Abstract:
BACKGROUND:Immunization with attenuated malaria sporozoites protects humans from experimental malaria challenge by mosquito bite. Protection in humans is strongly correlated with the production of T cells targeting a heterogeneous population of pre-erythrocyte antigen proteoforms, including liver stage antigens. Currently, few T cell epitopes derived from Plasmodium falciparum, the major aetiologic agent of malaria in humans are known. METHODS:In this study both in vitro and in vivo malaria liver stage models were used to sequence host and pathogen proteoforms. Proteoforms from these diverse models were subjected to mild acid elution (of soluble forms), multi-dimensional fractionation, tandem mass spectrometry, and top-down bioinformatics analysis to identify proteoforms in their intact state. RESULTS:These results identify a group of host and malaria liver stage proteoforms that meet a 5% false discovery rate threshold. CONCLUSIONS:This work provides proof-of-concept for the validity of this mass spectrometry/bioinformatic approach for future studies seeking to reveal malaria liver stage antigens towards vaccine development.
journal_name
Malar Jjournal_title
Malaria journalauthors
Winer B,Edgel KA,Zou X,Sellau J,Hadiwidjojo S,Garver LS,McDonough CE,Kelleher NL,Thomas PM,Villasante E,Ploss A,Gerbasi VRdoi
10.1186/s12936-019-3093-3subject
Has Abstractpub_date
2020-01-07 00:00:00pages
10issue
1issn
1475-2875pii
10.1186/s12936-019-3093-3journal_volume
19pub_type
杂志文章相关文献
MALARIA JOURNAL文献大全abstract:BACKGROUND:Combination treatments, preferably containing an artemisinin derivative, are recommended to improve efficacy and prevent Plasmodium falciparum drug resistance. Artemether-lumefantrine (AL) and artesunate-amodiaquine (AA) are efficacious regimens that have been widely adopted in sub-Saharan Africa. However, m...
journal_title:Malaria journal
pub_type: 杂志文章,随机对照试验
doi:10.1186/1475-2875-9-335
更新日期:2010-11-22 00:00:00
abstract:BACKGROUND:The pro-inflammatory S100 calcium binding protein A8 (S100A8) is elevated in the serum of patients with Plasmodium falciparum malaria, but its function in Plasmodium vivax malaria is not yet clear. This function was investigated in P. vivax-infected patients in this study. METHODS:The level of S100A8 in the...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0855-4
更新日期:2015-10-05 00:00:00
abstract:BACKGROUND:Many malaria-related studies depend on infected red blood cells (iRBCs) as fundamental material; however, infected blood samples from human or animal models include leukocytes (white blood cells or WBCs), especially difficult to separate from iRBCs in cases involving Plasmodium vivax. These host WBCs are a s...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-10-251
更新日期:2011-08-25 00:00:00
abstract:BACKGROUND:In view of the need to continuously feed the pipeline with new anti-malarial agents adapted to differentiated and more stringent target product profiles (e.g., new modes of action, transmission-blocking activity or long-duration chemo-protection), a chemical library consisting of more than 250,000 compounds ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-13-190
更新日期:2014-05-27 00:00:00
abstract:BACKGROUND:Drug and multidrug-resistant Plasmodium falciparum malaria has existed in Thailand for several decades. Furthermore, Thailand serves as a sentinel for drug-resistant malaria within the Greater Mekong sub-region. However, the drug resistance situation is highly dynamic, changing quickly over time. Here parasi...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-11-290
更新日期:2012-08-21 00:00:00
abstract:BACKGROUND:Behaviour change communication (BCC) can improve malaria prevention and treatment behaviour. As a one of the activities under Myanmar Artemisinin Resistance Containment (MARC) programme, BCC have been conducting. This study aimed to evaluate the effectiveness of the behaviour change communication and communi...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-1047-y
更新日期:2015-12-23 00:00:00
abstract:BACKGROUND:With an Annual Parasite Incidence (API) of 132.1, in the high and moderate risks zones, the Maroni area of French Guiana has the second highest malaria incidence of South-America after Guyana (API = 183.54) and far above Brazil (API = 28.25). Malaria transmission is occurring despite strong medical assistanc...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-80
更新日期:2010-03-22 00:00:00
abstract::The National Vector Borne Disease Control Programme (NVBDCP) of the Ministry of Health, Government of India is reporting about 2 million parasite positive cases each year, although case incidence is 30-fold or more under-estimated. Forty five to fifty percent of Plasmodium infections are caused by Plasmodium falciparu...
