Gene expression profiling reveals underlying molecular mechanism of hepatoprotective effect of Phyllanthus niruri on thioacetamide-induced hepatotoxicity in Sprague Dawley rats.

Abstract:

BACKGROUND:The liver plays an essential role in the body by regulating several important metabolic functions. Liver injury is associated with the distortion of these functions causing many health problems. Pharmaceutical drugs treat liver disorders but cause further damage to it. Hence, herbal drugs are used worldwide and are becoming increasingly popular. METHODS:The hepatoprotective activity of Phyllanthus niruri (PN) was evaluated against liver cirrhosis induced by thioacetamide (TAA) in male Sprague Dawley rats. Rats received intraperitoneal injections of thioacetamide (TAA, 200 mg/kg, b.w. three times weekly) for eight weeks. Daily treatments with plant extract (200 mg/kg) were administered orally for eight weeks. At the end of the study, hepatic damage was evaluated by monitoring transforming growth factor (TGFβ), collagen α1 (Collα1), matrix metalloproteinase-2 (MMP2) and tissue inhibitor of matrix metalloproteinase-1 (TIMP1) gene expression by real-time PCR. Moreover, different chromatographic techniques including column chromatography, thin layer chromatography, and Ultra Performance Liquid Chromatography (UPLC) with Liquid Chromatography/Mass Spectrometry (LC/MS) were used to isolate the active constituents of the plant. RESULTS:The results revealed that treatment with PN significantly reduced the effect of thioacetamide toxicity and exhibited effective hepatoprotective activity. The mechanism of the hepatoprotective effect of PN is proposed to be by normalizing ROSs. Additionally, PN treatment regulated the expression of TGFβ, Collα1, MMP2, and TIMP1 genes. In the active fraction of P. niruri, the isolated chemical constituents were 4-O-caffeoylquinic acid and quercetin 3-O-rhamnoside. CONCLUSIONS:The results of the present study indicate that PN ethanol extracts possess hepatoprotective activity that is most likely because of the isolated chemical constituents.

authors

Amin ZA,Alshawsh MA,Kassim M,Ali HM,Abdulla MA

doi

10.1186/1472-6882-13-160

subject

Has Abstract

pub_date

2013-07-05 00:00:00

pages

160

issn

1472-6882

pii

1472-6882-13-160

journal_volume

13

pub_type

杂志文章
  • What do clinicians want? Interest in integrative health services at a North Carolina academic medical center.

    abstract:BACKGROUND:Use of complementary medicine is common, consumer driven and usually outpatient focused. We wished to determine interest among the medical staff at a North Carolina academic medical center in integrating diverse therapies and services into comprehensive care. METHODS:We conducted a cross sectional on-line s...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-7-5

    authors: Kemper KJ,Dirkse D,Eadie D,Pennington M

    更新日期:2007-02-09 00:00:00

  • Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae).

    abstract:BACKGROUND:Endophytes, which reside in plant tissues, have the potential to produce novel metabolites with immense benefits for health industry. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell were investigated. METHODS:Endophytic fungi were isolated from the Bacop...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-14-52

    authors: Katoch M,Singh G,Sharma S,Gupta N,Sangwan PL,Saxena AK

    更新日期:2014-02-11 00:00:00

  • Role of ellagic acid in regulation of apoptosis by modulating novel and atypical PKC in lymphoma bearing mice.

    abstract:BACKGROUND:Protein kinase C regulates various cellular processes including cell proliferation, cell adhesion, apoptosis, angiogenesis, invasion, and metastasis. Activation of different PKC isozymes results in distinct cellular responses. Novel PKCs are mainly involved in apoptotic process. Atypical PKC subfamily plays ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0810-5

    authors: Mishra S,Vinayak M

    更新日期:2015-08-15 00:00:00

  • Protective effect of Danggui (Radix Angelicae Sinensis) on angiotensin II-induced apoptosis in H9c2 cardiomyoblast cells.

