Abstract：
BACKGROUND:Matrix metalloproteinase (MMPs) synthesized and secreted from connective tissue cells have been thought to participate in degradation of the extracellular matrix. Increased MMPs activities that degrade proteoglycans have been measured in osteoarthritis cartilage. This study aims to suppress the expression of the MMP-3 gene in in vitro human chondrosarcoma using siRNA. METHODS:CELLS WERE CATEGORIZED INTO FOUR GROUPS: control (G.1); transfection solution treated (G.2); negative control siRNA treated (G.3); and MMP-3 siRNA treated (G.4). All four groups were further subdivided into two groups - treated and non-treated with IL-1beta- following culture for 48 and 72 h. We observed the effects of gene suppression according to cell morphology, glycosaminoglycan (GAG) and hyaluronan (HA) production, and gene expression by using real-time polymerase chain reaction (PCR). RESULTS:In IL-1beta treated cells the apoptosis rate in G.4 was found to be lower than in all other groups, while viability and mitotic rate were higher than in all other groups (p < 0.05). The production of GAG and HA in G.4 was significantly higher than the control group (p < 0.05). MMP-3 gene expression was downregulated significantly (p < 0.05). CONCLUSION:MMP-3 specific siRNA can inhibit the expression of MMP-3 in chondrosarcoma. This suggests that MMP-3 siRNA has the potential to be a useful preventive and therapeutic agent for osteoarthritis.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Nganvongpanit K,Chaochird P,Siengdee P,Pothacharoen P,Klunklin K,Chomdej S,Mekchay S,Kongtaweelert Pdoi
10.1186/1749-799X-4-45subject
Has Abstractpub_date
2009-12-24 00:00:00pages
45issn
1749-799Xpii
1749-799X-4-45journal_volume
4pub_type
杂志文章abstract：BACKGROUND:Nursing intervention following discharge is a long-term rehabilitation process that is essential for improving hip joint function and quality of life in affected patients. This study aimed to assess the effect of nursing intervention via WeChat on the rehabilitation of patients after total hip arthroplasty (...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1483-4
更新日期：2019-12-09 00:00:00
abstract：BACKGROUND:Recently, the excellent outcomes of temporary fixation of C1-2 without fusion in the treatment of odontoid fracture had been reported. It is still unclear if this technique could achieve the equivalent outcomes as the golden standard technique of anterior screw fixation. The objective of this study is to com...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-017-0702-0
更新日期：2018-01-08 00:00:00
abstract：OBJECTIVE:The aim of this study is to investigate the morphological characteristics of distal radius die-punch fracture (DRDPF) with different types, based on the three-column theory. METHODS:The imaging data of 560 patients diagnosed with DRDPF were reviewed and divided into single-column, double-column, or three-col...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1453-x
更新日期：2019-11-27 00:00:00
abstract：BACKGROUND:This study aimed to explore the effect of the treatment through autologous fibula graft and hollow needle fixation to treat femoral head cutting after dynamic hip screw (DHS) fixation. METHODS:A total of 41 patients were admitted to the department of orthopedic trauma and received DHS fixation. Preoperative...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1521-2
更新日期：2020-01-15 00:00:00
abstract：BACKGROUND:Biological reconstruction surgery is a tough but alluring option for treating primary malignant musculoskeletal tumors. In this article, we evaluate the clinical outcomes of primary malignant musculoskeletal tumors treated with inactivated autograft using alcohol. METHOD:In this article, we include 58 patie...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0324-3
更新日期：2015-11-17 00:00:00
abstract：BACKGROUND:Ossification of the posterior longitudinal ligament (OPLL) of the spine is a complex, multifactorial disease. Although several genes that are linked to cervical OPLL susceptibility have been reported, specific genetic studies regarding thoracic OPLL are lacking. Whole-genome sequencing has been considered as...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-1022-8
更新日期：2018-12-22 00:00:00
abstract：BACKGROUND:Complications related to extensor tendons have begun to increase with the use of volar plates in the treatment of distal radius fractures. In this study, we aimed to compare four-plane radiography and ultrasonography in the evaluation of dorsal cortex screw penetration following volar plate fixation. METHOD...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0774-5
