Abstract：
Purpose:To quantify the effect of silicone hydrogel crosslink density on the adhesion at corneal epithelial cells/silicone hydrogel contact lens interface. Methods:A custom-built rheometer, referred to as the live cell monolayer rheometer, was used to measure the adhesive strengths between corneal epithelial cell monolayers and silicone hydrogel lens surfaces. The resulting stress relaxations of senofilcon A-derived silicone hydrogel materials with varying crosslinking densities and delefilcon A were tested. Senofilcon A-like materials labeled L1, L2, L3, L4, and L5 contained crosslinker concentrations of 1.2, 1.35, 1.5, 1.65, and 1.8 wt%, respectively. The residual modulus measured from the live cell monolayer rheometer provided a direct indication of adhesive attachment. Results:Within the senofilcon-derived series, the adhesive strength shows a surprising minimum with respect to crosslink density. Specifically, L1 (1.20%) has the highest adhesive strength of 39.5 ± 11.2 Pa. The adhesive strength diminishes to a minimum of 11.2 ± 2.1 Pa for L3, whereafter it increases to 14.5 ± 2.5 Pa and 18.1 ± 5.1 Pa for L4 and L5, respectively. The delefilcon A lens exhibits a comparable adhesive strength of 27.8 ± 6.3 Pa to L1. Conclusions:These results demonstrated that increasing the crosslink density has a nonmonotonic influence on the adherence of lenses to mucin-expressing corneal epithelial cells, which suggests a competition mechanism at the cell/lens interface. Translational Relevance:Because the adhesiveness of contact lenses to ocular tissues may impact the comfort level for lens wearers and affect ease of removal, this study suggests that lens adhesion can be optimized through the control of crosslink density.
journal_name
Transl Vis Sci Technoljournal_title
Translational vision science & technologyauthors
Liu C,Scales CW,Fuller GGdoi
10.1167/tvst.9.6.3subject
Has Abstractpub_date
2020-05-11 00:00:00pages
3issue
6issn
2164-2591pii
TVST-19-2046journal_volume
9pub_type
杂志文章abstract：Purpose:To investigate variation and determinants of macular layers, peripapillary retinal nerve fiber layer (pRNFL) and Bruch's membrane opening-minimum rim width (BMO-MRW) in the general population. Methods:In 1306 participants, we performed spectral domain optical coherence tomography (SD-OCT) scans of the macula, ...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.8.3.34
更新日期：2019-06-04 00:00:00
abstract：Purpose:Glaucoma remains a leading cause of irreversible blindness worldwide. Animal glaucoma models replicate high intraocular pressure, a risk factor for glaucoma, to induce retinal ganglion cell (RGC) degeneration. We describe an inducible, magnetic bead model in the Brown Norway rat in which we are able to determin...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.10.1.21
更新日期：2021-01-12 00:00:00
abstract：Purpose:To determine the effectiveness of intravitreal aflibercept (IVA) on the retinal function in eyes with diabetic macular edema (DME). Methods:Twenty-five eyes of 25 patients with DME were treated with three consecutive monthly IVA injections. The retinal sensitivities (RS) within the central 10° were determined ...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.9.11.2
更新日期：2020-10-06 00:00:00
abstract：PURPOSE:We directly demonstrated the revascularization in a free retinal pigment epithelium (RPE)-choroid graft with direct blood flow detection by experimental phase-resolved Doppler optical coherence tomography (PRD-OCT). METHODS:Seven patients with age-related macular degeneration underwent an RPE-choroid graft tra...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.4.1.2
更新日期：2015-01-13 00:00:00
abstract：Purpose:Semiautomated software applications derive quantitative retinal vascular parameters from fundus camera images. However, the extent of agreement between measurements from different applications is unclear. We evaluate the agreement between retinal measures from two software applications, the Singapore "I" Vessel...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.2.12
更新日期：2018-03-23 00:00:00