    abstract:BACKGROUND:Danggui (Radix Angelicae Sinensis) is an herb often used in Traditional Chinese medicine. It is used to promote blood flow and has been used in the treatment of myocardial ischemia-reperfusion injury in animal models. Angiotensin II (Ang II) has been shown to play important roles in mediating cardiovascular ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-14-358

    authors: Huang CY,Kuo WW,Kuo CH,Tsai FJ,Liu PY,Hsieh DJ

    更新日期:2014-09-25 00:00:00

  • An in vitro study on the risk of non-allergic type-I like hypersensitivity to Momordica charantia.

    abstract:BACKGROUND:Momordica charantia (MC) is a tropical plant that is extensively used in folk medicine. However, the knowledge about side effects of this plant is relatively little according to knowledge about its therapeutic effects. The aim of this study is to reveal the effects of non-allergic type-I like hypersensitivit...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-284

    authors: Sagkan RI

    更新日期:2013-10-26 00:00:00

  • The combination of Artemisia princeps Pamp, Leonurus japonicas Houtt, and Gardenia jasminoides Ellis fruit attenuates the exacerbation of energy, lipid, and glucose by increasing hepatic PGC-1α expression in estrogen-deficient rats.

    abstract:BACKGROUND:Artemisia princeps Pamp (APP), Leonurus japonicas Houtt (LJH), and Gardenia jasminoides Ellis fruit (GJE) have been traditionally used in East Asia to treat women's diseases related to reproductive system. They may attenuate the deterioration of energy, lipid, glucose and bone metabolism by estrogen deficien...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1109-x

    authors: Yang HJ,Kim MJ,Kwon DY,Moon BR,Kim AR,Kang S,Park S

    更新日期:2016-05-23 00:00:00

  • Effects of Payena dasyphylla (Miq.) on hyaluronidase enzyme activity and metalloproteinases protein expressions in interleukin-1β stimulated human chondrocytes cells.

    abstract:BACKGROUND:Hyaluronidases have been found as the target enzymes in the development of osteoarthritis (OA) disease. While there is still no curative treatment for this disease, recent studies on the treatment of OA were focused on the effectiveness of natural products which are expected to improve the symptoms with mini...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-213

    authors: Citalingam K,Zareen S,Shaari K,Ahmad S

    更新日期:2013-08-23 00:00:00

  • Psychophysical responses in patients receiving a mock laser within context of an acupuncture clinical trial: an interoceptive perspective.

    abstract:BACKGROUND:The psychophysical responses induced by verum acupuncture are characterized by a constellation of unique subjective sensory responses commonly termed De Qi. Furthermore, a variety of sham interventions have been used as a control for acupuncture clinical trials. Indeed, one such control has been mock laser w...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1186/s12906-017-1859-0

    authors: Razavy S,Gadau M,Zhang SP,Wang FC,Bangrazi S,Berle C,Harahap M,Li T,Li WH,Zaslawski C

    更新日期:2017-07-03 00:00:00

  • Additional effects of acupuncture on early comprehensive rehabilitation in patients with mild to moderate acute ischemic stroke: a multicenter randomized controlled trial.

    abstract:BACKGROUND:Acupuncture is not considered a conventional therapy for post-stroke sequelae but it might have some additional positive effects on early rehabilitation. We conducted this trial to determine whether acupuncture has additional effects in early comprehensive rehabilitation for acute ischemic stroke and dysfunc...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1186/s12906-016-1193-y

    authors: Chen L,Fang J,Ma R,Gu X,Chen L,Li J,Xu S

    更新日期:2016-07-18 00:00:00

  • Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice.

    abstract:BACKGROUND:Syzygium campanulatum Korth (Myrtaceae) is an evergreen shrub rich in phenolics, flavonoid antioxidants, and betulinic acid. This study sought to investigate antiangiogenic and anti-colon cancer effects of S.C. standardized methanolic extract. METHODS:Betulinic acid was isolated from methanolic extract by c...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-168

    authors: Aisha AF,Ismail Z,Abu-Salah KM,Siddiqui JM,Ghafar G,Abdul Majid AM

    更新日期:2013-07-11 00:00:00

  • Antioxidant, anti-adipocyte differentiation, antitumor activity and anthelmintic activities against Anisakis simplex and Hymenolepis nana of yakuchinone A from Alpinia oxyphylla.