更新日期：2018-04-03 00:00:00
abstract：BACKGROUND:Flap transplantation is often needed for soft tissue defects of the fingers that cannot be closed directly. Sensory reconstruction of the fingers is important for patients to recover feelings. In this study, we report clinical outcomes of using free neurovascular transverse wrist crease flap for repairing fi...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,多中心研究
doi：10.1186/s13018-019-1444-y
更新日期：2019-11-14 00:00:00
abstract：BACKGROUND:Limited-incision total hip arthroplasty (THA) preserves hip abductors, posterior capsule, and external rotators potentially diminishing dislocation risk. However, potential complications also exist, such as component malposition. Specific implants have been manufactured that enhance compatibility with this t...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-016-0383-0
更新日期：2016-04-22 00:00:00
abstract：INTRODUCTION:The clinical outcome of hemiarthroplasty for proximal humeral fractures is not satisfactory. Secondary fragment dislocation may prevent bone integration; the primary stability by a fixation technique is therefore needed to accomplish tuberosity healing. Present technical comparison of surgical fixation tec...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章,评审
doi：10.1186/1749-799X-6-36
更新日期：2011-07-18 00:00:00
abstract：BACKGROUND:The anterior iliac crest (AIC) and proximal tibia (PT) are common donor sites for autologous bone graft harvesting. We compared pain levels at these harvest sites on 1 day, 5 days, 2 weeks, 4 weeks, and 8 weeks post-harvest. METHODS:We retrospectively reviewed 18 patients undergoing autologous bone grafting...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0820-3
更新日期：2018-05-16 00:00:00
abstract：BACKGROUND:Lubrication function is impaired and the lubricant content of synovial fluid (SF) changes immediately after plateau tibia fractures. Here, we aimed to analyze the lubricant content of SF at chronic term following plateau tibia fracture. METHODS:Forty-eight surgically treated patients without joint incongrue...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0232-6
更新日期：2015-06-03 00:00:00
abstract：BACKGROUND:Lengthening nails have been used to correct limb length discrepancy caused by different etiologies, as well as for post-traumatic reasons. Two important lengthening nail-related complications are damage to the distraction mechanism and femoral fractures around the nail tip. As a result of the curved anatomy ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-016-0345-6
更新日期：2016-01-16 00:00:00
abstract：BACKGROUND:Excessive blood loss in total joint arthroplasty (TJA) usually leads to an allogenic blood transfusion, which may cause adverse outcomes, prolonged length of hospitalization, and increased costs. The purpose of this study was to determine the incidence and risk factors for intraoperative and postoperative al...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1329-0
更新日期：2019-08-28 00:00:00
abstract：BACKGROUND:The major disadvantage of using a stem cell-based bone morphogenetic protein-4 (BMP4) gene therapy for skull defect is the overgrowth of generated bone tissue in situ. In the present study, to overcome bony overgrowth of stem cell based-gene therapy, a new strategy of two-stage bone tissue engineering by an ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0229-1
更新日期：2015-05-30 00:00:00
abstract：BACKGROUND:This study aimed to investigate the roles of lncRNA SNHG10 (SNHG10) and miR-218 in osteosarcoma (OS). METHODS:Paired OS and non-tumor tissues were collected from 58 OS patients. The expression of SNHG10 and miR-218 in tissue samples were determined by RT-qPCR. The interaction between SNHG10 and miR-218 was ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01865-6
更新日期：2020-08-26 00:00:00
abstract：BACKGROUND:Calcaneal insufficiency avulsion (CIA) fractures often present with neuropathic etiology, such as Charcot neuroarthropathy (CN). Under the same surgical procedures, the outcomes of CIA fractures are less desirable, compared to the outcomes of the traumatic calcaneal avulsion fractures. Here, the study sugges...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-017-0695-8
更新日期：2017-12-13 00:00:00
abstract：BACKGROUND:The onset of deep vein thrombosis (DVT) in patients after total hip arthroplasty (THA) may expand or enlarge and subsequently lead to significant mortality. The objective of this study was to investigate potential risk factors for DVT in patients after THA. METHODS:Eligible patients with hip joint diseases ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0716-2