journal_title:Malaria journal
pub_type: 社论
doi:10.1186/1475-2875-6-105
更新日期:2007-08-07 00:00:00
abstract:BACKGROUND:Antiplasmodial activities of angiotensin II and its analogues have been extensively investigated in Plasmodium gallinaceum and Plasmodium falciparum parasite species. Due to its vasoconstrictor property angiotensin II cannot be used as an anti-malarial drug. METHODS:This work presents the solid-phase synthe...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-015-0974-y
更新日期:2015-11-04 00:00:00
abstract:BACKGROUND:Intermittent preventive (or presumptive) treatment of infants (IPTi), the administration of a curative anti-malarial dose to infants whether or not they are known to be infected, is being considered as a new strategy for malaria control. Five of the six trials using sulphadoxine-pyrimethamine (SP) for IPTi s...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-7-54
更新日期:2008-04-03 00:00:00
abstract::The hypnozoite reservoir of Plasmodium vivax represents both the greatest obstacle and opportunity for ultimately eradicating this species. It is silent and cannot be diagnosed until it awakens and provokes a clinical attack with attendant morbidity, risk of mortality, and opportunities for onward transmission. The on...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2190-z
更新日期:2018-01-22 00:00:00
abstract:BACKGROUND:Declining in clinical efficacy of artesunate-mefloquine combination has been documented in areas along the eastern border (Thai-Cambodian) of Thailand. In the present study, the clinical efficacy of the three-day combination regimen of artesunate-mefloquine as first-line treatment for acute uncomplicated fal...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-273
更新日期:2010-10-08 00:00:00
abstract:BACKGROUND:Intermittent preventive treatment with sulphadoxine-pyrimethamine (SP) is recommended for reducing the risk of malaria in pregnancy and its consequences on mothers and babies (IPTp-SP). Indicators of implementation and effects of IPTp-SP were collected in a rural clinic in Southern Senegal. METHODS:Women se...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-7-234
更新日期:2008-11-07 00:00:00
abstract:BACKGROUND:Rapid diagnostic tests (RDTs) have become the most common diagnostic tool for detection of Plasmodium falciparum malaria, in particular in remote areas. RDT blood spots provide a source of parasite DNA for molecular analysis. In this study, the utility of RDTs for molecular analysis and the performance of di...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-03259-9
更新日期:2020-05-27 00:00:00
abstract:BACKGROUND:Asymptomatic carriage of Plasmodium falciparum is widespread in adults and children living in malaria-endemic countries. This study identified the prevalence of malaria parasites and the corresponding levels of naturally acquired anti-parasite antibody levels in afebrile adults living in two communities in t...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-3146-7
更新日期:2020-02-10 00:00:00
abstract:BACKGROUND:The use of insecticide-treated nets (ITN) is an important tool in the Roll Back Malaria (RBM) strategy. For ITNs to be effective they need to be used correctly. Previous studies have shown that many factors, such as wealth, access to health care, education, ethnicity and gender, determine the ownership and u...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-175
更新日期:2009-07-29 00:00:00
abstract:BACKGROUND:Effective and timely treatment is an essential aspect of malaria control, but remains a challenge in many parts of sub-Saharan Africa. The objective of this study was to describe young children's access to malaria treatment in Nouna Health District, Burkina Faso. METHODS:In February/March 2006, a survey was...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-8-266
更新日期:2009-11-24 00:00:00
abstract:BACKGROUND:Anti-malarial drugs are an important tool for malaria control and elimination. Alongside their direct benefit in the treatment of disease, drug use has a community-level effect, clearing the reservoir of infection and reducing onward transmission of the parasite. Different compounds potentially have differen...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-017-1988-4
更新日期:2017-08-16 00:00:00
abstract:BACKGROUND:Marking mosquitoes is vital for mark-release-recapture and many laboratory studies, but their small size precludes the use of methods that are available for larger animals such as unique identifier tags and radio devices. Fluorescent dust is the most commonly used method to distinguish released individuals f...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-020-03306-5
更新日期:2020-07-06 00:00:00
abstract:BACKGROUND:The effectiveness of vector control on malaria transmission by long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) depends on the vectors entering houses to blood feed and rest when people are inside houses. In the Solomon Islands, significant reductions in malaria have been achieved in...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1195-8
更新日期:2016-03-10 00:00:00
abstract:BACKGROUND:Several anti-malarial drugs are associated with adverse cardiovascular effects. These effects may be exacerbated when different anti-malarials are used in combination. There has been no report yet on the potential cardiac effects of the combination artesunate-amodiaquine. METHODS:Electrocardiographic (ECG) ...
