Abstract：
BACKGROUND:Catharanthus roseus, a medicinal plant, is known to produce secondary metabolites, vincristine and vinblastine, which are terpenoid indole alkaloids. Previously we have reported that Eutypella spp - CrP14 isolated from stem cutting of this plant had shown significant antiproliferative activity when tested in vitro against HeLa cell line. The present study was conducted to identify the anticancer compound responsible for the anti-proliferative activity of the fungal extract and to evaluate its in vitro anticancer and apoptotic effects. METHODS:The anti-proliferative activity of the fungal anticancer compound, vincristine was analyzed by MTT assay against different cancer cell lines. We examined its efficacy of apoptotic induction on A431 cells. The parameters examined included cell cycle distribution, loss of mitochondrial membrane potential (MMP), DNA fragmentation and reactive oxygen species (ROS) generation. RESULTS:The presence of vincristine in fungal culture filtrate was confirmed through chromatographic and spectroscopic analyses, and the amount was estimated to be 53 ± 5.0 μg/l. The partially purified fungal vincristine had strong cytotoxic activity towards human squamous carcinoma cells - A431 in the MTT assay. Furthermore, we showed that the fungal vincristine was capable of inducing apoptosis in A431 cells through generation of reactive oxygen species and activation of the intrinsic pathway leading to loss of MMP. CONCLUSIONS:We have demonstrated for the first time that the vincristine from Eutypella spp - CrP14 is an efficient inducer of apoptosis in A431 cells, meriting its further evaluation in vivo.
journal_name
BMC Complement Altern Medjournal_title
BMC complementary and alternative medicineauthors
Kuriakose GC,Palem PP,Jayabaskaran Cdoi
10.1186/s12906-016-1299-2subject
Has Abstractpub_date
2016-08-22 00:00:00pages
302issue
1issn
1472-6882pii
10.1186/s12906-016-1299-2journal_volume
16pub_type
杂志文章abstract：BACKGROUND:Abalone has long been used as a valuable food source in East Asian countries. Although the nutritional importance of abalone has been reported through in vitro and in vivo studies, there is little evidence about the potential anti-tumor effects of abalone visceral extract. The aim of the present study is to ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-10-60
更新日期：2010-10-20 00:00:00
abstract：BACKGROUND:Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effec...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-13-19
更新日期：2013-01-22 00:00:00
abstract：BACKGROUND:Previous studies have revealed that gypenosides (GPS) improve the symptoms of anxiety disorders in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned rat model of Parkinson's disease (PD). The present study aimed to investigate the effects of GPS on memory deficits in an MPTP-lesioned mouse model...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-017-1959-x
更新日期：2017-09-06 00:00:00
abstract：BACKGROUND:The use of edible plants is an integral part of dietary behavior in the West region of Cameroon. Dorstenia psilurus (Moraceae) is widely used as spice and as medicinal plant for the treatment of several diseases in Cameroon. The aim of this study is to investigate the cytotoxic and apoptotic potential of met...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-13-223
更新日期：2013-09-10 00:00:00
abstract：BACKGROUND:In the U.S., complementary and alternative medicine (CAM) use is most prevalent among reproductive age, educated women. We sought to determine general attitudes and approaches to CAM among obstetric and gynecology patients and physicians. METHODS:Obstetrician-gynecologist members of the American Medical Ass...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-8-35
更新日期：2008-06-26 00:00:00
abstract：BACKGROUND:Lysophosphatidylcholine (lysoPC), a metabolite from membrane phospholipids, accumulates in the ischemic myocardium and plays an important role in the development of myocardial dysfunction ventricular arrhythmia. In this study, we investigated if baicalein, a major component of Huang Qui, can protect against ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-14-233
更新日期：2014-07-09 00:00:00
abstract：BACKGROUND:Verbascum thapsus is used in tribal medicine as an antispasmodic, anti-tubercular agent and wormicide. In this study, we investigated the antispasmodic and anthelmintic activities of crude aqueous methanolic extract of the plant. METHODS:V. thapsus extracts were tested against roundworms (Ascaridia galli) a...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-29
更新日期：2012-03-30 00:00:00
