attenuating NF-κB pathway. Conclusion:In all, our study demonstrates that miR-647 functions as tumor suppressor via targeting and down-regulating the expression of TRAF2 and NF-κB signaling pathway in non-small cell lung cancer.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Zhang YS,Chen T,Cai YJ,Dong J,Bai F,Gao X,Tian L,Duan N,Liu Ddoi
10.2147/OTT.S159337subject
Has Abstractpub_date
2018-10-10 00:00:00pages
6777-6784issn
1178-6930pii
ott-11-6777journal_volume
11pub_type
杂志文章abstract:Introduction:The purpose of our research was to evaluate MT1JP in breast cancer. Material and Methods:For clinical purpose, tissues were collected, and a correlation analysis ofMT1JP and miRNA-214 gene expressions was conducted. Using an in vitro study, MDA-MB-231 and MCF-7 cell lines were used as research objects in ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S241503
更新日期:2020-06-04 00:00:00
abstract:BACKGROUND:The purpose of this research was to investigate the relationship between epidermal growth factor receptor (EGFR) mutations and the response to first-line chemotherapy in patients with advanced non-small cell lung cancer (NSCLC). METHODS:A total of 266 patients with stage IIIB or IV NSCLC who received platin...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S63665
更新日期:2014-07-01 00:00:00
abstract:Objective:To explore the regulatory effects of circZNF609 on proliferative and migratory capacities of gastric cancer (GC) and its underlying mechanism. Methods:Expression level of circZNF609, CDK6 and miR-483-3p in GC tissues and cells were detected qRT-PCR verification. CCK-8 and transwell assay were conducted the c...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S193031
更新日期:2019-10-04 00:00:00
abstract:Introduction:Disulfiram (DSF), a drug used in the treatment of alcoholism since 1948, has been shown to have antitumor properties in various tumor types possibly due to the induction of a type cell death, ferroptosis, and the sensitization of cells to chemo- and radiotherapy. In this study, we explored the antitumor pr...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S272312
更新日期:2020-10-20 00:00:00
abstract:Objective:The objective of this study was to investigate the safety of robotic stereotactic body radiation therapy (SBRT) for hepatocellular carcinoma (HCC) patients and its related factors. Methods:A total of 74 HCC patients with Child-Turcotte-Pugh (CTP) Class A were included in a multi-institutional, single-arm Pha...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S142025
更新日期:2017-11-06 00:00:00
abstract:PURPOSE:This study aimed to explore the molecular mechanisms associated with bisphosphonate (BP)-related osteonecrosis of the jaw (ONJ) in patients with multiple myeloma (MM). METHODS:The gene expression profile GSE7116 was downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) fro...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S88463
更新日期:2015-09-22 00:00:00
abstract:Purpose:B7 homologue 6 (B7-H6) has been found at an up-regulated level in multiple cancer cells and identified to be positively correlated with inferior clinical features. In non-Hodgkin lymphoma (NHL), however, the roles of B7-H6 and the underlying mechanism of action remain unclear. Through in vivo and in vitro exper...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S257512
更新日期:2020-06-18 00:00:00
abstract::Background/Aims: MiR-145 and Smad2 have been widely reported in the development and progression of human malignancies. In the present study, we investigated the correlation between miR-145 and Smad2 in human glioblastoma multiforme (GBM). Methods: The epithelial-mesenchymal transition (EMT) biomarkers and Smad2 were a...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S202129
更新日期:2019-04-23 00:00:00
abstract:BACKGROUND:Paxillin is implicated in tumorigenesis, progression and aggressive phenotypes of various malignancies, highlighting its functions in cellular adhesion, migration and survival. However, the roles of paxillin in human gliomas remain unclear. The aim of this study was to evaluate the clinical implication of pa...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S114229
更新日期:2016-11-09 00:00:00
abstract:Background:Investigating the roles of lncRNA prostate cancer-associated transcript 6 (PCAT6) in modulating the growth and aggressiveness of non-small-cell lung carcinoma (NSCLC) cell. Method:The levels of PCAT6 in NSCLC tissues and cell lines were determined by quantitative real-time PCR assay. MTT as well as colony f...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S178597
更新日期:2018-11-01 00:00:00
abstract:Background and aim:Standard chemotherapy has limited clinical efficacy in patients with esophageal cancer and there is a significant and unmet clinical need for effective treatment options for these patients. The aim of this study was to compare the clinical efficacy of the novel, targeted drug apatinib combined with d...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S191736
更新日期:2019-04-08 00:00:00
abstract:Background:Pulmonary carcinosarcomas (PCSs) are a heterogeneous group of non-small-cell lung carcinomas (NSCLCs) with aggressiveness and a poor prognosis. Although genetic mutations of some common lung cancer subtypes have been extensively studied, the molecular characteristics of PCSs and the existence of abnormal tar...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S264617
更新日期:2020-08-10 00:00:00
abstract:Background and Objectives:Non-small cell lung cancer (NSCLC) patients with an epidermal growth factor receptor (EGFR) mutation demonstrate only a median progression-free survival (PFS) of 8 to 10 months and undergo EGFR tyrosine kinase inhibitors (EGFR-TKIs) therapy. For decades, bilirubin has been reported to be assoc...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S266477
更新日期:2020-10-29 00:00:00
abstract:OBJECTIVE:To investigate the effects of microRNA-122 (miR-122) on proliferation, migration, and invasion in human hepatocellular carcinoma (HCC) cells by activating epithelial-mesenchymal transition (EMT) pathways. METHODS:miR-122 mimics, miR-122 inhibitors, relevant control oligonucleotides, and Wnt1 were transfected...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S92378
更新日期:2016-04-06 00:00:00
abstract::Orbital metastasis as the initial presentation of lung adenocarcinoma is very rare, and so the lack of knowledge about this phenomenon can easily result in misdiagnosis, either as a orbital primary tumor or benign lesion. Here, we report a rare case in which the orbital symptom appeared first without any pulmonary man...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S99583
