Abstract：
:Our previous study showed that 5-aza-2-deoxycytidine (5-aza-dC) could inhibit tumor growth by enhancing the susceptibility of ovarian clear cell carcinoma (OCCC) to paclitaxel through decreasing AKT/mTOR expressions. The objective of the study is to evaluate the antitumor efficacy of everolimus (RAD001) and 5-aza-2-deoxycytidine (5-aza-dC) by targeting AKT/mTOR and EZH2 in OCCC. Paclitaxel-sensitive and resistant OCCC cell lines were established. In vitro proliferative and apoptotic assays and flow cytometry were performed. The expressions of EZH2, PIK3IP1, phospho-AKT, phospho-mTOR and phospho-Rictor in the OCCC cell lines were evaluated by Western blotting. In vivo animal experiments with RAD001 and 5-aza-dC were performed. RAD001 alone showed significant in vitro antitumor activity and inhibited in vivo tumor growth in paclitaxel-sensitive and resistant OCCC cells. In addition, 5-aza-dC enhanced the antitumor effects when combined with paclitaxel or RAD001 in both paclitaxel-sensitive and resistant tumors. Activation of phospho-AKT ser473 and PIK3IP1 was observed in RAD001-treated paclitaxel-sensitive and resistant OCCC cells. In contrast, inhibition of phospho-AKT ser473 and EZH2 was observed with RAD001 following 5-aza-dC treatment of paclitaxel-sensitive and resistant OCCC cells. Furthermore, RAD001 following 5-aza-dC enhanced apoptosis of paclitaxel-sensitive and resistant OCCC cells. RAD001 following 5-aza-dC may be a promising treatment strategy for the treatment of both chemo-sensitive and resistant OCCC. Further clinical studies are warranted.
journal_name
Am J Cancer Resjournal_title
American journal of cancer researchauthors
Ho CM,Lee FK,Huang SH,Cheng WFsubject
Has Abstractpub_date
2018-01-01 00:00:00pages
56-69issue
1issn
2156-6976journal_volume
8pub_type
杂志文章abstract：:RABEX-5 has been studied in various solid tumors, but its role in non-small-cell lung cancer (NSCLC) remains unknown. This study is aimed to investigate the expression, the potential relevance to clinicopathological characters and prognostic significance of RABEX-5 in patients with NSCLC. A total of 120 NSCLC patients...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2015-08-15 00:00:00
abstract：:Factors within the tissue of breast cancer (BC) may shift the polarization of CD4+ T cells towards Th2 direction. This tendency can promote tumor development and be enhanced by the use of tamoxifen during the treatment. Thus, the patients with low levels of tumor-induced Th2 polarization prior to tamoxifen treatment m...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2015-01-15 00:00:00
abstract：:Colorectal cancer is one of the most commonly diagnosed malignancies among males and females worldwide. Although China is a country with a low incidence of colorectal cancer, with the improvement of China's economy and lifestyle changes, the incidence rate in China has generally increased in recent years, and the morb...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2019-11-01 00:00:00
abstract：BACKGROUND:Gliomas are the most common aggressive brain tumors and have many complex pathological types. Previous reports have discovered that genetic mutations are associated with the risk of glioma. However, it is unclear whether uniform genetic mutations exist difference between glioma and its two pathological types...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2015-06-15 00:00:00
abstract：:MLN4924 is an experimental cancer drug known as inhibitor of NEDD8-activating enzyme (NAE). This anti-tumor candidate is a selective small-molecule inhibitor of NAE which is conjugated to cullin protein on Cullin-RING ligases (CRLs). This covalent modification actives cullin complex to recruit an ubiquitin-charged E2 ...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2015-10-15 00:00:00
abstract：:Increasing evidence reveals that deregulation of miRNAs contributes to carcinogenesis of the human non-small cell lung cancer (NSCLC). Our study discovered that the expression of miR-449a was markedly decreased in NSCLC cells with high metastatic capacity and tissues of positive lymph node metastasis. Moreover, our re...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2015-08-15 00:00:00
abstract：:A polymorphic mutation in the acetaldehyde dehydrogenase 2 (ALDH2) gene has been epidemiologically linked to the high susceptibility to esophageal carcinogenesis for individuals with alcohol use disorders. Mice subjected to alcohol drinking show increased oxidative stress and DNA adduct formation in esophageal epithel...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2016-03-15 00:00:00
abstract：:Early stages of melanoma can be successfully treated by surgical resection of the tumor, but there is still no effective treatment once it is progressed to metastatic phases. Although growing family of both melanoma metastasis promoting and metastasis suppressor genes have been reported be related to metastasis, the m...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2015-08-15 00:00:00
abstract：:Osteosarcoma (OS) is the most common primary bone tumor in adolescents. Dysregulation of long noncoding RNAs (lncRNAs) is associated with the cancer progression, of which cancer susceptibility candidate 11 (CASC11) has been indicated as an oncogene in several human cancers. However, the underlying mechanisms by which ...
