Nucleolar and spindle associated protein 1 promotes the aggressiveness of astrocytoma by activating the Hedgehog signaling pathway.

Abstract:

BACKGROUND:The prognosis of human astrocytoma is poor, and the molecular alterations underlying its pathogenesis still needed to be elucidated. Nucleolar and spindle associated protein 1 (NUSAP1) was observed in several types of cancers, but its role in astrocytoma remained unknown. METHODS:The expression of NUSAP1 in astrocytoma cell lines and tissues were measured with western blotting and Real-Time PCR. Two hundred and twenty-one astrocytoma tissue samples were analyzed by immunochemistry to demonstrate the correlation between the NUSAP1 expression and clinicopathological characteristics. 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay, colony formation, transwell matrix penetration assay, wound healing assay and anchorage-independent growth assay were used to investigate the biological effect of NUSAP1 in astrocytoma. An intracranial brain xenograft tumor model was used to confirm the oncogenic role of NUSAP1 in human astrocytoma. Luciferase reporter assay was used to investigate the effect of NUSAP1 on Hedgehog signaling pathway. RESULTS:NUSAP1 was markedly overexpressed in astrocytoma cell lines and tissues compared with normal astrocytes and brain tissues. NUSAP1 was found to be overexpressed in 152 of 221 (68.78%) astrocytoma tissues, and was significantly correlated to poor survival. Further, ectopic expression or knockdown of NUSAP1 significantly promoted or inhibited, respectively, the invasive ability of astrocytoma cells. Moreover, intracranial xenografts of astrocytoma cells engineered to express NUSAP1 were highly invasive compared with the parental cells. With regard to its molecular mechanism, upregulation of NUSAP1 in astrocytoma cells promoted the nuclear translocation of GLI family zinc finger 1 (GLI1) and upregulated the downstream genes of the Hedgehog pathway. CONCLUSION:These findings indicate that NUSAP1 contributes to the progression of astrocytoma by enhancing tumor cell invasiveness via activation of the Hedgehog signaling pathway, and that NUSAP1 might be a potential prognostic biomarker as well as a target in astrocytoma.

journal_name

J Exp Clin Cancer Res

authors

Wu X,Xu B,Yang C,Wang W,Zhong D,Zhao Z,He L,Hu Y,Jiang L,Li J,Song L,Zhang W

doi

10.1186/s13046-017-0597-y

subject

Has Abstract

pub_date

2017-09-12 00:00:00

pages

127

issue

1

eissn

0392-9078

issn

1756-9966

pii

10.1186/s13046-017-0597-y

journal_volume

36

pub_type

杂志文章
  • ERRγ target genes are poor prognostic factors in Tamoxifen-treated breast cancer.

    abstract:BACKGROUND:One-third of estrogen (ER+) and/or progesterone receptor-positive (PGR+) breast tumors treated with Tamoxifen (TAM) do not respond to initial treatment, and the remaining 70% are at risk to relapse in the future. Estrogen-related receptor gamma (ESRRG, ERRγ) is an orphan nuclear receptor with broad, structur...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-015-0150-9

    authors: Madhavan S,Gusev Y,Singh S,Riggins RB

    更新日期:2015-05-15 00:00:00

  • CCDC88A, a prognostic factor for human pancreatic cancers, promotes the motility and invasiveness of pancreatic cancer cells.

    abstract:BACKGROUND:Coiled-Coil Domain Containing 88A (CCDC88A) was identified as a substrate of the serine/threonine kinase Akt that is capable of binding to the actin cytoskeleton. The aim of this study was to investigate the potential role of CCDC88A in the migration and invasiveness of pancreatic ductal adenocarcinoma (PDAC...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-016-0466-0

    authors: Tanouchi A,Taniuchi K,Furihata M,Naganuma S,Dabanaka K,Kimura M,Watanabe R,Kohsaki T,Shimizu T,Saito M,Hanazaki K,Saibara T

    更新日期:2016-12-05 00:00:00

  • Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer patients.

