Abstract:
BACKGROUND:Maintaining the quality of clinical specimens for tuberculosis (TB) testing is a major challenge in many high TB burden-limited resources countries. Sample referral systems in low and middle income countries are often weak and the maintenance of the cold-chain challenging and very costly for TB programs. The development of transport media allowing the preservation of samples without refrigeration is critical for increasing access to TB diagnostic services and for reducing the costs related to testing. METHODS:We evaluated the performance of OMNIgene-SPUTUM (OM-S) reagent for the maintenance of Mycobacterium tuberculosis (MTB) viability in sputum samples in the absence of refrigeration and its capacity to stabilize nucleic acid for molecular testing. A total of 329 sputum specimens from presumptive TB cases collected at the National Reference Laboratory in Tirana, Albania, were either decontaminated by a conventional method or processed with OM-S reagent and stored at room temperature. Samples in OM-S were shipped to the Supranational Reference Laboratory in Milan, Italy, at various times and processed for liquid culture. RESULTS:Our data show that OM-S maintains MTB viability for at least three weeks in the absence of refrigeration and improves the quality of culture resulting in a contamination rate lower than 0.5%. However, a significant delay in the time to culture positivity was observed for samples stored for more than two weeks in OM-S. CONCLUSIONS:Overall, OM-S offers multiple benefits both at laboratory and TB national program level by increasing the availability to quality diagnostics, promoting access to health care services and strengthening TB patient care especially in hard to reach populations.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Tagliani E,Alagna R,Tafaj S,Hafizi H,Cirillo DMdoi
10.1186/s12879-017-2756-3subject
Has Abstractpub_date
2017-10-02 00:00:00pages
663issue
1issn
1471-2334pii
10.1186/s12879-017-2756-3journal_volume
17pub_type
杂志文章abstract:BACKGROUND:Although breast-feeding accounts for 15-20% of mother-to-child transmission (MTCT) of HIV, it is not prohibited in some developing countries because of the higher mortality associated with not breast-feeding. We assessed the potential impact, on HIV infection and infant mortality, of a recommendation for sho...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-8-66
更新日期:2008-05-16 00:00:00
abstract:BACKGROUND:The Australian state of Victoria, with 5.2 million residents, enforced home quarantine during a H1N1 pandemic in 2009. The strategy was targeted at school children. The objective of this study was to investigate the extent to which parents' access to paid sick leave or paid carer's leave was associated with ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-311
更新日期:2012-11-20 00:00:00
abstract:BACKGROUND:There is conclusive evidence that human papillomavirus (HPV) infections of the cervix are a necessary cause of cervical cancer. In Italy there are consistent data of HPV prevalence in women aged 25 - 64 years, but there is limited data for younger women. The objective of this on-going 3-year prospective coho...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-10-157
更新日期:2010-06-07 00:00:00
abstract::Detection of the Mycobacterium tuberculosis cell wall antigen lipoarabinomannan (LAM) in urine permits diagnoses of tuberculosis (TB) to be made in HIV-infected patients with advanced immunodeficiency. This can be achieved at the point-of-care within just 30 minutes using the Determine TB-LAM, which is a commercially ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-407
更新日期:2013-09-03 00:00:00
abstract:BACKGROUND:To explore the clinical features and CT findings of clinically cured coronavirus disease 2019 (COVID-19) patients with viral RNA positive anal swab results after discharge. METHODS:Forty-two patients with COVID-19 who were admitted to Yongzhou Central Hospital, Hunan, China, between January 20, 2020, and Ma...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05363-2
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:The major infectious diseases of hepatitis B has constituted an acute public health challenge in China. An effective and affordable HBV control model is urgently needed. A national project of Community-based Collaborative Innovation HBV (CCI-HBV) demonstration areas has optimized the existing community healt...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4150-9
更新日期:2019-07-12 00:00:00
abstract:BACKGROUND:To provide a clear picture of the current hepatitis B situation, the authors performed a systematic review to estimate the age- and region-specific prevalence of chronic hepatitis B (CHB) in Turkey. METHODS:A total of 339 studies with original data on the prevalence of hepatitis B surface antigen (HBsAg) in...
