Abstract:
BACKGROUND:The major disadvantage of using a stem cell-based bone morphogenetic protein-4 (BMP4) gene therapy for skull defect is the overgrowth of generated bone tissue in situ. In the present study, to overcome bony overgrowth of stem cell based-gene therapy, a new strategy of two-stage bone tissue engineering by an adeno-associated virus containing BMP4 gene (AAV-BMP4) gene therapy was used. METHODS:AAV-BMP4 was purposely implanted in the skeletal muscle of mice to generate ectopic bone tissues during the first stage. Next, the newly formed ectopic bone tissues were harvested and then transplanted to repair the mouse skull defect during the second stage. RESULTS:The results showed that skeletal muscle implantation of AAV-BMP4 yielded a large amount of new bone tissues. The ectopic bone tissues can be harvested as a bone graft and can successfully repair the mouse skull defect without any bony overgrowth in situ. CONCLUSION:The results indicate that the bone tissues purposely generated by AAV-BMP4 in the skeletal muscle may be a new alternative of bone grafting for clinical purposes.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Tian K,Qi M,Wang L,Li Z,Xu J,Li Y,Liu G,Wang B,Huard J,Li Gdoi
10.1186/s13018-015-0229-1subject
Has Abstractpub_date
2015-05-30 00:00:00pages
86issn
1749-799Xpii
10.1186/s13018-015-0229-1journal_volume
10pub_type
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