Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression.

Abstract:

BACKGROUND:Localized C3 deposition is a well-known factor of inflammation. However, its role in oncoprogression of gastric cancer (GC) remains obscured. This study aims to explore the prognostic value of C3 deposition and to elucidate the mechanism of C3-related oncoprogression for GC. METHODS:From August to December 2013, 106 GC patients were prospectively included. The regional expression of C3 and other effectors in gastric tissues were detected by WB, IHC, qRT-PCR and other tests. The correlation of localized C3 deposition and oncologic outcomes was determined by 5-year survival significance. Human GC and normal epithelial cell lines were employed to detect a relationship between C3 and STAT3 signaling pathway in vitro experiments. RESULTS:C3 and C3a expression were markedly enhanced in GC tissues at both mRNA and protein levels compared with those in paired nontumorous tissues. According to IHC C3 score, 65 (61.3%) and 41 (38.7%) patients had high and low C3 deposition, respectively. C3 deposition was negatively correlated with plasma levels of C3 and C3a (both P < 0.001) and positively correlated with pathological T and TNM stages (both P < 0.001). High C3 deposition was identified as an independent prognostic factor of poor 5-year overall survival (P = 0.045). In vitro C3 administration remarkably enhanced p-JAK2/p-STAT3 expression in GC cell lines but caused a reduction of such activation when pre-incubated with a C3 blocker. Importantly, C3 failed to activate such signaling in cells pre-treated with a JAK2 inhibitor. CONCLUSIONS:Localized C3 deposition in the tumor microenvironment is a relevant immune signature for predicting prognosis of GC. It may aberrantly activate JAK2/STAT3 pathway allowing oncoprogression. TRIAL REGISTRATION:ClinicalTrials.gov, NCT02425930, Registered 1st August 2013.

journal_name

J Exp Clin Cancer Res

authors

Yuan K,Ye J,Liu Z,Ren Y,He W,Xu J,He Y,Yuan Y

doi

10.1186/s13046-019-1514-3

subject

Has Abstract

pub_date

2020-01-13 00:00:00

pages

9

issue

1

eissn

0392-9078

issn

1756-9966

pii

10.1186/s13046-019-1514-3

journal_volume

39

pub_type

临床试验,杂志文章
  • Relationship between human mammaglobin mRNA expression in breast cancer tissue and clinico-pathologic features of the tumors.

    abstract::Human mammaglobin (hMAM) has recently been recognized as a breast associated glycoprotein. Although the biological role of hMAM is unknown, it has been previously reported that hMAM gene expression is a marker of low biological and clinical aggressiveness of breast cancer (BC). In this study, 148 cases of BC tissues w...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Roncella S,Ferro P,Bacigalupo B,Dessanti P,Giannico A,Gorji N,Moroni M,Tozzini S,Pensa F,Gianquinto D,Fais F,Pronzato P,Fedeli F

    更新日期:2006-03-01 00:00:00

  • Patient-derived xenografts: a relevant preclinical model for drug development.

    abstract::Identifying appropriate preclinical cancer models remains a major challenge in increasing the efficiency of drug development. A potential strategy to improve patient outcomes could be selecting the 'right' treatment in preclinical studies performed in patient-derived xenografts (PDXs) obtained by direct implants of su...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-016-0462-4

    authors: Pompili L,Porru M,Caruso C,Biroccio A,Leonetti C

    更新日期:2016-12-05 00:00:00

  • sE-cadherin serves as a diagnostic and predictive parameter in prostate cancer patients.

    abstract:BACKGROUND:Measurement of prostate-specific antigen (PSA) advanced the diagnostic and prognostic potential for prostate cancer (PCa). However, due to PSA's lack of specificity, novel biomarkers are needed to improve risk assessment and ensure optimal personalized therapy. A set of protein molecules as potential biomark...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-015-0161-6

    authors: Tsaur I,Thurn K,Juengel E,Gust KM,Borgmann H,Mager R,Bartsch G,Oppermann E,Ackermann H,Nelson K,Haferkamp A,Blaheta RA

    更新日期:2015-05-14 00:00:00

  • Oncogene addiction in gliomas: implications for molecular targeted therapy.

