Abstract：
BACKGROUND:Quercetin (QCT) is a flavonol present in many vegetables, it is proved to show chemo preventive effect against lung, cervical, prostate, breast and colon cancer due to its anti-inflammatory, anti-tumor and anti-oxidant property. Looking into the reported chemo-preventive effect we speculated antitumor activity in retinoblastoma (RB) Y79 cells, we also studied the molecular mechanism for antitumor activity. METHODS:The effect of QCT on Y79 cell viability count was done by cell counting kit, cell cycle distribution, apoptosis studies and mitochondrial membrane potential was evaluated by flow cytometry. Protein expression was done by western blot analysis. RESULTS:The outcomes of study showed that QCT reduced Y79 cell viability and caused arrest of G1 phase in cell cycle via decreasing the expression levels of cyclin-dependent kinase (CDK)2/6 and cyclin D3 and by increasing the levels of both CDK inhibitor proteins p21 and p27. Apoptosis of Y79 cells mediated by QCT occurred via activation of both caspases-3/-9. Flow cytometry studies showed that QCT caused collapse in mitochondrial membrane potential (ΔΨm) in Y79 cells. Western blot studies confirmed that QCT brought about phosphorylation of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK). We also established that inhibitors of JNK and p38 MAPK suppressed QCT mediated activation of both caspases-3/-9 and subdued the apoptosis of cancerous Y79 cells. CONCLUSION:All the results of the study suggest that QCT induced the apoptosis of Y79 cells via activation of JNK and p38 MAPK pathways, providing a novel treatment approach for human RB.
journal_name
BMC Complement Altern Medjournal_title
BMC complementary and alternative medicineauthors
Liu H,Zhou Mdoi
10.1186/s12906-017-2023-6subject
Has Abstractpub_date
2017-12-13 00:00:00pages
531issue
1issn
1472-6882pii
10.1186/s12906-017-2023-6journal_volume
17pub_type
杂志文章abstract：BACKGROUND:Free radical stress leads to tissue injury and can eventually to arthritis, atherosclerosis, diabetes mellitus, neurodegenerative diseases and carcinogenesis. Several studies are ongoing worldwide to find natural antioxidants of plant origin. We assessed the in-vitro antioxidant activities and screened the p...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-11-69
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journal_title：BMC complementary and alternative medicine
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journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-128
更新日期：2012-08-17 00:00:00
abstract：BACKGROUND:Acupuncture is an effective complement to pharmacological therapy in the alleviation of chronic pelvic inflammatory disease (PID). It has mild or no side effects; however, a minimum of 3 months of therapy is required to guarantee a beneficial outcome. This study investigates why patients insist on acupunctur...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-14-345
更新日期：2014-09-21 00:00:00
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journal_title：BMC complementary and alternative medicine
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doi：10.1186/s12906-018-2176-y
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abstract：BACKGROUND:To evaluate and compare effects of an integrative treatment (IT), therapeutic acupuncture (TA), and conventional treatment (CT) in alleviating symptoms of anxiety and depression in psychologically distressed primary care patients. METHODS:An open, pragmatic randomized controlled trial comparing the three tr...
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pub_type： 杂志文章,随机对照试验
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更新日期：2013-11-07 00:00:00
abstract：BACKGROUND:Mineral based preparations are widely used for centuries as antimicrobial agents. However, the efficacy and the mode of action of mineral based preparations are uncertain due to the insufficient antimicrobial studies. Arogyawardhana Vati (AV) and Manikya Rasa (MR) are such two Rasashastra herbo-minerallic dr...
journal_title：BMC complementary and alternative medicine
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doi：10.1186/s12906-016-1336-1
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abstract：BACKGROUND:Nausea and vomiting during pregnancy (NVP) affects two-thirds of pregnant women to varying degrees and over the years many modalities have been used to try to alleviate this often debilitating condition. There is a paucity of information in the literature about the use or efficacy of complementary and altern...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-2-5
更新日期：2002-05-17 00:00:00
abstract：BACKGROUND:Throughout the world, patients with chronic diseases/illnesses use complementary and alternative medicines (CAM). The use of CAM is also substantial among patients with diseases/illnesses of unknown aetiology. Chronic fatigue syndrome (CFS), also termed myalgic encephalomyelitis (ME), is no exception. Hence,...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,评审
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更新日期：2011-10-07 00:00:00
abstract：BACKGROUND:Rhodiola rosea (Rhodiola) is a plant in the Crassulaceae family that grows in cold regions of the world. It is mainly used in clinics as an adaptogen. Recently, it has been mentioned that Rhodiola increases plasma β-endorphin to lower blood pressure. Thus, the present study aims to investigate the antidiabet...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-14-20
更新日期：2014-01-13 00:00:00
abstract：BACKGROUND:Arctium lappa L. (Asteraceae), burdock, is a medicinal plant that is popularly used for treating hypertension, gout, hepatitis, and other inflammatory disorders. This study was performed to test the effect of ethanol extract of Arctium lappa L. (EAL) seeds on vascular reactivity and inflammatory factors in r...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-116
更新日期：2012-08-06 00:00:00
abstract：BACKGROUND:On account of deterioration of chronic heart failure (CHF) and extensive exploration of Chinese herbal injections (CHIs), we performed a network meta-analysis to investigate the efficacy of CHIs (Huangqi injection, Shenfu injection, Shengmai injection, Shenmai injection, Shenqi Fuzheng injection, Yiqifumai i...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,meta分析
