Abstract:
BACKGROUND:Proinflammatory cytokines, which can upregulate the expression of matrix-degrading enzymes in chondrocytes, play important roles in the development of osteoarthritis. BET family proteins, acting as the "readers" of acetylated modifications on histones, have been linked to transcriptional regulation. And a BET protein inhibitor, I-BET151, has been shown to inhibit the induction of matrix-degrading enzymes by proinflammatory cytokines in chondrocytes. Our objective is to clarify the role and mechanism of BET proteins on matrix-degrading enzyme gene expression by using a human chondrosarcoma cell line (SW1353). METHODS:We pretreated SW1353 cells with I-BET151 prior to treatment with IL-1β or TNF-α and then checked the expression of four matrix-degrading enzyme genes (MMP1, MMP3, MMP13, and ADAMTS4). We performed knockdown of BET protein family members (BRD2, BRD3, and BRD4) with corresponding siRNAs in SW1353 cells prior to treatment with IL-1β or TNF-α and checked the expression of the matrix-degrading enzyme genes. We evaluated Brd-mediated transcriptional regulation on the matrix-degrading enzyme genes by ChIP assay. RESULTS:We confirmed that I-BET151 could suppress the IL-1β- or TNF-α-induced expression of MMP1, MMP3, MMP13, and ADAMTS4 in SW1353 cells. Brd3 and Brd4 were required for the IL-1β- or TNF-α-induced expression of matrix-degrading enzyme genes in SW1353 cells. We revealed that inducible acetylation of H4k5/8/12 and the recruitment of Brd3, Brd4, and p-TEFb to chromatin were involved in IL-1β- or TNF-α-induced transcription. CONCLUSIONS:Our findings suggested that Brd3 and Brd4 were essential for the IL-1β- or TNF-α-induced transcription of matrix-degrading enzyme genes, and recruitment of Brd3 and Brd4 to chromatin of these genes played the main role in this process.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Dai J,Zhou S,Ge Q,Qin J,Li J,Ju H,Cao Y,Zheng M,Li C,Gao X,Teng H,Jiang Qdoi
10.1186/s13018-019-1091-3subject
Has Abstractpub_date
2019-02-20 00:00:00pages
59issue
1issn
1749-799Xpii
10.1186/s13018-019-1091-3journal_volume
14pub_type
杂志文章abstract:BACKGROUND:Individualized and accurate implantation of a femoral component during total knee arthroplasty (TKA) is essential in achieving equal distribution of intra-articular stress and long-term survival of the prosthesis. However, individualized component implantation remains challenging. This study aimed to optimiz...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-0891-1
更新日期:2018-07-20 00:00:00
abstract:BACKGROUND:The transoral atlantoaxial reduction plate (TARP) is an effective advance in the treatment of atlantoaxial dislocation (AAD) and can enable the performance of anterior atlantoaxial release, reduction, decompression, and internal fixation in a one-stage operation. However, accurate transoral C2 pedicle insert...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,随机对照试验
doi:10.1186/s13018-019-1189-7
更新日期:2019-05-16 00:00:00
abstract:BACKGROUND:This meta-analysis aims to illustrate the efficacy and safety of intrathecal morphine (ITM) versus femoral nerve block (FNB) for pain control after total knee arthroplasty (TKA). METHODS:In April 2017, a systematic computer-based search was conducted in PubMed, EMBASE, Web of Science, Cochrane Database of S...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,meta分析
doi:10.1186/s13018-017-0621-0
更新日期:2017-08-16 00:00:00
abstract:BACKGROUND:The relationship between collar design of a femoral component and iliopsoas impingement (IPI) after total hip arthroplasty (THA) is still underrecognized. The purpose of our study was to determine the possible risk factors for IPI related to the femoral component, when using a collared femoral prosthesis. M...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-01787-3
更新日期:2020-07-16 00:00:00
abstract:BACKGROUND:Intraarticular scar adhesion refers to a serious complication caused by knee surgery or trauma, leading to various sequelae (e.g., articular cartilage degeneration and knee joint stiffness). Artesunate (ART) has exhibited an effect to suppress fibroblast proliferation, whereas the exact mechanism remains unc...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1445-x
更新日期:2019-12-17 00:00:00
abstract:BACKGROUND:To date, there has been no adequate biomechanical model that would allow a quantitative comparison in terms of stability/stiffness between a corpectomy with the posterior column preserved and a total spondylectomy with the posterior column sacrificed. The objective of this study was to perform a biomechanica...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-015-0240-6
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:Sternoclavicular joint (SCJ) infectious arthritis is a rare disease. A standard treatment for SCJ infection has not been established. This study aimed to assess the clinical outcomes of a three-stage procedure with bone transportation (BT) for treating SCJ infectious arthritis. METHODS:Six patients (mean ag...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0480-0