    abstract:BACKGROUND:To determine the expression of bone morphogenetic protein-2 (BMP-2) and its receptors BMPRIA, BMPRIB, and BMPRII in epithelial ovarian cancer (EOC) and to analyze their influence on the prognosis of ovarian cancer patients. METHODS:Semi-quantitative RT-PCR and western blot were applied to detect the express...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-29-85

    authors: Ma Y,Ma L,Guo Q,Zhang S

    更新日期:2010-06-30 00:00:00

  • Efficient inhibition of murine breast cancer growth and metastasis by gene transferred mouse survivin Thr34-->Ala mutant.

    abstract:BACKGROUND:Metastasis in breast cancer is a vital concern in treatment because most women with primary breast cancer have micrometastases to distant sites at diagnosis. As a member of the inhibitor of apoptosis protein (IAP) family, survivin has been proposed as an attractive target for new anticancer interventions. In...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-27-46

    authors: Peng XC,Yang L,Yang LP,Mao YQ,Yang HS,Liu JY,Zhang DM,Chen LJ,Wei YQ

    更新日期:2008-09-25 00:00:00

  • A synthetic cell-penetrating peptide derived from nuclear localization signal of EPS8 exerts anticancer activity against acute myeloid leukemia.

    abstract:BACKGROUND:Oncogenic roles of epidermal growth factor receptor pathway substrate no.8 (EPS8) have been widely reported in various tumors, making targeting of EPS8 an appealing prospect. Here, we describe the role of EPS8 in acute myeloid leukemia (AML) and consider the potential of EPS8 as an anti-AML target. Nuclear l...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-018-0682-x

    authors: Chen Y,Xie X,Wu A,Wang L,Hu Y,Zhang H,Li Y

    更新日期:2018-01-22 00:00:00

  • Cdk1/P34Cdc2 and P21waf expression in colorectal adenomas and carcinomas.

    abstract::p21WAF1,CIP1,SDI 1 is a specific inhibitor of cyclin-dependent kinase and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Cdk1/p34cdc2 plays a crucial role during mitosis. The immunoreactivity of p21 WAF1,CIP1,SDI 1 and cdk1/p34cdc2 was evaluated in adenomas and adenocarcinomas of the hum...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Egilmez R,Elagöz S,Kanik EA

    更新日期:2001-12-01 00:00:00

  • Over-expression of long noncoding RNA BANCR inhibits malignant phenotypes of human bladder cancer.

    abstract:BACKGROUND:Accumulating evidences indicated that lncRNAs play crucial regulatory roles in oncogenesis and progression of cancers. BRAF activated non-coding RNA (BANCR) has been identified to contribute to the progression of some human cancers. However, the relationship between BANCR and bladder cancer (BC) is largely u...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-016-0397-9

    authors: He A,Liu Y,Chen Z,Li J,Chen M,Liu L,Liao X,Lv Z,Zhan Y,Zhuang C,Lin J,Huang W,Mei H

    更新日期:2016-08-11 00:00:00

  • CMTM4 is frequently downregulated and functions as a tumour suppressor in clear cell renal cell carcinoma.

    abstract:BACKGROUND:Chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (CMTM) is a gene family involved in multiple malignancies. CMTM4 is a member of this family and is located at chromosome 16q22.1, a locus that harbours a number of tumour suppressor genes. It has been defined as a regulator of ce...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-015-0236-4

    authors: Li T,Cheng Y,Wang P,Wang W,Hu F,Mo X,Lv H,Xu T,Han W

    更新日期:2015-10-16 00:00:00

  • Primary scrotal malignant melanoma. Report of 2 cases and review of the literature.

    abstract::Tumours involving the scrotum are rare and primary malignant melanoma is among the rarest of these lesions. Between 1949 and 1996 only 7 cases have been reported. We present two cases of scrotal melanoma. The current recommendations and management strategies are discussed on the basis of a review of the literature. ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:

    authors: Berkmen F,Tandoğdu R,Ardjçoğlu A

    更新日期:1998-03-01 00:00:00

  • LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett's esophagus?

    abstract:BACKGROUND:Investigation of the expression of an intestinal stem cell marker in esophageal adenocarcinomas (EAC) with and without Barrett's Esophagus (BE), with respect to a cancer stem cell (CSC) hypothesis. MATERIALS AND METHODS:Expression of a putative intestinal stem cell marker LgR5 was analyzed in esophageal can...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-30-23

    authors: von Rahden BH,Kircher S,Lazariotou M,Reiber C,Stuermer L,Otto C,Germer CT,Grimm M