    abstract::Oncogene addiction is a phenomenon that the survival of cancer cells depends on an activated oncogene or inactivation of tumor suppressor gene, and is regarded as the 'Achilles heel' of the successful molecular targeted therapies in cancer. However, the role of oncogene addiction in gliomas has not been elucidated sys...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/1756-9966-30-58

    authors: Yan W,Zhang W,Jiang T

    更新日期:2011-05-17 00:00:00

  • circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma.

    abstract:BACKGROUND:Growing evidences indicate that circular RNAs (circRNAs) play an important role in the regulation of biological behavior of tumor. We aim to explore the role of circRNA in glioma and elucidate how circRNA acts. METHODS:Real-time PCR was used to examine the expression of circPTN in glioma tissues and normal ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1376-8

    authors: Chen J,Chen T,Zhu Y,Li Y,Zhang Y,Wang Y,Li X,Xie X,Wang J,Huang M,Sun X,Ke Y

    更新日期:2019-09-11 00:00:00

  • Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression.

    abstract:BACKGROUND:Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet. MATERIALS AND METHODS:qRT-PCR and western blot were conducted to measure relev...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01551-9

    authors: Rong Z,Wang Z,Wang X,Qin C,Geng W

    更新日期:2020-04-09 00:00:00

  • Breast carcinomas with low amplified/equivocal HER2 by Ish: potential supporting role of multiplex ligation-dependent probe amplification.

    abstract:BACKGROUND:This is a retrospective cross sectional study aimed to verify whether Multiplex Ligation-dependent Probe Amplification (MLPA), a quantitative molecular assay, may represent a valuable reflex test in breast cancer with equivocal HER2 expression by immunohistochemistry and HER2 gene signals/nucleus (s/n) rangi...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-017-0613-2

    authors: Ercolani C,Marchiò C,Di Benedetto A,Fabi A,Perracchio L,Vici P,Sperati F,Buglioni S,Arena V,Pescarmona E,Sapino A,Terrenato I,Mottolese M

    更新日期:2017-10-13 00:00:00

  • Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma.

    abstract:BACKGROUND:Despite the improvement of relapse-free survival mediated by anti-angiogenic drugs like sunitinib (Sutent®), or by combinations of anti-angiogenic drugs with immunotherapy, metastatic clear cell Renal Cell Carcinoma (mccRCC) remain incurable. Hence, new relevant treatments are urgently needed. The VEGFs core...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-021-01832-x

    authors: Dumond A,Brachet E,Durivault J,Vial V,Puszko AK,Lepelletier Y,Montemagno C,Pagnuzzi-Boncompagni M,Hermine O,Garbay C,Lagarde N,Montes M,Demange L,Grépin R,Pagès G

    更新日期:2021-01-18 00:00:00

  • AIDS-Kaposi Sarcoma and Classic Kaposi Sarcoma: are different ultrasound patterns related to different variants?

    abstract:BACKGROUND:Kaposi Sarcoma (KS) is a malignancy of endothelial skin cells with multifocal localization on the skin, lymph nodes and visceral organs. Although all clinical variants are associated with HHV-8 infection, specific differences in the clinical onset and in the natural history of AIDS-KS and Classic-KS have bee...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-30-40

    authors: Solivetti FM,Elia F,Latini A,Cota C,Cordiali-Fei P,Di Carlo A

    更新日期:2011-04-13 00:00:00

  • Correlation effect of EGFR and CXCR4 and CCR7 chemokine receptors in predicting breast cancer metastasis and prognosis.

    abstract:BACKGROUND:The chemokine receptors CXCR4 and CCR7 play an important role in cancer invasion and metastasis. This study investigated the expression of CXCR4, CCR7, CXCL12, CCL21, and EGFR to illustrate the role of these biomarkers in breast cancer metastasis and prognosis. METHODS:The CXCR4, CCR7, CXCL12, CCL21, and EG...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-29-16

    authors: Liu Y,Ji R,Li J,Gu Q,Zhao X,Sun T,Wang J,Li J,Du Q,Sun B

    更新日期:2010-02-24 00:00:00

  • Loss of expression of Kruppel-like factor 6 in primary hepatocellular carcinoma and hepatoma cell lines.

