Abstract:
BACKGROUND:Mesenchymal stem cells (MSCs) have great potential for the repair and regeneration of bone fracture, but their optimal origins remain controversial. METHODS:Bone marrow-MSCs (BM-MSCs) and bone-bone marrow-MSCs (B-BM-MSCs) were isolated from 12 SD rats, and the morphology, MSC-associated markers, and proliferative capacity of these cells were compared using an inverted microscope, flow cytometry, and CCK-8 assays, respectively. After 14 days of osteoblastic induction, osteoblast phenotypes were detected by ALP and calcium nodule staining, and the expression of BMP-2 and TGF-β1 was observed by western blotting. Then, the rat tibia fracture model was established with 3 groups (n = 6 per group), the control, BM-MSC, and B-BM-MSC groups. Computed tomography (CT) imaging was performed to evaluate fracture healing at weeks 2, 4, and 6. Finally, the fractured bones were removed at weeks 4 and 6, and HE staining was performed to evaluate fracture healing. RESULTS:Although the 2 types of MSCs shared the same cellular morphology and MSC-associated markers, B-BM-MSCs had a higher proliferative rate than BM-MSCs from day 9 to day 12 (p < 0.05), and the expression levels of ALP and calcium were obviously higher in B-BM-MSCs than in BM-MSCs after osteogenic induction (p < 0.01 and p < 0.001, respectively). Western blot results showed that the expression levels of BMP-2 and TGF-β1 in B-BM-MSCs were higher than in BM-MSCs before and after osteogenic induction (p < 0.01). In the animal experiments, CT imaging and gross observation showed that B-BM-MSCs had a greater capacity than BM-MSCs to promote fracture healing, as the Lane-Sandhu scores of B-BM-MSCs at weeks 4 and 6 after operation (3.00 ± 0.81 and 9.67 ± 0.94, respectively) were higher than those of BM-MSCs (1.33 ± 0.47 and 6.67 ± 1.25, respectively; both p < 0.05). The HE staining results further supported this conclusion. CONCLUSIONS:Taken together, our study results proved that MSCs obtained by co-culturing the bone and bone marrow from SD rats had better proliferative, osteogenic differentiation, and fracture healing capacities than BM-MSCs, perhaps suggesting a novel way to obtain MSCs for bone tissue repair.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Zhu C,Sha M,Jiang H,Lin J,Lin W,Li W,Chen X,Huang G,Ding Zdoi
10.1186/s13018-019-1346-zsubject
Has Abstractpub_date
2019-09-03 00:00:00pages
293issue
1issn
1749-799Xpii
10.1186/s13018-019-1346-zjournal_volume
14pub_type
杂志文章abstract:PURPOSE:The aim of this retrospective observational study of one cohort was to evaluate the long-term outcome in patients younger than 60 years after total hip arthroplasty using a straight uncemented stem and an uncemented threaded cup. METHODS:Between 1986 and 1987, 75 hips of 75 patients (mean age, 53.35 ± 6.17 yea...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-02102-w
更新日期:2020-11-26 00:00:00
abstract:BACKGROUND:The aim of this study was to analyze the pattern and types of sensory nerve endings in ankle collateral ligaments using histological techniques, in order to observe the morphology and distribution of mechanoreceptors in the collateral ligaments of cadaver ankle joint, and to provide the morphological evidenc...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-015-0215-7
更新日期:2015-06-12 00:00:00
abstract:BACKGROUND:By comparing clinical outcomes between dual plating (DP) and exchange nailing with augmentation plating (EN/AP), we aimed to provide better treatment strategies for femoral shaft non-union. METHODS:We retrospectively reviewed 30 patients with aseptic femoral shaft non-union at our level 1 trauma center betw...