journal_title:BMC infectious diseases
pub_type: 杂志文章,meta分析,评审
doi:10.1186/1471-2334-11-337
更新日期:2011-12-12 00:00:00
abstract:BACKGROUND:In 2007, Park et al. identified a novel serotype among Streptococcus pneumoniae serogroup 6 which they named serotype 6C. The aim of this study was to evaluate with the Neufeld test a novel S. pneumoniae serotype 6C type specific polyclonal antiserum. In addition, serotype 6C isolates found in Denmark in 200...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-10-282
更新日期:2010-09-24 00:00:00
abstract:BACKGROUND:Lyme disease, a bacterial infection with the tick-borne spirochete Borrelia burgdorferi, can cause early and late manifestations. The category of probable Lyme disease was recently added to the CDC surveillance case definition to describe patients with serologic evidence of exposure and physician-diagnosed d...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-173
更新日期:2012-08-01 00:00:00
abstract:BACKGROUND:The C - reactive protein (CRP) response is often measured in patients with active tuberculosis (TB) yet little is known about its relationship to clinical features in TB, or whether responses differ between ethnic groups or with different Mycobacterium tuberculosis (M.tb) strain types. We report the relation...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1612-1
更新日期:2016-06-10 00:00:00
abstract:BACKGROUND:Raw seafood consumption was identified as the major risk factor for hepatitis A during the large epidemic of 1996 and 1997 in Puglia (South Italy). In Puglia, vaccination for toddlers and preadolescents has been recommended since 1998.The aim of the study was to evaluate the incidence, seroprevalence, molecu...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-271
更新日期:2012-10-25 00:00:00
abstract:BACKGROUND:Pneumocystis pneumonia (PCP) may develop as a clinical manifestation of nosocomial pneumonia by means of either reactivation of resident P. jirovecii or de novo infection. However, there have been no studies describing the clinical characteristics of hospital-onset PCP. METHODS:A retrospective review of med...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0847-6
更新日期:2015-02-26 00:00:00
abstract:BACKGROUND:The urine lipoarabinomannan (LAM) strip test has been suggested as a new point-of-care test for active tuberculosis (TB) among human immunodeficiency virus (HIV) infected individuals. It has been questioned if infections with nontuberculous mycobacteria (NTM) affect assay specificity. We set forth to investi...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章
doi:10.1186/s12879-014-0655-4
更新日期:2014-12-04 00:00:00
abstract:BACKGROUND:HIV affects the central nervous system resulting in HIV associated neurocognitive impairment (NCI) in approximately 50% of people living with HIV. It typically affects memory, learning, working memory, fine motor skills, speed of information processing, verbal fluency and executive functioning cognitive doma...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05090-8
更新日期:2020-05-29 00:00:00
abstract:BACKGROUND:Extended use of oseltamivir in an immunocompromised host could reportedly induce neuraminidase gene mutation possibly leading to oseltamivir-resistant influenza A/H3N2 virus. To our knowledge, no report is available on the clinical course of a severely immunocompromised patient with a dual E119D/R292K neuram...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05205-1
更新日期:2020-07-06 00:00:00
abstract:BACKGROUND:Substantial numbers of patients are now receiving either immunosuppressive therapies or chemotherapy. There are significant risks in such patients of developing opportunistic infections or re-activation of latent infections, with higher associated morbidity and mortality. The aim of this quality improvement ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05082-8
更新日期:2020-05-20 00:00:00
abstract:BACKGROUND:Community-acquired pneumonia (CAP) remains a major cause of morbidity and mortality throughout the world. Telithromycin (a new ketolide) has shown good in vitro activity against the key causative pathogens of CAP, including S pneumoniae resistant to penicillin and/or macrolides. METHODS:The efficacy and saf...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1186/1471-2334-5-43
更新日期:2005-05-31 00:00:00
abstract:BACKGROUND:Qinghai-Tibetan Plateau of China is known to be the plague endemic region where marmot (Marmota himalayana) is the primary host. Human plague cases are relatively low incidence but high mortality, which presents unique surveillance and public health challenges, because early detection through surveillance ma...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-382
更新日期:2014-07-10 00:00:00