    更新日期:2011-02-23 00:00:00

  • Mucin 17 inhibits the progression of human gastric cancer by limiting inflammatory responses through a MYH9-p53-RhoA regulatory feedback loop.

    abstract:BACKGROUND:Mucins are key components of the mucosal barrier in the stomach that protects epithelia from carcinogenic effects of chronic inflammation. Analysis of The Cancer Genome Atlas database indicated that mucin-17 (MUC17) was more highly expressed in gastric cancer (GC) specimens, with favourable prognosis for pat...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1279-8

    authors: Yang B,Wu A,Hu Y,Tao C,Wang JM,Lu Y,Xing R

    更新日期:2019-07-01 00:00:00

  • Clinically meaningful response to the EGFR tyrosine kinase inhibitor gefitinib ('Iressa', ZD1839) in non small cell lung cancer.

    abstract::Preclinical research and ongoing clinical trials are validating epidermal growth factor receptor (EGFR) as a suitable molecular target in cancer therapy. Here we report the case of a 39-year-old non-smoking man with heavily pretreated stage IV bronchioloalveolar NSCLC who was treated with gefitinib ('Iressa', ZD1839) ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Gelibter A,Ceribelli A,Milella M,Mottolese M,Vocaturo A,Cognetti F

    更新日期:2003-09-01 00:00:00

  • Breast carcinomas with low amplified/equivocal HER2 by Ish: potential supporting role of multiplex ligation-dependent probe amplification.

    abstract:BACKGROUND:This is a retrospective cross sectional study aimed to verify whether Multiplex Ligation-dependent Probe Amplification (MLPA), a quantitative molecular assay, may represent a valuable reflex test in breast cancer with equivocal HER2 expression by immunohistochemistry and HER2 gene signals/nucleus (s/n) rangi...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-017-0613-2

    authors: Ercolani C,Marchiò C,Di Benedetto A,Fabi A,Perracchio L,Vici P,Sperati F,Buglioni S,Arena V,Pescarmona E,Sapino A,Terrenato I,Mottolese M

    更新日期:2017-10-13 00:00:00

  • Human mammaglobin mRNA is a reliable molecular marker for detecting occult breast cancer cells in peripheral blood.

    abstract::Occult carcinoma cells in peripheral blood of breast cancer (BC) patients is generally associated with poor disease prognosis. Reverse transcriptase-polymerase chain reaction (RT-PCR) is a sensitive method for revealing rare circulating cancer cells. The target mRNA must be carefully chosen, as it must be expressed on...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Roncella S,Ferro P,Bacigalupo B,Pronzato P,Tognoni A,Falco E,Gianquinto D,Ansaldo V,Dessanti P,Fais F,Rosai J,Fedeli F

    更新日期:2005-06-01 00:00:00

  • TRPM7 promotes the epithelial-mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling.

    abstract:BACKGROUND:The epithelial-mesenchymal transition (EMT) is crucial for metastasis and positively regulated by calcium-related signaling. The melastatin-related transient receptor potential 7 (TRPM7) regulates a non-selective cation channel and promotes cancer metastasis. However, the mechanisms underlying the action of ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1061-y

    authors: Liu L,Wu N,Wang Y,Zhang X,Xia B,Tang J,Cai J,Zhao Z,Liao Q,Wang J

    更新日期:2019-02-28 00:00:00

  • The effects and the mechanisms of autophagy on the cancer-associated fibroblasts in cancer.

    abstract::Cancer-associated fibroblasts (CAFs) plays an essential role in cancer cell growth, metabolism and immunoreaction. Autophagy is an intracellular self-degradative process that balances cell energy source and regulates tissue homeostasis. Targeting autophagy has gained interest with multiple preclinical and clinical tri...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-019-1172-5

    authors: Yan Y,Chen X,Wang X,Zhao Z,Hu W,Zeng S,Wei J,Yang X,Qian L,Zhou S,Sun L,Gong Z,Xu Z