    abstract::KLF6 (Zf9, COPEB), an ubiquitous transcription factor, maps to chromosome 10p. Recently, KLF6 was found to have a more generalized role in tumorigenesis as a candidate tumor suppressor gene for some tumors. However, results from other published studies seem not to be in agreement with data from previous studies. Gene-...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Wang SP,Zhou HJ,Chen XP,Ren GY,Ruan XX,Zhang Y,Zhang RL,Chen J

    更新日期:2007-03-01 00:00:00

  • Inhibition of YAP suppresses CML cell proliferation and enhances efficacy of imatinib in vitro and in vivo.

    abstract:BACKGROUND:Yes-associated protein (YAP), an essential component of Hippo pathway, was identified as an oncoprotein which participated in the progression of various malignancies. However, its role in chronic myeloid leukemia (CML) remains to be further clarified. METHODS:The expression of YAP in CML cells was determine...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-016-0414-z

    authors: Li H,Huang Z,Gao M,Huang N,Luo Z,Shen H,Wang X,Wang T,Hu J,Feng W

    更新日期:2016-09-06 00:00:00

  • The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line.

    abstract::The aim of this work is the in vitro study of the late effects of single proton irradiation on HTB63 human melanoma cell growth, cell cycle and cell death. The experimental conditions were focused on analyzing the effects of irradiation on the periphery of tumour that can be, in clinical practice, close to critical or...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Ristic-Fira A,Nikolic D,Petrovic I,Ruzdijic S,Raffaele L,Sabini MG,Cirrone GA,Cuttone G,Farruggia G,Masotti L,Kanazir DT

    更新日期:2001-03-01 00:00:00

  • External Quality Assessment (EQA) program for the preanalytical and analytical immunohistochemical determination of HER2 in breast cancer: an experience on a regional scale.

    abstract:BACKGROUND:An External Quality Assessment (EQA) program was developed to investigate the state of the art of HER2 immunohistochemical determination in breast cancer (BC) in 16 Pathology Departments in the Lazio Region (Italy). This program was implemented through two specific steps to evaluate HER2 staining (step 1) an...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/1756-9966-32-58

    authors: Terrenato I,Arena V,Pizzamiglio S,Pennacchia I,Perracchio L,Buglioni S,Ercolani C,Sperati F,Costarelli L,Bonanno E,Baldini D,Candia S,Crescenzi A,Dal Mas A,Di Cristofano C,Gomes V,Grillo LR,Pasquini P,Pericoli MN,Ra

    更新日期:2013-08-21 00:00:00

  • Amelioration of cisplatin induced nephrotoxicity in mice by ethyl acetate extract of a polypore fungus, Phellinus rimosus.

    abstract::Pretreatment of ethyl acetate extract of Phellinus rimosus (Berk) Pilat protected cisplatin induced nephrotoxicity in mice. Single dose of the extract (25 and 50 mg/kg body weight) decreased cisplatin induced serum creatinine and urea levels. The cisplatin depleted renal antioxidant defence system, such as activity of...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Ajith TA,Jose N,Janardhanan KK

    更新日期:2002-06-01 00:00:00

  • Mechanisms of hyperprogressive disease after immune checkpoint inhibitor therapy: what we (don't) know.

    abstract::Immune checkpoint inhibitors (ICIs) have made a breakthrough in the treatment of different types of tumors, leading to improvement in survival, even in patients with advanced cancers. Despite the good clinical results, a certain percentage of patients do not respond to this kind of immunotherapy. In addition, in a fra...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-020-01721-9

    authors: Camelliti S,Le Noci V,Bianchi F,Moscheni C,Arnaboldi F,Gagliano N,Balsari A,Garassino MC,Tagliabue E,Sfondrini L,Sommariva M

    更新日期:2020-11-09 00:00:00

  • Gut-endocrinomas (carcinoids and related endocrine variants) of the uterine cervix: an analysis of 205 reported cases.

    abstract::This study was carried out statistically to evaluate the present situation of carcinoids and related endocrine variants in the uterine cervix. A total of 205 patients who had been reported on in world-wide literature were divided into two groups; one, as a carcinoid group, consisting of 81 patients with carcinoids, 49...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Soga J,Osaka M,Yakuwa Y

