当前位置： SCI文献检索 > JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH期刊下所有文献 > microRNA-96 promotes occurrence and progression of colorectal cancer via regulation of the AMPKα2-FTO-m6A/MYC axis.

microRNA-96 promotes occurrence and progression of colorectal cancer via regulation of the AMPKα2-FTO-m6A/MYC axis.

Abstract：

BACKGROUND:Colorectal cancer (CRC) is one of the frequently occurred malignancies in the world. To date, several onco-microRNAs (miRNAs or miRs), including miR-96, have been identified in the pathogenesis of CRC. In the present study, we aimed to corroborate the oncogenic effect of miR-96 on CRC and to identify the specific mechanisms related to AMPKα2/FTO/m6A/MYC. METHODS:RT-qPCR and Western blot analysis were performed to examine the expression pattern of miR-96, AMPKα2, FTO and MYC in the clinical CRC tissues and cells. The relationship between miR-96 and AMPKα2 was then predicted using in silico analysis and identified by dual-luciferase reporter assay. Gain- or loss-of-function approaches were manipulated to evaluate the modulatory effects of miR-96, AMPKα2, FTO and MYC on cell growth, cycle progression and apoptosis. The mechanism of FTO-mediated m6A modification of MYC was analyzed via Me-RIP and PAR-CLIP analysis. The mediatory effects of miR-96 antagomir on cancerogenesis were validated in vivo. RESULTS:miR-96, FTO and MYC were upregulated, while AMPKα2 was downregulated in CRC tissues and cells. miR-96 could down-regulate AMPKα2, which led to increased expression of FTO and subsequent upregulated expression of MYC via blocking its m6A modification. This mechanism was involved in the pro-proliferative and anti-apoptotic roles of miR-96 in CRC cells. Besides, down-regulation of miR-96 exerted inhibitory effect on tumor growth in vivo. CONCLUSIONS:Taken together, miR-96 antagomir could potentially retard the cancerogenesis in CRC via AMPKα2-dependent inhibition of FTO and blocking FTO-mediated m6A modification of MYC, highlighting novel mechanisms associated with colorectal cancerogenesis.

journal_name

J Exp Clin Cancer Res

journal_title

Journal of experimental & clinical cancer research : CR

authors

Yue C,Chen J,Li Z,Li L,Chen J,Guo Y

doi

10.1186/s13046-020-01731-7

subject

Has Abstract

pub_date

2020-11-12 00:00:00

pages

240

issue

1

eissn

0392-9078

issn

1756-9966

pii

10.1186/s13046-020-01731-7

journal_volume

39

pub_type

杂志文章

相关文献

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH文献大全
  • MicroRNA-144 inhibits the metastasis of gastric cancer by targeting MET expression.

    abstract：:Gastric cancer (GC) remains one of the most common types of malignant cancer, and the molecular mechanism underlying its metastasis is still largely unclear. MicroRNAs have emerged as important regulators of metastasis because of their ability to act on multiple signaling pathways. In our study, we found that miR-144 ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-015-0154-5

    authors： Liu J,Xue H,Zhang J,Suo T,Xiang Y,Zhang W,Ma J,Cai D,Gu X

    更新日期：2015-04-17 00:00:00

  • Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression.

    abstract：BACKGROUND:Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet. MATERIALS AND METHODS:qRT-PCR and western blot were conducted to measure relev...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-020-01551-9

    authors： Rong Z,Wang Z,Wang X,Qin C,Geng W

    更新日期：2020-04-09 00:00:00

  • UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1.

    abstract：BACKGROUND:Ubiquitin-like protein 4A (UBL4A) plays a significant role in protein metabolism and the maintenance of cellular homeostasis. In cancer, UBL4A represses tumorigenesis and is involved in various signaling pathways. Pancreatic ductal adenocarcinoma (PDAC) is still a major cause of cancer-related death and the ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-019-1278-9

    authors： Chen H,Li L,Hu J,Zhao Z,Ji L,Cheng C,Zhang G,Zhang T,Li Y,Chen H,Pan S,Sun B

