Immune response evoked by tumor-associated NADH oxidase (tNOX) confers potential inhibitory effect on lung carcinoma in a mouse model.

Abstract:

:Tumor-associated NADH oxidase (tNOX, ENOX2), which belongs to a family of growth-related NADH oxidases, was originally identified as a plasma membrane protein of rat hepatoma and is inhibited or downregulated by several anti-cancer drugs. The objective of this study was to evaluate the anti-tumor effects of tNOX used as an immunogen against Lewis lung cancer. Human tNOX was expressed in Escherichia coli, purified by His-Tag affinity chromatography, and emulsified with the adjuvant, ISA 201 VG. Immunological analyses of the generated tNOX vaccine were performed in mice. The results of ELISA and ELISpot were significantly higher in tNOX vaccine group compared to the control group. In vivo, we examined the anti-tumor effects of mice that received the tNOX vaccine via the intraperitoneal or subcutaneous routes. Mice were vaccinated three times at 2-week intervals, challenged at 2 weeks after the final vaccination, and terminated at 34 days post-challenge. Antibody titers, tumor volume and histopathological scores were used to evaluate the anti-tumor effects of the tNOX vaccine. Our results revealed that tNOX-vaccinated mice had significantly higher antibody titers than negative control (NC) and challenge control (CC) mice. When compared to the corresponding CC groups, the intraperitoneal and subcutaneous vaccination with tNOX showed a significantly smaller tumor mass volume (P < 0.05) and a significantly lower histological lesion score (P < 0.05), respectively. Our results demonstrate that the use of a xenogeneic tNOX as an immunogen in mice activates immune responses and anti-tumor effects against Lewis lung cancer.

journal_name

Am J Cancer Res

authors

Hsieh PF,Chueh PJ,Liu PF,Liao JW,Hsieh MK

subject

Has Abstract

pub_date

2019-04-01 00:00:00

pages

740-751

issue

4

issn

2156-6976

journal_volume

9

pub_type

杂志文章
  • Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion.

    abstract::The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: de Abreu AL,Malaguti N,Souza RP,Uchimura NS,Ferreira ÉC,Pereira MW,Carvalho MD,Pelloso SM,Bonini MG,Gimenes F,Consolaro ME

    更新日期:2016-06-01 00:00:00

  • Interferon regulatory factor-1 suppresses DNA damage response and reverses chemotherapy resistance by downregulating the expression of RAD51 in gastric cancer.

    abstract::Recent studies have shown that IRF-1 plays a significant role in various tumour-induced chemoresistance, but its role and mechanism in gastric cancer-associated chemoresistance are not clear. Our study showed that IRF-1 expression could reverse gastric cancer-related chemoresistance. Dysregulated DNA repair is an impo...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Tan L,Yuan J,Zhu W,Tao K,Wang G,Gao J

    更新日期:2020-04-01 00:00:00

  • Oncolytic newcastle disease virus triggers cell death of lung cancer spheroids and is enhanced by pharmacological inhibition of autophagy.

    abstract::Lung cancer stem cells (CSCs) have recently been isolated from lung cancer patient samples and have been reported to be responsible for tumor initiation, treatment resistance and tumor recurrence. We have previously shown that oncolytic Newcastle disease virus (NDV), strain FMW (NDV/FMW) induces apoptosis in drug-resi...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Hu L,Sun S,Wang T,Li Y,Jiang K,Lin G,Ma Y,Barr MP,Song F,Zhang G,Meng S

    更新日期:2015-11-15 00:00:00

  • Monocyte recruitment and activated inflammation are associated with thyroid carcinogenesis in a mouse model.

    abstract::Thyroid cancer is the most common endocrine malignancy. Although an association between inflammation and thyroid cancer has long been recognized, a cause-effect relationship at the molecular level has yet to be elucidated. We explored how inflammation could contribute to thyroid carcinogenesis in ThrbPV/PVPten+/- mice...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Park S,Zhu J,Altan-Bonnet G,Cheng SY

    更新日期:2019-07-01 00:00:00

  • Chiauranib selectively inhibits colorectal cancer with KRAS wild-type by modulation of ROS through activating the p53 signaling pathway.

    abstract::Colorectal cancer (CRC) is one of the top three most deadly cancers despite using chemotherapy based on oxaliplatin or irinotecan combined with targeted therapy. Chiauranib has recently been identified to be a promising anticancer candidate with impressive efficacy and safety. However, the role and molecular mechanism...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Yin H,Xie J,Jiang P,Jiang X,Duan D,Qi J,Luo Z,Ma C,Hong H

    更新日期:2020-11-01 00:00:00

  • Does the expression of BCL2 have prognostic significance in malignant peritoneal mesothelioma?

