Abstract：
BACKGROUND:Intervertebral disk degeneration (IDD) is a degenerative disease characterized by cytoplasm loss and extracellular matrix degradation. Numerous evidence reported that miRNAs participated in IDD development. Nevertheless, the function of miR-142-3p in IDD development remains unknown. This study mainly explored the potential role and function of miR-142-3p in IDD development. METHODS:One percent fetal bovine serum was used to induce the degeneration of ATDC5 cells, and miR-142-3p level was examined by qRT-PCR. Then, miR-142-3p mimic/inhibitor and its corresponding negative control were transfected into ATDC5 normal and degenerative cells. Viability, migration, invasion, apoptosis, cycle, Bax, Bcl-2, P62, and Beclin1 expression levels were assessed using CCK8, wound healing assay, annexin V-FITC/PI staining, western blot, and qRT-PCR, respectively. RESULTS:The results revealed that the expression levels of MMP13, ADAMTS5, MMP3, and Col-X were increased as well as the expression levels of SOX-9 and Col-II were reduced in ATDC5 degenerative cells, indicating the degeneration model was constructed. We observed that miR-142-3p was decreased in ATDC5 degenerative cells and its suppression could promote ATDC5 cell degeneration. However, miR-142-3p overexpression could reverse the cell viability inhibition, as well as apoptosis and autophagy enhancement in ATDC5 degenerative cells. CONCLUSIONS:Our results proved that miR-142-3p may play an important role in disk degeneration. Further animal study is needed to illustrate the role of the miR-142-3p in IDD development.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Xue J,Hu B,Xing W,Li F,Huang Z,Zheng W,Wang B,Zhu Y,Yang Xdoi
10.1186/s13018-020-02194-4subject
Has Abstractpub_date
2021-01-14 00:00:00pages
55issue
1issn
1749-799Xpii
10.1186/s13018-020-02194-4journal_volume
16pub_type
杂志文章abstract：BACKGROUND:The medial collateral ligament (MCL) is the main static stabilizer of the medial knee. The surgical treatment was recommended in cases with serious medial collateral ligament insufficiency combined with multi-ligament injuries and chronic symptomatic medial instability. Several surgical techniques have been ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-016-0416-8
更新日期：2016-07-22 00:00:00
abstract：BACKGROUND:The scarf osteotomy is a well-established surgical method for correcting a hallux valgus deformity. It is often combined with an Akin osteotomy. However, clear guidelines defining indication criteria are missing. The purpose of this study was to analyze the radiological outcome after scarf osteotomy in depen...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1241-7
更新日期：2019-06-26 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-016-0461-3
更新日期：2016-10-24 00:00:00
abstract：:Following publication of the original article [1], we have been notified that there was a spelling mistake in the author affiliations. In this Correction, correct and incorrect versions are shown. ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,已发布勘误
doi：10.1186/s13018-020-01680-z
更新日期：2020-04-30 00:00:00
abstract：:We present a case report of a free thenar flap surgery done for a volar right hand middle finger, distal and middle phalanx degloving injury. A free thenar flap is a fasciocutaneous sensate flap supplied by a constant branch of the superficial radial artery and its variable nerve supply. It has a distinct advantage of...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-2-4
更新日期：2007-03-12 00:00:00
abstract：BACKGROUND:Although internal fixation has been the main treatment option for elderly patients with an undisplaced femoral neck fracture, it is associated with a high reoperation rate. Some surgeons have discussed the use of hemiarthroplasty, but there is limited literature comparing these two treatment modalities. In t...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,meta分析
doi：10.1186/s13018-019-1377-5
更新日期：2019-10-11 00:00:00
abstract：: Tuberculosis of symphysis pubis is a rare condition with hardly any report of such cases in the last decade. It is necessary to distinguish the entity from more common ones like Osteitis pubis and Osteomyelitis of pubis symphysis by urgent means in order to start the treatment early and thereby minimize morbidity and...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-5-63
更新日期：2010-08-27 00:00:00
abstract：BACKGROUND:The optimal treatment of osteonecrosis of the femoral head has not been established yet. The aim of this study was to report preliminary clinical results of focal anatomic-resurfacing implantation for the treatment of osteonecrosis of the femoral head. METHODS:Five patients (four male, one female) with seve...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0199-3
更新日期：2015-04-28 00:00:00
abstract：BACKGROUND:In total knee arthroplasty (TKA) a flexible intramedullary rod can be used to account for sagittal bowing of the distal femur. Although patients report better post-operative functional outcome when the flexible rod was used, it is unknown how the use of the flexible rod affects the placement of the femoral T...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01989-9