    更新日期:2019-04-23 00:00:00

  • m6A modification in RNA: biogenesis, functions and roles in gliomas.

    abstract::The chemical modification of RNA is a newly discovered epigenetic regulation mechanism in cells and plays a crucial role in a variety of biological processes. N6-methyladenine (m6A) mRNA modification is the most abundant form of posttranscriptional RNA modification in eukaryotes. Through the development of m6A RNA seq...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-020-01706-8

    authors: Zhang Y,Geng X,Li Q,Xu J,Tan Y,Xiao M,Song J,Liu F,Fang C,Wang H

    更新日期:2020-09-17 00:00:00

  • FGFs/FGFRs-dependent signalling in regulation of steroid hormone receptors - implications for therapy of luminal breast cancer.

    abstract::Stromal stimuli mediated by growth factor receptors, leading to ligand-independent activation of steroid hormone receptors, have long been implicated in development of breast cancer resistance to endocrine therapy. Mutations in fibroblast growth factor receptor (FGFR) genes have been associated with a higher incidence...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-019-1236-6

    authors: Piasecka D,Braun M,Kitowska K,Mieczkowski K,Kordek R,Sadej R,Romanska H

    更新日期:2019-05-29 00:00:00

  • Viruses in human oral cancers.

    abstract::The role of viruses in the etiology of human oral cancer is critically reviewed. Available evidences show a positive correlation for human oral cancer with human papilloma virus (HPV), herpes simplex virus (HSV) and human herpes virus type-6 (HHV-6), having strongest association with HPV. These viruses may act alone o...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:

    authors: Vasudevan DM,Vijayakumar T

    更新日期:1998-03-01 00:00:00

  • Transfection of mammalian cells by the methods of receptor mediated gene transfer and particle bombardment.

    abstract::The efficacy of currently developed methods for gene transfer into mammalian cells depends primarily on the transfection technique, and also on the type of targeted cells. Considering the importance of gene transfer in the creation of gene therapies, our study was aimed at the assessment of transfection capacity of re...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Novaković S,Knezević M,Golouh R,Jezersek B

    更新日期:1999-12-01 00:00:00

  • Dynamic progression of an intraperitoneal xenograft model of human ovarian cancer and its potential for preclinical trials.

    abstract::This study was conducted to establish a feasible intraperitoneal xenograft model in nude mice which mimicked the dynamic progression of human ovarian cancer and to explore its potential for preclinical trials. A human ovarian tumor line SKOV3 was originally injected s.c. to develop tumors; then the tumors were harvest...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Lin XJ,Chen XC,Wang L,Wei YQ,Kan B,Wen YJ,He X,Zhao X

    更新日期:2007-12-01 00:00:00

  • An embryo-specific expressing TGF-β family protein, growth-differentiation factor 3 (GDF3), augments progression of B16 melanoma.

    abstract::Malignant tumor cells often express embryonic antigens which share the expression with embryonic stem (ES) cells. The embryonic antigens are usually encoded by ES cell-specific genes, a number of which are associated with tumorigenesis and/or tumor progression. We examined the expression of ES cell-specific genes in t...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-29-135

    authors: Ehira N,Oshiumi H,Matsumoto M,Kondo T,Asaka M,Seya T

    更新日期:2010-10-15 00:00:00

  • Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression.

    abstract:BACKGROUND:Localized C3 deposition is a well-known factor of inflammation. However, its role in oncoprogression of gastric cancer (GC) remains obscured. This study aims to explore the prognostic value of C3 deposition and to elucidate the mechanism of C3-related oncoprogression for GC. METHODS:From August to December ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 临床试验,杂志文章

    doi:10.1186/s13046-019-1514-3

    authors: Yuan K,Ye J,Liu Z,Ren Y,He W,Xu J,He Y,Yuan Y

    更新日期:2020-01-13 00:00:00

  • Molecular genetics of prostate cancer: clinical translational opportunities.

    abstract::Prostate cancer (PC) development reflects a complex sequence of biologic and molecular events. Several inheritable and somatic genetic changes have been identified. The knowledge of the molecular basis of PC can improve our understanding of the causes of this common cancer and provide information on prognosis and trea...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:

    authors: Vecchione A,Gottardo F,Gomella LG,Wildemore B,Fassan Ms,Bragantini E,Pagano F,Baffa R

