A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways.

Abstract:

BACKGROUND:This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury. METHODS:VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining. RESULTS:Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis. CONCLUSIONS:These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

authors

Chien YC,Sheu MJ,Wu CH,Lin WH,Chen YY,Cheng PL,Cheng HC

doi

10.1186/1472-6882-12-137

subject

Has Abstract

pub_date

2012-08-24 00:00:00

pages

137

issn

1472-6882

pii

1472-6882-12-137

journal_volume

12

pub_type

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