Everolimus following 5-aza-2-deoxycytidine is a promising therapy in paclitaxel-resistant clear cell carcinoma of the ovary.

Abstract:

:Our previous study showed that 5-aza-2-deoxycytidine (5-aza-dC) could inhibit tumor growth by enhancing the susceptibility of ovarian clear cell carcinoma (OCCC) to paclitaxel through decreasing AKT/mTOR expressions. The objective of the study is to evaluate the antitumor efficacy of everolimus (RAD001) and 5-aza-2-deoxycytidine (5-aza-dC) by targeting AKT/mTOR and EZH2 in OCCC. Paclitaxel-sensitive and resistant OCCC cell lines were established. In vitro proliferative and apoptotic assays and flow cytometry were performed. The expressions of EZH2, PIK3IP1, phospho-AKT, phospho-mTOR and phospho-Rictor in the OCCC cell lines were evaluated by Western blotting. In vivo animal experiments with RAD001 and 5-aza-dC were performed. RAD001 alone showed significant in vitro antitumor activity and inhibited in vivo tumor growth in paclitaxel-sensitive and resistant OCCC cells. In addition, 5-aza-dC enhanced the antitumor effects when combined with paclitaxel or RAD001 in both paclitaxel-sensitive and resistant tumors. Activation of phospho-AKT ser473 and PIK3IP1 was observed in RAD001-treated paclitaxel-sensitive and resistant OCCC cells. In contrast, inhibition of phospho-AKT ser473 and EZH2 was observed with RAD001 following 5-aza-dC treatment of paclitaxel-sensitive and resistant OCCC cells. Furthermore, RAD001 following 5-aza-dC enhanced apoptosis of paclitaxel-sensitive and resistant OCCC cells. RAD001 following 5-aza-dC may be a promising treatment strategy for the treatment of both chemo-sensitive and resistant OCCC. Further clinical studies are warranted.

journal_name

Am J Cancer Res

authors

Ho CM,Lee FK,Huang SH,Cheng WF

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

56-69

issue

1

issn

2156-6976

journal_volume

8

pub_type

杂志文章
  • Polycomb protein family member CBX7 plays a critical role in cancer progression.

    abstract::CBX7 is a polycomb protein that participates in the formation of polycomb repressive complex 1. Apart from few exceptions, CBX7 expression is lost in human malignant neoplasias and a clear correlation between its downregulated expression and a cancer aggressiveness and poor prognosis has been observed. These findings ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Pallante P,Forzati F,Federico A,Arra C,Fusco A

    更新日期:2015-04-15 00:00:00

  • Clinical characteristics and responses to chemotherapy and immune checkpoint inhibitor treatment for microsatellite instability gastric cancer.

    abstract::During the process of DNA replication, insertions or deletions of repeat sequences easily occur in microsatellites due to DNA polymerase slippage in instances of defective mismatch repair; this phenomenon is known as microsatellite instability. Based on genetic profiling, microsatellite instability gastric cancer is r...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Yang G,Zheng RY,Tan Q,Dong CJ,Jin ZS

    更新日期:2020-12-01 00:00:00

  • Control of tissue size and development by a regulatory element in the yorkie 3'UTR.

    abstract::Regulation of the Hippo pathway via phosphorylation of Yorkie (Yki), the Drosophila homolog of human Yes-associated protein 1, is conserved from Drosophila to humans. Overexpression of a non-phosphorylatable form of Yki induces severe overgrowth in adult fly eyes. Here, we show that yki mRNA associates with microsomal...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Umegawachi T,Yoshida H,Koshida H,Yamada M,Ohkawa Y,Sato T,Suyama M,Krause HM,Yamaguchi M

    更新日期:2017-03-01 00:00:00

  • Histone modifications and cancer: biomarkers of prognosis?

    abstract::Epigenetic dysregulation is being increasingly recognized as a hallmark of cancer. Post-translational modifications of histones, in particular, are known to play important roles gene expression alterations in cancer development and progression. Given their key involvement in the various stages of carcinogenesis, histo...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Chervona Y,Costa M

    更新日期:2012-01-01 00:00:00

  • GPX3 hypermethylation serves as an independent prognostic biomarker in non-M3 acute myeloid leukemia [Retraction].

    abstract::[This retracts the article on p. 2047 in vol. 5, PMID: 26269763.]. ...

    journal_title:American journal of cancer research

    pub_type: 撤回出版物

    doi:

    authors:

    更新日期:2016-10-01 00:00:00

  • Low expression of RBMS3 and SFRP1 are associated with poor prognosis in patients with gastric cancer.

    abstract::RNA binding motif, single stranded interacting protein 3 (RBMS3) has been reported as a tumor suppressor gene (TSG) in some squamous carcinoma. However, its expression levels and clinical significance in gastric cancer (GC) remains unclear. Secreted frizzled-related protein 1 (SFRP1) plays a role of tumor suppressor i...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Zhang T,Wu Y,Fang Z,Yan Q,Zhang S,Sun R,Khaliq J,Li Y

