SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene.

Abstract:

:We found that a single nucleotide polymorphism (SNP) in the nucleoprotein gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a patient interfered with detection in a widely used commercial assay. Some 0.2% of the isolates in the EpiCoV database contain this SNP. Although SARS-CoV-2 was still detected by the other probe in the assay, this underlines the necessity of targeting two independent essential regions of a pathogen for reliable detection.

journal_name

Euro Surveill

authors

Ziegler K,Steininger P,Ziegler R,Steinmann J,Korn K,Ensser A

doi

10.2807/1560-7917.ES.2020.25.39.2001650

subject

Has Abstract

pub_date

2020-10-01 00:00:00

issue

39

eissn

1025-496X

issn

1560-7917

journal_volume

25

pub_type

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