Abstract：
OBJECTIVE:Osteoarthritis (OA) is a prevalent degenerative disease caused by various factors. MicroRNAs are important regulators in OA. MiR-21-5p expression is decreased in OA cartilage, but the effects of modulating miR-21-5p on cartilage regeneration are unknown. Therefore, our aim was to investigate the effects of miR-21-5p on cartilage metabolism of OA chondrocytes. DESIGN:We used IL-1β (10 ng/ml) to mimic OA chondrocytes. OA chondrocytes were transfected with miR-21-5p, the gene expression of COL2A1, MMP13, and ADAMTS5 was detected by qPCR. At the same time, COL2A1, MMP13, and ADAMTS5 were analyzed at the protein level by Western blot. CCK8 measured the cell's viability and SA-β-gal detected the cell's senescence. RESULTS:Upregulation of miR-21-5p had increased COL2A1 expression and decreased MM P13 and ADAMTS5 expression, which were in accord with Western blot data. SA-β-gal activity significantly increased, the viability was decreased in OA chondrocytes, and upregulation of miR-21-5p can decrease the SA-β-gal activity and increase cell viability. CONCLUSION:MiR-21-5p might be a potential disease-modifying compound in OA, as it promotes hyaline cartilage production. These results provided that novel insights into the important function in OA pathological development.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Zhu H,Yan X,Zhang M,Ji F,Wang Sdoi
10.1186/s13018-019-1160-7subject
Has Abstractpub_date
2019-05-03 00:00:00pages
118issue
1issn
1749-799Xpii
10.1186/s13018-019-1160-7journal_volume
14pub_type
杂志文章abstract：BACKGROUND:The purpose of this study was to introduce arthroscopic partial trapeziectomy and tendon interposition for the treatment of symptomatic thumb carpometacarpal arthritis of Eaton stage II or III. METHODS:From August 2001 to April 2009, 23 patients with thumb carpometacarpal arthritis were treated using this t...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0329-y
更新日期：2015-12-18 00:00:00
abstract：OBJECTIVE:An evaluation of safety and efficacy of high molecular weight hyaluronan (HA) delivered at the time of arthroscopic debridement of the osteoarthritic knee. METHODS:Thirty consecutive patients who met inclusion and exclusion criteria underwent arthroscopic debridement by a single surgeon and concomitant deliv...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-3-43
更新日期：2008-09-17 00:00:00
abstract：BACKGROUND:Mesenchymal stem cells (MSCs) have great potential for the repair and regeneration of bone fracture, but their optimal origins remain controversial. METHODS:Bone marrow-MSCs (BM-MSCs) and bone-bone marrow-MSCs (B-BM-MSCs) were isolated from 12 SD rats, and the morphology, MSC-associated markers, and prolife...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1346-z
更新日期：2019-09-03 00:00:00
abstract：BACKGROUND:Nasal Staphylococcus aureus (S. aureus) screening and decolonization has been widely used to reduce surgical site infections (SSIs) prior to total knee and hip arthroplasty (TKA and THA). However, it remains considerably controversial. The aim of this study was to ascertain whether this scheme could reduce S...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,meta分析
doi：10.1186/s13018-020-01601-0
更新日期：2020-02-19 00:00:00
abstract：BACKGROUND:When performing arthroscopic ankle arthrodesis for end-stage ankle arthritis, internal fixation is performed using bone screws after appropriate preparation. However, optimal characteristics of bone screws have not been examined in terms of pressure force. Objective comparisons of bone-screw performance may ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-016-0503-x
更新日期：2017-01-17 00:00:00
abstract：INTRODUCTION:Knee extension deficit or loss of extension (LOE) is a potential complication following ACL reconstruction (ACLR); however, the change in postoperative knee extension during rehabilitation is not well defined. The aim of this review is to establish the trajectory of knee extension recovery and incidence of...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1127-8
更新日期：2019-03-28 00:00:00
abstract：BACKGROUND:Endoscopic curettage is considered applicable for the treatment of simple bone cysts with the expectation that it might be less invasive than open curettage. In this study, we investigated the efficacy of endoscopic curettage for the treatment of simple bone cysts. The goal was to investigate the incidence o...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0869-z
更新日期：2018-07-05 00:00:00
abstract：BACKGROUND:Closed reduction and percutaneous fixation are considered as the optional treatments for displaced supracondylar humerus fractures. However, there was no published report about the biomechanical analysis in Orthofix® external fixator. In this study, we developed a model of supracondylar humerus fractures and...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0893-z
更新日期：2018-07-28 00:00:00
abstract：OBJECTIVES:The aim of this study was to evaluate the efficacy of local infiltration anaesthesia (LIA) during primary total hip arthroplasty (THA) via a posterolateral approach under general anaesthesia and to compare the efficacy of LIA in all layers with LIA in the deep and superficial fascia. PATIENTS AND METHODS:On...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-02182-8