    更新日期：2019-07-09 00:00:00

  • The kinase inhibitor D11 induces caspase-mediated cell death in cancer cells resistant to chemotherapeutic treatment.

    abstract：BACKGROUND:Multi-drug resistance and predisposition to metastasize are major clinical problems in cancer treatment. Malignant primary brain tumor and pancreatic cancer are two well-known examples of malignant tumors resistant to conventional therapies where aberrant EGFR-mediated and NF-κB signal transduction pathways ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-015-0234-6

    authors： Guerra B,Fischer M,Schaefer S,Issinger OG

    更新日期：2015-10-20 00:00:00

  • FGFs/FGFRs-dependent signalling in regulation of steroid hormone receptors - implications for therapy of luminal breast cancer.

    abstract：:Stromal stimuli mediated by growth factor receptors, leading to ligand-independent activation of steroid hormone receptors, have long been implicated in development of breast cancer resistance to endocrine therapy. Mutations in fibroblast growth factor receptor (FGFR) genes have been associated with a higher incidence...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：10.1186/s13046-019-1236-6

    authors： Piasecka D,Braun M,Kitowska K,Mieczkowski K,Kordek R,Sadej R,Romanska H

    更新日期：2019-05-29 00:00:00

  • Cost of de novo acute myeloid leukemia induction therapy in adults: analysis of EORTC-GIMEMA AML10 and FLANG regimens.

    abstract：:Since the social and financial impact of AML therapy is becoming more and more relevant we analyzed the cost of induction therapy of two different regimens. The first one is part of the widely employed EORTC-GIMEMA AML-10 and consists often days of therapy. The second (FLANG) is a short (three day), Fludarabine, Ara-C...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Clavio M,Quintino S,Masoudi B,Carrara S,Cerri R,Pierri I,Canepa L,Miglino M,Muner P,Damasio E,Gobbi M

    更新日期：2001-06-01 00:00:00

  • Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL.

    abstract：BACKGROUND:Survival of adults with B-Acute Lymphoblastic Leukemia requires accurate risk stratification of patients in order to provide the appropriate therapy. Contemporary techniques, using clinical and cytogenetic variables are incomplete for prognosis prediction. METHODS:To improve the classification of adult pati...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-017-0506-4

    authors： Cruz-Rodriguez N,Combita AL,Enciso LJ,Raney LF,Pinzon PL,Lozano OC,Campos AM,Peñaloza N,Solano J,Herrera MV,Zabaleta J,Quijano S

    更新日期：2017-02-28 00:00:00

  • Palliative treatment of painful bone metastases from non-Hodgkin lymphoma with disodium pamidronate.

    abstract：:We report a case of a 72-year-old male, with a known history of non-Hodgkin lymphoma of the left tonsil for two years, histologically proved and successfully treated by radical surgical excision in combination with external radiotherapy. He presented with diffuse bone pain the last month, especially at the lower left ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Pistevou-Gombaki K,Eleftheriadis N,Sofroniadis I,Makris P,Kouloulias V

    更新日期：2002-09-01 00:00:00

  • Advances in cancer immunotherapy 2019 - latest trends.

    abstract：:Immunotherapy has become an established pillar of cancer treatment improving the prognosis of many patients with a broad variety of hematological and solid malignancies. The two main drivers behind this success are checkpoint inhibitors (CPIs) and chimeric antigen receptor (CAR) T cells. This review summarizes seminal...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：10.1186/s13046-019-1266-0

    authors： Kruger S,Ilmer M,Kobold S,Cadilha BL,Endres S,Ormanns S,Schuebbe G,Renz BW,D'Haese JG,Schloesser H,Heinemann V,Subklewe M,Boeck S,Werner J,von Bergwelt-Baildon M

    更新日期：2019-06-19 00:00:00

  • Relevance of immune cell and tumor microenvironment imaging in the new era of immunotherapy.

    abstract：:Tumor-infiltrating immune cells play a key role against cancer. However, malignant cells are able to evade the immune response and establish a very complex balance in which different immune subtypes may drive tumor progression, metastatization and resistance to therapy. New immunotherapeutic approaches aim at restorin...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：10.1186/s13046-020-01586-y

    authors： Galli F,Aguilera JV,Palermo B,Markovic SN,Nisticò P,Signore A

    更新日期：2020-05-18 00:00:00

  • Active immunotherapy for cancer patients using tumor lysate pulsed dendritic cell vaccine: a safety study.