    abstract:BACKGROUND:Alpinia oxyphylla is a common remedy in traditional Chinese medicine. Yakuchinone A is a major constituent of A. oxyphylla and exhibits anti-inflammatory, antitumor, antibacterial, and gastric protective activities. METHODS:Antioxidant and antitumor characteristics of yakuchinone A in skin cancer cells as w...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-237

    authors: Lin RJ,Yen CM,Chou TH,Chiang FY,Wang GH,Tseng YP,Wang L,Huang TW,Wang HC,Chan LP,Ding HY,Liang CH

    更新日期:2013-09-26 00:00:00

  • Enzyme inhibitory and antioxidant activities of traditional medicinal plants: potential application in the management of hyperglycemia.

    abstract:BACKGROUND:Traditional Indian and Australian medicinal plant extracts were investigated to determine their therapeutic potential to inhibit key enzymes in carbohydrate metabolism, which has relevance to the management of hyperglycemia and type 2 diabetes. The antioxidant activities were also assessed. METHODS:The eval...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-12-77

    authors: Gulati V,Harding IH,Palombo EA

    更新日期:2012-06-19 00:00:00

  • Urox containing concentrated extracts of Crataeva nurvala stem bark, Equisetum arvense stem and Lindera aggregata root, in the treatment of symptoms of overactive bladder and urinary incontinence: a phase 2, randomised, double-blind placebo controlled tri

    abstract:BACKGROUND:Storage lower urinary tract symptoms (LUTS) including overactive bladder (OAB) and urinary incontinence (UI) affect millions of people worldwide, significantly impacting quality of life. Plant based medicines have been documented both empirically and in emerging scientific research to have varying benefits i...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/s12906-018-2101-4

    authors: Schoendorfer N,Sharp N,Seipel T,Schauss AG,Ahuja KDK

    更新日期:2018-01-31 00:00:00

  • Antileptospiral activity of xanthones from Garcinia mangostana and synergy of gamma-mangostin with penicillin G.

    abstract:BACKGROUND:Leptospirosis, one of the most widespread zoonotic infectious diseases worldwide, is caused by spirochetes bacteria of the genus Leptospira. The present study examined inhibitory activity of purified xanthones and crude extracts from Garcinia mangostana against both non-pathogenic and pathogenic leptospira. ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-182

    authors: Seesom W,Jaratrungtawee A,Suksamrarn S,Mekseepralard C,Ratananukul P,Sukhumsirichart W

    更新日期:2013-07-19 00:00:00

  • Perspectives on the use of aromatherapy from clinicians attending an integrative medicine continuing education event.

    abstract:BACKGROUND:The use of essential oils is growing in the United States, but clinician attitudes, experience, and beliefs regarding their use have not previously been studied. METHODS:One hundred five of 106 clinician attendees (99.1%) of an integrative medicine continuing education conference were surveyed using an audi...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-019-2572-y

    authors: Pearson ACS,Cutshall SM,Hooten WM,Rodgers NJ,Bauer BA,Bhagra A

    更新日期:2019-07-12 00:00:00

  • Antibacterial activities of the methanol extracts of ten Cameroonian vegetables against Gram-negative multidrug-resistant bacteria.

    abstract:BACKGROUND:Many edible plants are used in Cameroon since ancient time to control microbial infections. This study was designed at evaluating the antibacterial activities of the methanol extracts of ten Cameroonian vegetables against a panel of twenty nine Gram negative bacteria including multi-drug resistant (MDR) stra...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-26

    authors: Noumedem JA,Mihasan M,Lacmata ST,Stefan M,Kuiate JR,Kuete V

    更新日期:2013-01-31 00:00:00

  • Perceptions of traditional Chinese medicine for chronic disease care and prevention: a cross-sectional study of Chinese hospital-based health care professionals.