    abstract:UNLABELLED:Background Malignant peritoneal mesothelioma (MPM) is a rare neoplasm of the peritoneal membrane that is causally related to asbestos exposure. Survival after treatment is poor. Current therapy involving hyperthermic intraperitoneal chemotherapy has improved survival in selective patients. In the past, sever...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Pillai K,Pourgholami MH,Chua TC,Morris DL

    更新日期:2013-06-20 00:00:00

  • Genetic variants of BCL2 gene predict clinical outcomes of non-small-cell lung cancer patients treated with platinum-based chemotherapy in a Chinese population.

    abstract::Platinum agents induce cancer cell death through BCL2-dependent intrinsic apoptotic pathway and are commonly used as anti-tumor drug. In this study, we evaluated whether single nucleotide polymorphism (SNPs) of BCL2 can affect the overall survival (OS) and progression-free survival (PFS) in non-small-cell lung cancer ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Ding X,Qian J,Yang Y,Xu W,Liu D,Su B

    更新日期:2016-10-01 00:00:00

  • Mismatch repair-based stratification for immune checkpoint blockade therapy.

    abstract::Mismatch repair (MMR) plays a key role in maintaining genomic stability. Mismatch repair deficiency (MMR-D) causes a molecular feature of microsatellite instability (MSI) and contributes to the development of human cancers and genetic diseases with cancer predisposition such as Lynch syndrome. Recent studies have show...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Zhang L,Peng Y,Peng G

    更新日期:2018-10-01 00:00:00

  • High levels of KLK7 protein expression are related to a favorable prognosis in triple-negative breast cancer patients.

    abstract::In normal physiology, kallikrein-related peptidase 7 (KLK7), together with other members of the kallikrein-related peptidase family, is mainly involved in skin desquamation and keratinization processes. Moreover, expression of KLK7 was shown in various tumor types to be dysregulated and to correlate to patients' survi...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Geng X,Babayeva L,Walch A,Aubele M,Groß E,Kiechle M,Bronger H,Dreyer T,Magdolen V,Dorn J

    更新日期:2020-06-01 00:00:00

  • Downregulation of ACSM3 promotes metastasis and predicts poor prognosis in hepatocellular carcinoma.

    abstract::Understanding mechanisms of cancer metastasis is crucial for reduction of cancer mortality. Acyl-CoA medium-chain synthetase 3 (ACSM3) is an acyl-CoA synthetase which takes part in the first step of fatty acid metabolism. However, the expression, clinical significance and biological function of ACSM3 remain unknown in...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Ruan HY,Yang C,Tao XM,He J,Wang T,Wang H,Wang C,Jin GZ,Jin HJ,Qin WX

    更新日期:2017-03-01 00:00:00

  • Long noncoding RNA CASC11 promotes osteosarcoma metastasis by suppressing degradation of snail mRNA.

    abstract::Osteosarcoma (OS) is the most common primary bone tumor in adolescents. Dysregulation of long noncoding RNAs (lncRNAs) is associated with the cancer progression, of which cancer susceptibility candidate 11 (CASC11) has been indicated as an oncogene in several human cancers. However, the underlying mechanisms by which ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,收录出版

    doi:

    authors: Song K,Yuan X,Li G,Ma M,Sun J

    更新日期:2019-02-01 00:00:00

  • Everolimus following 5-aza-2-deoxycytidine is a promising therapy in paclitaxel-resistant clear cell carcinoma of the ovary.

    abstract::Our previous study showed that 5-aza-2-deoxycytidine (5-aza-dC) could inhibit tumor growth by enhancing the susceptibility of ovarian clear cell carcinoma (OCCC) to paclitaxel through decreasing AKT/mTOR expressions. The objective of the study is to evaluate the antitumor efficacy of everolimus (RAD001) and 5-aza-2-de...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Ho CM,Lee FK,Huang SH,Cheng WF

    更新日期:2018-01-01 00:00:00

  • Nanoparticle-mediated down-regulation of TWIST increases radiosensitivity of nasopharyngeal carcinoma cells via ERK pathway.

    abstract::Evidence suggests that over-expression of TWIST, an epithelial-mesenchymal transition inducer, might have a correlation with cancer progression and chemoresistance. However, its roles in radioresistance of cancer have rarely been reported. High TWIST expression was detected in nasopharyngeal carcinoma (NPC) and associ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Zhuo X,Chang A,Huang C,Yang L,Zhao H,Wu Y,Zhou Q

    更新日期:2015-03-15 00:00:00

  • NQO1 is a determinant for cellular sensitivity to anti-tumor agent Napabucasin.