journal_title:Malaria journal
pub_type: 临床试验,杂志文章
doi:10.1186/1475-2875-11-420
更新日期:2012-12-17 00:00:00
abstract:BACKGROUND:The relationship between the burden of uncomplicated malaria and transmission intensity is unclear and a better understanding of this relationship is important for the implementation of intervention programmes. METHODS:A 6-month longitudinal study monitoring risk factors for anaemia and febrile malaria epis...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-3-26
更新日期:2004-07-28 00:00:00
abstract:BACKGROUND:Knowledge of the ecological differences between the molecular forms of Anopheles gambiae and their sibling species, An. arabiensis might lead to understanding their unique contribution to disease transmission and to better vector control as well as to understanding the evolutionary forces that have separated...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-5-19
更新日期:2006-03-22 00:00:00
abstract:BACKGROUND:Indoor-based anti-vector interventions remain the preferred means of reducing risk of malaria transmission in malaria endemic areas around the world. Despite demonstrated success in reducing human-mosquito interactions, these methods are effective solely against endophilic vectors. It may be that outdoor loc...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-10-184
更新日期:2011-07-07 00:00:00
abstract:BACKGROUND:The merozoite surface protein 1 (msp1) is one of the most studied vaccine candidate genes in mammalian Plasmodium spp. to have been used for investigations of epidemiology, population structures, and immunity to infections. However methodological difficulties have impeded the use of nuclear markers such as m...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-12-381
更新日期:2013-10-30 00:00:00
abstract:BACKGROUND:Plasmodium falciparum circumsporozoite protein (PfCSP) is one of the most extensively studied malaria vaccine candidates, but the genetic polymorphism of PfCSP within and among the global P. falciparum population raises concerns regarding the efficacy of a PfCSP-based vaccine efficacy. In this study, genetic...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-018-2513-0
更新日期:2018-10-12 00:00:00
abstract:BACKGROUND:Individuals in a malaria endemic community differ from one another. Many of these differences, such as heterogeneities in transmission or treatment-seeking behaviour, affect malaria epidemiology. The different kinds of heterogeneity are likely to be correlated. Little is known about their impact on the shape...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/1475-2875-9-132
更新日期:2010-05-17 00:00:00
abstract::Natural compounds, mostly from plants, have been the mainstay of traditional medicine for thousands of years. They have also been the source of lead compounds for modern medicine, but the extent of mining of natural compounds for such leads decreased during the second half of the 20th century. The advantage of natural...
journal_title:Malaria journal
pub_type: 杂志文章,评审
doi:10.1186/1475-2875-10-S1-S1
更新日期:2011-03-15 00:00:00
abstract:BACKGROUND:Slash and burn cultivators are a significant risk group for malaria in South-East Asia. As envisaged in the National Strategic Plan for Malaria Elimination, Bangladesh aims to achieve zero indigenous malaria transmission by 2030. For the national plan to move from malaria control to malaria elimination, targ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-019-2849-0
更新日期:2019-06-25 00:00:00
abstract:BACKGROUND:Within the context of combined interventions, malaria vaccine may provide additional value in malaria prevention. Stakeholders' perspectives are thus critical for informed recommendation of the vaccine in Tanzania. This paper presents the views of stakeholders with regards to malaria vaccine in 12 Tanzanian ...
journal_title:Malaria journal
pub_type: 杂志文章
doi:10.1186/s12936-016-1209-6
更新日期:2016-04-05 00:00:00