Abstract：
BACKGROUND:Plasmodium ovale is widely distributed across tropical regions and has two closely related but distinct species, namely P. ovale curtisi and P. ovale wallikeri. Combining genetic characterization with the immunogenicity of merozoite surface protein-8 (MSP-8) supports considering MSP-8 as a candidate target for blood-stage vaccines against malaria. However, no previous studies have focused on characterizing the genetic diversity and immunogenicity of PoMSP-8. METHODS:Blood samples were collected from 42 patients infected with P. ovale. The patients were migrant workers returning to the Jiangsu Province from Africa; genomic DNA was extracted from their blood samples for sequencing and protein expression. The recombinant PoMSP-8 (rPoMSP-8) proteins were expressed and purified to assess their immune responses in BALB/c mice. RESULTS:The sequences of the P. ovale curtisi and P. ovale wallikeri msp8 genes were completely conserved in each isolate. The rPoMSP-8 proteins were successfully expressed and purified as ~70 kDa proteins. Antibodies raised against rPoMSP-8 in mice showed appropriate immunoreactivity, as evidenced by immunoblotting. These specific antibodies were detected at day 7 post-immunization, and their levels increased throughout the whole immunization period. rPoMSP-8-raised antibodies had high endpoint titers (1:5,120,000) and high avidity (PocMSP-8: 94.84%, PowMSP-8: 92.69%). Cross-reactivity between rPocMSP-8 and rPowMSP-8 was observed with each anti-PoMSP8-specific antibody. CONCLUSIONS:Remarkable conservation and high immunogenicity was observed in both rPoMSP-8s. Intriguingly, cross-reaction between rPocMSP-8 and rPowMSP-8 was detected, suggesting that a single PoMSP8-based construction might be applicable for both species.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Zhang X,Chu R,Xu S,Fu H,Tang J,Chen L,Shi X,Chen J,Li Y,Zhu G,Han ET,Xuan Y,Cao J,Cheng Ydoi
10.1186/s13071-019-3412-0subject
Has Abstractpub_date
2019-04-11 00:00:00pages
164issue
1issn
1756-3305pii
10.1186/s13071-019-3412-0journal_volume
12pub_type
杂志文章abstract：BACKGROUND:Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory. Thus far, only few attempts have been made...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3493-9
更新日期：2019-05-17 00:00:00
abstract：BACKGROUND:The efficacy of fluralaner for the treatment of Otodectes cynotis infestations in dogs and cats was evaluated after oral (dogs) or topical administration (dogs and cats). Twenty-four dogs and sixteen cats were experimentally infested with O. cynotis and randomly allocated to equal sized groups (n = 8/group)....
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1954-y
更新日期：2017-01-16 00:00:00
abstract：BACKGROUND:This laboratory study compared the efficacy of Bravecto™ (fluralaner), formulated as a chewable tablet, with the efficacy of Advocate® (imidacloprid/moxidectin), formulated for topical administration, against naturally acquired generalized demodicosis in dogs. METHODS:Sixteen dogs, all diagnosed with genera...
journal_title：Parasites & vectors
pub_type： 杂志文章,随机对照试验
doi：10.1186/s13071-015-0775-8
更新日期：2015-03-28 00:00:00
abstract：BACKGROUND:Chagas disease, caused by the parasite Trypanosoma cruzi, is mainly transmitted by blood-sucking bugs called triatomines. In the Yucatán Peninsula, Mexico, the main vector of T. cruzi is Triatoma dimidiata. While this species may colonize houses in other regions, it is mostly intrusive in Yucatán: it general...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1852-3
更新日期：2016-11-03 00:00:00
abstract：BACKGROUND:Canine vector-borne diseases (CVBDs) are caused by a wide range of pathogens transmitted to dogs by arthropods including ticks and insects. Many CVBD-agents are of zoonotic concern, with dogs potentially serving as reservoirs and sentinels for human infections. The present study aimed at assessing the seropr...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-5-62
更新日期：2012-03-27 00:00:00
abstract：BACKGROUND:Intestinal parasite infections (IPIs) are among the most significant causes of illness and disease of socially and economically disadvantaged populations in developing countries, including rural areas of the People's Republic of China. With the spread of the human immunodeficiency virus (HIV) among rural Chi...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-5-36
更新日期：2012-02-13 00:00:00
abstract：BACKGROUND:Bovine besnoitiosis, caused by the apicomplexan parasite Besnoitia besnoiti, is a chronic and debilitating cattle disease that notably impairs fertility. Acutely infected bulls may develop respiratory signs and orchitis, and sterility has been reported in chronic infections. However, the pathogenesis of acut...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-3959-9