    abstract::Napabucasin (NAPA) is thought to be a potent cancer stemness inhibitor in different types of cancer cell lines. While it has shown promising activity in early phase clinical trials, two recent phase III NAPA clinical trials failed to meet the primary endpoint of overall survival. The reason for the failure is not clea...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Guo G,Gao Z,Tong M,Zhan D,Wang G,Wang Y,Qin J

    更新日期:2020-05-01 00:00:00

  • Verteporfin inhibits YAP function through up-regulating 14-3-3σ sequestering YAP in the cytoplasm.

    abstract::Yes-associated protein (YAP), the central mediator of Hippo pathway, not only regulates a diversity of cellular processes during development but also plays a pivotal role in tumorigenesis. YAP is overexpressed in many types of human cancers with its expression level being associated with patient outcomes. Thus, inhibi...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Wang C,Zhu X,Feng W,Yu Y,Jeong K,Guo W,Lu Y,Mills GB

    更新日期:2015-12-15 00:00:00

  • SLFN5 influences proliferation and apoptosis by upregulating PTEN transcription via ZEB1 and inhibits the purine metabolic pathway in breast cancer.

    abstract::Human Schlafen-5 (SLFN5) is aberrantly involved in tumorigenesis in several types of cancer. However, its implications in breast cancer (BRCA) are unknown. Herein, we demonstrated that SLFN5 expression is negatively associated with the tumour growth of human BRCA using GEO database analysis and clinical sample immunos...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Gu X,Wan G,Yang Y,Liu Y,Yang X,Zheng Y,Jiang L,Zhang P,Liu D,Zhao W,Huang G,Lu C

    更新日期:2020-09-01 00:00:00

  • HOXD-AS1 promotes cell proliferation, migration and invasion through miR-608/FZD4 axis in ovarian cancer.

    abstract::Evidence is accumulating that long non-coding RNAs (lncRNAs) exert crucial roles in the incidence and progression of tumors. HOXD cluster antisense RNA 1 (HOXD-AS1), a cancer-related lncRNA, has been frequently reported to be involved in tumorigenesis and dysregulated in multiple types of human cancers; however, littl...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Wang Y,Zhang W,Wang Y,Wang S

    更新日期:2018-01-01 00:00:00

  • Stromal modulation of bladder cancer-initiating cells in a subcutaneous tumor model.

    abstract::The development of new cancer therapeutics would benefit from incorporating efficient tumor models that mimic human disease. We have developed a subcutaneous bladder tumor regeneration system that recapitulates primary human bladder tumor architecture by recombining benign human fetal bladder stromal cells with SW780 ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Peek EM,Li DR,Zhang H,Kim HP,Zhang B,Garraway IP,Chin AI

    更新日期:2012-01-01 00:00:00

  • TrkC expression predicts favorable clinical outcome in invasive ductal carcinoma of breast independent of NT-3 expression.

    abstract:BACKGROUND:TrkC, a member of neurotrophin receptor family, functions not only as an oncogene, but also act as a tumor suppressor via a manner of dependence receptor in human malignant tumors. Little is known on the action of TrkC for the clinical prognosis and the progression of breast cancer according to the availabil...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Zhang W,Lin ZC,Zhang TX,Liu S,Liu X,Liu JJ,Niu Y

    更新日期:2014-11-19 00:00:00

  • Overexpression of MEF2D contributes to oncogenic malignancy and chemotherapeutic resistance in ovarian carcinoma.

    abstract::The transcription factor MEF2 promotes survival in various cell types and a number of studies indicate that abnormal regulation of MEF2 is linked to oncogenicity in several carcinomas. We have found that MEF2D, a member of the MEF2 family, is upregulated in Ovarian Cancer (OC). Immunohistochemistry analysis of tumor s...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Li X,Zhang Y,Chai X,Zhou S,Zhang H,He J,Zhou R,Cai L,Chen L,Tao G

    更新日期:2019-05-01 00:00:00

  • [6]-Gingerol inhibits de novo fatty acid synthesis and carnitine palmitoyltransferase-1 activity which triggers apoptosis in HepG2.

    abstract::The de novo fatty acid synthesis catalyzed by key lipogenic enzymes, including fatty acid synthase (FASN) has emerged as one of the novel targets of anti-cancer approaches. The present study explored the possible inhibitory efficacy of [6]-gingerol on de novo fatty acid synthesis associated with mitochondrial-dependen...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Impheng H,Richert L,Pekthong D,Scholfield CN,Pongcharoen S,Pungpetchara I,Srisawang P

    更新日期:2015-03-15 00:00:00

  • Identification of HRAS as cancer-promoting gene in gastric carcinoma cell aggressiveness.