更新日期:2016-11-25 00:00:00
abstract:BACKGROUND:The treatment of articular cartilage damage is a major clinical problem. More often, this clinical issue affects children, which forces doctors to find the best treatment method. METHODS:The aim of this experimental study on 2-month-old Landrace pigs was to compare the results of two cartilage defect treatm...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1302-y
更新日期:2019-08-15 00:00:00
abstract:BACKGROUND:Kashin-Beck disease (KBD) is an endemic and chronic osteoarthropathy. At present, the diagnosis of KBD mainly depends on the X-ray examination and which could not reflect early damage of cartilage sensitively. So, the aim of this study was to find effective and sensitive biomarkers for early diagnosis of ped...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,多中心研究
doi:10.1186/s13018-018-1057-x
更新日期:2019-01-11 00:00:00
abstract:BACGROUND:Although the contribution of fibroblast growth factor (FGF)-2 and mesenchymal stromal cells (MSCs) to bone formation is well known, few studies have investigated the combination of an autologous bone graft with FGF-2 or MSCs for large bone defects. METHODS:We studied an atrophic non-union model with a large ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0442-6
更新日期:2016-09-26 00:00:00
abstract:BACKGROUND:Posterior tibial tendon (PTT) insufficiency is considered as the main cause of adult acquired flat foot and is three times more frequent in females. High estrogen levels exert a positive effect on the overall collagen synthesis in tendons. We have previously demonstrated the association between some genetic ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-1020-x
更新日期:2018-12-11 00:00:00
abstract:BACKGROUND:Periprosthetic joint infection (PJI) is a devastating complication following total joint arthroplasty (TJA). Now, the definition of PJI traditionally used in clinical practice was set out by the International Consensus Meeting (ICM) and Infectious Diseases Society (IDSA). There was a new definition proposed ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1185-y
更新日期:2019-05-24 00:00:00
abstract:BACKGROUND:The aim of the current paper is to evaluate the effects of robot-navigation-assisted core decompression compared with conventional core decompression surgery for early-stage osteonecrosis of the femoral head. METHODS:Twenty patients with a total of 36 hips who were diagnosed with Association Research Circul...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1437-x
更新日期:2019-11-21 00:00:00
abstract:BACKGROUND:Percutaneous fixation with cannulated screws is an effective method for treating medial malleolar epiphyseal fractures, which comprise a portion of bimalleolar and trimalleolar fractures. Lateral malleolar fractures also need to be fixed to achieve anatomical reduction and absolute stable fixation of the ank...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1287-6
更新日期:2019-08-08 00:00:00
abstract:BACKGROUND:Femoral and sciatic nerves could be damaged during various stages of the periacetabular osteotomy. Changing the position of the hip could be the most effective way of preventing nerve injuries. The purpose of the present study was to investigate the distances of the nerves to various bony landmarks with diff...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-01937-7
更新日期:2020-09-11 00:00:00
abstract:BACKGROUND:When performing arthroscopic ankle arthrodesis for end-stage ankle arthritis, internal fixation is performed using bone screws after appropriate preparation. However, optimal characteristics of bone screws have not been examined in terms of pressure force. Objective comparisons of bone-screw performance may ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0503-x
更新日期:2017-01-17 00:00:00
abstract:PURPOSE:The purpose of this study was to compare the interfragmentary compression force across a simulated scaphoid fracture by two commonly used compression screw systems; the Acutrak 2 Standard and the 3.0 mm Synthes headless compression screw. METHODS:Sixteen (8 pairs; 6 female, 2 male) cadaver scaphoids were rando...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-6-27
更新日期:2011-06-07 00:00:00
abstract:BACKGROUND:Complications related to extensor tendons have begun to increase with the use of volar plates in the treatment of distal radius fractures. In this study, we aimed to compare four-plane radiography and ultrasonography in the evaluation of dorsal cortex screw penetration following volar plate fixation. METHOD...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-0774-5
