Abstract:
Background:Erlotinib is a commonly used molecular-targeted drug for the treatment of tongue cancer. However, the development of acquired resistance to erlotinib hampers its therapeutic use. Materials and methods:To analyze the erlotinib resistance, long-term and short term survival assay were used to compare the resistance between parental and resistant tongue cancer cells. Flow cytometry, Hochest staining and western blot were used to analyze the apoptosis among the cells. Moreover, Transwell and wound healing assay were used to compare the invasion ability of the cells. To deeply explore the drug resistance in vivo, orthotopic tumor studies were applied. Finally, to explain the mechanism of c-met in erlotinib resistance, shRNA against c-met was used to down-regulate the expression of c-met. And SU11274 also used in orthotopic model. Results:We established erlotinib-resistant human tongue cancer cell line by chronic exposure of TCA-8113 cells to increasing concentrations of erlotinib and determined the role of c-MET and EGFR in the development of acquired resistance. We found a significant increase in the phosphorylation of c-MET and an obvious decrease of the phosphorylation of EGFR in erlotinib-resistant cells. Our results also revealed that inhibition of c-MET alone with SU11274 exerted an inhibitory effect on the proliferation of erlotinib-resistant cells in the short term; however, it failed to sustain the inhibitory effect in the long term. Simultaneous inhibition of c-MET and EGFR significantly inhibited the proliferation of erlotinib-resistant cells in both a short and long period. Furthermore, we explored the underlying mechanism and found that treatment of erlotinib-resistant cells with SU11274 or shRNA against c-MET induced the phosphorylation of EGFR. Moreover, our results demonstrated that simultaneous inhibition of c-MET and EGFR significantly inhibited the migration and invasion of erlotinib-resistant cells. Conclusion:Taken together, our results suggested that c-MET is involved in acquired drug resistance to erlotinib and that cotargeting of EGFR and c-MET could overcome acquired resistance to erlotinib and inhibit the invasion and metastasis of erlotinib-resistant cells.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Huang K,Liu Ddoi
10.2147/OTT.S167936subject
Has Abstractpub_date
2018-09-05 00:00:00pages
5499-5508issn
1178-6930pii
ott-11-5499journal_volume
11pub_type
杂志文章abstract::Follicular dendritic cell sarcoma (FDCS) is a rare malignant tumor recognized in recent years. It accounts for only 0.4% of soft-tissue sarcomas, and its underlying causes are largely unknown. A correct diagnosis can be difficult to make. Diagnosis of FDCS depends on the combined clinical examination, histopathologic ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S86502
更新日期:2015-07-24 00:00:00
abstract::Tamoxifen was the first targeted anticancer agent for breast cancer patients and its effects on reduction of breast cancer events and improvement in overall survival are undisputed. Hence, it has long been considered an essential part of patient care. Recent results of several large adjuvant hormonal trials evaluating...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S10155
更新日期:2010-12-17 00:00:00
abstract::Esophageal carcinoma (EC) is a lethal disease with high morbidity and mortality worldwide, and the incidence has been increasing in recent years. Although the diagnosis and treatment of EC have improved considerably, EC has rapidly progressed in the clinical setting and has a poor prognosis for its metastasis and recu...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S103179
更新日期:2016-04-19 00:00:00
abstract:Background:Although several long non-coding RNAs (lncRNAs) have been found to be involved in gastric cancer tumorigenesis, the more comprehensive contributions of lncRNAs to gastric cancer require further investigation. Here, we identify a cytoplasmic lncRNA, LINC01485, which promotes tumor growth and migration in gast...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S257151
更新日期:2020-08-21 00:00:00
abstract:Background:Long non-coding RNAs (lncRNAs) have been verified to have a vital role in the progression of glioblastoma multiforme (GBM). Our research was about to identify the potential lncRNAs which was closely associated with the pathogenesis and prognosis of glioblastoma multiforme. Methods:All RNA sequence profiling...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S235951
更新日期:2020-02-04 00:00:00
abstract::Background: Non-small-cell lung cancer (NSCLC) remains the leading cause of cancer morbidity and mortality worldwide. In the present study, we identified novel biomarkers associated with the pathogenesis of NSCLC aiming to provide new diagnostic and therapeutic approaches for NSCLC. Methods: The microarray datasets of...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S198621