    abstract::Gastric carcinoma is one of the most lethal malignancies of cancers and its prognosis remains dismal due to the paucity of effective therapeutic targets. Herein, we showed that HRAS is markedly up-regulated in gastric carcinoma. Prognostic analysis indicated that HRAS expression might be a prognostic indicator for the...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Wu XY,Liu WT,Wu ZF,Chen C,Liu JY,Wu GN,Yao XQ,Liu FK,Li G

    更新日期:2016-09-01 00:00:00

  • Clinical characteristics and responses to chemotherapy and immune checkpoint inhibitor treatment for microsatellite instability gastric cancer.

    abstract::During the process of DNA replication, insertions or deletions of repeat sequences easily occur in microsatellites due to DNA polymerase slippage in instances of defective mismatch repair; this phenomenon is known as microsatellite instability. Based on genetic profiling, microsatellite instability gastric cancer is r...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Yang G,Zheng RY,Tan Q,Dong CJ,Jin ZS

    更新日期:2020-12-01 00:00:00

  • Hispidulin suppresses tumor growth and metastasis in renal cell carcinoma by modulating ceramide-sphingosine 1-phosphate rheostat.

    abstract::Sphingosine 1-phosphate (S1P) rheostat is considered as a key signal that determines cell fate. This study aimed to report that hispidulin, a polyphenolic flavonoid, exerted anti-growth and anti-metastasis effects against renal cell carcinoma (RCC) by modulating the balance of ceramide-S1P. In vitro studies showed tha...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,收录出版

    doi:

    authors: Gao MQ,Gao H,Han M,Liu KL,Peng JJ,Han YT

    更新日期:2017-07-01 00:00:00

  • Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence.

    abstract::Gamma-glutamylcyclotransferase (GGCT) was originally identified as a protein highly expressed in bladder cancer tissues by proteomic analysis, and its higher expression in a variety of cancers compared to normal tissues have been shown. Depletion of GGCT in various cancer cells results in antiproliferative effects bot...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Taniguchi K,Matsumura K,Ii H,Kageyama S,Ashihara E,Chano T,Kawauchi A,Yoshiki T,Nakata S

    更新日期:2018-04-01 00:00:00

  • Neural correlates of chemotherapy-induced emotion regulation impairment in breast cancer patients.

    abstract::To investigate the chemotherapy-induced emotion regulation impairment and its neural correlates in breast cancer (BC) patients by event-related potentials (ERP), seventeen BC patients were investigated on emotion regulation paradigms while undergoing the recording of an event-related potential (ERP) both before and af...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Gan C,Lv Y,Zhao J,Chao HH,Li CR,Zhang C,Yu F,Cheng H

    更新日期:2019-01-01 00:00:00

  • Advances in malignant pleural mesothelioma therapy: targeting EphA2 a novel approach.

    abstract::Malignant pleural mesothelioma (MPM) is an aggressive neoplasm with a poor prognosis. MPM grows from the mesothelial cells lining the surface of the lung and chest wall called Pleura. Exposure to asbestos is mainly linked to the development of MPM. Approximately 80% of the tumors are pleural in origin, and up to 3000 ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Nasreen N,Khodayari N,Mohammed KA

    更新日期:2012-01-01 00:00:00

  • Serum glycoprotein-derived N- and O-linked glycans as cancer biomarkers.

    abstract::Early detection of cancer is the key to improving survival. Since most clinically used serum cancer biomarkers are either glycoproteins or glycan structures that can be recognized by specific monoclonal antibodies, developing glycan structure-based biomarkers from human serum/plasma glycoproteins through mass spectrom...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Lan Y,Hao C,Zeng X,He Y,Zeng P,Guo Z,Zhang L

    更新日期:2016-11-01 00:00:00

  • TLR4 activation leads to anti-EGFR therapy resistance in head and neck squamous cell carcinoma.

    abstract::Epidermal growth factor receptor (EGFR) is highly expressed in head and neck squamous cell carcinoma (HNSCC) and related to cancer progression. The resistance to anti-EGFR therapy remains a major clinical problem in HNSCC. In this study, we found that TOLL-like receptor 4 (TLR4) was highly expressed in 50% of EGFR ove...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Ju H,Hu Z,Lu Y,Wu Y,Zhang L,Wei D,Guo W,Xia W,Liu S,Ren G,Hu J

    更新日期:2020-02-01 00:00:00

  • Chemopreventive and anticancer activity of flavonoids and its possibility for clinical use by combining with conventional chemotherapeutic agents.

    abstract::Cancer is a diverse class of diseases characterized by uncontrolled cell growth with the potential to invade and spread to other parts of the body, and continues to be one of the leading causes of death worldwide. Conventional cancer treatment modalities include antitumor drugs, surgical resection, locally targeted th...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Kikuchi H,Yuan B,Hu X,Okazaki M

    更新日期:2019-08-01 00:00:00