journal_title:Journal of orthopaedic surgery and research
pub_type: 临床试验,杂志文章
doi:10.1186/s13018-018-1002-z
更新日期:2018-11-20 00:00:00
abstract::The von Willebrand Factor A (vWF A) domain is one of the most widely distributed structural modules in cell-matrix adhesive molecules such as intergrins and extracellular matrix proteins. Mutations in the vWF A domain of matrilin-3 cause multiple epiphyseal dysplasia (MED), however the pathological mechanism remains t...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-3-21
更新日期:2008-06-02 00:00:00
abstract:BACKGROUND:Retrospective analysis of the characteristics and correlation of spino-pelvic sagittal parameters in elderly patients with lumbar degenerative disease. METHODS:Eighty-seven patients with lumbar degenerative disease, with an average age of 75.4 years old, were enrolled in the observation group. They were all...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1156-3
更新日期:2019-05-09 00:00:00
abstract:BACKGROUND:Tibial plateau fracture (TPF) includes different fracture patterns with varied degrees of articular depression and displacement. Many kinds of fixators, including newly designed plate with locking screws, were applied to treat these complicated fractures. We intended to follow up the surgical outcomes of (1)...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-014-0062-y
更新日期:2014-07-20 00:00:00
abstract:BACKGROUND:Long-term results of the 'classic' low contact stress (LCS) total knee replacement (TKR) have been satisfactory; nonetheless, design changes have been made which resulted in the 'complete' LCS TKR. The aim of this study is to compare the 5-year incidence of revision and midterm clinical performance before an...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-9-7
更新日期:2014-02-06 00:00:00
abstract:BACKGROUND:Posterior tibial tendon (PTT) insufficiency is considered as the main cause of adult acquired flat foot and is three times more frequent in females. High estrogen levels exert a positive effect on the overall collagen synthesis in tendons. We have previously demonstrated the association between some genetic ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-1020-x
更新日期:2018-12-11 00:00:00
abstract:BACKGROUND:Matrix metalloproteinase (MMPs) synthesized and secreted from connective tissue cells have been thought to participate in degradation of the extracellular matrix. Increased MMPs activities that degrade proteoglycans have been measured in osteoarthritis cartilage. This study aims to suppress the expression of...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-4-45
更新日期:2009-12-24 00:00:00
abstract:BACKGROUND:Although differences in the results of the bone marrow stimulation technique between the knee and ankle have been reported, a detailed mechanism for those differences has not been clarified. The purpose of this study was to examine whether morphological differences between the knee and ankle joint affect the...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0444-4
更新日期:2016-10-04 00:00:00
abstract:BACKGROUND:Excessive blood loss caused by total joint arthroplasty (TJA) often increases the requirement for blood transfusion, which is associated with adverse outcomes. The purpose of this study was to determine the relationship between perioperative transfusion and postoperative DVT in TJA. METHODS:This retrospecti...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1270-2
更新日期:2019-07-23 00:00:00
abstract:BACKGROUND:This study aimed to investigate the incidence and risk factors of postoperative urinary retention (POUR) among elderly patients who underwent hip fracture surgery and to evaluate the effect of indwelling catheterization on the occurrence of POUR. MATERIALS AND METHODS:From January 2012 to January 2015, cons...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1360-1
更新日期:2019-09-18 00:00:00
abstract:BACKGROUND:Using a larger, more comprehensive sample, and inclusion of the reverse shoulder arthroplasty as a primary surgical approach for proximal humerus fracture, we report on geographic variation in the treatment of proximal humerus fracture in 2011 and comment on whether treatment consensus is being reached. MET...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-1052-2
更新日期:2019-01-21 00:00:00
abstract::The use of computer navigation is becoming a well-recognized technical alternative to conventional total knee arthroplasty (TKA). However, computer navigation has a substantial learning curve and the use of commercially available navigation systems increases surgical time. In addition, the potential risks associated w...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-6-26
更新日期:2011-05-26 00:00:00
abstract:BACKGROUND:Lumbar floating fusion occasionally causes postoperative adjacent segment disorder (ASD) at lumbosacral level, causing L5 spinal nerve disorder by L5-S1 foraminal stenosis. The disorder is considered to be one of the major outcomes of L5-S1 ASD, which has not been evaluated yet. The present study aimed to ev...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-015-0307-4
更新日期:2015-10-17 00:00:00
abstract:BACKGROUND:Recently, the excellent outcomes of temporary fixation of C1-2 without fusion in the treatment of odontoid fracture had been reported. It is still unclear if this technique could achieve the equivalent outcomes as the golden standard technique of anterior screw fixation. The objective of this study is to com...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-017-0702-0
更新日期:2018-01-08 00:00:00