更新日期:2019-05-13 00:00:00
abstract:BACKGROUND:Mast cells (MCs) can stimulate angiogenesis, releasing several proangiogenic cytokines stored in their cytoplasm. In particular MCs can release tryptase, a potent in vivo and in vitro proangiogenic factor via proteinase-activated receptor-2 (PAR-2) activation and mitogen-activated protein kinase phosphorylat...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S105368
更新日期:2016-07-21 00:00:00
abstract::Prostate cancer is the leading malignancy and the second most common cause of cancer-related death in men. Despite high cure rates with surgery and/or radiation, 30%-40% of patients eventually develop advanced cancer. Docetaxel is one of the most effective and well established chemotherapeutic agents for prostate canc...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S79105
更新日期:2015-04-10 00:00:00
abstract:Background:miR520a-3p has previously had its antitumorigenic role in various types of cancers revealed, and been predicted as a posttranscriptional regulator of the NFκB-subunit RELA gene. Thus, miR520a-3p could function in carcinogenesis through suppressing RELA. Methods:Expression of miR520a-3p and RELA mRNA was qua...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S208889
更新日期:2019-08-14 00:00:00
abstract:BACKGROUND:The purpose of this study was to determine the efficacy of sorafenib in preventing and treating tumor recurrence after liver transplantation in patients with primary hepatic carcinoma exceeding the Milan criteria. METHODS:Thirty patients with primary hepatic carcinoma exceeding the Milan criteria underwent ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S31387
更新日期:2012-01-01 00:00:00
abstract:Introduction:Previous trials demonstrated that anti-angiogenesis or anti-programmed death protein 1 (PD-1) monotherapy showed unsatisfied effect in advanced hepatocellular carcinoma (HCC). No study existed that focus on the effects of camrelizumab and apatinib ("C+A") combination therapy for HCC patients with the locat...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S286169
更新日期:2020-12-09 00:00:00
abstract::[This corrects the article DOI: 10.2147/OTT.S196910.]. ...
journal_title:OncoTargets and therapy
pub_type: 已发布勘误
doi:10.2147/OTT.S229714
更新日期:2019-09-19 00:00:00
abstract::The identification of molecular genetic biomarkers considerably increased our current understanding of glioma genesis, prognostic evaluation, and treatment planning. In glioblastoma, the most malignant intrinsic brain tumor entity in adults, the promoter methylation status of the gene encoding for the repair enzyme O6...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S50208
更新日期:2013-09-27 00:00:00
abstract:Background:This study was designed to research the potential function of lncRNA ANRIL in osteosarcoma (OS). Materials and methods:Quantitative real-time PCR, cell counting kit-8, wound healing assay, Transwell assay, flow cytometric analysis, caspase activity analysis, and Western blot were carried out. Results:ANRIL...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S170293
更新日期:2018-08-14 00:00:00
abstract:Background:Previous reports show that SIRT6 serves as a critical modulator of the development of multiple malignancies as well as other disorders. However, its role in nasopharyngeal carcinoma (NPC) is unknown. Thus, we elucidated the effects of SIRT6 on the survival of NPC cells, and modulation of cell death. Methods...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S179866
更新日期:2018-11-01 00:00:00
abstract:Background:A biosynthetic ginsenoside, 3-O-β-D-glucopyranosyl-12-O-β-D-glucopyranosyl-dammar-24-ene-3β, 12β, 20S-triol (C3C12PPD), showed antitumor activity against many tumor cells in vitro, especially had better anti-lung cancer activity than Rg3 in vitro and in vivo. However, the effects and molecular mechanisms of ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S217039
更新日期:2019-09-09 00:00:00
abstract:Purpose:Sorafenib has revolutionized treatment of hepatocellular carcinoma (HCC), but its efficacy is limited by drug resistance. Autophagy is the process by which cellular components are transported to lysosomes for degradation, which promotes energy production and production of macromolecular precursors. Studies have...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S247655
更新日期:2020-04-28 00:00:00
abstract:Objective:We aimed to determine the safety and efficacy of irreversible electroporation (IRE) combined with chemotherapy for unresectable pancreatic carcinoma (stage III/IV). Methods:We prospectively enrolled 54 patients (30 men; median age 61.0 years; range 41-73 years) undergoing IRE with or without chemotherapy for...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S186721
更新日期:2019-02-18 00:00:00