    更新日期:2016-11-01 00:00:00

  • Nanoparticle-mediated down-regulation of TWIST increases radiosensitivity of nasopharyngeal carcinoma cells via ERK pathway.

    abstract::Evidence suggests that over-expression of TWIST, an epithelial-mesenchymal transition inducer, might have a correlation with cancer progression and chemoresistance. However, its roles in radioresistance of cancer have rarely been reported. High TWIST expression was detected in nasopharyngeal carcinoma (NPC) and associ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Zhuo X,Chang A,Huang C,Yang L,Zhao H,Wu Y,Zhou Q

    更新日期:2015-03-15 00:00:00

  • Oxymatrine enhanced anti-tumor effects of Bevacizumab against triple-negative breast cancer via abating Wnt/β-Catenin signaling pathway.

    abstract::Bevacizumab, a monoclonal antibody targeting vascular endothelial growth factor A (VEGF-A), was used in combination with traditional chemotherapy as the first line treatment for metastatic colorectal cancer (mCRC), non-small cell lung cancer (NSCLC) and advanced ovarian cancer. However, it shows limited efficacy for h...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Xie W,Zhang Y,Zhang S,Wang F,Zhang K,Huang Y,Zhou Z,Huang G,Wang J

    更新日期:2019-08-01 00:00:00

  • Increased expression of iASPP correlates with poor prognosis in FIGO IA2-IIA cervical adenocarcinoma following a curative resection.

    abstract:BACKGROUND:The function of iASPP (inhibitory member of the ASPP family) in cervical adenocarcinoma remains unknown. The aim of this study was to explore the expression and clinical relevance of iASPP in early stage cervical adenocarcinoma. Methods The clinical data of 75 patients with FIGO stage IA2-IIA cervical adenoc...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Kong F,Shi X,Li H,Li P,Yu J,Li X,Chen J,Sun Y,Jia Y

    更新日期:2015-02-15 00:00:00

  • HOXD-AS1 promotes cell proliferation, migration and invasion through miR-608/FZD4 axis in ovarian cancer.

    abstract::Evidence is accumulating that long non-coding RNAs (lncRNAs) exert crucial roles in the incidence and progression of tumors. HOXD cluster antisense RNA 1 (HOXD-AS1), a cancer-related lncRNA, has been frequently reported to be involved in tumorigenesis and dysregulated in multiple types of human cancers; however, littl...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Wang Y,Zhang W,Wang Y,Wang S

    更新日期:2018-01-01 00:00:00

  • Regulation of PD-L1 expression in cancer and clinical implications in immunotherapy.

    abstract::PD-1/PD-L1 immune checkpoint blockade therapy has become an effective method for the treatment of cancers in the clinic. It has great clinical advantages and therapeutic effects in the treatment of various cancers. However, a considerable number of cancer patients currently have relatively low response rates and drug ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Ju X,Zhang H,Zhou Z,Wang Q

    更新日期:2020-01-01 00:00:00

  • Over expression of hyaluronan promotes progression of HCC via CD44-mediated pyruvate kinase M2 nuclear translocation.

    abstract::Hyaluronan is expressed in hepatocellular carcinoma (HCC) as HCC generally arises from a cirrhotic liver in which excessive production and accumulation of HA leads to developing cirrhosis. Though it has been suggested HA is involved in progression of HCC, the mechanisms underlying the connection between HA and HCC pro...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Li JH,Wang YC,Qin CD,Yao RR,Zhang R,Wang Y,Xie XY,Zhang L,Wang YH,Ren ZG

    更新日期:2016-01-15 00:00:00

  • TLR4 activation leads to anti-EGFR therapy resistance in head and neck squamous cell carcinoma.

    abstract::Epidermal growth factor receptor (EGFR) is highly expressed in head and neck squamous cell carcinoma (HNSCC) and related to cancer progression. The resistance to anti-EGFR therapy remains a major clinical problem in HNSCC. In this study, we found that TOLL-like receptor 4 (TLR4) was highly expressed in 50% of EGFR ove...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Ju H,Hu Z,Lu Y,Wu Y,Zhang L,Wei D,Guo W,Xia W,Liu S,Ren G,Hu J

    更新日期:2020-02-01 00:00:00

  • TDP-43 induces EMT and promotes hepatocellular carcinoma metastasis via activating Wnt/β-catenin signaling pathway.