    abstract：:Cancer vaccine therapy represents a promising therapeutical option. Consistently, with these new treatment strategies, the use of dendritic cell vaccines is becoming increasingly widespread and currently in the forefront for cancer treatment. The purpose of this study was to evaluate the feasibility and safety of tumo...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Ovali E,Dikmen T,Sonmez M,Yilmaz M,Unal A,Dalbasti T,Kuzeyli K,Erturk M,Omay SB

    更新日期：2007-06-01 00:00:00

  • Formononetin inhibits tumor growth by suppression of EGFR-Akt-Mcl-1 axis in non-small cell lung cancer.

    abstract：BACKGROUND:Epidermal growth factor receptor (EGFR) activating mutations play crucial roles in the tumorigenesis of human non-small cell lung cancer (NSCLC). The mechanism regarding how EGFR signaling regulates myeloid cell leukemia sequence 1 (Mcl-1) protein stability and ubiquitination remains undefined. METHODS:MTS ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-020-01566-2

    authors： Yu X,Gao F,Li W,Zhou L,Liu W,Li M

    更新日期：2020-04-10 00:00:00

  • Mitophagy promotes sorafenib resistance through hypoxia-inducible ATAD3A dependent Axis.

    abstract：BACKGROUND:The identification of novel targets for recovering sorafenib resistance is pivotal for Hepatocellular carcinoma (HCC) patients. Mitophagy is the programmed degradation of mitochondria, and is likely involved in drug resistance of cancer cells. Here, we identified hyperactivated mitophagy is essential for sor...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-020-01768-8

    authors： Wu H,Wang T,Liu Y,Li X,Xu S,Wu C,Zou H,Cao M,Jin G,Lang J,Wang B,Liu B,Luo X,Xu C

    更新日期：2020-12-07 00:00:00

  • Circular RNA circLMO7 acts as a microRNA-30a-3p sponge to promote gastric cancer progression via the WNT2/β-catenin pathway.

    abstract：BACKGROUND:Gastric cancer (GC) is one of the most common malignant tumors worldwide. Currently, the overall survival rate of GC is still unsatisfactory despite progress in diagnosis and treatment. Therefore, studying the molecular mechanisms involved in GC is vital for diagnosis and treatment. CircRNAs, a type of nonco...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-020-01791-9

    authors： Cao J,Zhang X,Xu P,Wang H,Wang S,Zhang L,Li Z,Xie L,Sun G,Xia Y,Lv J,Yang J,Xu Z

    更新日期：2021-01-05 00:00:00

  • Patient-derived xenografts: a relevant preclinical model for drug development.

    abstract：:Identifying appropriate preclinical cancer models remains a major challenge in increasing the efficiency of drug development. A potential strategy to improve patient outcomes could be selecting the 'right' treatment in preclinical studies performed in patient-derived xenografts (PDXs) obtained by direct implants of su...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：10.1186/s13046-016-0462-4

    authors： Pompili L,Porru M,Caruso C,Biroccio A,Leonetti C

    更新日期：2016-12-05 00:00:00

  • Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer patients.

    abstract：BACKGROUND:To determine the expression of bone morphogenetic protein-2 (BMP-2) and its receptors BMPRIA, BMPRIB, and BMPRII in epithelial ovarian cancer (EOC) and to analyze their influence on the prognosis of ovarian cancer patients. METHODS:Semi-quantitative RT-PCR and western blot were applied to detect the express...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/1756-9966-29-85

    authors： Ma Y,Ma L,Guo Q,Zhang S

    更新日期：2010-06-30 00:00:00

  • Long noncoding RNA MAPKAPK5-AS1 promotes colorectal cancer progression by cis-regulating the nearby gene MK5 and acting as a let-7f-1-3p sponge.