更新日期:2018-04-03 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate the efficacy of local infiltration anaesthesia (LIA) during primary total hip arthroplasty (THA) via a posterolateral approach under general anaesthesia and to compare the efficacy of LIA in all layers with LIA in the deep and superficial fascia. PATIENTS AND METHODS:On...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-02182-8
更新日期:2021-01-22 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells (MSCs) have great potential for the repair and regeneration of bone fracture, but their optimal origins remain controversial. METHODS:Bone marrow-MSCs (BM-MSCs) and bone-bone marrow-MSCs (B-BM-MSCs) were isolated from 12 SD rats, and the morphology, MSC-associated markers, and prolife...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1346-z
更新日期:2019-09-03 00:00:00
abstract::Submuscular and minimally invasive plate insertion is gaining popularity reducing the need for large open approaches and resulting in a smaller operative 'footprint.' With pediatric fractures and titanium implants, fibrous and osseous ingrowth to the implant and osseous implant integration may interfere with implant r...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-8-21
更新日期:2013-07-11 00:00:00
abstract:BACKGROUND:Elbow arthroscopy had good functional outcome for throwing athletes. Returning to sports is a major concern for all athletes, but only a few reports have investigated the clinical factors related to the duration of returning to sports. The present study evaluates the efficacy of elbow arthroscopic surgery on...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-0992-x
更新日期:2018-11-07 00:00:00
abstract:BACKGROUND:This study aims to compare clinical outcomes of distraction arthroplasty alone versus combined with arthroscopic microfracture in treating post-traumatic ankle arthritis. METHODS:The study cohort consisted of 96 patients (96 ankles) who underwent distraction arthroplasty alone or combined with arthroscopic ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-017-0546-7
更新日期:2017-03-17 00:00:00
abstract:BACKGROUND:With the patient in a constant supine position, elevation of the femur in THA (DAA) provides a more intuitive and conducive location of the acetabulum for the correct placement of the acetabular prosthesis, but elevation of the femur for broaching becomes more challenging. The purpose of this study is to ana...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-1012-x
更新日期:2018-12-04 00:00:00
abstract:BACKGROUND:Attempts have been made to validate the significance of remnant preservation with anterior cruciate ligament (ACL) reconstruction using immunohistochemical and immunocytochemical techniques. The purpose of this study was to examine the expression of mechanoreceptors in the remnant tissue of ACL reconstructio...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-017-0593-0
更新日期:2017-06-14 00:00:00
abstract:PURPOSE:To compare the specificity and sensitivity of preoperative D-dimer and age-adjusted D-dimer value for predicting the incidence of the DVT preoperatively in total joint arthroplasty (TJA) patients. METHODS:We enrolled 406 patients finally above 50 years old. Everyone had done ultrasonography bedside, and D-dime...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-02172-w
更新日期:2021-01-25 00:00:00
abstract:BACKGROUND:Intervertebral disk degeneration (IDD) is a degenerative disease characterized by cytoplasm loss and extracellular matrix degradation. Numerous evidence reported that miRNAs participated in IDD development. Nevertheless, the function of miR-142-3p in IDD development remains unknown. This study mainly explore...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-02194-4
更新日期:2021-01-14 00:00:00
abstract:BACKGROUND:Activities that require extreme hip movement can dislocate hip implants in the early post operative phase. One such activity is retrieving an object from the floor. The aim of this study was to assess hip movement using four different techniques to accomplish this task. This assessment would identify the tec...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-6-11
更新日期:2011-02-21 00:00:00
abstract:OBJECTIVE:The objective of this study was to evaluate the incidence of radiographic osteoarthritis in the operated knee in comparison with the contralateral knee ten years after a bone-tendon bone patellar autograft ACL-reconstruction and to evaluate to which level patients regain activity ten years after reconstructio...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-3-24
更新日期:2008-06-10 00:00:00
abstract:BACKGROUND:To investigate the efficacy and safety of endoscopic transnasal anterior release and posterior reduction without odontoidectomy to treat irreducible atlantoaxial dislocation (IAAD). METHODS:A series of 9 patients with IAAD underwent endoscopic transnasal anterior release and posterior reduction without odon...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1167-0
更新日期:2019-05-06 00:00:00