abstract:BACKGROUND:It is not clear whether total hip arthroplasty performed via a minimally invasive approach leads to less muscle trauma compared to the standard approach. MATERIALS AND METHODS:To investigate whether a minimally invasive posterior approach for total hip arthroplasty results in lower levels of muscle-derived ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-5-46
更新日期:2010-07-27 00:00:00
abstract:BACKGROUND:When performing arthroscopic ankle arthrodesis for end-stage ankle arthritis, internal fixation is performed using bone screws after appropriate preparation. However, optimal characteristics of bone screws have not been examined in terms of pressure force. Objective comparisons of bone-screw performance may ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0503-x
更新日期:2017-01-17 00:00:00
abstract:BACKGROUND:Arthroscopic rotator cuff repairs are mostly secured with suture anchors and often supplemented by footprint decortication. The objectives of this study were to characterize the strength of bone-tendon healing following anchor repair and assess the effect of channeling the supraspinatus (SSP) humeral footpri...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-0773-6
更新日期:2018-03-27 00:00:00
abstract:: Olecranon fractures are a common injury in fractures. The tension band technique for olecranon fractures yields good clinical outcomes; however, it is associated with significant complications. In many patients, implants irritate overlying soft tissues and cause pain. This is mostly due to protrusion of the proximal ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-5-73
更新日期:2010-10-12 00:00:00
abstract:BACKGROUND:Peripheral nerve stimulation has been used for decades to treat chronic pain but has not been used for postoperative analgesia due to multiple limitations, beginning with invasive electrode placement. With the development of small-diameter/gauge leads enabling percutaneous insertion, ultrasound guidance for ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0506-7
更新日期:2017-01-13 00:00:00
abstract:BACKGROUND:Joints withstand huge forces, but little is known about subchondral pressures and perfusion during loading. We developed an in vitro calf foot model to explore intraosseous pressure (IOP) and subchondral perfusion during weight bearing. METHODS:Freshly culled calf forefeet were perfused with serum. IOP was ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-01754-y
更新日期:2020-06-29 00:00:00
abstract::Ankle sprains are very common injuries seen in the athletic and young population. Majority of patients will improve with a course of rest and physical therapy. However, with conservative management about twenty percent of all patients will go on to develop chronic lateral ankle instability. This manuscript describes o...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-4-41
更新日期:2009-12-02 00:00:00
abstract:BACKGROUND:Biological reconstruction surgery is a tough but alluring option for treating primary malignant musculoskeletal tumors. In this article, we evaluate the clinical outcomes of primary malignant musculoskeletal tumors treated with inactivated autograft using alcohol. METHOD:In this article, we include 58 patie...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-015-0324-3
更新日期:2015-11-17 00:00:00
abstract:BACKGROUND:Cervical sagittal parameters were closely related with clinical outcomes after multi-level ACDF. Our purpose was to evaluate the clinical outcomes and cervical sagittal parameters in patients with MCSM after ACDF and to identify the risk factors of poor clinical outcomes. MATERIAL AND METHODS:ACDF was perfo...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1504-3
更新日期:2019-12-11 00:00:00
abstract:BACKGROUND:Sternoclavicular joint (SCJ) infectious arthritis is a rare disease. A standard treatment for SCJ infection has not been established. This study aimed to assess the clinical outcomes of a three-stage procedure with bone transportation (BT) for treating SCJ infectious arthritis. METHODS:Six patients (mean ag...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0480-0
更新日期:2016-11-25 00:00:00
abstract:BACKGROUND:Excessive blood loss in total joint arthroplasty (TJA) usually leads to an allogenic blood transfusion, which may cause adverse outcomes, prolonged length of hospitalization, and increased costs. The purpose of this study was to determine the incidence and risk factors for intraoperative and postoperative al...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1329-0
更新日期:2019-08-28 00:00:00
abstract::In the original publication of this article [1], Chinese text of the Figs. 3, 4, 5 has not been converted to English. ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,已发布勘误
doi:10.1186/s13018-020-01612-x
更新日期:2020-03-09 00:00:00
abstract:BACKGROUND:This study aimed to investigate the effects of silk fiber (SF)/calcium phosphate cement (CPC) biocomposite on repairing radial bone defects in rabbits. METHODS:Four-month-old New Zealand rabbits were selected to create a bilateral radial bone defect model and divided into four groups according to implanted ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-017-0529-8
更新日期:2017-02-21 00:00:00
abstract:BACKGROUND:Achilles sleeve avulsion usually occurs from pre-existing insertional Achilles tendinopathy, leaving a calcific spur at the insertional site. The purpose of this study was to introduce a new technique using the spur base on the insertional site to drill the suture tunnel to repair Achilles sleeve avulsion. ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-01699-2
更新日期:2020-06-17 00:00:00