Abstract:
BACKGROUND:The use of corticosteroids (e.g., dexamethasone) as treatment for tendinopathy has recently been questioned as higher risks for ruptures have been observed clinically. In vitro studies have reported that dexamethasone exposed tendon cells, tenocytes, show reduced cell viability and collagen production. Little is known about the effect of dexamethasone on the characteristics of tenocytes. Furthermore, there are uncertainties about the existence of apoptosis and if the reduction of collagen affects all collagen subtypes. METHODS:We evaluated these aspects by exposing primary tendon cells to dexamethasone (Dex) in concentrations ranging from 1 to 1000 nM. Gene expression of the specific tenocyte markers scleraxis (Scx) and tenomodulin (Tnmd) and markers for other mesenchymal lineages, such as bone (Alpl, Ocn), cartilage (Acan, Sox9) and fat (Cebpα, Pparg) was measured via qPCR. Cell viability and proliferation was calculated using a MTS Assay. Cell death was measured by LDH assay and cleaved caspase-3 using Western Blot. Gene expression of collagen subtypes Col1, Col3 and Col14 was analyzed using qPCR. RESULTS:Stimulation with Dex decreased cell viability and LDH levels. Dex also induced a significant reduction of Scx gene expression and a marked loss of fibroblast like cell shape. The mRNA for all examined collagen subtypes was found to be down-regulated. Among non-tendinous genes only Pparg was significantly increased, whereas Acan, Alpl and Sox9 were reduced. CONCLUSIONS:These results indicate a Dex induced phenotype drift of the tenocytes by reducing scleraxis expression. Reduction of several collagen subtypes, but not cell death, seems to be a feature of Dex induced tissue degeneration.
journal_name
BMC Musculoskelet Disordjournal_title
BMC musculoskeletal disordersauthors
Spang C,Chen J,Backman LJdoi
10.1186/s12891-016-1328-9subject
Has Abstractpub_date
2016-11-10 00:00:00pages
467issue
1issn
1471-2474pii
10.1186/s12891-016-1328-9journal_volume
17pub_type
杂志文章abstract:BACKGROUND:With the relatively high rate of injuries to the lower extremity due to jump-landing movement patterns and the accompanied high costs, there is need for determining potential preventive programs. A program on the intervention of jump-landing technique is possibly an important preventative measure since it ap...
journal_title:BMC musculoskeletal disorders
pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2474-11-281
更新日期:2010-12-13 00:00:00
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更新日期:2018-02-13 00:00:00
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pub_type: 杂志文章,评审
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