更新日期：2020-03-02 00:00:00
abstract：BACKGROUND:Hemotropic mycoplasmas are epicellular erythrocytic bacteria that can cause infectious anemia in some mammalian species. Worldwide, hemotropic mycoplasmas are emerging or re-emerging zoonotic pathogens potentially causing serious and significant health problems in wildlife. The objective of this study was to...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-7-117
更新日期：2014-03-24 00:00:00
abstract：BACKGROUND:Reliable maps of the geographical distribution, number of infected individuals and burden estimates of schistosomiasis are essential tools to plan, monitor and evaluate control programmes. Large-scale disease mapping and prediction efforts rely on compiled historical survey data obtained from the peer-review...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-4-142
更新日期：2011-07-20 00:00:00
abstract：BACKGROUND:Spatial modelling studies of schistosomiasis (SCH) are now commonplace. Covariate values are commonly extracted at survey locations, where infection does not always take place, resulting in an unknown positional exposure mismatch. The present research aims to: (i) describe the nature of the positional exposu...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-3039-6
更新日期：2018-08-13 00:00:00
abstract：BACKGROUND:Plasmodium falciparum delayed clearance with the use of artemisinin-based combination therapy (ACTs) has been reported in some African countries. Single nucleotide polymorphisms (SNPs) in two genes, P. falciparum adaptor protein complex 2 mu subunit (pfap2mu) and ubiquitin specific protease 1 (pfubp1), have ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2762-3
更新日期：2018-03-12 00:00:00
abstract：BACKGROUND:Malaria vector control in Africa relies on insecticides targeting adult mosquito vectors via insecticide treated nets or indoor residual spraying. Despite the proven efficacy of these strategies, the emergence and rapid rise in insecticide resistance in malaria vectors raises many concerns about their sustai...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1354-3
更新日期：2016-02-05 00:00:00
abstract：BACKGROUND:Trypanosomosis is a haemoprotozoan disease, mostly transmitted by the tsetse fly (Glossina spp.), it causes severe disease in humans and animals in Sub-Saharan Africa (SSA). The disease results in loss of livestock and agricultural productivity with severe socio-economic impacts. In Ethiopia, bovine trypanos...
journal_title：Parasites & vectors
pub_type： 杂志文章,meta分析
doi：10.1186/s13071-016-1404-x
更新日期：2016-03-10 00:00:00
abstract：:Schistosomiasis is a major disease of public health importance in humans occurring in 76 countries of the tropics and sub-tropics. In China, schistosomiasis japonica is one of the highest priorities in communicable disease control defined by the central government. Since 1970s, the habitats of Biomphalaria straminea, ...
journal_title：Parasites & vectors
pub_type： 杂志文章,评审
doi：10.1186/1756-3305-6-249
更新日期：2013-08-28 00:00:00
abstract：BACKGROUND:Lymphatic filariasis (LF) is a debilitating disease associated with extensive disfigurement and is one of a diverse group of diseases referred to as neglected tropical diseases (NTDs) which mainly occur among the poorest populations. In line with global recommendations to eliminate LF, Kenya launched its LF ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2044-5
更新日期：2017-02-22 00:00:00
abstract：BACKGROUND:Antigen B (EgAgB) is an abundant lipoprotein released by the larva of the cestode Echinococcus granulosus into the host tissues. Its protein moiety belongs to the cestode-specific family known as hydrophobic ligand binding protein (HLBP), and is encoded by five gene subfamilies (EgAgB8/1-EgAgB8/5). The funct...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1350-7
更新日期：2016-02-04 00:00:00
abstract：BACKGROUND:Different species of apicomplexan protozoans of the genera Hepatozoon and Cytauxzoon can infect domestic cats, but their epidemiology and clinical relevance are not fully understood. The aim of this study was to assess the molecular prevalence of Hepatozoon spp. and Cytauxzoon spp. and to identify associated...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2056-1
更新日期：2017-03-13 00:00:00
abstract：BACKGROUND:African trypanosomosis is a disease of public health and economic importance that poses a major threat to the livelihoods of people living in the Maasai Steppe, where there is a significant interaction between people, livestock and wildlife. The vulnerability of the Maasai people to the disease is enhanced b...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-017-2411-2
更新日期：2017-10-23 00:00:00