journal_title：American journal of cancer research
pub_type： 杂志文章,收录出版
doi：
更新日期：2019-02-01 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章,已发布勘误
doi：
更新日期：2020-06-01 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2017-12-01 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) remains to be a challenging public health problem worldwide. However, the underlying molecular mechanism regulating the carcinogenesis of OSCC is poorly known. Gene expression profiles of GSE13601, GSE30784, GSE37991 and The Cancer Genome Atlas (TCGA) head and neck cancer were downl...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2018-03-01 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章,评审
doi：
更新日期：2020-06-01 00:00:00
abstract：:Glioma is one of the most common, rapidly progressive and fatal brain tumors, and accumulating evidence shows that microRNAs (miRNAs) play important roles in the development of cancers, including glioma. Therapeutic applications of miRNAs in Ras-driven glioma have been proposed; however, their specific functions and m...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
abstract：:The transcription factor MEF2 promotes survival in various cell types and a number of studies indicate that abnormal regulation of MEF2 is linked to oncogenicity in several carcinomas. We have found that MEF2D, a member of the MEF2 family, is upregulated in Ovarian Cancer (OC). Immunohistochemistry analysis of tumor s...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2019-05-01 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章,收录出版
doi：
更新日期：2017-07-01 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2016-01-15 00:00:00
abstract：:The development of new cancer therapeutics would benefit from incorporating efficient tumor models that mimic human disease. We have developed a subcutaneous bladder tumor regeneration system that recapitulates primary human bladder tumor architecture by recombining benign human fetal bladder stromal cells with SW780 ...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Gamma-glutamylcyclotransferase (GGCT) was originally identified as a protein highly expressed in bladder cancer tissues by proteomic analysis, and its higher expression in a variety of cancers compared to normal tissues have been shown. Depletion of GGCT in various cancer cells results in antiproliferative effects bot...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2018-04-01 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2020-12-01 00:00:00
abstract：:Colorectal cancer (CRC) is one of the top three most deadly cancers despite using chemotherapy based on oxaliplatin or irinotecan combined with targeted therapy. Chiauranib has recently been identified to be a promising anticancer candidate with impressive efficacy and safety. However, the role and molecular mechanism...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2020-11-01 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章,评审
doi：
更新日期：2015-04-15 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2013-06-20 00:00:00
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journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2014-01-15 00:00:00
abstract：:Human Schlafen-5 (SLFN5) is aberrantly involved in tumorigenesis in several types of cancer. However, its implications in breast cancer (BRCA) are unknown. Herein, we demonstrated that SLFN5 expression is negatively associated with the tumour growth of human BRCA using GEO database analysis and clinical sample immunos...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2020-09-01 00:00:00
abstract：:Afatinib is the second generation of irreversible inhibitor of EGFR, HER2 and HER4, which has shown encouraging phase II and III clinical outcomes in the treatment of head and neck squamous cell carcinoma (HNSCC). However, the molecular mechanism of afatinib-induced apoptosis in HNSCC is poorly understood. In the pres...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：:SOX2 is an embryonic stem cell marker that in prostate cancer has been associated not only with tumorigenesis but also metastasis. Furthermore hypoxia in primary tumors has been linked to poor prognosis and outcomes in this disease. The goal of the present study was to investigate the impact of hypoxia on SOX2 express...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：:Pancreatic cancer (PC) is one of the most common gastrointestinal malignancies that are highly aggressive with a low 5-year survival rate. Accumulated evidence has indicated that decoy receptor 3 (DcR3) is involved in several pathologic processes and various cancers. However, the mechanisms underlying dysregulated DcR...
journal_title：American journal of cancer research
pub_type： 杂志文章
doi：
更新日期：2019-12-01 00:00:00