abstract:Background:Nasopharyngeal carcinoma (NPC) is a common malignant cancer that is distributed particularly in Southeastern Asia. Previous studies have manifested that long noncoding RNA urothelial carcinoma associated 1 (UCA1) was involved in NPC tumorigenesis and metastasis. However, the biological mechanism of UCA1 for ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S215819
更新日期:2019-10-11 00:00:00
abstract:Introduction:Dynamin 3 (DNM3) is a large GTPase that possesses mechanochemical properties and has been shown to be involved in malignancies. However, most studies about DNM3 are observational, and knowledge of the precise molecular mechanism of DNM3 remains limited. Materials and methods:We constructed a PCDH-CMV-MCS-...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S176388
更新日期:2018-10-09 00:00:00
abstract::Gastrointestinal stromal tumor (GIST) is an uncommon mesenchymal tumor, and has been shown to be associated with synchronous or metachronous second malignancies. Rare cases of coincident GIST and non-Hodgkin lymphomas (NHL) have been reported previously. Here, we report two cases of GIST and coincident primary breast ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S159843
更新日期:2018-12-10 00:00:00
abstract:Objective:Cumulative data from clinical trials suggest that Lin28 may contribute to poor survival in breast cancer patients. The purpose of this study was to investigate the relationship between Lin28 expression and breast cancer patients' clinicopathological parameters. Methods:Data from a total of 291 breast cancer ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S190328
更新日期:2019-02-08 00:00:00
abstract::Erastin was initially discovered as a small molecule compound that selectively kills tumor cells expressing ST and RASV12 and was later widely investigated as an inducer of ferroptosis. Ferroptosis is a recently discovered form of cell death caused by peroxidation induced by the accumulation of intracellular lipid rea...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S254995
更新日期:2020-06-11 00:00:00
abstract::Huaier (Trametes robiniophila Murr), a Chinese traditional herb of medicine, has demonstrated promising curative effects in clinical treatment for various tumors. There are documented experiments showing the biological functions of Huaier with its antineoplastic molecular mechanisms: restraining proliferation and meta...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S281328
更新日期:2020-12-08 00:00:00
abstract:BACKGROUND:Several studies have reported an association between the A23G polymorphism (rs 1800975) in the xeroderma pigmentosum group A (XPA) gene and risk of digestive system cancers. However, the results are inconsistent. In this study, we performed a meta-analysis to assess the association between XPA A23G polymorph...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S75767
更新日期:2015-02-05 00:00:00
abstract:BACKGROUND:Laminin gamma2 (Ln-γ2) chain, a distinctive subunit of heterotrimeric laminin-332, is frequently upregulated in carcinomas and is of great importance in cell migration and invasion. Despite this, the status of circulating Ln-γ2 in lung cancer patients is still uncertain. PATIENTS AND METHODS:In this retrosp...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S105732
更新日期:2016-07-07 00:00:00
abstract:Purpose:Gastric carcinoma is the second most frequently diagnosed cancer and leading cause of cancer death in China. As a new generation of cancer therapeutic drug, CL-6, a curcumin derivative, shows better bioavailability than curcumin, which has shown anticancer effects in gastric cancer (GC). However, whether CL-6 s...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S196914
更新日期:2019-03-27 00:00:00
abstract::Major advances in the understanding of the pathophysiology of melanoma have led to a new era of melanoma treatment with targeted therapy and immunotherapies. Since 2011, four new classes of medications with unique mechanisms of action have been approved, which allow melanoma to be treated at many different stages in i...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S171693
更新日期:2018-12-14 00:00:00
abstract:BACKGROUND:The purpose of this study was to evaluate clinical outcomes of conventional fractionated helical tomotherapy in patients with small to medium hepatocellular carcinomas (HCCs) without portal vein tumor thrombosis. METHODS:Patients with up to four HCC lesions not treatable by surgery or percutaneous ablative ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S69618
更新日期:2014-09-26 00:00:00
abstract:Purpose:Radioresistance is a vital obstacle for the prognosis of human nasopharyngeal carcinoma (NPC), but the underlying mechanism is still unknown. Here, we explored the role of the NRF2/KEAP1 pathway in radioresistance of NPC cell lines. Materials and Methods:We selected NPC cell lines CNE-1 and CNE-2, treated them...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S260169
更新日期:2020-09-11 00:00:00