更新日期：2021-01-22 00:00:00
abstract：OBJECTIVES:To investigate clinical effects and manual operational point of Bryan cervical disc prosthesis in Chinese, to observe the stability and range of movement (ROM) post-operatively. METHODS AND MATERIALS:From 2003,12 to 2005,12, Bryan disc prosthesis replacement applied in 83 cases (102 levels) of cervical spon...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-1-13
更新日期：2006-11-04 00:00:00
abstract：BACKGROUND:Intervertebral disk degeneration (IDD) is a degenerative disease characterized by cytoplasm loss and extracellular matrix degradation. Numerous evidence reported that miRNAs participated in IDD development. Nevertheless, the function of miR-142-3p in IDD development remains unknown. This study mainly explore...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-02194-4
更新日期：2021-01-14 00:00:00
abstract：BACKGROUND:In recent years, the modified Dunn osteotomy has gained popularity to treat slipped capital femoral epiphysis (SCFE) with various complication rates. Most studies included patients with different severities. This study aimed to determine (1) the radiological and clinical outcome, (2) the health-related quali...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1433-1
更新日期：2019-11-08 00:00:00
abstract：BACKGROUND:Acetabular orientation changes after periacetabular osteotomy (PAO) lead to technical change when performing subsequent total hip arthroplasty (THA). There is no unified consensus regarding the solution for acetabular component installation after PAO. In the current study, we performed computed tomography (C...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-02001-0
更新日期：2020-10-29 00:00:00
abstract：BACGROUND:Although the contribution of fibroblast growth factor (FGF)-2 and mesenchymal stromal cells (MSCs) to bone formation is well known, few studies have investigated the combination of an autologous bone graft with FGF-2 or MSCs for large bone defects. METHODS:We studied an atrophic non-union model with a large ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-016-0442-6
更新日期：2016-09-26 00:00:00
abstract：:Over the years, management of complex distal radius fractures by closed means has often failed leading to late collapse. We have chosen the principle of ligamentotaxis using external fixation and bone grafting in this study to prevent late complications. Eighty one patients with complex distal radius fractures belongi...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-6-23
更新日期：2011-05-21 00:00:00
abstract：BACKGROUND:Joints withstand huge forces, but little is known about subchondral pressures and perfusion during loading. We developed an in vitro calf foot model to explore intraosseous pressure (IOP) and subchondral perfusion during weight bearing. METHODS:Freshly culled calf forefeet were perfused with serum. IOP was ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01754-y
更新日期：2020-06-29 00:00:00
abstract：PURPOSE:To compare the specificity and sensitivity of preoperative D-dimer and age-adjusted D-dimer value for predicting the incidence of the DVT preoperatively in total joint arthroplasty (TJA) patients. METHODS:We enrolled 406 patients finally above 50 years old. Everyone had done ultrasonography bedside, and D-dime...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-02172-w
更新日期：2021-01-25 00:00:00
abstract：BACKGROUND:Biological reconstruction surgery is a tough but alluring option for treating primary malignant musculoskeletal tumors. In this article, we evaluate the clinical outcomes of primary malignant musculoskeletal tumors treated with inactivated autograft using alcohol. METHOD:In this article, we include 58 patie...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0324-3
更新日期：2015-11-17 00:00:00
abstract：BACKGROUND:Lubrication function is impaired and the lubricant content of synovial fluid (SF) changes immediately after plateau tibia fractures. Here, we aimed to analyze the lubricant content of SF at chronic term following plateau tibia fracture. METHODS:Forty-eight surgically treated patients without joint incongrue...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0232-6
更新日期：2015-06-03 00:00:00
abstract：BACKGROUND:Hospital-acquired infections (HAIs) are an emerging global problem that increases in-hospital mortality, length of stay, and cost. We performed a 6-year retrospective study to provide valuable insight into appropriate antibiotic use in HAI cases. We also aimed to understand how hospitals could reduce pathoge...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-021-02234-7
更新日期：2021-02-01 00:00:00
abstract：BACKGROUND:The purpose of the current study was to examine the effect of a non-invasive, home-based biomechanical treatment program for patients with spontaneous osteonecrosis of the knee (SONK). METHODS:Seventeen patients with SONK, confirmed by MRI, participated in this retrospective analysis. Patients underwent a s...
