Abstract：
BACKGROUND:Paclitaxel (taxol) is a potent anticancer drug that is used in the treatment of a wide variety of cancerous. In the present study, we identified a taxol derivative named 7-epi-10-deacetyltaxol (EDT) from the culture of an endophytic fungus Pestalotiopsis microspora isolated from the bark of Taxodium mucronatum. This study was carried out to investigate the effects of fungal EDT on cell proliferation, the induction of apoptosis and the molecular mechanisms of apoptosis in human hepatoma HepG2 cells in vitro. METHODS:The endophytic fungus was identified by traditional and molecular taxonomical characterization and the fungal EDT was purified using column chromatography and confirmed by various spectroscopic and chromatographic comparisons with authentic paclitaxel. We studied the in vitro effects of EDT on HepG2 cells for parameters such as cell cycle distribution, DNA fragmentation, reactive oxygen species (ROS) generation and nuclear morphology. Further, western blot analysis was used to evaluate Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), p38-mitogen activated protein kinase (MAPK) and poly [ADP-ribose] polymerase (PARP) expression. RESULTS:We demonstrate that the fungal EDT exhibited significant in vitro cytotoxicity in HepG2 cells. We investigated cytotoxicity mechanism of EDT in HepG2 cells. The results showed nuclear condensation and DNA fragmentation were observed in cells treated with fungal EDT. Besides, the fungal EDT arrested HepG2 cells at G2/M phase of cell cycle. Furthermore, fungal EDT induced apoptosis in HepG2 cells in a dose-dependent manner associated with ROS generation and increased Bax/Bcl-2 ratio, p38 MAPKs and PARP cleavage. CONCLUSIONS:Our data show that EDT induced apoptotic cell death in HepG2 cells occurs through intrinsic pathway by generation of ROS mediated and activation of MAPK pathway. This is the first report for 7-epi-10-deacetyltaxol (EDT) isolated from a microbial source.
journal_name
BMC Complement Altern Medjournal_title
BMC complementary and alternative medicineauthors
Subban K,Singh S,Subramani R,Johnpaul M,Chelliah Jdoi
10.1186/s12906-017-1993-8subject
Has Abstractpub_date
2017-11-28 00:00:00pages
504issue
1issn
1472-6882pii
10.1186/s12906-017-1993-8journal_volume
17pub_type
杂志文章abstract：BACKGROUND:Antioxidant and anti-inflammatory properties of honey have been largely recognized by various studies. Almost all of the potential benefits are associated with polyphenol content. Honey varieties from the arid region are reported to be rich in polyphenols, but data related to its bioactivity in vitro is grea...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-017-1664-9
更新日期：2017-03-29 00:00:00
abstract：BACKGROUND:Previous experiments have shown that a decoction of Bauhinia forficata leaves reduces the changes in carbohydrate and protein metabolism that occur in rats with streptozotocin-induced diabetes. In the present investigation, the serum activities of enzymes known to be reliable toxicity markers were monitored ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-4-7
更新日期：2004-06-08 00:00:00
abstract：BACKGROUND:Heracleum moellendorffii roots (HM-R) have been long treated for inflammatory diseases such as arthritis, backache and fever. However, an anti-inflammatory effect and the specific mechanism of HM-R were not yet clear. In this study, we for the first time explored the anti-inflammatory of HM-R. METHODS:The c...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-019-2735-x
更新日期：2019-11-12 00:00:00
abstract：BACKGROUND:Rhus verniciflua Stokes (RVS) belongs to the Anacardiaceae family and traditionally used for cancer treatment. RVS and butein, a major compound of RVS, were known to induce apoptosis via AKT inhibition in cancer cells. Thus, in this study, we investigated the effect of RVS and its derivative compounds (fiset...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-016-1103-3
更新日期：2016-04-27 00:00:00
abstract：BACKGROUND:Threatened miscarriage involves vaginal bleeding in a pregnancy that remains viable. This is a common early pregnancy complication with increased risk factors for early pregnancy loss, preterm premature rupture of membranes (PPROM), preterm delivery, low birth weight babies and maternal antepartum haemorrhag...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-20
更新日期：2012-03-22 00:00:00
abstract：BACKGROUND:Dementia is a prevalent neurodegenerative disorder affecting an estimated 24.3 million people across the globe. The burden on those caring for people with dementia is substantial, with widespread implications for the caregiver, the care recipient and the community. Relaxation techniques, such as Transcendent...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12906-015-0666-8
更新日期：2015-05-08 00:00:00
