Abstract:
BACKGROUND:Excessive inflammatory response under hyperglycemia can impair alveolar bone defect healing under diabetic conditions. NLRP3 (NACHT [nucleotide-binding oligomerization], LRR [leucine-rich repeat], and PYD [pyrin domain] domains-containing protein 3) inflammasome has been considered to play a crucial role in the inflammatory response, but its correlation with the impaired alveolar bone repair in diabetes still remains unclarified. The objective of the current study is to investigate the effect of NLRP3 inflammasome inhibition by a lentiviral short hairpin RNA (shRNA) targeting NLRP3 on alveolar bone defect healing in diabetic rats. METHODS:Diabetes was induced in rats by high-fat diet and streptozotocin injection, and alveolar bone defects in both maxillae were created by surgery. Then, the lentiviral shRNA targeting NLRP3 was applied in the defect. Eight weeks after surgery, the alveolar bone regeneration was examined using hematoxylin and eosin (H&E) staining, and the gene expression in the bone healing site was detected using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis and western blot analysis. RESULTS:H&E staining showed that treatment with lentiviral shRNA targeting NLRP3 could increase the bone regeneration score in the alveolar bone defect of diabetic rats. Additionally, qRT-PCR analysis and western blot analysis of the bone defect demonstrated that this shRNA inhibited the expression of NLRP3, apoptosis-associated speck-like protein containing a CARD, caspase-1, and proinflammatory cytokine interleukin-1β and increased the expression of osteogenic markers Runt-related transcription factor 2 and osteocalcin. CONCLUSIONS:Our findings suggested that inhibition of NLRP3 inflammasome could improve alveolar bone defect healing in diabetic rats. The beneficial effect may correlate with reduced proinflammatory cytokine production and increased osteogenic gene expression in hyperglycemia.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Li H,Zhong X,Chen Z,Li Wdoi
10.1186/s13018-019-1215-9subject
Has Abstractpub_date
2019-05-30 00:00:00pages
167issue
1issn
1749-799Xpii
10.1186/s13018-019-1215-9journal_volume
14pub_type
杂志文章abstract:BACKGROUND:Dynamic spine implants were developed to prevent adjacent segment degeneration (ASD) and adjacent segment disease (ASDi). Purpose of this study was to investigate the clinical and radiological outcomes of "topping off" devices following lumbar spinal fusion procedure using a PEEK-based dynamic rod system. Mo...
journal_title:Journal of orthopaedic surgery and research
pub_type: 临床试验,杂志文章
doi:10.1186/s13018-018-0905-z
更新日期:2018-08-09 00:00:00
abstract:BACKGROUND:This study aimed to explore the relationship among different cervical sagittal parameters in asymptomatic volunteers and the correlation between surgical efficacy and difference of presumed and actual postoperative C2-7 Cobbs's angle (C2-7COBB), which was calculated based on preoperative T1 slope (T1S) in pa...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
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更新日期:2020-11-05 00:00:00
abstract:BACKGROUND:In lumbar spinal stenosis (LSS), at most times, several levels are impaired and selecting the correct level remains a common problem for surgeons, as surgery remains invasive, and extended laminectomy may lead to secondary surgical complications. Therefore, helping to select the correct level may be useful f...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1065-5
更新日期:2019-01-22 00:00:00
abstract::Acute traumatic patellar dislocation is a common injury in the active and young adult populations. MRI of the knee is recommended in all patients who present with acute patellar dislocation. Numerous operative and non-operative methods have been described to treat the injuries; however, the ideal management of the acu...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,评审
doi:10.1186/1749-799X-7-21
更新日期:2012-06-06 00:00:00
abstract:BACKGROUND:The shape of the patella has been considered to be a predisposing factor resulting in patellar instability, but the effects of abnormal patella position during its development are unclear. The present study evaluated patellar morphological changes after patella instability and evaluated the influence of pate...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-017-0615-y
更新日期:2017-07-11 00:00:00
abstract:INTRODUCTION:The aim of this study was to determine the differences and consistencies in the morphological and angular interpretations of standard USG images. Therefore, it was aimed to show the correlations of orthopaedic doctors with different periods of experience in hip ultrasound measurements taken with the Graf m...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1263-1