    abstract::The trans-activation response DNA-binding protein of 43 kDa (TDP-43) is a nuclear protein that has been shown to be involved in the growth and metastasis of breast cancer, neuroblastoma, and melanoma. However, the effect of TDP-43 on hepatocellular carcinoma (HCC) metastasis remains unclear. Here, we demonstrated that...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Guo F,Wang H,Jiang M,Yang Q,Xiang Q,Zhou H,Hu X,Hao K,Yang J,Cao H,Shen Z

    更新日期:2020-10-01 00:00:00

  • Overexpression and oncogenic function of HMGA2 in endometrial serous carcinogenesis.

    abstract::The high-mobility group A protein 2 (HMGA2) is a non-histone chromatin factor highly expressed in fetal tissue and malignant tumors but rarely detected within normal adult tissues. The clinical implications and biological functions of HMGA2 in endometrial carcinoma are largely unknown. Here we report that HMGA2 expres...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Wei L,Liu X,Zhang W,Wei Y,Li Y,Zhang Q,Dong R,Kwon JS,Liu Z,Zheng W,Kong B

    更新日期:2016-01-15 00:00:00

  • Long noncoding RNA NBAT1 negatively modulates growth and metastasis of osteosarcoma cells through suppression of miR-21.

    abstract::Osteosarcoma (OS) is the most common primary bone malignancies. Long noncoding RNAs (lncRNAs) are key regulatory RNAs which takes part in several biological processes. LncRNA neuroblastoma associated transcript 1 (NBAT1) is a newly identified functional lncRNA. NBAT1 functions as a tumor suppressor in some cancers. Ho...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Yang C,Wang G,Yang J,Wang L

    更新日期:2017-10-01 00:00:00

  • Serum glycoprotein-derived N- and O-linked glycans as cancer biomarkers.

    abstract::Early detection of cancer is the key to improving survival. Since most clinically used serum cancer biomarkers are either glycoproteins or glycan structures that can be recognized by specific monoclonal antibodies, developing glycan structure-based biomarkers from human serum/plasma glycoproteins through mass spectrom...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Lan Y,Hao C,Zeng X,He Y,Zeng P,Guo Z,Zhang L

    更新日期:2016-11-01 00:00:00

  • MiR-449a suppresses cell invasion by inhibiting MAP2K1 in non-small cell lung cancer.

    abstract::Increasing evidence reveals that deregulation of miRNAs contributes to carcinogenesis of the human non-small cell lung cancer (NSCLC). Our study discovered that the expression of miR-449a was markedly decreased in NSCLC cells with high metastatic capacity and tissues of positive lymph node metastasis. Moreover, our re...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: You J,Zhang Y,Li Y,Fang N,Liu B,Zu L,Zhou Q

    更新日期:2015-08-15 00:00:00

  • MiR-26a inhibits thyroid cancer cell proliferation by targeting ARPP19.

    abstract::miRNA, which involves in pathogenesis of thyroid cancer via different targets, has been found aberrantly expressed in thyroid cancer. Modes of actions of miR-26a in papillary thyroid carcinoma (PTC), however, have not been fully understood to this date. In vitro results obtained from this research confirmed miR-26a wa...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Gong Y,Wu W,Zou X,Liu F,Wei T,Zhu J

    更新日期:2018-06-01 00:00:00

  • Endoscopic submucosal dissection versus esophagectomy for early esophageal squamous cell carcinoma with tumor invasion to different depths.

    abstract::Endoscopic submucosal dissection (ESD) is a minimally invasive alternative to esophagectomy for early esophageal squamous cell carcinoma (EESCC). The aim of this study was to compare the efficacy and safety of ESD and esophagectomy in EESCC with different depth of invasion. The data of EESCC patients who received ESD ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: An W,Liu MY,Zhang J,Cui YP,Gao J,Wang LP,Chen Y,Yang LX,Chen HZ,Jin H,Liu F,Chen J,Li ZS,Wang LW,Shi XG,Sun C

    更新日期:2020-09-01 00:00:00

  • Elevated RABEX-5 expression predicts poor prognosis in non-small-cell lung cancer.

    abstract::RABEX-5 has been studied in various solid tumors, but its role in non-small-cell lung cancer (NSCLC) remains unknown. This study is aimed to investigate the expression, the potential relevance to clinicopathological characters and prognostic significance of RABEX-5 in patients with NSCLC. A total of 120 NSCLC patients...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Zhang F,Jia Y,Kong F,Hu G,Cai Q,Xu T

    更新日期:2015-08-15 00:00:00

  • SLFN5 influences proliferation and apoptosis by upregulating PTEN transcription via ZEB1 and inhibits the purine metabolic pathway in breast cancer.

    abstract::Human Schlafen-5 (SLFN5) is aberrantly involved in tumorigenesis in several types of cancer. However, its implications in breast cancer (BRCA) are unknown. Herein, we demonstrated that SLFN5 expression is negatively associated with the tumour growth of human BRCA using GEO database analysis and clinical sample immunos...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Gu X,Wan G,Yang Y,Liu Y,Yang X,Zheng Y,Jiang L,Zhang P,Liu D,Zhao W,Huang G,Lu C

