Abstract：
BACKGROUND:Cyanidin-3-rutinoside (C3R), a naturally occurring anthocyanin, possesses anti-oxidant, anti-hyperglycemic, anti-glycation and cardioprotective properties. However, its mechanisms responsible for anti-hyperlipidemic activity have not been fully identified. The aim of the study was to investigate the lipid-lowering mechanisms of C3R through inhibition of lipid digestion and absorption in vitro. METHODS:The inhibitory activity of C3R against pancreatic lipase and cholesterol esterase was evaluated using enzymatic fluorometric and enzymatic colorimetric assays, respectively. An enzyme kinetic study using Michaelis-Menten and the derived Lineweaver-Burk plot was performed to understand the possible types of inhibition. The formation of cholesterol micelles was determined using the cholesterol assay kit. The bile acid binding was measured using the colorimetric assay. The NBD cholesterol uptake in Caco-2 cells was determined using fluorometric assay. The mRNA expression of cholesterol transporter (Niemann-Pick C1-like 1) was determined by RT-PCR. RESULTS:The results showed that C3R was a mixed-type competitive inhibitor of pancreatic lipase with the IC50 value of 59.4 ± 1.41 μM. Furthermore, C3R (0.125-1 mM) inhibited pancreatic cholesterol esterase about 5-18%. In addition, C3R inhibited the formation of cholesterol micelles and bound to primary and secondary bile acid. In Caco-2 cells, C3R (12.5-100 μM) exhibited a significant reduction in cholesterol uptake in both free cholesterol (17-41%) and mixed micelles (20-30%). Finally, C3R (100 μM) was able to suppress mRNA expression of NPC1L1 in Caco-2 cells after 24 h incubation. CONCLUSIONS:The present findings suggest that C3R acts as a lipid-lowering agent through inhibition of lipid digestion and absorption.
journal_name
BMC Complement Altern Medjournal_title
BMC complementary and alternative medicineauthors
Thilavech T,Adisakwattana Sdoi
10.1186/s12906-019-2664-8subject
Has Abstractpub_date
2019-09-05 00:00:00pages
242issue
1issn
1472-6882pii
10.1186/s12906-019-2664-8journal_volume
19pub_type
杂志文章abstract：BACKGROUND:In traditional Chinese medicine, green tea is considered to have a life-prolonging effect, possibly as a result of its rich content of antioxidant tea polyphenols, and hence has the potential to prevent cancer. This study investigated the role of the major tea secondary plant compound epigallocatechin gallat...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-258
更新日期：2012-12-18 00:00:00
abstract：BACKGROUND:This study was aimed to determination the tumor inhibitory effect and explore the potential mechanisms of Lagopsis supine ethanol extract (Ls) on colorectal cancer. METHODS:The cell growth inhibition experiment of Ls in colorectal cancer cell lines was determined by MTT method in the time course of 24, 48 a...
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journal_title：BMC complementary and alternative medicine
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更新日期：2017-03-29 00:00:00
abstract：BACKGROUND:Recently, considerable attention has been focused on exploring the potential antioxidant properties of plant extracts or isolated products of plant origin. Prunella vulgaris var. lilacina is widely distributed in Korea, Japan, China, and Europe, and it continues to be used to treat inflammation, eye pain, he...
journal_title：BMC complementary and alternative medicine
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doi：10.1186/1472-6882-13-310
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abstract：BACKGROUND:Hepatocellular carcinoma (HCC) is the third leading cause of cancer deaths reported worldwide. The incidence is higher in Asia and Africa, where there is greater endemic prevalence of hepatitis B and C. The devastating outcome of cancer can be minimized only by the use of potent therapeutic agents. Tridham (...
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journal_title：BMC complementary and alternative medicine
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journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,多中心研究,随机对照试验
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journal_title：BMC complementary and alternative medicine
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doi：10.1186/1472-6882-14-244
更新日期：2014-07-16 00:00:00
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journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
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更新日期：2015-09-02 00:00:00
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journal_title：BMC complementary and alternative medicine
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journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2016-07-26 00:00:00
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abstract：BACKGROUNDS:Inflammation is recognized as the key pathological mechanism of type 2 diabetes. The hypoglyceamic effects of berberine (BBR) are related to the inhibition of the inflammatory response, but the mechanism is not completely clear. METHODS:The inflammatory polarization of Raw264.7 cells and primary peritoneal...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
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journal_title：BMC complementary and alternative medicine
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更新日期：2019-04-03 00:00:00
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journal_title：BMC complementary and alternative medicine
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journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1186/1472-6882-8-36
更新日期：2008-07-06 00:00:00