更新日期:2016-05-09 00:00:00
abstract::Despite multimodal therapeutic treatments of osteosarcoma (OS), some patients develop resistance to currently available regimens and eventually end up with recurrent or metastatic outcomes. Many attempts have been made to discover effective drugs for improving outcome; however, due to the heterogeneity of the disease,...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S119993
更新日期:2017-02-01 00:00:00
abstract:BACKGROUND:Mast cells (MCs) can stimulate angiogenesis, releasing several proangiogenic cytokines stored in their cytoplasm. In particular MCs can release tryptase, a potent in vivo and in vitro proangiogenic factor via proteinase-activated receptor-2 (PAR-2) activation and mitogen-activated protein kinase phosphorylat...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S105368
更新日期:2016-07-21 00:00:00
abstract:Background/Aims:The effects of lncRNA-NORAD/mir-520a-3p on proliferation and invasion of non-small cell lung cancer (NSCLC) were studied, and its potential molecular mechanism was discussed. Methods:qRT-PCR was used to detect the expression of lncRNA NORAD and miR-520a-3p in non-small cell lung cancer tissues and cell...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S230954
更新日期:2020-02-19 00:00:00
abstract:Background:Recent studies showed that aberrant expression of miRNAs causes tumor-suppressing or promoting effects in various cancers including gastric cancer (GC). Our previous studies showed that lots of miRNAs and mRNA expressed differentially in GC and normal tissues. However, the critical miRNAs and mRNA need to be...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S208024
更新日期:2020-03-03 00:00:00
abstract:BACKGROUND:Bevacizumab plus taxane chemotherapy improves progression-free survival (PFS) versus taxane monotherapy in the first-line treatment of HER2-negative metastatic breast cancer (MBC) and appears promising in the second-line setting. This retrospective analysis evaluated the efficacy and safety of this combinati...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S70654
更新日期:2014-11-27 00:00:00
abstract::Normal astrocytes are more resistant to radiation than glioma cells. Radiation-resistant glioma cells and normal astrocytes usuallly share similar mechanisms of radioresistance. Investigation of the underlying mechanisms of differential radiation response between normal astrocytes and glioma cells is thus significant ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S144002
更新日期:2017-12-04 00:00:00
abstract:Introduction:Odd-skipped related transcription factor 1 (OSR1) is a newly identified tumor suppressor in many tumor types. However, the role and mechanism of OSR1 in colon adenocarcinoma (COAD) remain unknown. Methods:OSR1 expression was detected in COAD tissues and cells. COAD cells with OSR1 overexpression or knockd...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S242386
更新日期:2020-04-24 00:00:00
abstract::During the past decade, immunotherapy targeting immune checkpoints has become an important component of the treatment paradigm for numerous malignancies, especially PD-1/PD-L1 blockade which was demonstrated to rejuvenate disabled T cells in cancer patients to achieve long-term remissions. However, the clinical outcom...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S221340
更新日期:2019-10-15 00:00:00
abstract:Purpose:This study aimed to investigate the regulatory roles of estrogen receptor beta (ERβ) on gastric cancer (GC) cells, and reveal the potential mechanisms relating to nuclear factor-kappa B (NF-κB) signaling. Methods:GC cell lines SGC7901 and MKN45 were transfected with pEGFP-C1-ERβ to overexpress ERβ, and treated...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,收录出版
doi:10.2147/OTT.S214529
更新日期:2019-11-05 00:00:00
abstract::miRNAs (miRs) are short RNA molecules that are involved in the posttranscriptional regulation of mRNA. The roles of miRs in tumor pathogenesis have only recently become a focus of research. It is becoming increasingly clear that miRs are important regulators of apoptosis, proliferation, invasion, and metastasis in can...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S52730
更新日期:2013-11-18 00:00:00
abstract:Background:Abnormal miR-27a-3p expression has been frequently reported in several types of human cancer and contributes to tumor progression. However, the role and potential molecular mechanism of miR-27a-3p in the progression of pancreatic carcinoma have not been clarified. Materials and methods:The expression of miR...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S220621
更新日期:2019-12-19 00:00:00
abstract:Background:LncRNA LINC00461 has been reported to play crucial regulatory roles in a variety of biological processes, including cell migration, cell invasion and cancer progression. However, its biological role in colorectal cancer (CRC) is completely unknown. The aim of our study was to explore the function of LINC0046...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S228798
更新日期:2019-12-17 00:00:00
abstract:OBJECTIVE:Despite advances in the early diagnosis of gastrointestinal (GI) cancers, these cancers are often being detected rather late in their course. Emerging published data on the accuracy of dickkopf-1 (DKK1) for diagnosing GI cancers are inconsistent. The purpose of this systematic review and meta-analysis was to ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S93152
更新日期:2015-10-22 00:00:00
abstract::High-mobility group AT-hook 2 (HMGA2) is involved in a wide spectrum of biological processes and is upregulated in several tumors, but its role in renal carcinoma remains unclear. The aim of this study was to examine the expression of HMGA2 and its relationship to the overall survival (OS) of patients with non-metasta...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S116953
更新日期:2016-11-22 00:00:00
abstract:Background:Non-small-cell lung cancer (NSCLC) is the major leading cause of cancer-related death around the world. The resistance to chemotherapy limits the effects of clinical treatment. The aim of this study was to identify novel mechanisms involved in NSCLC chemoresistance. Materials and methods:We explored the pub...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S175606
更新日期:2018-09-26 00:00:00