更新日期:2019-07-17 00:00:00
abstract:BACKGROUND:Hyperleptinemia is a common feature of obese people, and leptin, an adipocyte-derived cytokine, is believed to be an important factor in the pathogenesis of cervical ossification of the posterior longitudinal ligament(C-OPLL). So this research was to identify the relation between the serum leptin and bone me...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-0864-4
更新日期:2018-07-03 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1130-0
更新日期:2019-03-20 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-6-62
更新日期:2011-12-21 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-01699-2
更新日期:2020-06-17 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-015-0175-y
更新日期:2015-03-25 00:00:00
abstract:BACKGROUND:Entrapment of the superior cluneal nerve (SCN) in an osteofibrous tunnel has been reported as a cause of low back pain (LBP). However, there are few reports on the prevalence of SCN disorder and there are several reports only on favorable outcomes of treatment of SCN disorder on LBP. The purposes of this pro...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-014-0139-7
更新日期:2014-12-31 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-0774-5
更新日期:2018-04-03 00:00:00
abstract:BACKGROUND:Sternoclavicular joint (SCJ) infectious arthritis is a rare disease. A standard treatment for SCJ infection has not been established. This study aimed to assess the clinical outcomes of a three-stage procedure with bone transportation (BT) for treating SCJ infectious arthritis. METHODS:Six patients (mean ag...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0480-0
更新日期:2016-11-25 00:00:00
abstract:BACKGROUND:We performed a systematic review and meta-analysis of randomized controlled trials (RCTs) to evaluate the efficacy and safety of local infiltration anesthesia (LIA) versus epidural analgesia (EPA) for postoperative pain control in total knee arthroplasty (TKA). METHODS:In December 2017, a systematic compute...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s13018-018-0770-9
更新日期:2018-05-16 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1432-2
更新日期:2019-11-14 00:00:00
abstract:BACKGROUND:Although medical intervention of periprosthetic bone loss in the immediate postoperative period was recommended, not all the patients experienced periprosthetic bone loss after total hip arthroplasty (THA). Prediction tools that enrolled all potential risk factors to calculate an individualized prediction of...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-02034-5
更新日期:2020-11-02 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1198-6
更新日期:2019-06-07 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-014-0057-8
更新日期:2014-07-17 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-3-28
更新日期:2008-07-16 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 临床试验,杂志文章
doi:10.1186/s13018-016-0472-0
更新日期:2016-11-14 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1440-2
更新日期:2019-11-28 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-021-02234-7
更新日期:2021-02-01 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-1020-x
更新日期:2018-12-11 00:00:00
abstract:OBJECTIVE:To discuss the sensitivity and specificity of the combinations of multiple factors that work on bone infection after artificial joint, and provide evidence-based medical basis for the early diagnosis of infection after artificial joint. METHODS:A retrospective review was conducted on 35 patients diagnosed wi...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-01669-8
更新日期:2020-04-25 00:00:00
abstract:PURPOSE:The aim of this retrospective observational study of one cohort was to evaluate the long-term outcome in patients younger than 60 years after total hip arthroplasty using a straight uncemented stem and an uncemented threaded cup. METHODS:Between 1986 and 1987, 75 hips of 75 patients (mean age, 53.35 ± 6.17 yea...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-02102-w
更新日期:2020-11-26 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
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更新日期:2018-03-27 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-8-8
更新日期:2013-04-11 00:00:00
abstract:BACKGROUND:The treatment of articular cartilage damage is a major clinical problem. More often, this clinical issue affects children, which forces doctors to find the best treatment method. METHODS:The aim of this experimental study on 2-month-old Landrace pigs was to compare the results of two cartilage defect treatm...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1302-y
更新日期:2019-08-15 00:00:00
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journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
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更新日期:2020-12-11 00:00:00