abstract：PURPOSE:To investigate whether tear hyperosmolarity, a feature of dry eye disease (DED), affects central corneal thickness (CCT), corneal light reflectivity, and/or tear film reflectivity. METHODS:This prospective, cross-sectional study involved 48 participants (38 with hyperosmolar tears and 10 controls with normo-os...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.6.3.6
更新日期：2017-05-22 00:00:00
abstract：Purposes:To investigate the limbal structure using anterior segment optical coherence tomography (AS-OCT) and compare the difference between a Chinese Han population and a Caucasian population. Methods:Sixty healthy Chinese Han subjects (109 eyes, Chinese group) and 32 healthy Caucasian subjects (51 eyes, Caucasian gr...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.4.12
更新日期：2018-08-07 00:00:00
abstract：PURPOSE:To determine the efficacy and safety of repeated intravitreal and subconjunctival administrations of sirolimus in patients with noninfectious uveitis at 1 year in the Sirolimus as a Therapeutic Approach UVEitis (SAVE) Study. METHODS:Open-label, prospective, and randomized interventional clinical trial in which...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.4.2.4
更新日期：2015-03-10 00:00:00
abstract：Purpose:The aim of the present study was to analyze quantitative optical coherence tomography (OCT) and OCT angiography (OCTA) parameters to identify clinically relevant cutoff values able to detect clinically different Stargardt's disease (STGD) subgroups. Methods:Consecutive STGD patients were recruited and underwen...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.8.6.26
更新日期：2019-12-05 00:00:00
abstract：Purpose:To explore the difference of the default mode network (DMN) in patients with retinal detachment (RD) by the study of the resting state functional connectivity (rs-FC). Methods:A total of 30 patients with RD (16 men, 14 women) and 30 similarly matched normal controls (NCs) were examined and recorded with rs-fMR...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.6.15
更新日期：2018-11-30 00:00:00
abstract：Purpose:To evaluate the effects of vascular endothelial growth factor-A (VEGF-A) gene editing in human retinal pigment epithelial (RPE) cells and human Muller cells, which are the main VEGF-A producing cells in the eye. Methods:CRISPR-Cas9 ribonucleoprotein was used to target exon 1 in VEGF-A gene. Lipofectamine CRISP...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.9.8.23
更新日期：2020-07-14 00:00:00
abstract：Purpose:To investigate reproducible surgical techniques to optimize the long-term safety of liquid crystal polymer (LCP)-based retinal prosthesis implantation. Methods:An LCP-based retinal prosthesis is fabricated monolithically on a single-body LCP substrate with all components, including the package and electrode ar...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.4.17
更新日期：2018-08-24 00:00:00
abstract：Purpose:We quantified the interrelationship between retinal blood vessel (BV) anatomical variation, spherical equivalent (SE) of refractive error, and functional diagnostic parameters in glaucoma to identify optimal parameters for the improvement of optical coherence tomography (OCT) retinal nerve fiber layer thickness...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.1.4
更新日期：2018-01-18 00:00:00
abstract：:[This corrects the article DOI: 10.1167/tvst.8.2.3.]. ...
journal_title：Translational vision science & technology
pub_type： 杂志文章,已发布勘误
doi：10.1167/tvst.8.3.9
更新日期：2019-05-02 00:00:00
abstract：Purpose:We present a new method for analyzing relative corneal refractive power (RCRP) in children undergoing orthokeratology and explore its potential association to effective myopic control. Methods:A total of 55 children aged 8 to 12 years participated in the study. Axial growth was calculated as the difference in ...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.6.17
更新日期：2018-11-30 00:00:00
abstract：PURPOSE:To examine the protective effects of antioxidants in cultured trabecular meshwork (TM) cells exposed to oxidative stress. METHODS:Primary porcine TM cells were pretreated with 50 μM resveratrol, 0.2 mM urate, 1 mM ascorbate, 1 mM reduced glutathione (rGSH), or 1 mM ρ-coumarate followed by exposure to hydrogen ...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.1.1.4
更新日期：2012-05-31 00:00:00
abstract：:[This corrects the article on p. 4 in vol. 7.]. ...