journal_title：Journal of orthopaedic surgery and research
pub_type： 临床试验,杂志文章
doi：10.1186/s13018-016-0472-0
更新日期：2016-11-14 00:00:00
abstract：BACKGROUND:Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) could provide valuable findings for tendon regeneration. A non-invasive image method that can effectively evaluate the quality of the scar tissue has not yet been employed. METHODS:Thirteen New Zealand rabbits were divided into two groups: group...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-014-0132-1
更新日期：2014-12-12 00:00:00
abstract：BACKGROUND:The aim of this study was to analyze the pattern and types of sensory nerve endings in ankle collateral ligaments using histological techniques, in order to observe the morphology and distribution of mechanoreceptors in the collateral ligaments of cadaver ankle joint, and to provide the morphological evidenc...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0215-7
更新日期：2015-06-12 00:00:00
abstract：BACKGROUND:Long-term results of the 'classic' low contact stress (LCS) total knee replacement (TKR) have been satisfactory; nonetheless, design changes have been made which resulted in the 'complete' LCS TKR. The aim of this study is to compare the 5-year incidence of revision and midterm clinical performance before an...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-9-7
更新日期：2014-02-06 00:00:00
abstract：BACKGROUND:Open reduction with internal fixation (ORIF) and percutaneous surgery (PS) are the most common surgical procedures for the treatment of displaced intra-articular calcaneal fractures. The purpose of this retrospective study was to compare the clinical and radiological results of these techniques and to verify...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-016-0426-6
更新日期：2016-08-22 00:00:00
abstract：INTRODUCTION:The aim of this study was to determine the differences and consistencies in the morphological and angular interpretations of standard USG images. Therefore, it was aimed to show the correlations of orthopaedic doctors with different periods of experience in hip ultrasound measurements taken with the Graf m...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1263-1
更新日期：2019-07-17 00:00:00
abstract：BACKGROUND:The objectives of this study are to investigate the clinical curative effect of Gallie technique and atlantoaxial screw-rod constructs (SRC) on atlantoaxial sagittal instability and determine the indication of Gallie technique. METHODS:Data of 49 patients with atlantoaxial sagittal instability from February...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-017-0607-y
更新日期：2017-07-11 00:00:00
abstract：BACKGROUND:This randomized controlled trial was carried out to (1) evaluate the effect of nucleoplasty with coblation on the PLA2 activity in the degenerative intervertebral disks of an animal model and (2) explore the possible therapeutic mechanism of coblation in addition to the current theory, which focuses on decre...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0175-y
更新日期：2015-03-25 00:00:00
abstract：BACKGROUND:This systematic review and meta-analysis assessed the role of nonoperative treatment and volar locking plate (VLP) fixation in elderly patients with distal radial fracture. METHODS:The systematic literature review identified randomized controlled trials (RCTs) and observational studies using VLP and nonoper...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,评审
doi：10.1186/s13018-020-01734-2
更新日期：2020-07-14 00:00:00
abstract：BACKGROUND:Graft shrinkage or radial extrusion is a reported complication after meniscus allograft transplantation (MAT). Whether shrinkage or extrusion progress after surgery and whether they are associated with the clinical outcome of MAT remain debatable. In this study, graft shrinkage and extrusion were measured in...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0892-0
更新日期：2018-07-20 00:00:00
