Abstract:
:Cancerous invasion yields unusual metabolisms providing a significant amount of peptide albuminomes that modulate albumin stability via binding. The study aimed at the investigation of the thermal stability of human plasma albumin with breast cancer of various stages by means of differential scanning calorimetry (DSC). Blood plasma was drawn from 11 female breast cancer patients and 50 healthy volunteers of homogeneous demographics. Plasma samples and their albumin-enriched fractions were subjected to DSC scanning between 37 and 90°C at 5°C/min rate. For normal blood plasma, a characteristic signature of DSC tracing was observed. And, the deconvolution of DSC thermograms revealed the recognition of thermal transition of albumin. It was found that denaturing temperature of albumin increased with increasing breast cancer staging which implied the increase in albuminome/peptide abundance produced by cancerous invasion. The analysis of albumin denaturing energetics based on rational approximation of the simple Lumry-Eyring model demonstrated that thermal transition of free albumin and albuminome-bound form attained energetic levels expressed as apparent activation energy (Eapp ± s.e.) of 132.68 ± 14.21 and 46.76 ± 8.42 Kcal/mol corresponded to the schemes dominated by irreversible alteration and by reversible unfolding, respectively. Thus, Eapp value may indicate the degrees of cancerous invasion. It was proposed that Eapp may be used as an indicator to diagnose and assess the prognosis of breast cancer.
journal_name
AAPS PharmSciTechjournal_title
AAPS PharmSciTechauthors
Faroongsarng D,Sunpaweravong S,Raksawong Adoi
10.1208/s12249-019-1356-5subject
Has Abstractpub_date
2019-03-18 00:00:00pages
146issue
4issn
1530-9932pii
10.1208/s12249-019-1356-5journal_volume
20pub_type
杂志文章abstract::The purpose of this research was to develop mouth dissolve tablets of nimesulide. Granules containing nimesulide, camphor, crospovidone, and lactose were prepared by wet granulation technique. Camphor was sublimed from the dried granules by exposure to vacuum. The porous granules were then compressed. Alternatively, t...
journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章,随机对照试验
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journal_title:AAPS PharmSciTech
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/s12249-017-0710-8
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/s12249-020-1618-2
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/s12249-017-0825-y
更新日期:2018-01-01 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/pt020423
更新日期:2001-11-19 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/pt060352
更新日期:2005-10-22 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/s12249-010-9464-2
更新日期:2010-09-01 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/s12249-019-1577-7
更新日期:2019-12-26 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/s12249-020-01712-1
更新日期:2020-07-07 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/s12249-019-1382-3
更新日期:2019-07-01 00:00:00
abstract::The solubility of five poorly soluble drugs was enhanced by using an effervescence assisted solid dispersion (EASD) technique. EASDs were prepared by using modified fusion method. Drug and hydrophilic carrier were melted, and in this molten mixture, effervescence was generated by adding effervescence couple comprising...
journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/s12249-015-0381-2
更新日期:2015-12-01 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
doi:10.1208/s12249-018-0998-z
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
abstract::The aim of the present study was to investigate the use of different grades of microcrystalline cellulose (MCC) and lactose in a direct pelletization process in a rotary processor. For this purpose, a mixed 2- and 3-level factorial study was performed to determine the influence of the particle size of microcrystalline...
journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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更新日期:2005-10-24 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:AAPS PharmSciTech
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更新日期:2006-03-24 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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更新日期:2016-08-01 00:00:00
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journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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abstract::Ambient air humidity and temperature are known to influence the mechanical strength of tablets. The objective of this work is to understand the influence of processing parameters and environmental conditions (humidity and temperature) on the strength of bilayer tablets. As part of this study, bilayer tablets were comp...
journal_title:AAPS PharmSciTech
pub_type: 杂志文章
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journal_title:AAPS PharmSciTech
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