abstract：BACKGROUND:The extracts of the ten selected Sri Lankan medicinal plants have been traditionally used in the treatment of inflammatory mediated diseases. The extracts were investigated for anti-inflammatory and anti-oxidant potential in vitro to identify bio-active extracts for further chemical characterization. METHOD...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-018-2335-1
更新日期：2018-10-03 00:00:00
abstract：BACKGROUND:Anoectochilus formosanus has been used as a Chinese folk medicine and is known as the "King of medicine" in Chinese society due to its versatile pharmacological effects such as anti-hypertension, anti-diabetes, anti-heart disease, anti-lung and liver diseases, anti-nephritis and anti-Rheumatoid arthritis. Ki...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-016-1054-8
更新日期：2016-02-25 00:00:00
abstract：BACKGROUND:This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury. METHODS:VSMCs were treated with different concentrations of GLY, and then analyzed with...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-137
更新日期：2012-08-24 00:00:00
abstract：BACKGROUND:Tianshu capsule (TSC), a formula of traditional Chinese medicine, has been widely used in clinical practice for prophylactic treatment of headaches in China. However, former clinical trials of TSC were small, and lack of a standard set of diagnostic criteria to enroll patients. The study was conducted to re-...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1186/s12906-019-2775-2
更新日期：2019-12-16 00:00:00
abstract：BACKGROUND:Although many physiological functions of Coprinus comatus have been reported, there has been no report on the antinociceptive activity of Coprinus comatus. Therefore, the objective of the present study is to demonstrate the production, isolation, and biological properties of triglycerides (TFC) of the fermen...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-52
更新日期：2012-04-24 00:00:00
abstract：BACKGROUND:Hyaluronidases have been found as the target enzymes in the development of osteoarthritis (OA) disease. While there is still no curative treatment for this disease, recent studies on the treatment of OA were focused on the effectiveness of natural products which are expected to improve the symptoms with mini...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-13-213
更新日期：2013-08-23 00:00:00
abstract：BACKGROUND:To evaluate and compare effects of an integrative treatment (IT), therapeutic acupuncture (TA), and conventional treatment (CT) in alleviating symptoms of anxiety and depression in psychologically distressed primary care patients. METHODS:An open, pragmatic randomized controlled trial comparing the three tr...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,随机对照试验
doi：10.1186/1472-6882-13-308
更新日期：2013-11-07 00:00:00
abstract：BACKGROUND:Although the dopamine precursor L-3, 4-dihydroxyphenylalanine ( l -dopa) remains the gold standard pharmacological therapy for patients with Parkinson's disease (PD), long-term treatment with this drug has been known to result in several adverse effects, including l -dopa-induced dyskinesia (LID). Recently, ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-017-1731-2
更新日期：2017-04-19 00:00:00
abstract：BACKGROUND:Acute postoperative pain remains a major clinical problem that affects patient recovery. Distal acupoint and peri-incisional stimulation are both used for relieving acute postoperative pain in hospital. Our objective was to assess and compare the effects of distal and peri-incisional stimulation on postopera...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,meta分析
doi：10.1186/s12906-019-2583-8
更新日期：2019-07-30 00:00:00
abstract：BACKGROUND:Artemisia scoparia is traditionally used in the local system of medicine in kidney disorders. This study aimed at scrutinizing the nephroprotective prospective of A. scoparia methanol extract against carbon tetrachloride (CCl4) provoked DNA damages and oxidative stress in kidneys of rat. METHODS:Dried aeria...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-016-1137-6
更新日期：2016-05-27 00:00:00
abstract：BACKGROUND:Cedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. The Cedrus deodara total lignans from the pine needles (CTL) were extracted. The aim of the study was to investigate the anticancer potential of the CTL on A549 cell li...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-019-2682-6
更新日期：2019-10-24 00:00:00
abstract：BACKGROUND:Insulin resistance is characterized by deficient responses to insulin in its target tissues. In the present study, we examined the effects of L-Citrulline (L-Cit) on insulin sensitivity and signaling cascades in rat hepatoma H4IIE cells and SHRSP.Z-Leprfa/IzmDmcr rats. METHODS:H4IIE cells were pretreated in...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-015-0706-4
更新日期：2015-06-18 00:00:00
abstract：BACKGROUND:Patients with cancer are vulnerable to depression or other depressive conditions. The aim of this paper was to evaluate the efficacy and safety of Chinese herbal medicine (CHM) for the treatment of depression or depressive symptoms in cancer patients. METHODS:CENTRAL, MEDLINE, EMBASE, PsycINFO, CNKI, VIP, S...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,meta分析