    更新日期:2001-09-01 00:00:00

  • Non-coding RNAs in cancer: platforms and strategies for investigating the genomic "dark matter".

    abstract::The discovery of the role of non-coding RNAs (ncRNAs) in the onset and progression of malignancies is a promising frontier of cancer genetics. It is clear that ncRNAs are candidates for therapeutic intervention, since they may act as biomarkers or key regulators of cancer gene network. Recently, profiling and sequenci...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-020-01622-x

    authors: Grillone K,Riillo C,Scionti F,Rocca R,Tradigo G,Guzzi PH,Alcaro S,Di Martino MT,Tagliaferri P,Tassone P

    更新日期:2020-06-20 00:00:00

  • Viscum album L. extracts in breast and gynaecological cancers: a systematic review of clinical and preclinical research.

    abstract:BACKGROUND:Viscum album L. extracts (VAE, European mistletoe) are a widely used medicinal plant extract in gynaecological and breast-cancer treatment. METHODS:Systematic review to evaluate clinical studies and preclinical research on the therapeutic effectiveness and biological effects of VAE on gynaecological and bre...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/1756-9966-28-79

    authors: Kienle GS,Glockmann A,Schink M,Kiene H

    更新日期:2009-06-11 00:00:00

  • Pleomorphic adenoma of cervical heterotopic salivary glands.

    abstract::Neoplastic transformation of heterotopic cervical salivary gland tissue is extremely rare. The Authors describe a case of pleomorphic adenoma arising in this tissue, on the right side of the neck in a 40-year old man. The characteristics of salivary heterotipias of the neck, and their diagnostic difficulties and histo...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:

    authors: Baldi A,Persichetti P,Di Marino MP,Nicoletti G,Baldi F

    更新日期:2003-12-01 00:00:00

  • Mucin 17 inhibits the progression of human gastric cancer by limiting inflammatory responses through a MYH9-p53-RhoA regulatory feedback loop.

    abstract:BACKGROUND:Mucins are key components of the mucosal barrier in the stomach that protects epithelia from carcinogenic effects of chronic inflammation. Analysis of The Cancer Genome Atlas database indicated that mucin-17 (MUC17) was more highly expressed in gastric cancer (GC) specimens, with favourable prognosis for pat...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1279-8

    authors: Yang B,Wu A,Hu Y,Tao C,Wang JM,Lu Y,Xing R

    更新日期:2019-07-01 00:00:00

  • Relevance of immune cell and tumor microenvironment imaging in the new era of immunotherapy.

    abstract::Tumor-infiltrating immune cells play a key role against cancer. However, malignant cells are able to evade the immune response and establish a very complex balance in which different immune subtypes may drive tumor progression, metastatization and resistance to therapy. New immunotherapeutic approaches aim at restorin...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-020-01586-y

    authors: Galli F,Aguilera JV,Palermo B,Markovic SN,Nisticò P,Signore A

    更新日期:2020-05-18 00:00:00

  • Long noncoding RNA EPB41L4A-AS2 inhibits hepatocellular carcinoma development by sponging miR-301a-5p and targeting FOXL1.

    abstract:BACKGROUND:Hepatocellular carcinoma (HCC) is the major histological type of liver cancer with high morbidity and mortality worldwide. Long noncoding RNAs (lncRNA) has been proved to be associated with various cancer types, while its regulation in HCC is largely unknown. METHODS:To figure out the specific role of lncRN...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1128-9

    authors: Wang YG,Wang T,Shi M,Zhai B

    更新日期:2019-04-10 00:00:00

  • Annexin A2 contributes to cisplatin resistance by activation of JNK-p53 pathway in non-small cell lung cancer cells.

    abstract:BACKGROUND:Development of resistance to therapy continues to be a serious clinical problem in lung cancer management. We previously identified that Annexin A2 is significantly up-regulated in cisplatin-resistant non-small cell lung cancer (NSCLC) A549/DDP cells. However, the exact function and molecular mechanism of An...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-017-0594-1

    authors: Feng X,Liu H,Zhang Z,Gu Y,Qiu H,He Z

    更新日期:2017-09-08 00:00:00

  • Phosphatidylserine exposing-platelets and microparticles promote procoagulant activity in colon cancer patients.