    abstract：BACKGROUND:Long noncoding RNAs (lncRNAs) are considered critical regulators in cancers; however, the clinical significance and mechanisms of MAPKAPK5-AS1 (hereinafter referred to as MK5-AS1) in colorectal cancer (CRC) remain mostly unknown. METHODS:In this study, quantitative real-time PCR (qPCR) and western blotting ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-020-01633-8

    authors： Yang T,Chen WC,Shi PC,Liu MR,Jiang T,Song H,Wang JQ,Fan RZ,Pei DS,Song J

    更新日期：2020-07-20 00:00:00

  • Correlation between peri-operative serum lactate levels and outcome in pancreatic resection for pancreatic cancer, preliminary report.

    abstract：:This study was undertaken in order to verify if peri-operative serum lactate level changes, resulting from manipulation of the splanchnic circulation during pancreatectomy, reflected clinical outcome in twenty patients (9 males-11 females age 33 to 76) operated for pancreatic cancer. Lactate levels were evaluated at t...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Gruttadauria S,Marino IR,Vitale CH,Mandala L,Scott VL,Doria C

    更新日期：2002-12-01 00:00:00

  • RNA virus receptor Rig-I monitors gut microbiota and inhibits colitis-associated colorectal cancer.

    abstract：BACKGROUND:Retinoic acid-inducible gene-I (Rig-I) is an intracellular viral RNA receptor, which specifically recognizes double-stranded viral RNA initiating antiviral innate immunity. Increasing evidences showed that Rig-I had broader roles in antibacterial immunity and cancer protection. However, the potential roles a...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-016-0471-3

    authors： Zhu H,Xu WY,Hu Z,Zhang H,Shen Y,Lu S,Wei C,Wang ZG

    更新日期：2017-01-05 00:00:00

  • Stem cells in clinical practice: applications and warnings.

    abstract：UNLABELLED:Stem cells are a relevant source of information about cellular differentiation, molecular processes and tissue homeostasis, but also one of the most putative biological tools to treat degenerative diseases. This review focuses on human stem cells clinical and experimental applications. Our aim is to take a c...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：10.1186/1756-9966-30-9

    authors： Lodi D,Iannitti T,Palmieri B

    更新日期：2011-01-17 00:00:00

  • Direct inhibition of ACTN4 by ellagic acid limits breast cancer metastasis via regulation of β-catenin stabilization in cancer stem cells.

    abstract：BACKGROUND:Pharmacology-based target identification has become a novel strategy leading to the discovery of novel pathological biomarkers. Ellagic acid (EA), a dietary polyphenol compound, exhibits potent anticancer activities; however, the underlying mechanisms remain unclear. The current study sought to determine the...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-017-0635-9

    authors： Wang N,Wang Q,Tang H,Zhang F,Zheng Y,Wang S,Zhang J,Wang Z,Xie X

    更新日期：2017-12-02 00:00:00

  • Detection of CD44 variants in fine needle aspiration biopsies of thyroid tumor by RT-PCR.

    abstract：:Increased expression of CD44 variants is often observed in differentiated thyroid carcinomas. To establish a method for gene diagnosis of thyroid without any additional invasions to the patients, RNA in leftover cells inside the needle used for fine needle aspiration biopsies (FNABs) was extracted to detect the expres...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Takano T,Sumizaki H,Amino N

    更新日期：1997-09-01 00:00:00

  • A dosimetric comparison of different treatment plans of palliative spinal bone irradiation: analysis of dose coverage with respect to ICRU 50 Report.

    abstract：BACKGROUND:This study aimed to analyze three-dimensional (3D) dosimetric data of conventional two-dimensional (2D) palliative spinal bone irradiation using different reference points and treatment plans with respect to the International Commission on Radiation Units and Measurements (ICRU) Report 50. METHODS:Forty-fiv...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/1756-9966-28-2

    authors： Andic F,Baz Cifci S,Ors Y,Niang U,Dirier A,Adli M

    更新日期：2009-01-07 00:00:00

  • Evaluation of naphthalmustine, a nitrogen mustard derivative of naphthalimide as a rationally-designed anticancer agent.