    更新日期:2020-09-01 00:00:00

  • Enhancing chemotherapy sensitivity by targeting PcG via the ATM/p53 pathway.

    abstract::Histone modification and chromatin remodeling are important events in response to DNA damage, and Polycomb group (PcG) proteins, catalyzing H3K27 methylation, are involved. However, the biological function and mechanism of PcG in DNA damage are not fully understood. Additionally, downstream effectors in hepatocellular...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Gao SB,Li KL,Qiu H,Zhu LY,Pan CB,Zhao Y,Wei SH,Shi S,Jin GH,Xue LX

    更新日期:2017-09-01 00:00:00

  • Resveratrol-mediated ADAM9 degradation decreases cancer progression and provides synergistic effects in combination with chemotherapy.

    abstract::Metastasis is a crucial hallmark of cancer progression and remains the primary cause of patient deaths. Metastasis-associated proteases contribute to cancer progression by disrupting the extracellular matrix interaction to facilitate the spreading of cancer cells to other organs. ADAM9, a type of metalloprotease, has ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Lin YS,Hsieh CY,Kuo TT,Lin CC,Lin CY,Sher YP

    更新日期:2020-11-01 00:00:00

  • Long non-coding RNA LINC00673 promotes breast cancer proliferation and metastasis through regulating B7-H6 and epithelial-mesenchymal transition.

    abstract:BACKGROUND:Long non-coding RNAs (LncRNAs) have been reported as key regulators of tumor progression in recent decades. However, the potential molecular mechanisms of breast cancer are still unclear. With the development of sequencing technology, we discovered that LINC00673 is upregulated in tumor tissues. But the main...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Xia E,Shen Y,Bhandari A,Zhou X,Wang Y,Yang F,Wang O

    更新日期:2018-07-01 00:00:00

  • Dehydrocostus lactone, a natural sesquiterpene lactone, suppresses the biological characteristics of glioma, through inhibition of the NF-κB/COX-2 signaling pathway by targeting IKKβ.

    abstract::Dehydrocostus lactone (DHE), a natural sesquiterpene lactone, has been used for treatment of various diseases with its anti-inflammatory activity. Recently, it has caused extensive interest in researchers due to it has anti-cancer abilities in some types of carcinomas. However, the anti-cancer effect and mechanism of ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Wang J,Yu Z,Wang C,Tian X,Huo X,Wang Y,Sun C,Feng L,Ma J,Zhang B,Yang Q,Ma X,Xu Y

    更新日期:2017-06-01 00:00:00

  • Advances in malignant pleural mesothelioma therapy: targeting EphA2 a novel approach.

    abstract::Malignant pleural mesothelioma (MPM) is an aggressive neoplasm with a poor prognosis. MPM grows from the mesothelial cells lining the surface of the lung and chest wall called Pleura. Exposure to asbestos is mainly linked to the development of MPM. Approximately 80% of the tumors are pleural in origin, and up to 3000 ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Nasreen N,Khodayari N,Mohammed KA

    更新日期:2012-01-01 00:00:00

  • Rottlerin exhibits anti-cancer effect through inactivation of S phase kinase-associated protein 2 in pancreatic cancer cells.

    abstract::Rottlerin, a natural product isolated from Mallotus philippinensis, has been characterized as an effective chemoprevention agent in inhibiting tumor cell growth. Although multiple studies have revealed the role of rottlerin in tumorigenesis, the molecular mechanism of rottlerin-mediated anti-tumor activity has not bee...

    journal_title:American journal of cancer research

    pub_type: 杂志文章

    doi:

    authors: Su J,Wang L,Yin X,Zhao Z,Hou Y,Ye X,Zhou X,Wang Z

    更新日期:2016-10-01 00:00:00

  • Aberrant miR-1246 expression promotes radioresistance in non-small cell lung cancer: a potential prognostic biomarker and radiotherapy sensitization target [Retraction].

    abstract::[This retracts the article on p. 314 in vol. 10, PMID: 32064170.]. ...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,撤回出版物

    doi:

    authors:

    更新日期:2020-08-01 00:00:00

  • METTL3 plays multiple functions in biological processes.

    abstract::N6-methyladenosine (m6A) is the most common internal modification of mRNAs in higher eukaryotic. This process is performed by methyltransferase. Methyltransferase-like 3 (METTL3) is the best known m6A methyltransferase that functions in the reversible epi-transcriptome modulation of m6A modification. Besides acting as...

    journal_title:American journal of cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Liu S,Zhuo L,Wang J,Zhang Q,Li Q,Li G,Yan L,Jin T,Pan T,Sui X,Lv Q,Xie T

    更新日期:2020-06-01 00:00:00