更新日期：2018-01-10 00:00:00
abstract：BACKGROUND:The differences in the clinical and functional outcomes of closed reduction and percutaneous pin fixation and open reduction with internal fixation (ORIF) using plate and screws have been systematically synthesized by one meta-analysis. With newer studies being published, an effort to update the earlier meta...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-02057-y
更新日期：2020-11-19 00:00:00
abstract：BACKGROUND:Posterior tibial tendon (PTT) insufficiency is considered as the main cause of adult acquired flat foot and is three times more frequent in females. High estrogen levels exert a positive effect on the overall collagen synthesis in tendons. We have previously demonstrated the association between some genetic ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-1020-x
更新日期：2018-12-11 00:00:00
abstract：BACKGROUND:Obesity increases the risk for knee and hip joint implantation and negatively contributes to wound healing. In this study, in 52 patients undergoing hip and knee arthroplasty the amount of peripheral immune effector cells pre- and post-operative, as well as the expression of certain soluble factors affecting...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-017-0557-4
更新日期：2017-04-08 00:00:00
abstract：BACKGROUND:The aim of this research is to analyze the influence of Modic types on the clinical results of cervical spondylotic myelopathy treated by anterior cervical diskectomy and fusion. METHODS:A total of 106 patients with a mean age of 55.8 ± 6.5 years were included in this study. Patients with Modic changes were...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-014-0146-8
更新日期：2015-01-28 00:00:00
abstract：BACKGROUND:Disease modifying anti-rheumatic drugs (DMARDs) may interfere with bone healing. Previous studies give conflicting advice regarding discontinuation of these drugs in the peri-operative setting. No consensus exists in current practice especially with the newer DMARDs such as Leflunomide, Etanercept, and Infli...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-4-17
更新日期：2009-05-26 00:00:00
abstract：: The treatment of an above knee amputee who has sustained a fracture of femoral neck is a challenge for both the orthopaedic surgeon and the rehabilitation team. We present a case of such a patient and discuss different difficulties in his treatment. ...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-4-30
更新日期：2009-07-31 00:00:00
abstract：BACKGROUND:Ganglion cyst of cruciate ligaments is a rare lesion; the prevalence is 0.3-0.8%. The purpose of this study was to present clinical features of symptomatic posterior cruciate ligament (PCL) cyst, introduce the arthroscopic excision technique, and evaluate the clinical outcome. METHODS:A series of 11 patient...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0798-x
更新日期：2018-04-13 00:00:00
abstract：OBJECTIVES:The purpose of this study is to identify the factors that influence the length of stay (LOS) in total knee arthroplasty (TKA) patients with an enhanced recovery after surgery (ERAS) program. METHODS:Information from 167 patients (31 males and 136 females, range from 43 years to 88 years old) who underwent t...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1389-1
更新日期：2019-11-06 00:00:00
abstract：BACKGROUND:The aim of this study was to analyze the pattern and types of sensory nerve endings in ankle collateral ligaments using histological techniques, in order to observe the morphology and distribution of mechanoreceptors in the collateral ligaments of cadaver ankle joint, and to provide the morphological evidenc...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-015-0215-7
更新日期：2015-06-12 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-6-4
更新日期：2011-01-14 00:00:00
abstract：BACKGROUND:Graft shrinkage or radial extrusion is a reported complication after meniscus allograft transplantation (MAT). Whether shrinkage or extrusion progress after surgery and whether they are associated with the clinical outcome of MAT remain debatable. In this study, graft shrinkage and extrusion were measured in...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-018-0892-0
更新日期：2018-07-20 00:00:00
abstract：BACKGROUND:Until today, a reliable diagnostic discrimination between periprosthetic joint infections (PJI) and aseptic failure (AF) after total joint arthroplasty (TJA) remains challenging. Nearly all recent research focused on synovial markers to be elevated in PJI rather than in AF patients. In this study, synovial b...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-020-01718-2
更新日期：2020-05-27 00:00:00