abstract：BACKGROUND:Red foxes (Vulpes vulpes) are one of the most widespread wild carnivores in the world, being recognized to harbor and transmit a wide range of vector-borne diseases. Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato are zoonotic tick-borne pathogens causing emerging diseases. Wild animals play an...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-015-1130-9
更新日期：2015-10-08 00:00:00
abstract：BACKGROUND:Thelazia callipaeda is a zoonotic nematode that affects the eyes of domestic and wild animals, including dogs, cats and red foxes. This parasitic eye worm is transmitted by Phortica variegata, which is a zoophilic fruit fly spread in Europe. Two wild European rabbits (Oryctolagus cuniculus) found dead in nor...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1526-1
更新日期：2016-05-10 00:00:00
abstract：BACKGROUND:The ability of blood-feeding arthropods to successfully acquire and transmit pathogens of medical and veterinary importance has been shown to be interfered with, or enhanced by, the arthropod's native microbiome. Mosquitoes transmit viruses, protozoan and filarial nematodes, the majority of which contribute ...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-020-04218-8
更新日期：2020-07-13 00:00:00
abstract：BACKGROUND:The Cqm1 α-glucosidase of Culex quinquefasciatus larvae acts as the midgut receptor for the binary toxin of the biolarvicide Lysinibacillus sphaericus. Mutations within the cqm1 gene can code for aberrant polypeptides that can no longer be properly expressed or bind to the toxin, leading to insect resistance...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1347-2
更新日期：2016-02-04 00:00:00
abstract：BACKGROUND:Loop-mediated isothermal amplification (LAMP) is a very specific, efficient, and rapid gene amplification procedure in which the reaction can run at a constant temperature. In the current study we have developed a LAMP assay to improve the diagnosis of Fasciola spp. in the faeces of sheep. FINDINGS:After th...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1355-2
更新日期：2016-02-05 00:00:00
abstract：BACKGROUND:Cats appear to be the primary reservoir host for Bartonella koehlerae, an alpha Proteobacteria that is most likely transmitted among cat populations by fleas (Ctenocephalides felis). Bartonella koehlerae has caused endocarditis in a dog and in one human patient from Israel, but other clinically relevant repo...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-3-76
更新日期：2010-08-24 00:00:00
abstract：BACKGROUND:Tick cell lines are now available from fifteen ixodid and argasid species of medical and veterinary importance. However, some tick cell lines can be difficult to cryopreserve, and improved protocols for short- and long-term low temperature storage will greatly enhance their use as tools in tick and tick-born...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-3-37
更新日期：2010-04-13 00:00:00
abstract：BACKGROUND:In Lithuania, the first case of canine subcutaneous dirofilariosis was recorded in 2010. Since then, an increasing number of cases of canine dirofilariosis have been documented in different veterinary clinics throughout the country. Human dirofilariosis was diagnosed in Lithuania for the first time in Septem...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-019-3406-y
更新日期：2019-04-18 00:00:00
abstract：BACKGROUND:Northeastern Italy is a hotspot for several tick-borne pathogens, transmitted to animals and humans mainly by Ixodes ricinus. Here we compare the results of molecular monitoring of ticks and zoonotic TBPs over a six-year period, with the monitoring of red foxes (Vulpes vulpes) in an endemic area. RESULTS:In...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-018-2726-7
更新日期：2018-03-20 00:00:00
abstract：BACKGROUND:Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-016-1344-5
更新日期：2016-02-02 00:00:00
abstract：BACKGROUND:The South-to-North Water Diversion Project (SNWDP) is the largest national water conservancy project in China. However, the Eastern Route Project (ERP) of SNWDP will refer to the habitats of Oncomelania hupensis, the intermediate host of Schistosoma japonicum. The present study was aimed at investigating the...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/1756-3305-5-52
更新日期：2012-03-20 00:00:00
abstract：BACKGROUND:Polovodova method based on counting follicular dilatations estimates the number of egg-laying in mosquitoes. However, some researchers doubt the reliability of this method because of the absence of multiple dilatations in vectors after many gonotrophic cycles. It is in this context of controversy that our st...
journal_title：Parasites & vectors
pub_type： 杂志文章
doi：10.1186/s13071-015-0731-7
更新日期：2015-02-22 00:00:00