abstract：BACKGROUND:Uncomplicated chronic rachialgia is a highly prevalent complaint, and one for which therapeutic results are contradictory. The aim of the present study is to evaluate the effectiveness and safety of treatment with auriculopressure, in the primary healthcare sector, carried out by trained healthcare professio...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1186/1472-6882-8-36
更新日期：2008-07-06 00:00:00
abstract：:The editor has retracted this article [1] due to overlap with a previously published article [2]. Both articles also appear to use the same image representing different fungal mycelial growths. S.K. Sharma and N.S. Atri do not agree to this retraction. N. Gautam has not responded to correspondence from the editor abou...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,撤回出版物
doi：10.1186/s12906-018-2344-0
更新日期：2018-10-16 00:00:00
abstract：BACKGROUND:Ficus benghalensis L. (Banyan) is a commonly found tree in Eastern Nepal. Its different plant parts are used for various neurological ailments. This study was performed in mice to see its effects in various neuropharmacological parameters. METHODS:Passive-avoidance (memory), Open-field (anxiety), Pentobarbi...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-016-1413-5
更新日期：2016-11-03 00:00:00
abstract：BACKGROUND:The purpose of this study was to compare the efficacy of electroacupuncture (EA), diclofenac and their combination in symptomatic treatment of osteoarthritis (OA) of the knee. METHODS:This study was a randomized, single-blind, placebo controlled trial. The 193 out-patients with OA of the knee were randomize...
journal_title：BMC complementary and alternative medicine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1186/1472-6882-2-3
更新日期：2002-03-21 00:00:00
abstract：BACKGROUND:Periodontitis has been implicated as a risk factor for metabolic disorders associated with insulin resistance. Recently, we have demonstrated that orally administered Porphyromonas gingivalis, a representative periodontopathic bacterium, induces endotoxemia via reduced gut barrier function coupled with chang...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-016-1305-8
更新日期：2016-08-30 00:00:00
abstract：BACKGROUND:Exposure to carbon black nanoparticles (CBNPs), a well-known industrial production, promotes pulmonary toxicity through inflammation and oxidative stress. Recent studies show that some polyphenols exert their antioxidant properties through regulation of protein kinase C-α (PKC-α) and NADPH oxidase (Nox) sign...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-018-2278-6
更新日期：2018-07-09 00:00:00
abstract：BACKGROUND:In our previous studies, it was evident that the dichloromethane-methanol (1:1 v/v) stem barks extract of Polyscias fulva and fractions (ethyl acetate, n-butanol and residue) demonstrated interesting antidermatophytic activities. So, as a continuity of that, this work aimed at identifying active principles w...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-015-0541-7
更新日期：2015-02-15 00:00:00
abstract：BACKGROUND:Phaleria macrocarpa (Scheff.) Boerl (Thymelaceae) originates from Papua Island, Indonesia and grows in tropical areas. The different parts of the fruit of P. macrocarpa were evaluated for antioxidant, anti-inflammatory, and cytotoxic activities. METHODS:Phaleria macrocarpa fruit were divided into pericarp, ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-11-110
更新日期：2011-11-09 00:00:00
abstract：BACKGROUND:Storage lower urinary tract symptoms (LUTS) including overactive bladder (OAB) and urinary incontinence (UI) affect millions of people worldwide, significantly impacting quality of life. Plant based medicines have been documented both empirically and in emerging scientific research to have varying benefits i...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12906-018-2101-4
更新日期：2018-01-31 00:00:00
abstract：BACKGROUND:Complementary and alternative medicine (CAM) is becoming a popular treatment for modulating diverse immune disorders. Phellinus linteus (P. linteus) as one of the CAMs has been used to modulate cancers, inflammation and allergic activities. However, little evidence has been shown about its underlying mechani...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-159
更新日期：2012-09-18 00:00:00
abstract：BACKGROUND:The use of Complementary and Alternative medicine (CAM) has increased over the past years. In Germany, many general practitioners (GPs) use CAM in their daily practice. However, little is known about possible differences of GPs using CAM compared to GPs not using CAM. The aim of the study was to explore diff...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-11-131
更新日期：2011-12-19 00:00:00
abstract：BACKGROUND:Xanthones are a group of oxygen-containing heterocyclic compounds with remarkable pharmacological effects such as anti-cancer, antioxidant, anti-inflammatory, and antimicrobial activities. METHODS:A xanthones extract (81% α-mangostin and 16% γ-mangostin), was prepared by crystallization of a toluene extract...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-104