    abstract:BACKGROUND:Colon cancer is invariably accompanied by altered coagulation activity; however, the precise role of phosphatidylserine (PS) in the hypercoagulable state of colon cancer patients remains unclear. We explored the exposure of PS on platelets and microparticles (MPs), and evaluate its role in procoagulant activ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-016-0328-9

    authors: Zhao L,Bi Y,Kou J,Shi J,Piao D

    更新日期:2016-03-25 00:00:00

  • Challenges and potential of PD-1/PD-L1 checkpoint blockade immunotherapy for glioblastoma.

    abstract::PD-1/PD-L1 checkpoint blockades have achieved significant progress in several kinds of tumours. Pembrolizumab, which targets PD-1, has been approved as a first-line treatment for advanced non-small cell lung cancer (NSCLC) patients with positive PD-L1 expression. However, PD-1/PD-L1 checkpoint blockades have not achie...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章,评审

    doi:10.1186/s13046-019-1085-3

    authors: Wang X,Guo G,Guan H,Yu Y,Lu J,Yu J

    更新日期:2019-02-18 00:00:00

  • Efficacy and safety of ipilimumab in elderly patients with pretreated advanced melanoma treated at Italian centres through the expanded access programme.

    abstract:BACKGROUND:Elderly patients with metastatic melanoma have different disease characteristics and a poorer prognosis than younger patients. Data from clinical trials and expanded access programmes (EAPs) suggest ipilimumab confers a consistent survival benefit and has a similar safety profile across different age groups ...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1186/1756-9966-33-30

    authors: Chiarion Sileni V,Pigozzo J,Ascierto PA,Grimaldi AM,Maio M,Di Guardo L,Marchetti P,de Rosa F,Nuzzo C,Testori A,Cocorocchio E,Bernengo MG,Guida M,Marconcini R,Merelli B,Parmiani G,Rinaldi G,Aglietta M,Grosso M,Queiro

    更新日期:2014-04-04 00:00:00

  • A SUMOylation-dependent HIF-1α/CLDN6 negative feedback mitigates hypoxia-induced breast cancer metastasis.

    abstract:BACKGROUND:We have previously described CLDN6 as a tumor suppressor gene in breast cancer. Here, a new finding is that CLDN6 was upregulated under hypoxia, a commonly recognized factor that promotes tumor metastasis. In this study, we aim to explain this confusing finding and delineate the role of CLDN6 in the breast c...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-020-01547-5

    authors: Jia Y,Guo Y,Jin Q,Qu H,Qi D,Song P,Zhang X,Wang X,Xu W,Dong Y,Liang Y,Quan C

    更新日期:2020-02-24 00:00:00

  • MiR-223-3p functions as a tumor suppressor in lung squamous cell carcinoma by miR-223-3p-mutant p53 regulatory feedback loop.

    abstract:BACKGROUND:MicroRNAs have an important role in diverse biological processes including tumorigenesis. MiR-223 has been reported to be deregulated in several human cancer types. However, its biological role has not been functionally characterized in lung squamous cell carcinoma (LSCC). The following study investigates th...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-019-1079-1

    authors: Luo P,Wang Q,Ye Y,Zhang J,Lu D,Cheng L,Zhou H,Xie M,Wang B

    更新日期:2019-02-12 00:00:00

  • Epigenetic reprogramming of epithelial mesenchymal transition in triple negative breast cancer cells with DNA methyltransferase and histone deacetylase inhibitors.

    abstract:BACKGROUND:Triple negative breast cancer (TNBC) is an aggressive neoplasia with no effective therapy. Our laboratory has developed a unique TNBC cell model presenting epithelial mesenchymal transition (EMT) a process known to be important for tumor progression and metastasis. There is increasing evidence showing that e...

    journal_title:Journal of experimental & clinical cancer research : CR

    pub_type: 杂志文章

    doi:10.1186/s13046-018-0988-8

    authors: Su Y,Hopfinger NR,Nguyen TD,Pogash TJ,Santucci-Pereira J,Russo J

    更新日期:2018-12-14 00:00:00