    abstract：:Naphthalmustine, 2-[2-[bis-(2-chloroethyl)amino]ethyl]-1H-benz[de]isoquinoline-1,3-dione (Compound 1) has been synthesized as a rationally designed new anticancer agent from N-(2-bromoethyl)naphthalimide. Its chemical alkylating activity exceeded that of nor-HN2 used as standard compound for comparison. Its antitumour...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Pain A,Samanta S,Dutta S,Saxena AK,Shanmugavel M,Kampasi H,Qazi GN,Sanyal U

    更新日期：2003-09-01 00:00:00

  • Demystifying the mechanistic and functional aspects of p21 gene activation with double-stranded RNAs in human cancer cells.

    abstract：BACKGROUND:The recently identified phenomenon of double-stranded RNA (dsRNA)-mediated gene activation (RNAa) has been studied extensively, as it is present in humans, mice, and Caenorhabditis elegans, suggesting that dsRNA-mediated RNAa is an evolutionarily conserved mechanism. Previous studies have shown that dsP21-32...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-016-0423-y

    authors： Wu HL,Li SM,Hu J,Yu X,Xu H,Chen Z,Ye ZQ

    更新日期：2016-09-17 00:00:00

  • PCBP1 depletion promotes tumorigenesis through attenuation of p27Kip1 mRNA stability and translation.

    abstract：BACKGROUND:Poly C Binding Protein 1 (PCBP1) is an RNA-binding protein that binds and regulates translational activity of subsets of cellular mRNAs. Depletion of PCBP1 is implicated in various carcinomas, but the underlying mechanism in tumorigenesis remains elusive. METHODS:We performed a transcriptome-wide screen to ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-018-0840-1

    authors： Shi H,Li H,Yuan R,Guan W,Zhang X,Zhang S,Zhang W,Tong F,Li L,Song Z,Wang C,Yang S,Wang H

    更新日期：2018-08-07 00:00:00

  • Basigin-mediated redistribution of CD98 promotes cell spreading and tumorigenicity in hepatocellular carcinoma.

    abstract：BACKGROUND:Dysregulated endocytosis of membrane proteins contributes significantly to several hallmarks of cancer. Basigin can enhance cancer progression, but its precise mechanism remains unclear. CD98 promotes cell spreading and tumorigenicity by triggering integrin clustering and enhancing cell adhesion to the extra...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-015-0226-6

    authors： Wu B,Wang Y,Yang XM,Xu BQ,Feng F,Wang B,Liang Q,Li Y,Zhou Y,Jiang JL,Chen ZN

    更新日期：2015-10-06 00:00:00

  • LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett's esophagus?

    abstract：BACKGROUND:Investigation of the expression of an intestinal stem cell marker in esophageal adenocarcinomas (EAC) with and without Barrett's Esophagus (BE), with respect to a cancer stem cell (CSC) hypothesis. MATERIALS AND METHODS:Expression of a putative intestinal stem cell marker LgR5 was analyzed in esophageal can...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/1756-9966-30-23

    authors： von Rahden BH,Kircher S,Lazariotou M,Reiber C,Stuermer L,Otto C,Germer CT,Grimm M

    更新日期：2011-02-23 00:00:00

  • Endoscopic palliative therapy in neoplastic diseases of the esophagus.

    abstract：:Over the past 20 years, fiberoptic endoscopy, followed by video-endoscopy, has taken on an increasingly important role in the diagnostics and therapy for digestive tract diseases. Especially in the field of oncology, endoscopy is fundamental not only for the diagnosis and staging of diseases of the gastroenteric tract...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：

    authors： Casale V,Lapenta R,Gigliozzi A,Villotti G

    更新日期：1999-03-01 00:00:00

  • Effects of silencing of mutant p53 gene in human lung adenocarcinoma cell line Anip973.

    abstract：:The mutational spectrum of p53 gene and the biological effects of mutant p53 protein vary greatly from one type of tumor to another. To investigate the biological effects of mutant p53 gene in human lung adenocarcinoma, mutant p53 was silenced by RNA interference (RNAi) in cell line Anip973. Then, the effects of mutan...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Ma LL,Sun WJ,Wang Zh,Zh GY,Li P,Fu SB

    更新日期：2006-12-01 00:00:00