doi：10.1186/s12906-019-2441-8
更新日期：2019-01-23 00:00:00
abstract：BACKGROUND:Uncomplicated chronic rachialgia is a highly prevalent complaint, and one for which therapeutic results are contradictory. The aim of the present study is to evaluate the effectiveness and safety of treatment with auriculopressure, in the primary healthcare sector, carried out by trained healthcare professio...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1186/1472-6882-8-36
更新日期：2008-07-06 00:00:00
abstract：BACKGROUND:Wenshen Jianpi recipe (WSJPR), a blended traditional Chinese medicine, is considered to have the possible beneficial effect on the progression of diabetic nephropathy (DN). This present study was designed to elucidate this protective activity in a rat model with streptozotocin (STZ)-induced DN and to explore...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-019-2598-1
更新日期：2019-07-30 00:00:00
abstract：BACKGROUND:Gentiana floribunda was investigated for the possible hypotensive and vasodilator activities in an attempt to rationalize its traditional use in hypertension. METHODS:The crude extract of Gentiana floribunda (Gf.Cr) was studied in anaesthetized rats and isolated thoracic aorta tissues. RESULTS:Gf.Cr which ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-12-121
更新日期：2012-08-11 00:00:00
abstract：BACKGROUND:Punica granatum (pomegranate), an edible fruit originating in the Middle East, has been used as a traditional medicine for treatment of pain and inflammatory conditions such as peptic ulcer. The numerous risks associated with nonsteroidal anti-inflammatory drugs (NSAIDs) for treatment of pain and inflammatio...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-017-1555-0
更新日期：2017-01-14 00:00:00
abstract：BACKGROUND:Comorbidity of musculoskeletal (MSK) and mental health (MH) problems is common but challenging to treat using conventional approaches. Integration of conventional with complementary approaches (CAM) might help address this challenge. Integration can aim to transform biomedicine into a new health paradigm or ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-018-2349-8
更新日期：2018-10-29 00:00:00
abstract：BACKGROUND:Samsoeum (SSE), a traditional herbal formula, has been widely used to treat cough, fever, congestion, and emesis for centuries. Recent studies have demonstrated that SSE retains potent pharmacological efficiency in anti-allergic and anti-inflammatory reactions. However, the anti-cancer activity of SSE and it...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-13-233
更新日期：2013-09-23 00:00:00
abstract：BACKGROUND:The liver plays an essential role in the body by regulating several important metabolic functions. Liver injury is associated with the distortion of these functions causing many health problems. Pharmaceutical drugs treat liver disorders but cause further damage to it. Hence, herbal drugs are used worldwide ...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/1472-6882-13-160
更新日期：2013-07-05 00:00:00
abstract：BACKGROUND:Our preliminary studies indicated that electroacupuncture (EA) at the ST36 and Ashi acupoints could promote regeneration of the rabbit gastrocnemius (GM) by improving microcirculation perfusion, promoting the recovery of myofiber structures, and inhibiting excessive fibrosis. However, the effects of EA on re...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-015-0601-z
更新日期：2015-03-19 00:00:00
abstract：BACKGROUND:Protein tyrosine phosphatase expressed in insulin-sensitive tissues (such as liver, muscle, and adipose tissue) has a key role in the regulation of insulin signaling and pathway activation, making protein tyrosine phosphatase a promising target for the treatment of type 2 diabetes mellitus and obesity and re...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-016-1296-5
更新日期：2016-08-26 00:00:00
abstract：BACKGROUND:Although a number of studies have been conducted on the prevalence of dietary supplement (DS) use in military personnel, these investigations have not been previously summarized. This article provides a systematic literature review of this topic. METHODS:Literature databases, reference lists, and other sour...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章,meta分析,评审
doi：10.1186/1472-6882-14-143
更新日期：2014-05-02 00:00:00
abstract：BACKGROUND:P-113 (AKRHHGYKRKFH-NH2) is a 12-amino-acid histidine-rich peptide derived from histatin 5 that is highly degradable in high salt concentrations and biological fluids such as serum, plasma and saliva. Nal-P-113, a novel antimicrobial peptide whose histidine residues are replaced by the bulky amino acids β-na...
journal_title：BMC complementary and alternative medicine
pub_type： 杂志文章
doi：10.1186/s12906-017-1931-9
更新日期：2017-08-29 00:00:00