更新日期：2020-10-14 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1160-7
更新日期：2019-05-03 00:00:00
abstract：BACKGROUND:Posterior transfacet approach has been proved to be a safe and effective access to treat thoracic disc herniation. However, the therapeutic effect and safety of modified transfacet approach for treating thoracic spinal tuberculosis (TST) has not been reported in the clinical literature. In this study, the cl...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0994-8
更新日期：2018-11-20 00:00:00
abstract：BACKGROUND:Intertrochanteric fracture (ITF) is increasing with the rapid increase in the aging population, often causes a high mortality rate in old patients and increases the economic burden of the family and society. ERAS (Enhanced Recovery after Surgery) is a powerful guarantee for patients to accelerate their recov...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,随机对照试验
doi：10.1186/s13018-020-01586-w
更新日期：2020-03-05 00:00:00
abstract：OBJECTIVE:To discuss the sensitivity and specificity of the combinations of multiple factors that work on bone infection after artificial joint, and provide evidence-based medical basis for the early diagnosis of infection after artificial joint. METHODS:A retrospective review was conducted on 35 patients diagnosed wi...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01669-8
更新日期：2020-04-25 00:00:00
abstract：BACKGROUND:This study aimed to investigate the effects of silk fiber (SF)/calcium phosphate cement (CPC) biocomposite on repairing radial bone defects in rabbits. METHODS:Four-month-old New Zealand rabbits were selected to create a bilateral radial bone defect model and divided into four groups according to implanted ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-017-0529-8
更新日期：2017-02-21 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-016-0388-8
更新日期：2016-04-19 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01638-1
更新日期：2020-03-31 00:00:00
abstract：BACKGROUND:The purpose of this study was to investigate the relationship between the bone length available for coracoid transfer without coracoclavicular ligament injury and the distance from the coracoid tip to the attachments of the coracoacromial ligament or pectoralis minor. We hypothesized that cadaver height and ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1212-z
更新日期：2019-06-10 00:00:00
abstract：BACKGROUND:Using a larger, more comprehensive sample, and inclusion of the reverse shoulder arthroplasty as a primary surgical approach for proximal humerus fracture, we report on geographic variation in the treatment of proximal humerus fracture in 2011 and comment on whether treatment consensus is being reached. MET...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-1052-2
更新日期：2019-01-21 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01885-2
更新日期：2020-08-24 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-3-43
更新日期：2008-09-17 00:00:00
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journal_title：Journal of orthopaedic surgery and research
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doi：10.1186/s13018-020-01828-x
更新日期：2020-08-06 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0801-6
更新日期：2018-05-03 00:00:00
abstract：BACKGROUND:Sternoclavicular joint (SCJ) infectious arthritis is a rare disease. A standard treatment for SCJ infection has not been established. This study aimed to assess the clinical outcomes of a three-stage procedure with bone transportation (BT) for treating SCJ infectious arthritis. METHODS:Six patients (mean ag...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-016-0480-0
更新日期：2016-11-25 00:00:00
abstract：BACKGROUND:This meta-analysis aims to illustrate the efficacy and safety of intrathecal morphine (ITM) versus femoral nerve block (FNB) for pain control after total knee arthroplasty (TKA). METHODS:In April 2017, a systematic computer-based search was conducted in PubMed, EMBASE, Web of Science, Cochrane Database of S...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,meta分析
doi：10.1186/s13018-017-0621-0
更新日期：2017-08-16 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 临床试验,杂志文章
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更新日期：2019-12-02 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1433-1
更新日期：2019-11-08 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-017-0557-4
更新日期：2017-04-08 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1198-6
更新日期：2019-06-07 00:00:00
abstract：BACKGROUND:Long-term success reports by Dr. Ponseti with the Ponseti method in the treatment of congenital idiopathic clubfoot have led to a renewed interest in this method among pediatric orthopedists. The purpose of this study is to evaluate mid-term effectiveness of Ponseti method for the treatment of congenital idi...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-6-3
更新日期：2011-01-12 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-4-2
更新日期：2009-01-30 00:00:00