doi：10.1186/s12906-018-2090-3
更新日期：2018-01-31 00:00:00
abstract：BACKGROUND:Plant medicinal extracts may be claimed to prevent or cure chemical intoxications. Few of these are tested for their mechanisms of actions in vivo and for their cellular impacts. In 2011, we demonstrated that hepatic cell mortality induced by environmentally realistic levels of the widely used herbicide Roun...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-016-1226-6
更新日期：2016-07-22 00:00:00
abstract：BACKGROUND:Although electroacupuncture (EA) relieves various types of pain, individual differences in the sensitivity to EA analgesia have been reported, causing experimental and clinical difficulties. Our functional genomic study using cDNA microarray identified that 5'-AMP-activated protein kinase (AMPK), a well-know...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-14-211
更新日期：2014-06-30 00:00:00
abstract：BACKGROUND:The liver plays an essential role in the body by regulating several important metabolic functions. Liver injury is associated with the distortion of these functions causing many health problems. Pharmaceutical drugs treat liver disorders but cause further damage to it. Hence, herbal drugs are used worldwide ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-13-160
更新日期：2013-07-05 00:00:00
abstract：BACKGROUND:Threatened miscarriage involves vaginal bleeding in a pregnancy that remains viable. This is a common early pregnancy complication with increased risk factors for early pregnancy loss, preterm premature rupture of membranes (PPROM), preterm delivery, low birth weight babies and maternal antepartum haemorrhag...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-20
更新日期：2012-03-22 00:00:00
abstract：BACKGROUND:Whole-body electrical myostimulation (WB-EMS) is a relatively recent training methodology that has been extraordinarily used in recent years. However, there is a lack of consensus on the effectiveness of WB-EMS in the situations in which its use has been largely popularized. The objective of this systematic ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,meta分析
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更新日期：2019-04-24 00:00:00
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pub_type： 杂志文章,随机对照试验
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更新日期：2014-07-01 00:00:00
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journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-13-93
更新日期：2013-05-01 00:00:00
abstract：BACKGROUND:The use of herbal medicines as complements or alternatives to orthodox medicines has been on the increase. There has been the erroneous belief that these medicines are free from adverse effects. Schefflera barteri is popularly used in the West region of Cameroon for the treatment of various diseases such as ...
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doi：10.1186/s12906-015-0581-z
更新日期：2015-03-07 00:00:00
abstract：BACKGROUND:Fibrosis is a major contributor to systemic sclerosis (SSc)-related morbidity, and rapid, progressive skin involvement predicts later mortality. Western medicine therapies for SSc cannot produce satisfactory effects currently, while Traditional Chinese Medicine (TCM), such as the Wenyang Huazhuo Tongluo (WYH...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-016-1056-6
更新日期：2016-02-20 00:00:00
abstract：BACKGROUND:Liver steatosis was caused by lipid accumulation in the liver. Alisma orientale (AO) is recognized as a promising candidate with therapeutic efficacy for the treatment of nonalcoholic fatty liver disease (NAFLD). HepG2 hepatocyte cell line is commonly used for liver disease cell model. METHOD:The HepG2 cell...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
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更新日期：2016-07-25 00:00:00
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journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12906-018-2101-4
更新日期：2018-01-31 00:00:00
abstract：BACKGROUND:Launaea procumbens (Asteraceae) is used as a folk medicine to treat hepatic disorders in Pakistan. The effect of a chloroform extract of Launaea procumbens (LPCE) was evaluated against carbon-tetrachloride (CCl4)-induced liver damage in rats. METHODS:To evaluate the hepatoprotective effects of LPCE, 36 male...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-114
更新日期：2012-08-03 00:00:00
abstract：BACKGROUND:Ucche (Momordica charantia L. var. muricata (Willd.) Chakravarty) has been reported to possess many benefits and medicinal properties. However, the protective effect of ucche against carbon tetrachloride (CCl4) induced hepatotoxicity have not been clarified fully yet. The aim of the present study was to inve...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-015-0636-1
更新日期：2015-04-11 00:00:00
abstract：BACKGROUND:Aim of the present study was to evaluate anti-inflammatory activity of newly developed polyherbal formulations DF1911, DF2112 and DF2813. These newly developed formulations are modifications of Dashamoola, a well known Ayurvedic formulation, along with addition of new plants. METHODS:Complete Freund's adjuv...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-017-1711-6
更新日期：2017-04-04 00:00:00
abstract：BACKGROUND:The use of dietary supplements (DS) is common among persons with dementia. Direct risks associated with DS use include adverse events and DS-drug interactions. A direct risk is a risk caused by the treatment itself. Indirect risks are related to the treatment setting, such as the conditions of use, and not t...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-017-1765-5
更新日期：2017-05-12 00:00:00
abstract：BACKGROUND:Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry. METHODS:The present study was conducted to evaluate the in vitro anticancer and apoptotic effects on cel...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-015-0836-8
更新日期：2015-09-02 00:00:00
abstract：BACKGROUND:Records have shown that Combretum adenogonium Steud. Ex A. Rich (Combretaceae) is used in traditional medicine systems of several tribes in Tanzania. This study focused on the investigation of antibacterial activity, anti-HIV-1 protease activity, toxicity properties and classes of phytochemicals in extracts ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-163
更新日期：2012-09-26 00:00:00