    abstract:BACKGROUND:In China, demands for disease prevention and health care and the prevalence of chronic non-communicable diseases have increased. TCM and general hospitals are increasingly utilizing TCM strategies for chronic non-communicable disease care and prevention. This study aimed to investigate health care profession...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-018-2273-y

    authors: Fan X,Meng F,Wang D,Guo Q,Ji Z,Yang L,Ogihara A

    更新日期:2018-07-06 00:00:00

  • Medicinal and ethnoveterinary remedies of hunters in Trinidad.

    abstract:BACKGROUND:Ethnomedicines are used by hunters for themselves and their hunting dogs in Trinidad. Plants are used for snakebites, scorpion stings, for injuries and mange of dogs and to facilitate hunting success. RESULTS:Plants used include Piper hispidum, Pithecelobium unguis-cati, Bauhinia excisa, Bauhinia cumanensis...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-1-10

    authors: Lans C,Harper T,Georges K,Bridgewater E

    更新日期:2001-01-01 00:00:00

  • Effectiveness Guidance Document (EGD) for acupuncture research - a consensus document for conducting trials.

    abstract:BACKGROUND:There is a need for more Comparative Effectiveness Research (CER) to strengthen the evidence base for clinical and policy decision-making. Effectiveness Guidance Documents (EGD) are targeted to clinical researchers. The aim of this EGD is to provide specific recommendations for the design of prospective acup...

    journal_title:BMC complementary and alternative medicine

    pub_type: 共识发展会议,杂志文章

    doi:10.1186/1472-6882-12-148

    authors: Witt CM,Aickin M,Baca T,Cherkin D,Haan MN,Hammerschlag R,Hao JJ,Kaplan GA,Lao L,McKay T,Pierce B,Riley D,Ritenbaugh C,Thorpe K,Tunis S,Weissberg J,Berman BM,Effectiveness Guidance Documents Collaborators.

    更新日期:2012-09-06 00:00:00

  • Factors associated with herbal use among urban multiethnic primary care patients: a cross-sectional survey.

    abstract:BACKGROUND:The use of herbal supplements in the United States has become increasingly popular. The prevalence of herbal use among primary care patients varies in previous studies; the pattern of herbal use among urban racially/ethnically diverse primary care patients has not been widely studied. The primary objectives ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-4-18

    authors: Kuo GM,Hawley ST,Weiss LT,Balkrishnan R,Volk RJ

    更新日期:2004-12-02 00:00:00

  • Cyanidin-3-rutinoside acts as a natural inhibitor of intestinal lipid digestion and absorption.

    abstract:BACKGROUND:Cyanidin-3-rutinoside (C3R), a naturally occurring anthocyanin, possesses anti-oxidant, anti-hyperglycemic, anti-glycation and cardioprotective properties. However, its mechanisms responsible for anti-hyperlipidemic activity have not been fully identified. The aim of the study was to investigate the lipid-lo...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-019-2664-8

    authors: Thilavech T,Adisakwattana S

    更新日期:2019-09-05 00:00:00

  • A six-month crossover chemoprevention clinical trial of tea in smokers and non-smokers: methodological issues in a feasibility study.

    abstract:BACKGROUND:Chemoprevention crossover trials of tea can be more efficient than parallel designs but the attrition and compliance rates with such trials are unknown. METHODS:Attrition (dropouts) and compliance with treatment were assessed in a 25-week randomized, placebo controlled, crossover, feasibility clinical trial...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/1472-6882-12-96

    authors: Dash C,Chung FL,Rohan JA,Greenspan E,Christopher PD,Makambi K,Hara Y,Newkirk K,Davidson B,Adams-Campbell LL

    更新日期:2012-07-16 00:00:00

  • Effects of Ginkgo biloba on chemically-induced mammary tumors in rats receiving tamoxifen.

    abstract:BACKGROUND:Ginkgo biloba extract (GbE) is used extensively by breast cancer patients undergoing treatment with Tamoxifen (TAM). Thus, the present study investigated the effects of GbE in female Sprague-Dawley (SD) rats bearing chemically-induced mammary tumors and receiving TAM. METHODS:Animals bearing mammary tumors ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-93

    authors: Dias MC,Furtado KS,Rodrigues MA,Barbisan LF

    更新日期:2013-05-01 00:00:00

  • Cytotoxicity and sub-acute toxicity in Crl:CD (SD) rats of traditional herbal formula Ojeok-san.