abstract:BACKGROUND:Cellulose acetate phthalate (CAP) has been used for several decades in the pharmaceutical industry for enteric film coating of oral tablets and capsules. Micronized CAP, available commercially as "Aquateric" and containing additional ingredients required for micronization, used for tablet coating from water ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-3-27
更新日期:2003-11-14 00:00:00
abstract:BACKGROUND:Chronic hepatitis C virus (HCV) infection can progress to cirrhosis and end-stage liver disease in a substantial proportion of patients. The infection is frequently asymptomatic, leaving many infected individuals unaware of the diagnosis until complications occur. This advocates the screening of healthy indi...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3832-7
更新日期:2019-02-26 00:00:00
abstract:BACKGROUND:High blood levels of soluble urokinase Plasminogen Activator Receptor (suPAR) are associated with poor outcomes in human immunodeficiency-1 (HIV-1) infected individuals. Research on the clinical value of suPAR in HIV-1 infection led to the development of the suPARnostic(R) assay for commercial use in 2006. T...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-7-134
更新日期:2007-11-16 00:00:00
abstract:BACKGROUND:NOD2, an intracellular pathogen recognition sensor, modulates innate defences to muropeptides derived from various bacterial species, including Mycobacterium tuberculosis (MTB). Experimentally, NOD2 attenuates two key putative mycobactericidal mechanisms. TNF-alpha synthesis is markedly reduced in MTB-antige...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-7-96
更新日期:2007-08-16 00:00:00
abstract:BACKGROUND:Bacterial resistance to first line antibiotics used to treat community-onset urinary tract infections (UTIs) continues to emerge. We sought to determine the association between extended-spectrum cephalosporin resistance (ESC-R) and recurrence among Enterobacteriaceae (EB) UTIs. METHODS:A retrospective cohor...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3804-y
更新日期:2019-02-14 00:00:00
abstract:BACKGROUND:Regardless of chronic treatment with antiretroviral therapy (ART), the switching rate for ART regarding anchor drugs has not been articulated in real-world clinical-settings in Japan. We assessed switch rates and time-to-switch of ART regimens according to anchor drug classes (integrase strand transfer inhib...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4129-6
更新日期:2019-06-10 00:00:00
abstract:BACKGROUND:Guidelines from the Infectious Diseases Society of America/The American Thoracic Society (IDSA/ATS) provide recommendations for diagnosis and treatment of ventilator-associated pneumonia (VAP). However, the mere presence of guidelines is rarely sufficient to promote widespread adoption and uptake. Using the ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1665-1
更新日期:2016-07-22 00:00:00
abstract:BACKGROUND:Intracranial infection after puncture of cerebral hematoma in patients with intracerebral hemorrhage is very common in the department of neurosurgery, yet the relevant risks remain unknown. We attempted to analyze the risk factors of intracranial infection after puncture of cerebral hematoma, to provide insi...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05630-2
更新日期:2020-12-11 00:00:00
abstract:BACKGROUND:This epidemiological study was carried out in Sfax (south of Tunisia) and focused on genital Chlamydia trachomatis (C. trachomatis) genovar distribution. METHODS:One hundred and thirty seven genital samples from 4067 patients (4.2%) attending the Habib Bourguiba University hospital of Sfax over 12 years (fr...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-333
更新日期:2012-11-30 00:00:00
abstract:BACKGROUND:Malnutrition and malaria frequently coexist in sub-Saharan African countries. Studies on efficacy of antimalarial treatments usually follow the WHO standardized protocol in which severely malnourished children are systematically excluded. Few studies have assessed the efficacy of chloroquine, sulfadoxine-pyr...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1186/s12879-015-0963-3
更新日期:2015-06-12 00:00:00
abstract:BACKGROUND:For approximately 24 years the AIDS pandemic has claimed approximately 30 million lives, causing approximately 14,000 new HIV-1 infections daily worldwide in 2003. About 80% of infections occur by heterosexual transmission. In the absence of vaccines, topical microbicides, expected to block virus transmissio...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-4-41
更新日期:2004-10-14 00:00:00
abstract:BACKGROUND:The major factors contributing for nerve damage and permanent disabilities in leprosy are type 1 or reversal reactions (RR) and type 2 or erythema nodosum leprosum (ENL). Gene profiling of leprosy reactions have shown that different pathways are activated during the course of reactions, which is consistent w...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3348-6
更新日期:2018-08-24 00:00:00