    更新日期:2007-03-01 00:00:00

  • Inhibition of ATRA-induced myeloid differentiation in acute promyelocytic leukemia by a new protein tyrosine phosphatase inhibitor, 3,4-dephostatin.

    abstract::We previously reported that the expression and the activity of SHP-1, a non-transmembrane protein tyrosine phosphatase (PTPase) increased during myeloid differentiation of an acute promyelocytic leukemia cell line (HT93) induced by all-trans retinoic acid (ATRA). To examine whether inhibition of SHP-1 activity attenua...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Uesugi Y,Fuse I,Toba K,Kishi K,Hashimoto S,Furukawa T,Narita M,Takahashi M,Aizawa Y

    更新日期:2000-09-01 00:00:00

  • Tumor-induced lymphangiogenesis in cervical lymph nodes in oral melanoma-bearing mice.

    abstract:BACKGROUND:Metastasis via the lymphatic system is promoted by lymphangiogenesis. Alterations of the lymphatic channels during the progression of metastasis to regional lymph nodes (LNs) remain unexplored. To examine whether tumor-induced LN lymphangiogenesis controls metastasis to regional LNs, we investigated cervical...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-31-83

    authors: Ozasa R,Ohno J,Iwahashi T,Taniguchi K

    更新日期:2012-10-02 00:00:00

  • The Chk1 inhibitor SAR-020106 sensitizes human glioblastoma cells to irradiation, to temozolomide, and to decitabine treatment.

    abstract:BACKGROUND:Glioblastoma is the most common and aggressive brain tumour in adults with a median overall survival of only 14 months after standard therapy with radiation therapy (IR) and temozolomide (TMZ). In a novel multimodal treatment approach we combined the checkpoint kinase 1 (Chk1) inhibitor SAR-020106 (SAR), dis...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1434-2

    authors: Patties I,Kallendrusch S,Böhme L,Kendzia E,Oppermann H,Gaunitz F,Kortmann RD,Glasow A

    更新日期:2019-10-21 00:00:00

  • Glucose-6-phosphate dehydrogenase blockade potentiates tyrosine kinase inhibitor effect on breast cancer cells through autophagy perturbation.

    abstract:BACKGROUND:Glucose-6-phospate dehydrogenase (G6PD) is the limiting enzyme of the pentose phosphate pathway (PPP) correlated to cancer progression and drug resistance. We previously showed that G6PD inhibition leads to Endoplasmic Reticulum (ER) stress often associated to autophagy deregulation. The latter can be induce...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1164-5

    authors: Mele L,la Noce M,Paino F,Regad T,Wagner S,Liccardo D,Papaccio G,Lombardi A,Caraglia M,Tirino V,Desiderio V,Papaccio F

    更新日期:2019-04-12 00:00:00

  • Primary hepatic endocrinomas (carcinoids and variant neoplasms). A statistical evaluation of 126 reported cases.

    abstract::This study was undertaken to evaluate the statistical results of a large collected series of primary hepatic endocrinomas including carcinoids from extensive clinicopathologic aspect, and to provide the information to investigators engaged in this particular field of research. Primary hepatic endocrinomas variously te...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Soga J

    更新日期:2002-12-01 00:00:00

  • Long noncoding RNA MAPKAPK5-AS1 promotes colorectal cancer progression by cis-regulating the nearby gene MK5 and acting as a let-7f-1-3p sponge.

    abstract:BACKGROUND:Long noncoding RNAs (lncRNAs) are considered critical regulators in cancers; however, the clinical significance and mechanisms of MAPKAPK5-AS1 (hereinafter referred to as MK5-AS1) in colorectal cancer (CRC) remain mostly unknown. METHODS:In this study, quantitative real-time PCR (qPCR) and western blotting ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01633-8

    authors: Yang T,Chen WC,Shi PC,Liu MR,Jiang T,Song H,Wang JQ,Fan RZ,Pei DS,Song J

    更新日期:2020-07-20 00:00:00