更新日期：2012-07-20 00:00:00
abstract：BACKGROUND:"Horchata" is an herbal mixture infusion consumed in Southern Ecuador; 66% of its plants are anti-inflammatory medicinal plant, and 51% are analgesics. Anti-inflammatory substances can prevent carcinogenesis mediated by cytotoxic effects and can prevent DNA damage. The aim of this study was to evaluate the c...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-017-2048-x
更新日期：2017-12-19 00:00:00
abstract：BACKGROUND:Flavonoids and other polyphenols play a protective role in liver diseases and possess a high antioxidant capacity. OBJECTIVE:To compare and evaluate the antioxidant and hepatotoprotective activities of 4 deserts plants, Fagonia indica Burm. f., Calotropis procera R.Br., Zygophylum hamiense Schweinf. and Sal...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-015-0919-6
更新日期：2015-11-09 00:00:00
abstract：BACKGROUND:Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring antioxidants of plant origin. The aim of the present study was to evaluate the in vitro antioxidant activities of Spondias pinnata stem bark extract. METHODS:A 70% met...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-8-63
更新日期：2008-12-09 00:00:00
abstract：BACKGROUND:Anoectochilus formosanus has been used as a Chinese folk medicine and is known as the "King of medicine" in Chinese society due to its versatile pharmacological effects such as anti-hypertension, anti-diabetes, anti-heart disease, anti-lung and liver diseases, anti-nephritis and anti-Rheumatoid arthritis. Ki...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-016-1054-8
更新日期：2016-02-25 00:00:00
abstract：BACKGROUND:Labisia pumila, locally known as Kacip Fatimah, is a forest-floor plant that has tremendous potential in the herbal industry. It is one of the five herbal plants identified by the government as one of the national key economic areas to be developed for commercial purposes. There are three varieties of L. pum...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-13-20
更新日期：2013-01-24 00:00:00
abstract：BACKGROUND:The effect and safety of catgut implantation at acupoints o treat allergic rhinitis (ICD-10 code J30.4) remain controversial. Here, we used a sham catgut implantation group to determine whether catgut implantation at acupoints is an effective and safe treatment for allergic rhinitis. METHODS:A randomized do...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12906-016-1400-x
更新日期：2016-11-10 00:00:00
abstract：BACKGROUND:Ginger is a popular spice and food preservative. The rhizomes of the common ginger have been used as traditional medicine to treat various ailments. 6-Shogaol, a pungent compound isolated from the rhizomes of jahe gajah (Zingiber officinale var officinale) has shown numerous pharmacological activities, inclu...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-017-1837-6
更新日期：2017-06-24 00:00:00
abstract：BACKGROUND:Evidence suggests yoga is a safe and effective intervention for the management of physical and psychosocial symptoms associated with musculoskeletal conditions. However, heterogeneity in the components and reporting of clinical yoga trials impedes both the generalization of study results and the replication ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-14-196
更新日期：2014-06-18 00:00:00
abstract：BACKGROUND:Cucumis sativus L. (cucumber), from the family Cucurbitaceae, is a therapeutic plant with various pharmacological benefits, broadly utilized as a part of complementary medicine (e.g., Unani, Ayurveda, Siddha, and Traditional Chinese). In light of past research discoveries, this plant had been chosen to consi...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-018-2176-y
更新日期：2018-04-03 00:00:00
abstract：BACKGROUNDS:Although the physical and mental enhancement effect of essential oils have been proved, the beneficial effect of essential oil in central fatigue remains unclear. In this study, we extracted essential oils from nine aromatic plants to make a compound essential oil, and detected the therapeutic effect of cen...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-018-2375-6
更新日期：2018-11-26 00:00:00
abstract：BACKGROUND:Acupuncture is not considered a conventional therapy for post-stroke sequelae but it might have some additional positive effects on early rehabilitation. We conducted this trial to determine whether acupuncture has additional effects in early comprehensive rehabilitation for acute ischemic stroke and dysfunc...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1186/s12906-016-1193-y
更新日期：2016-07-18 00:00:00