    abstract:BACKGROUND:Although Ojeok-san (OJS), an oriental herbal formula, has been used in Asian countries including Korea, China and Japan to treat the common cold and illnesses including fatigue and gastrointestinal disorders, there is little information of its safety and toxicity in vivo and in vitro. METHODS:In the present...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0582-y

    authors: Jeong SJ,Huh JI,Shin HK

    更新日期:2015-03-01 00:00:00

  • Decisions to use complementary and alternative medicine (CAM) by male cancer patients: information-seeking roles and types of evidence used.

    abstract:BACKGROUND:Complementary and Alternative Medicine (CAM) is increasingly popular with cancer patients and yet information provision or discussion about CAM by health professionals remains low. Previous research suggests that patients may fear clinicians' 'disapproval' if they raise the subject of CAM, and turn to other ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-7-25

    authors: Evans M,Shaw A,Thompson EA,Falk S,Turton P,Thompson T,Sharp D

    更新日期:2007-08-04 00:00:00

  • Fungal vincristine from Eutypella spp - CrP14 isolated from Catharanthus roseus induces apoptosis in human squamous carcinoma cell line -A431.

    abstract:BACKGROUND:Catharanthus roseus, a medicinal plant, is known to produce secondary metabolites, vincristine and vinblastine, which are terpenoid indole alkaloids. Previously we have reported that Eutypella spp - CrP14 isolated from stem cutting of this plant had shown significant antiproliferative activity when tested in...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1299-2

    authors: Kuriakose GC,Palem PP,Jayabaskaran C

    更新日期:2016-08-22 00:00:00

  • Dichrostachys cinerea (L.) Wight et Arn (Mimosaceae) hydro-alcoholic extract action on the contractility of tracheal smooth muscle isolated from guinea-pig.

    abstract:BACKGROUND:Dichrostachys cinerea (L.) Wight et Arn. (Mimosaceae) is largely used in ethno-medically across Africa, and mainly employed for the treatment of asthma in Ivory Coast and Gabon. The paper analyses the relaxation induced by the methanolic extract of D. cinerea (Edici) in the guinea-pig trachea preparations (G...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-11-23

    authors: Aworet-Samseny RR,Souza A,Kpahé F,Konaté K,Datté JY

    更新日期:2011-03-17 00:00:00

  • Adding Chinese herbal medicine to conventional therapy brings cognitive benefits to patients with Alzheimer's disease: a retrospective analysis.

    abstract:BACKGROUND:Conventional therapy (CT) such as donepezil and memantine are well-known short-term treatments for the symptoms of Alzheimer's disease (AD). The efficacy of them, however, drops below baseline level after 9 months. In China, herbal therapy as a complementary therapy is very popular. Should conventional thera...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-017-2040-5

    authors: Shi J,Ni J,Lu T,Zhang X,Wei M,Li T,Liu W,Wang Y,Shi Y,Tian J

    更新日期:2017-12-13 00:00:00

  • Current challenges and future directions for naturopathic medicine in Australia: a qualitative examination of perceptions and experiences from grassroots practice.

    abstract:BACKGROUND:Naturopaths are an increasingly significant part of the healthcare sector in Australia, yet despite their significant role there has been little research on this practitioner group. Currently the naturopathic profession in Australia is undergoing a period of rapid professional growth and change. However, to ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-15

    authors: Wardle JL,Adams J,Lui CW,Steel AE

    更新日期:2013-01-14 00:00:00

  • An evaluation of the completeness of safety reporting in reports of complementary and alternative medicine trials.

    abstract:BACKGROUND:Adequate reporting of safety in publications of randomized controlled trials (RCTs) is a pre-requisite for accurate and comprehensive profile evaluation of conventional as well as complementary and alternative medicine (CAM) treatments. Clear and concise information on the definition, frequency, and severity...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-11-67

    authors: Turner LA,Singh K,Garritty C,Tsertsvadze A,Manheimer E,Wieland LS,Galipeau J,Moher D

    更新日期:2011-08-22 00:00:00