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DNA hypomethylation of Synapsin II CpG islands associates with increased gene expression in bipolar disorder and major depression.

Abstract：

BACKGROUND:The Synapsins (SYN1, SYN2, and SYN3) are important players in the adult brain, given their involvement in synaptic transmission and plasticity, as well as in the developing brain through roles in axon outgrowth and synaptogenesis. We and others previously reported gene expression dysregulation, both as increases and decreases, of Synapsins in mood disorders, but little is known about the regulatory mechanisms leading to these differences. Thus, we proposed to study DNA methylation at theses genes' promoter regions, under the assumption that altered epigenetic marks at key regulatory sites would be the cause of gene expression changes and thus part of the mood disorder etiology. METHODS:We performed CpG methylation mapping focusing on the three genes' predicted CpG islands using the Sequenom EpiTYPER platform. DNA extracted from post-mortem brain tissue (BA10) from individuals who had lived with bipolar disorder (BD), major depressive disorder (MDD), as well as psychiatrically healthy individuals was used. Differences in methylation across all CpGs within a CpG island and between the three diagnostic groups were assessed by 2-way mixed model analyses of variance. RESULTS:We found no significant results for SYN1 or SYN3, but there was a significant group difference in SYN2 methylation, as well as an overall pattern of hypomethylation across the CpG island. Furthermore, we found a significant inverse correlation of DNA methylation with SYN2a mRNA expression. CONCLUSIONS:These findings contribute to previous work showing dysregulation of Synapsins, particularly SYN2, in mood disorders and improve our understanding of the regulatory mechanisms that precipitate these changes likely leading to the BD or MDD phenotype.

journal_name

BMC Psychiatry

journal_title

BMC psychiatry

authors

Cruceanu C,Kutsarova E,Chen ES,Checknita DR,Nagy C,Lopez JP,Alda M,Rouleau GA,Turecki G

doi

10.1186/s12888-016-0989-0

subject

Has Abstract

pub_date

2016-08-11 00:00:00

pages

286

issue

issn

1471-244X

pii

10.1186/s12888-016-0989-0

journal_volume

pub_type

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DNA hypomethylation of Synapsin II CpG islands associates with increased gene expression in bipolar disorder and major depression.