journal_title：Translational vision science & technology
pub_type： 杂志文章,已发布勘误
doi：10.1167/tvst.7.2.24
更新日期：2018-04-23 00:00:00
abstract：Purpose:To investigate the molecular components of the vitreous in order to better understand retinal physiology and disease. Methods:Vitreous was acquired from patients undergoing vitrectomy for macular hole and/or epiretinal membrane, postmortem donors, and C57BL/6J mice. Unbiased proteomic analysis was performed vi...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.3.6
更新日期：2018-05-14 00:00:00
abstract：Purpose:Gelatinous drop-like corneal dystrophy (GDLD) is a rare autosomal recessive corneal dystrophy that causes severe vision loss. Because of its poor prognosis, there is a demand for novel treatments for GDLD. Here, we establish a new in vitro disease model of GDLD based on immortalized human corneal epithelial (HC...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.6.30
更新日期：2018-12-21 00:00:00
abstract：Purpose:Accommodating intraocular lenses (A-IOLs) require capturing the ciliary muscle forces. Prior work demonstrated strong photo-initiated bonding between strips of capsular bag and poly(2-hydroxyethyl methacrylate); (pHEMA) polymer in an extraocular setting. We demonstrate that photobonding can be achieved intraocu...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.5.27
更新日期：2018-10-11 00:00:00
abstract：PURPOSE:A novel glaucoma drainage device (GDD) with local drug delivery (LDD) system was created and characterized for safety and effectiveness after implantation into the suprachoroidal space (SCS) of rabbit eyes. METHODS:Thin films of two different polymers, Poly(3-hydroxybutyrate) (P(3HB)) and Poly(4-hydroxybutyrat...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.4.3.14
更新日期：2015-06-30 00:00:00
abstract：PURPOSE:We performed video-oculography to evaluate vergence eye movement abnormalities in students diagnosed clinically with vergence disorders. We tested the efficiency of a novel rehabilitation method and evaluated its benefits with video-oculography cross-correlated with clinical tests and symptomatology. METHODS:A...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.5.2.8
更新日期：2016-03-11 00:00:00
abstract：PURPOSE:To determine the active ingredient in tea tree oil (TTO) responsible for its reported killing effect on Demodex mites, the most common ectoparasite found in the human skin extending to the eye. METHODS:Using a reported in vitro killing assay to measure the survival time of adult Demodex folliculorum up to 150 ...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.2.7.2
更新日期：2013-11-01 00:00:00
abstract：PURPOSE:We evaluated the efficacy of column scatter plots to describe genotype-phenotype correlations in a Japanese cohort with retinitis pigmentosa (RP). METHODS:Clinical records of 121 patients with RP with identified causative mutations were reviewed. Visual acuity, central and peripheral visual fields, electroreti...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.5.2.4
更新日期：2016-03-04 00:00:00
abstract：Purpose:Noninvasive keratograph break up times (NIKBUTs) are preferred to dye-based methods to evaluate tear stability in translational medicine. We analyzed the NIKBUTs in different regions of the precorneal tear by using a common imaging technology and explored potential correlations with clinical parameters. Method...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.8.5.30
更新日期：2019-10-17 00:00:00
abstract：Purpose:To investigate whether plasma rich in growth factors (PRGF) eye drops maintain their biological potential after a freeze drying process. The addition of a lyoprotectant like trehalose was also evaluated. Methods:Blood from three healthy donors was collected to obtain eye drops by PRGF technology. The resultant...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.9.7.35
更新日期：2020-06-25 00:00:00
abstract：PURPOSE:Optical coherence tomography angiography (OCT-A) allows noninvasive visualization of retinal vessels in vivo. OCT-A was used to characterize the vascular network of the mouse retina and was compared with fluorescein angiography (FA) and histology. METHODS:In the present study, OCT-A based on a Heidelberg Engin...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.5.4.11
更新日期：2016-08-18 00:00:00
abstract：Purpose:Congenital stationary night blindness 2A (CSNB2A) is a genetic retinal disorder characterized by poor visual acuity, nystagmus, strabismus, and other signs of retinal dysfunction resulting from mutations in Cacna1f-the gene coding for the pore-forming subunit of the calcium channel CaV1.4. Mouse models of CSNB2...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.9.11.19
更新日期：2020-10-14 00:00:00
abstract：Purpose:To compare the repeatability and reproducibility of axial and lateral retinal measurements using handheld optical coherence tomography (OCT) systems and a tabletop OCT system. Methods:Graders measured central foveal thickness (CFT), optic nerve-to-fovea distance (OFD), and retinal nerve fiber layer (RNFL) thic...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.9.11.25
更新日期：2020-10-21 00:00:00
abstract：Purpose:Myopia is rising in prevalence in many locations, and there is evidence that outdoor light exposure is a major environmental factor playing a role in myopia development. This study examined the patterns of daily light exposure in similarly aged children from two geographic locations (Australia and Singapore) kn...
journal_title：Translational vision science & technology
pub_type： 杂志文章
doi：10.1167/tvst.7.3.8
更新日期：2018-05-29 00:00:00
